Small grain and semi-dwarf 3, a WRKY transcription factor, negatively regulates plant height and grain size by stabilizing SLR1 expression in rice.

Abstract:

KEY MESSAGE:OsWRKY36 represses plant height and grain size by inhibiting gibberellin signaling. Plant height and grain size are important agronomic traits affecting yield in cereals, including rice. Gibberellins (GAs) are plant hormones that promote plant growth and developmental processions such as stem elongation and grain size. WRKYs are transcription factors that regulate stress tolerance and plant development including height and grain size. However, the relationship between GA signaling and WRKY genes is still poorly understood. Here, we characterized a small grain and semi-dwarf 3 (sgsd3) mutant in rice cv. Hwayoung (WT). A T-DNA insertion in the 5'-UTR of OsWRKY36 induced overexpression of OsWRKY36 in the sgsd3 mutant, likely leading to the mutant phenotype. This was confirmed by the finding that overexpression of OsWRKY36 caused a similar small grain and semi-dwarf phenotype to the sgsd3 mutant whereas knock down and knock out caused larger grain phenotypes. The sgsd3 mutant was also hyposensitive to GA and accumulated higher mRNA and protein levels of SLR1 (a GA signaling DELLA-like inhibitor) compared with the WT. Further assays showed that OsWRKY36 enhanced SLR1 transcription by directly binding to its promoter. In addition, we found that OsWRKY36 can protect SLR1 from GA-mediated degradation. We thus identified a new GA signaling repressor OsWRKY36 that represses GA signaling through stabilizing the expression of SLR1.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Lan J,Lin Q,Zhou C,Ren Y,Liu X,Miao R,Jing R,Mou C,Nguyen T,Zhu X,Wang Q,Zhang X,Guo X,Liu S,Jiang L,Wan J

doi

10.1007/s11103-020-01049-0

subject

Has Abstract

pub_date

2020-11-01 00:00:00

pages

429-450

issue

4-5

eissn

0167-4412

issn

1573-5028

pii

10.1007/s11103-020-01049-0

journal_volume

104

pub_type

杂志文章
  • Tissue-specific expression of an oat 12S seed globulin gene in developing tobacco seeds: differential mRNA and protein accumulation.

    abstract::We studied the expression of the oat globulin gene asglo5 in developing transgenic tobacco seeds. The asglo5 gene promoter directed transcription in the endosperm as well as in the provascular tissue, the presumptive root tip and the shoot apical meristem of the embryo as revealed by GUS reporter gene constructs and i...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00039532

    authors: Schubert R,Panitz R,Manteuffel R,Nagy I,Wobus U,Bäumlein H

    更新日期:1994-10-01 00:00:00

  • The essential role of GhPEL gene, encoding a pectate lyase, in cell wall loosening by depolymerization of the de-esterified pectin during fiber elongation in cotton.

    abstract::Cotton fiber elongation, largely achieved by cell wall loosening, is an important stage during cotton fiber development. In this present research, a fiber preferential cDNA encoding a pectate lyase (PEL) which could exclusively degrade the de-esterified pectin was isolated from a cotton (Gossypium hirsutum) fiber cDNA...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-009-9578-7

    authors: Wang H,Guo Y,Lv F,Zhu H,Wu S,Jiang Y,Li F,Zhou B,Guo W,Zhang T

    更新日期:2010-03-01 00:00:00

  • Molecular analysis of early rice stamen development using organ-specific gene expression profiling.

    abstract::Elucidating the regulatory mechanisms of plant organ formation is an important component of plant developmental biology and will be useful for crop improvement applications. Plant organ formation, or organogenesis, occurs when a group of primordial cells differentiates into an organ, through a well-orchestrated series...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-006-0054-3

    authors: Lu XC,Gong HQ,Huang ML,Bai SL,He YB,Mao X,Geng Z,Li SG,Wei L,Yuwen JS,Xu ZH,Bai SN

    更新日期:2006-08-01 00:00:00

  • Sequence-guided approach to genotyping plant clones and species using polymorphic NB-ARC-related genes.

