Abstract:
:The single-copy gene encoding the Arabidopsis HMG-I/Y protein was isolated and characterised. The gene encodes a protein of 204 amino acid residues and contains a single intron of 73 bp. Primer extension analysis indicates that transcription starts 115 bp upstream of the translation start and the leader sequence contains a short open reading frame of 13 amino acid residues. The 5'-upstream region of 2117 bp and several 5' deletions were fused to the beta-glucuronidase (GUS) reporter gene and transferred to tobacco by Agrobacterium-mediated transformation. Analysis of transgenic tobacco plants containing HMG-I/Y promoter regions of -2117, -1468 and -707 from the translation start detected GUS activity in all organs examined, including roots, stems, leaves and floral organs. Deletion from -707 to -185 resulted in a 20-30-fold reduction in GUS activity in roots and stems, indicating the presence of important quantitative regulatory elements in this region.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Gupta R,Webster CI,Gray JCdoi
10.1023/a:1005928219895subject
Has Abstractpub_date
1998-04-01 00:00:00pages
897-907issue
6eissn
0167-4412issn
1573-5028journal_volume
36pub_type
杂志文章abstract::Two cDNA clones encoding fucoxanthin chlorophyll a/c-binding proteins (FCP) in the diatom Odontella sinensis have been cloned and sequenced. The derived amino acid sequences of both clones are identical, comparison of the corresponding nucleic acids reveals differences only in the third codon position, suggesting a re...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020236
更新日期:1995-02-01 00:00:00
abstract::Polyphenol oxidases (PPOs) catalyze the oxidation of ortho-diphenols to the corresponding quinones (EC 1.10.3.1). In plants PPOs appear in gene families, and the corresponding isoenzymes are located to the thylakoid lumen of chloroplasts. Although plant PPOs are often discussed with regard to their role in defense rea...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-012-9943-9
更新日期:2012-09-01 00:00:00
abstract::Screening of a lambda gt11 cDNA expression library of Euglena gracilis with antibodies directed against histones H2 from maize resulted in the isolation of a full-length cDNA for a histone H2A. The open-reading frame of 408 bp corresponded to a protein of 136 amino acid residues (14 kDa). Despite the presence of a pol...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00014447
更新日期:1994-03-01 00:00:00
abstract:KEY MESSAGE:OsWRKY36 represses plant height and grain size by inhibiting gibberellin signaling. Plant height and grain size are important agronomic traits affecting yield in cereals, including rice. Gibberellins (GAs) are plant hormones that promote plant growth and developmental processions such as stem elongation and...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01049-0
更新日期:2020-11-01 00:00:00
abstract::Plants count on a wide variety of metabolic, physiological, and developmental responses to adapt their growth to variations in mineral nutrient availability. To react to such variations plants have evolved complex sensing and signaling mechanisms that allow them to monitor the external and internal concentration of ea...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-008-9380-y
更新日期:2009-03-01 00:00:00
abstract::The relative amounts of some chloroplast polypeptides were followed during greening of leaves from three different plant families. Oat, bean and spinach were the representatives of the Gramineae, Leguminosae and Chenopodiaceae, respectively. By using specific antibodies against subunits of the chloroplast protein comp...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF02418259
更新日期:1985-11-01 00:00:00
abstract::A novel rice genomic sequence encoding coding segments homologous to other metallothionein-like genes was isolated from Oryza sativa genomic library. This sequence, hereby designated as rgMT (rice genomic metallothionein-like gene), consists of two exons and one intron. From the coding sequence, it is predicted that r...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020388
更新日期:1995-06-01 00:00:00
abstract:KEY MESSAGE:MdMYB16 forms homodimers and directly inhibits anthocyanin synthesis via its C-terminal EAR repressor. It weakened the inhibitory effect of MdMYB16 on anthocyanin synthesis when overexpressing MdbHLH33 in callus overexpressing MdMYB16. MdMYB16 could interact with MdbHLH33. Anthocyanins are strong antioxidan...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-017-0601-0
更新日期:2017-05-01 00:00:00
