Tetracycline-dependent activation of an upstream promoter reveals transcriptional interference between tandem genes within T-DNA in tomato.

Abstract:

:Tomato plants stably expressing the tetR gene were transformed with the beta-glucuronidase (GUS) gene and an le20 antisense construct both under the control of the Triple-Op promoter. Induction of GUS mRNA and le20 antisense RNA in leaves was demonstrated in response to tetracycline. le20 antisense induction was also demonstrated in fruit. Activation of the Triple-Op promoter reduced expression of the downstream hygromycin phosphotransferase II (hptII) gene. Transcriptional interference was therefore observed between tandem genes within T-DNA. Such interference may occur in other T-DNAs, but would not be readily observed without an upstream promoter with controllable activity.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Thompson AJ,Myatt SC

doi

10.1023/a:1005888616748

subject

Has Abstract

pub_date

1997-07-01 00:00:00

pages

687-92

issue

4

eissn

0167-4412

issn

1573-5028

journal_volume

34

pub_type

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