    abstract:KEY MESSAGE:Leveraging the heightened levels of polymorphism in NB-ARC-related protein encoding genes in higher plants, a bioinformatic pipeline was created to identify regions in this gene family from sequenced plant genomes that exhibit fragment length or single nucleotide differences in different accessions of the s...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-018-0774-1

    authors: Chu P,Wilson GM,Michael TP,Vaiciunas J,Honig J,Lam E

    更新日期:2018-10-01 00:00:00

  • Molecular cloning and functional characterization of two kinds of betaine-aldehyde dehydrogenase in betaine-accumulating mangrove Avicennia marina (Forsk.) Vierh.

    abstract::Glycinebetaine is an important osmoprotectant in bacteria, plants, and animals, but only little information is available on the synthesis of glycinebetaine in tree plants. Among four mangrove species, glycinebetaine could be detected only in Avicennia marina. Pinitol was the main osmoprotectant in the other three spec...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006497113323

    authors: Hibino T,Meng YL,Kawamitsu Y,Uehara N,Matsuda N,Tanaka Y,Ishikawa H,Baba S,Takabe T,Wada K,Ishii T,Takabe T

    更新日期:2001-02-01 00:00:00

  • Nucleotide sequence of diatom plasmids: identification of open reading frames with similarity to site-specific recombinases.

    abstract::We have determined the nucleotide sequence of two small circular DNA plasmids, pCf1 and pCf2 [22], from the marine diatom Cylindrotheca fusiformis. pCf1 is 4273 bp, and pCf2 is 4079 bp in size. In each plasmid, all of the major open reading frames (ORFs) are encoded on the same DNA strand. Two ORFs are similar, compar...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00027072

    authors: Hildebrand M,Hasegawa P,Ord RW,Thorpe VS,Glass CA,Volcani BE

    更新日期:1992-08-01 00:00:00

  • The DNA-compacting protein DCP68 from soybean chloroplasts is ferredoxin:sulfite reductase and co-localizes with the organellar nucleoid.

    abstract::The multiple copies of the chloroplast genome (plastome) are condensed and organized into nucleoids by a set of proteins. One of these, the DNA-binding protein DCP68 from soybean, has previously been shown to compact DNA and to inhibit DNA synthesis in vitro. N-terminal amino acid analysis and the absorption spectrum ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1015500431421

    authors: Chi-Ham CL,Keaton MA,Cannon GC,Heinhorst S

    更新日期:2002-08-01 00:00:00

  • An alfalfa mosaic virus RNA 2 mutant, which does not induce a hypersensitive reaction in cowpea plants, is multiplied to a high concentration in cowpea protoplasts.

    abstract::A mutant of alfalfa mosaic virus (AMV), which in contrast to wild type (wt) can invade cowpea plants systemically, is replicated more efficiently in cowpea protoplasts than the wt. Mutant preparations isolated from infected cowpea protoplasts contained a higher amount of middle component (M, containing RNA 2) than wt ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF01595169

    authors: Roosien J,Sarachu AN,Alblas F,Van Vloten-Doting L

    更新日期:1983-03-01 00:00:00

  • Functional analysis of the promoter region of a maize (Zea mays L.) H3 histone gene in transgenic Arabidopsis thaliana.

    abstract::A 1023 bp fragment and truncated derivatives of the maize (Zea mays L.) histone H3C4 gene promoter were fused to the beta-glucuronidase (GUS) gene and introduced via Agrobacterium tumefaciens into the genome of Arabidopsis thaliana. GUS activity was found in various meristems of transgenic plants as for other plant hi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1005909703600

    authors: Atanassova R,Flénet M,Gigot C,Chaubet N

    更新日期:1998-05-01 00:00:00

  • RdDM pathway components differentially modulate Tobamovirus symptom development.

    abstract:KEY MESSAGE:The crop yield losses induced by phytoviruses are mainly associated with the symptoms of the disease. DNA modifications as methylation can modulate the information coded by the sequence, process named epigenetics. Viral infection can change the expression patterns of different genes linked to defenses and s...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-020-01051-6

    authors: Leone M,Zavallo D,Venturuzzi A,Asurmendi S

    更新日期:2020-11-01 00:00:00

  • Induction of chilling resistance by water stress, and cDNA sequence analysis and expression of water stress-regulated genes in rice.