abstract::RNA silencing is a sequence-specific RNA degradation mechanism found in most eukaryotes, where small cleavage products (siRNAs) of double stranded RNA (dsRNA) mediate silencing of genes with sequence identity to the dsRNA inducer. In several systems, silencing has been found to spread from the dsRNA inducer sequence i...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-7307-4
更新日期:2005-07-01 00:00:00
abstract::Unicellular algae grow well under limiting CO2 conditions, aided by a carbon concentrating mechanism (CCM). In C. reinhardtii, this mechanism is inducible and is present only in cells grown under low CO2 conditions. We constructed a cDNA library from cells adapting to low CO2, and screened the library for cDNAs specif...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00021807
更新日期:1996-05-01 00:00:00
abstract::Higher-plant, green and red algal chloroplasts are surrounded by a double membrane envelope. The glaucocystophyte plastid (cyanelle) has retained a prokaryotic cell wall between the two envelope membranes. The complex chloroplasts of Euglena and dinoflagellates are surrounded by three membranes while the complex chlor...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:
更新日期:1998-09-01 00:00:00
abstract:KEY MESSAGE:An exhaustive analysis of a group of closely related parasitic plants shows a predominantly gradual reduction in plastid genome composition and provides the most reduced plastomes in the genus Cuscuta. Parasitic plants have a diminished to completely absent reliance on photosynthesis, and are characterized ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00884-0
更新日期:2019-08-01 00:00:00
abstract::Soybean is an important crop not only for human consumption but also for its addition of nitrogen to the soil during crop rotation. China has the largest collection of cultivated soybeans (Glycine max) and wild soybeans (Glycine soja) all over the world. The platform of soybean core, mini core and integrated applied c...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-013-0076-6
更新日期:2013-09-01 00:00:00
abstract::Recent studies have revealed high sequence similarity between homospermidine synthase (HSS), the first pathway-specific enzyme in the biosynthesis of pyrrolizidine alkaloids, a class of sporadically occurring plant defence compounds, and deoxyhypusine synthase (DHS), a ubiquitous enzyme involved in the post-translatio...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1026597621646
更新日期:2000-11-01 00:00:00
abstract::H(+)-ATPase cDNAs were identified in a potato leaf library using an Arabidopsis gene as a probe. Based on their sequences, the clones could be grouped into at least two classes. A similar classification was obtained from the analysis of sequence data from four tobacco genes. Both potato genes are expressed in all tiss...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028864
更新日期:1994-11-01 00:00:00
abstract::The phytohormone abscisic acid is probably present in all higher plants. This hormone is necessary for regulation of several events during seed development and for the response to environmental stresses such as desiccation, salt and cold. An important part of the physiological response to abscisic acid is achieved thr...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1006058700720
更新日期:1998-06-01 00:00:00
abstract::In the present study, we utilized TALEN- and CRISPR/Cas9-induced mutations to analyze the promoter of the barley phytase gene HvPAPhy_a. The purpose of the study was dual, validation of the PAPhy_a enzyme as the main contributor of the mature grain phytase activity (MGPA), as well as validating the importance of a spe...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-017-0640-6
更新日期:2017-09-01 00:00:00
abstract::Expression of the light-harvesting complex protein genes (Lhc) is under the control of a circadian clock. To dissect the molecular regulatory components of the circadian clock a promoter deletion analysis of four tomato Lhc genes was performed in transgenic tobacco plants. The important 5'-upstream promoter regions ar...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006094015513
更新日期:1998-11-01 00:00:00
abstract:KEY MESSAGE:We identified three dormant stages of Polygonatum kingianum and changes that occurred during dormancy transition in the following aspects including cell wall and hormones, as well as interaction among them. Polygonatum kingianum Coll.et Hemsl (P. kingianum) is an important traditional Chinese medicine, but ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-018-0812-z
更新日期:2019-02-01 00:00:00
