Abstract:
:An 8 bp sequence repeated 6 times is present to the right of the mannopine type pRi8196 T-DNA right-border sequence. Experiments were designed to test whether these repeats have a role in T-DNA transfer. Several constructs in which different lengths of pRi8196 right-border region were linked to the cucumopine synthesis gene on an Agrobacterium-Escherichia coli shuttle vector were made. The recombinant plasmids were tested for their efficiency to act as a source of T-DNA in a binary system in which a wild-type Ri plasmid provided virulence and root-inducing functions. The T-DNA transfer efficiency of the constructs was assessed by computing the relative frequency of roots containing cucumopine. Depending on the Ri plasmid used as source of virulence functions, a high level of T-DNA transfer was observed only if 6 (pRi8196) or 5 (pRiA4) repeats were present. These results were confirmed by looking for single-stranded T-DNA molecules (T-strands) in bacteria induced for virulence. The repetition of the 8 bp unit was named 'T-DNA transfer stimulator sequence' (TSS).
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Hansen G,Tempé J,Brevet Jdoi
10.1007/BF00029154subject
Has Abstractpub_date
1992-10-01 00:00:00pages
113-22issue
1eissn
0167-4412issn
1573-5028journal_volume
20pub_type
杂志文章abstract::The entire mitochondrial genome (mt genome) of the unicellular green alga Platymonas subcordiformis (synonym Tetraselmis subcordiformis; Prasinophyceae) was cloned and a physical map for the four restriction enzymes Hind III, Eco RI, Bg/II and Xba I was constructed. The mt genome of P. subcordiformis is a 42.8 kb circ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00014979
更新日期:1995-12-01 00:00:00
abstract::Rice has developed several morphological and physiological strategies to adapt to phosphate starvation in the soil. In order to elucidate the molecular basis of response to phosphate starvation, we performed mRNA sequencing of 4 rice cultivars with variation in growth response to Pi starvation as indicated by the shoo...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0106-4
更新日期:2013-12-01 00:00:00
abstract::Genomic variation in S1 and S2 homologous sequences, defined as the S regions, were examined in mitochondrial DNAs of 12 normal cytoplasm maize lines collected in the United States. Three genomic variants were detected among the 12 cytoplasms, eight of which were identical to the Wf9 model structure. Hybridization dat...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF01578377
更新日期:1983-07-01 00:00:00
abstract::ATP-binding cassette transporters are involved in the active transport of a wide variety of metabolites in prokaryotes and eukaryotes. One subfamily, the Pleiotropic Drug Resistance (PDR) transporters, or full-size ABCG transporters, are found only in fungi and plants. NtPDR1 was originally identified in Nicotiana tab...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0053-0
更新日期:2013-05-01 00:00:00
abstract::Glutelin is the most abundant storage protein in rice, which is expressed specifically in the endosperm of maturing seed. Glutelin is encoded by about 10 genes per haploid genome, which are clearly divided into two subfamilies (GluA and GluB). Most of them are coordinately expressed during seed maturation in spite of ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019553
更新日期:1996-03-01 00:00:00
abstract::Chromosomal non-histone high-mobility-group (HMG) proteins represent essential components of eukaryotic chromatin and have also been isolated from a variety of plants. In maize, studies on structure and function of the two larger of the four major HMG proteins have recently been performed and are now extended by analy...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00043885
更新日期:1994-06-01 00:00:00
abstract::A drought- and abscisic acid (ABA)-inducible gene, His1, was isolated from Lycopersicon pennellii, a drought-resistant relative of cultivated tomato, and the gene structure was defined experimentally. The nucleotide sequence of His1 predicts a protein of 202 amino acid residues, with a significant sequence homology to...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020112
更新日期:1996-01-01 00:00:00
abstract::We report the sequences of full-length cDNAs for the nuclear genes encoding the chloroplastic and cytosolic fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) from spinach. A comparison of the deduced amino-acid sequences with one another and with published cytosolic aldolase sequences of other plants revealed that the ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019948