    abstract::Exposure of seedlings of a chilling-sensitive variety of rice (Oryza sativa L. cv. Wasetoittu) to water stress (0.5 M mannitol, 30 min) at room temperature induced a degree of chilling resistance. No such resistance was induced by exogenous abscisic acid (ABA) application (10 microM, 60 min). Upon short-term water str...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00039544

    authors: Takahashi R,Joshee N,Kitagawa Y

    更新日期:1994-10-01 00:00:00

  • cDNA cloning of two isoforms of ornithine carbamoyltransferase from Canavalia lineata leaves and the effect of site-directed mutagenesis of the carbamoyl phosphate binding site.

    abstract::The immunoscreening method was used to isolate cDNAs of 1323 bp (ClOCT1) and 1433 bp (ClOCT2) encoding two ornithine carbamoyltransferases (OCT, EC 2.1.3.3) from the cDNA expression library of Canavalia lineata leaves constructed in a lambdaZAP Express vector. ClOCT1 and ClOCT2 encode 359 and 369 amino acids, respecti...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1011632927541

    authors: Lee Y,Choi YA,Hwang ID,Kim SG,Kwon YM

    更新日期:2001-08-01 00:00:00

  • Cyclophilins are encoded by a small gene family in rice.

    abstract::cDNA clones were isolated and sequenced that encode two related but distinct rice cyclophilins, Cyp1 and Cyp2. The predicted amino acid sequences of each are 72% identical to human T-cell cyclophilin. Genomic DNA gel blot analysis suggests cyclophilins in rice are encoded by a small, 6-10-member gene family. Both Cyp1...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00028878

    authors: Buchholz WG,Harris-Haller L,DeRose RT,Hall TC

    更新日期:1994-08-01 00:00:00

  • Transgene-mediated auxin overproduction in Arabidopsis: hypocotyl elongation phenotype and interactions with the hy6-1 hypocotyl elongation and axr1 auxin-resistant mutants.

    abstract::Transgenic Arabidopsis thaliana plants constitutively expressing Agrobacterium tumefaciens tryptophan monooxygenase (iaaM) were obtained and characterized. Arabidopsis plants expressing iaaM have up to 4-fold higher levels of free indole-3-acetic acid (IAA) and display increased hypocotyl elongation in the light. This...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00020881

    authors: Romano CP,Robson PR,Smith H,Estelle M,Klee H

    更新日期:1995-03-01 00:00:00

  • Temporally regulated expression of a yeast invertase in potato tubers allows dissection of the complex metabolic phenotype obtained following its constitutive expression.

    abstract::The constitutive cytosolic expression of a yeast ( Saccharomyces cerevisiae ) invertase within potato ( Solanum tuberosum ) tubers has previously been documented to produce a dramatic metabolic phenotype in which glycolysis, respiration and amino acid synthesis are markedly enhanced at the cost of starch synthesis. Th...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-004-2525-8

    authors: Junker BH,Wuttke R,Tiessen A,Geigenberger P,Sonnewald U,Willmitzer L,Fernie AR

    更新日期:2004-09-01 00:00:00

  • Arabidopsis thaliana contains a large family of germin-like proteins: characterization of cDNA and genomic sequences encoding 12 unique family members.

    abstract::We have identified 39 Arabidopsis thaliana ESTs encoding germin-like proteins (GLPs) and have completely sequenced 25 of these cDNAs. Our analysis demonstrates that the Arabidopsis genome contains a gene family with at least 12 GLP genes. Comparisons with other known germins and germin-like proteins indicate that thes...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006038117130

    authors: Carter C,Graham RA,Thornburg RW

    更新日期:1998-12-01 00:00:00

  • Oryza sativa heat-induced RING finger protein 1 (OsHIRP1) positively regulates plant response to heat stress.