abstract:KEY MESSAGE:Degradation of nitrogen-rich purines is tightly and oppositely regulated under drought and low nitrogen supply in bread wheat. Allantoin is a key target metabolite for improving nitrogen homeostasis under stress. The metabolite allantoin is an intermediate of the catabolism of purines (components of nucleot...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00831-z
更新日期:2019-03-01 00:00:00
abstract::A vector has been designed that contains a truncated CaMV (cauliflower mosaic virus) 35S promoter fused to a receptor gene encoding beta-glucuronidase (GUS), placed adjacent to the left border sequence of an Agrobacterium vector. In potato plants transformed with this vector, different patterns of transcription were d...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020557
更新日期:1991-02-01 00:00:00
abstract::Green cybrids with a new nucleus-chloroplast combination cannot be selected after protoplast fusion in the intersubfamilial Nicotiana-Solanum combination. As an approach to overcome the supposed plastomegenome incompatibility, a partial plastome transfer by genetic recombination has been considered. After fusions of p...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017450
更新日期:1989-01-01 00:00:00
abstract::Elucidating the regulatory mechanisms of plant organ formation is an important component of plant developmental biology and will be useful for crop improvement applications. Plant organ formation, or organogenesis, occurs when a group of primordial cells differentiates into an organ, through a well-orchestrated series...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-0054-3
更新日期:2006-08-01 00:00:00
abstract::We have sequenced a plastid gene cluster from the unicellular red alga Cyanidium caldarium which is located downstream from the psbA gene and contains, in the following order, genes for a beta-allophycocyanin-like protein (apcB'), a putative 9.5 kDa allophycocyanin linker protein (apcL9.5) and a putative 29 kDa phycoc...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00014494
更新日期:1992-10-01 00:00:00
abstract::Alterations in gene expression during early stages of dormancy release in grapevine buds were analyzed to facilitate the identification of gene products that may mediate the signal transduction of a dormancy-release signal, or derepression of meristematic activity. In the present report we describe the identification ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006450516982
更新日期:2000-07-01 00:00:00
abstract::We previously identified a cluster of d ormancy-a ssociated M ADS-box transcription factors (DAM genes) in peach [Prunus persica (L.) Batsch] as potential candidates for control of the non-dormant phenotype observed in the evg mutant. Of these genes, DAM3, DAM5 and DAM6 were winter expressed, suggesting a role for the...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9608-5
更新日期:2010-05-01 00:00:00
abstract::A full-length cDNA encoding the 206 amino acid open reading frame of a trypsin/chymotrypsin inhibitor abundant in the corms of giant taro (Alocasia macrorrhiza) was isolated. An internal fragment was cloned using degenerate primers corresponding to a region of the mature protein sequence and the 'rapid amplification o...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020813
更新日期:1996-03-01 00:00:00
abstract::Plant cells respond to cold stress via a regulatory mechanism leading to enhanced cold acclimation accompanied by growth retardation. The C-repeat binding factor (CBF) signaling pathway is essential for cold response of flowering plants. Our previously study documented a novel CBF-like gene from the cold-tolerant Caps...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0181-1
更新日期:2014-06-01 00:00:00
abstract:KEY MESSAGE:We reported the functional characterization of cDNAs encoding short-chain isoprenyl diphosphate synthases that control the partitioning of precursors for lavender terpenoids. Lavender essential oil is composed of regular and irregular monoterpenes, which are derived from linear precursors geranyl diphosphat...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-00962-8
更新日期:2020-03-01 00:00:00
abstract::Plants that utilize the highly efficient C4 photosynthetic pathway possess two types of specialized leaf cells, the mesophyll and bundle sheath. In mature leaves of amaranth, a dicotyledonous C4 plant, ribulose 1,5-bisphosphate carboxylase (Rubisco) is localized specifically to the chloroplasts of bundle sheath cells,...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015971
更新日期:1993-06-01 00:00:00