更新日期:1993-01-01 00:00:00
abstract::Immunofluorescence flow cytometry was used to study the distribution of viral antigen in protoplast populations. Protoplasts were isolated from healthy and alfalfa mosaic virus (AMV) infected tobacco leaves (designated in vivo infected). Furthermore isolated tobacco and cowpea protoplasts were infected in vitro with A...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00187572
更新日期:1983-01-01 00:00:00
abstract::RNA editing of T-urf13, a gene specific to the mitochondria of cytoplasmic male-sterile, type-T (cms-T) maize, and an adjacent, cotranscribed gene orf221, have been studied by cDNA sequencing. No editing was detected in 22 cDNA clones. This is the only report of a polypeptide-encoding gene in higher-plant mitochondria...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00037148
更新日期:1991-11-01 00:00:00
abstract:KEY MESSAGE:Modification of the poplar defense pathway through pathogen-induced expression of an amphibian host defense peptide modulates plant innate immunity and confers robust and reliable resistance against a major poplar pathogen, Septoria musiva. Host defense peptides (HDPs), also known as cationic antimicrobial ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00874-2
更新日期:2019-07-01 00:00:00
abstract::The often rapid and synchronous programmed death of petal cells provides a model system to study molecular aspects of organ senescence. The death of petal cells is preceded by a loss of membrane permeability, due in part to increases in reactive oxygen species that are in turn related to up-regulation of oxidative enz...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1026540524990
更新日期:2000-10-01 00:00:00
abstract::We have analysed the expression of the 8-10 members of the gene family encoding the flavonoid biosynthetic enzyme chalcone synthase (CHS) from Petunia hybrida. During normal plant development only two members of the gene family (CHS-A and CHS-J) are expressed. Their expression is restricted to floral tissues mainly. A...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020506
更新日期:1989-02-01 00:00:00
abstract::Three polypeptides of 33 kD, 23 kD and 16 kD are released from maize photosystem II particles by alkaline Tris solution treatment and shown to cross-react with antisera to purified spinach oxygen-evolving (OE) polypeptides of 34 kD, 23 kD and 17 kD, respectively. They are not located exclusively in mesophyll cells but...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00166458
更新日期:1987-05-01 00:00:00
abstract::Cassava mosaic disease (CMD) is a viral disease of the important tropical staple crop cassava (Manihot esculenta) and preferred management involves use of host-plant resistance. The best available resistance is controlled by a single dominant gene. Serial analysis of gene expression (SAGE) was used to analyze the gene...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-3477-8
更新日期:2004-11-01 00:00:00
abstract::Two subtractive cDNA libraries were developed to study genes associated with bud dormancy (reverse library) and initiation of shoot growth (forward library) in leafy spurge. To identify unique sequences represented in each library, 15744 clones were screened to reduce the level of redundancy within both libraries. A t...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-0015-x
更新日期:2006-05-01 00:00:00
abstract::Despite the presence in nature of many functional gene families that contain several to many highly similar sequences, the presence of identical DNA sequence repeats is widely thought to predispose transgene inserts to homology dependent gene silencing (HDGS). The induction of transcriptional gene silencing (TGS) by R...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-5101-y
更新日期:2005-06-01 00:00:00
abstract:KEY MESSAGE:We developed a machine learning-based model to identify the hidden labels of m6A candidates from noisy m6A-seq data. Peak-calling approaches, such as MeRIP-seq or m6A-seq, are commonly used to map m6A modifications. However, these technologies can only map m6A sites with 100-200 nt resolution and cannot rev...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00930-x
更新日期:2019-12-01 00:00:00
abstract::Plant infections with cassava latent virus (CLV) were mediated by the Ti plasmid of Agrobacterium tumefaciens containing either monomeric or dimeric copies of the virus genome. The CLV DNAs caused typical symptoms when they were inoculated in Agrobacterium strains C58, LBA4404 and a virE mutant A1026, but not other Ag...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019520