    abstract:KEY MESSAGE:OsHIRP1 is an E3 ligase that acts as a positive regulator in the plant response to heat stress, thus providing important information relating to adaptation and regulation under heat stress in plant. Extreme temperature adversely affects plant growth, development, and productivity. Here, we report the molecu...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-019-00835-9

    authors: Kim JH,Lim SD,Jang CS

    更新日期:2019-04-01 00:00:00

  • Phytochrome types in Picea and Pinus. Expression patterns of PHYA-Related types.

    abstract::Knowledge of the genes in gymnosperms encoding the apoproteins of the plant photoreceptor phytochrome is currently scanty as for gymnosperm nuclear protein coding sequences in general. Here we report two complete cDNA-derived sequences which code for two different types of gymnosperm phytochrome. One sequence stems fr...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006204318499

    authors: Clapham DH,Kolukisaoglu HU,Larsson CT,Qamaruddin M,Ekberg I,Wiegmann-Eirund C,Schneider-Poetsch HA,von Arnold S

    更新日期:1999-07-01 00:00:00

  • Cassava latent virus infections mediated by the Ti plasmid of Agrobacterium tumefaciens containing either monomeric or dimeric viral DNA.

    abstract::Plant infections with cassava latent virus (CLV) were mediated by the Ti plasmid of Agrobacterium tumefaciens containing either monomeric or dimeric copies of the virus genome. The CLV DNAs caused typical symptoms when they were inoculated in Agrobacterium strains C58, LBA4404 and a virE mutant A1026, but not other Ag...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00019520

    authors: Morris BA,Richardson KA,Andersen MT,Gardner RC

    更新日期:1988-11-01 00:00:00

  • Import of the precursor of the chloroplast Rieske iron-sulphur protein by pea chloroplasts.

    abstract::cDNA clones encoding the precursor of the Rieske FeS protein of the chloroplast thylakoid membrane have been isolated from a pea leaf cDNA library in lambda gt 11, following screening with antibodies to purified pea chloroplast Rieske FeS protein. The longest cDNA insert of 880 bp encodes a polypeptide of 230 amino ac...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00040617

    authors: Salter AH,Newman BJ,Napier JA,Gray JC

    更新日期:1992-11-01 00:00:00

  • Characterization of a mRNA that accumulates during development of oilseed rape pods.

    abstract::Dehiscence of oilseed rape pods, commonly known as pod shatter, is a process of agronomic importance that results in seed loss causing yield reductions and carry-over of the crop into the following growing season. In an effort to understand the mechanisms underlying this developmental event, the changes in gene expres...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00040589

    authors: Coupe SA,Taylor JE,Isaac PG,Roberts JA

    更新日期:1994-01-01 00:00:00

  • Normal and lysine-containing zeins are unstable in transgenic tobacco seeds.

    abstract::Chimeric genes composed of the beta-phaseolin promoter, an alpha-zein coding sequence and its modified versions containing lysine codons, and a beta-zein polyadenylation signal were inserted into the genome of tobacco by Agrobacterium-mediated transformation. alpha-Zein mRNA levels in the transgenic tobacco seeds 20 d...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00017922

    authors: Ohtani T,Galili G,Wallace JC,Thompson GA,Larkins BA

    更新日期:1991-01-01 00:00:00

  • Genetic aspects of the organization of legumin genes in pea.

    abstract::We have compared physical and genetic maps of the region around the legJ gene in pea. In this vicinity there are four B-type legumin genes, arranged as two close pairs. The detection of a recombination event within this gene cluster allows the orientation of this group of genes within the surrounding linkage group to ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00038999

    authors: Turner L,Hellens RP,Lee D,Ellis TH

    更新日期:1993-04-01 00:00:00

  • Cauliflower Mosaic Virus replication complexes: characterization of the associated enzymes and of the polarity of the DNA synthesized in vitro.

