Abstract:
KEY MESSAGE:A novel open reading frame (ORF) identified and cloned from the A4 cytoplasm of Cajanus cajanifolius induced partial to complete male sterility when introduced into Arabidopsis and tobacco. Pigeonpea (Cajanus cajan L. Millsp.) is the only legume known to have commercial hybrid seed technology based on cytoplasmic male sterility (CMS). We identified a novel ORF (orf147) from the A4 cytoplasm of C. cajanifolius that was created via rearrangements in the CMS line and co-transcribes with the known and unknown sequences. The bi/poly-cistronic transcripts cause gain-of-function variants in the mitochondrial genome of CMS pigeonpea lines having distinct processing mechanisms and transcription start sites. In presence of orf147, significant repression of Escherichia coli growth indicated its toxicity to the host cells and induced partial to complete male sterility in transgenic progenies of Arabidopsis thaliana and Nicotiana tabacum where phenotype co-segregated with the transgene. The male sterile plants showed aberrant floral development and reduced lignin content in the anthers. Gene expression studies in male sterile pigeonpea, Arabidopsis and tobacco plants confirmed down-regulation of several anther biogenesis genes and key genes involved in monolignol biosynthesis, indicative of regulation of retrograde signaling. Besides providing evidence for the involvement of orf147 in pigeonpea CMS, this study provides valuable insights into its function. Cytotoxicity and aberrant programmed cell death induced by orf147 could be important for mechanism underlying male sterility that offers opportunities for possible translation for these findings for exploiting hybrid vigor in other recalcitrant crops as well.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Bhatnagar-Mathur P,Gupta R,Reddy PS,Reddy BP,Reddy DS,Sameerkumar CV,Saxena RK,Sharma KKdoi
10.1007/s11103-018-0728-7subject
Has Abstractpub_date
2018-05-01 00:00:00pages
131-147issue
1-2eissn
0167-4412issn
1573-5028pii
10.1007/s11103-018-0728-7journal_volume
97pub_type
杂志文章abstract::We have characterized the topoisomerase I and II activities in nuclear extracts from immature embryos of Zea mays and the effect of the treatment with 2,4-dichlorophenoxyacetic acid (2,4-D) and abscisic acid (ABA). These extracts were shown to be essentially devoid of protease and nuclease activities and they were tes...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017917
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
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doi:10.1007/BF00040137
更新日期:1986-03-01 00:00:00
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-012-9898-x
更新日期:2012-09-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章,评审
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更新日期:2000-06-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00041405
更新日期:1996-12-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:
更新日期:1997-09-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1994-01-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00036917
更新日期:1990-08-01 00:00:00
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更新日期:1995-12-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9441-2
更新日期:2009-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1023/a:1015500431421
更新日期:2002-08-01 00:00:00
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
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更新日期:2003-09-01 00:00:00
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更新日期:2007-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2016-06-01 00:00:00
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