更新日期:1988-11-01 00:00:00
abstract::The monoterpene indole alkaloids (MIAs) from Madagascar periwinkle (Catharanthus roseus) are secondary metabolites of high interest due to their therapeutical values. Secologanin, the monoterpenoid moiety incorporated into MIAs, is derived from the plastidial methyl-D: -erythritol 4-phosphate (MEP) pathway. Here, we h...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9190-7
更新日期:2007-09-01 00:00:00
abstract::A cDNA clone was isolated by differential hybridization from a library prepared from barley leaves inoculated with the fungus Blumeria graminis f.sp. hordei (Bgh). The open reading frame of the insert (designated HvCHS2) encoded a polypeptide with 72-79% identity to chalcone synthases (CHS) and 65-68% identity to stil...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006031822141
更新日期:1998-07-01 00:00:00
abstract::Alpha-dioxygenases (α-DOX) catalyzing the primary oxygenation of fatty acids to oxylipins were recently found in plants. Here, the biological roles of the pepper α-DOX (Ca-DOX) gene, which is strongly induced during non-host pathogen infection in chili pepper, were examined. Virus-induced gene silencing demonstrated t...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0575-3
更新日期:2017-03-01 00:00:00
abstract::The expression of chloroplast genes during cotton embryogenesis was followed by measuring hybridization of cloned chloroplast genomic fragments to total RNA immobilzed on membrane filters. RNA was extracted from the cotyledons of cotton embryos at different stages of development ranging from very small embryos (5 mg) ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040137
更新日期:1986-03-01 00:00:00
abstract::In the present study, we utilized TALEN- and CRISPR/Cas9-induced mutations to analyze the promoter of the barley phytase gene HvPAPhy_a. The purpose of the study was dual, validation of the PAPhy_a enzyme as the main contributor of the mature grain phytase activity (MGPA), as well as validating the importance of a spe...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-017-0640-6
更新日期:2017-09-01 00:00:00
abstract::The caffeine biosynthetic pathway in coffee plants has been proposed to involve three distinct N -methyltransferases, xanthosine methyltransferase (XMT), 7- N -methylxanthine methyltransferase (MXMT; theobromine synthase), and 3,7-dimethylxanthine methyltransferase (DXMT; caffeine synthase). We previously isolated all...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-0393-x
更新日期:2004-04-01 00:00:00
abstract::Tomato plants stably expressing the tetR gene were transformed with the beta-glucuronidase (GUS) gene and an le20 antisense construct both under the control of the Triple-Op promoter. Induction of GUS mRNA and le20 antisense RNA in leaves was demonstrated in response to tetracycline. le20 antisense induction was also ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005888616748
更新日期:1997-07-01 00:00:00
abstract::A cDNA containing the entire coding region for the iron storage protein ferritin has been isolated from the French bean plant, Phaseolus vulgaris L. cv. Tendergreen. Ferritin protein was purified from young leaves and shoot meristem tissue and used to raise antisera in mice. A lambda gt11 cDNA library was constructed ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040644
更新日期:1991-09-01 00:00:00
abstract::The nucleotide sequence distal to the rRNA operon from maize chloroplasts has been analyzed. It contains genes coding for tRNA(Arg)(ACG) and tRNA(Asn)(GUU). The tRNA(Arg)(ACG) gene, which is separated from the last rRNA gene of the rRNA operon, the 5S rDNA, by an intergenic region of 252 bp, has the same orientation a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020326
更新日期:1986-11-01 00:00:00
abstract::Exposure of seedlings of a chilling-sensitive variety of rice (Oryza sativa L. cv. Wasetoittu) to water stress (0.5 M mannitol, 30 min) at room temperature induced a degree of chilling resistance. No such resistance was induced by exogenous abscisic acid (ABA) application (10 microM, 60 min). Upon short-term water str...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00039544
更新日期:1994-10-01 00:00:00
abstract::We identified an open reading frame (ORF) which is located closely behind the gene encoding granule-bound starch synthase (GBSS) of potato (Solanum tuberosum L.). The ORF ends with a perfect 43 bp direct repeat, which carries the stop triplet precisely at the beginning of the second repeat. The deduced protein shows h...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028815
更新日期:1993-02-01 00:00:00