    abstract::The synthesis of both strands of CaMV-DNA has been studied in vitro using viral replication complexes obtained by hypotonic extraction of infected plant organelles. Hybridization of the DNA synthesized in vitro to single stranded CaMV DNA probes cloned in bacteriophage M 13 confirmed that the 35 S RNA served as a temp...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00017779

    authors: Pfeiffer P,Laquel P,Hohn T

    更新日期:1984-09-01 00:00:00

  • A novel mitochondrial orf147 causes cytoplasmic male sterility in pigeonpea by modulating aberrant anther dehiscence.

    abstract:KEY MESSAGE:A novel open reading frame (ORF) identified and cloned from the A4 cytoplasm of Cajanus cajanifolius induced partial to complete male sterility when introduced into Arabidopsis and tobacco. Pigeonpea (Cajanus cajan L. Millsp.) is the only legume known to have commercial hybrid seed technology based on cytop...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-018-0728-7

    authors: Bhatnagar-Mathur P,Gupta R,Reddy PS,Reddy BP,Reddy DS,Sameerkumar CV,Saxena RK,Sharma KK

    更新日期:2018-05-01 00:00:00

  • Molecular biology of fruit ripening and its manipulation with antisense genes.

    abstract::Considerable progress in tomato molecular biology has been made over the past five years. At least 19 different mRNAs which increase in amount during tomato fruit ripening have been cloned and genes for enzymes involved in cell wall degradation (polygalacturonase and pectinesterase) and ethylene synthesis (ACC synthas...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审

    doi:10.1007/BF00015607

    authors: Gray J,Picton S,Shabbeer J,Schuch W,Grierson D

    更新日期:1992-05-01 00:00:00

  • The isolation of a novel metallothionein-related cDNA expressed in somatic and zygotic embryos of Douglas-fir: regulation by ABA, osmoticum, and metal ions.

    abstract::To isolate genes which are expressed preferentially during embryogenesis, a Douglas-fir embryogenesis cDNA library was constructed and differentially screened with cDNA probes made with mRNA from developing and mature embryos, respectively. The cDNA clone PM 2.1 was isolated based on its abundance in developing seeds ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1005839832096

    authors: Chatthai M,Kaukinen KH,Tranbarger TJ,Gupta PK,Misra S

    更新日期:1997-05-01 00:00:00

  • Two CYP71AJ enzymes function as psoralen synthase and angelicin synthase in the biosynthesis of furanocoumarins in Peucedanum praeruptorum Dunn.

    abstract:KEY MESSAGE:Psoralen synthase and angelicin synthase responsible for the formation of psoralen and angelicin in Peucedanum praeruptorum Dunn were identified and functionally characterized, respectively. Furanocoumarins were reported to possess several activities such as anticancer, anti-inflammatory and neuroprotective...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-020-01045-4

    authors: Jian X,Zhao Y,Wang Z,Li S,Li L,Luo J,Kong L

    更新日期:2020-10-01 00:00:00

  • The dsRNA-binding protein DRB4 interacts with the Dicer-like protein DCL4 in vivo and functions in the trans-acting siRNA pathway.

    abstract::Arabidopsis thaliana encodes four Dicer-like (DCL) proteins and five dsRNA-binding (DRB) proteins. We have previously demonstrated that DCL4 specifically interacts with DRB4 in vitro. Here we describe the interaction between DCL4 and DRB4 in vivo. The phenotype of a mutant with a defect in DCL4 (dcl4-2) was similar to...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-006-9125-8

    authors: Nakazawa Y,Hiraguri A,Moriyama H,Fukuhara T

    更新日期:2007-04-01 00:00:00

  • Response diversity of Arabidopsis thaliana ecotypes in elevated [CO2] in the field.

    abstract::Free Air [CO(2)] Enrichment (FACE) allows for plant growth under fully open-air conditions of elevated [CO(2)] at concentrations expected to be reached by mid-century. We used Arabidopsis thaliana ecotypes Col-0, Cvi-0, and WS to analyze changes in gene expression and metabolite profiles of plants grown in "SoyFACE" (...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-006-9041-y

    authors: Li P,Sioson A,Mane SP,Ulanov A,Grothaus G,Heath LS,Murali TM,Bohnert HJ,Grene R

    更新日期:2006-11-01 00:00:00