Unusual sequence of an abscisic acid-inducible mRNA which accumulates late in Brassica napus seed development.

abstract：:A clone encoding a proline-rich protein (ZmPRP) has been obtained from maize root by differential screening of a maturing elongation root cDNA library. The amino acid sequence deduced from the full-length cDNA contains a putative signal peptide and a highly repetitive sequence containing the PEPK motif, indicating tha...

journal_title：Plant molecular biology

authors： Vignols F,José-Estanyol M,Caparrós-Ruiz D,Rigau J,Puigdomènech P

更新日期：1999-03-01 00:00:00

abstract：:During maize seed development, endosperm cells synthesize large amounts of storage proteins, alpha-, beta-, and gamma-zeins, which accumulate within endoplasmic reticulum (ER)-derived protein bodies. The absence of lysine in all zein polypeptides results in an imbalance in the amino acid composition of maize seeds. We...

journal_title：Plant molecular biology

authors： Torrent M,Alvarez I,Geli MI,Dalcol I,Ludevid D

更新日期：1997-05-01 00:00:00

abstract：KEY MESSAGE:Lack of structural components results in inflorescence stem bending. Differentially expressed genes involved in lignin and hemicellulose biosynthesis are vital; genes involved in cellulose and glycan biosynthesis are also relevant. An erect inflorescence stem is essential for high-quality cut herbaceous peo...

journal_title：Plant molecular biology

authors： Wan Y,Zhang M,Hong A,Lan X,Yang H,Liu Y

更新日期：2020-02-01 00:00:00

abstract：:A chimeric gene consisting of the 5' flanking sequences of a rice glutelin gene (Gt3) linked to the chloramphenicol acetyltransferase (CAT) coding segment was introduced into tobacco via Agrobacterium tumefaciens-mediated transformation. CAT enzyme activity could be detected in extracts from seeds as early as 8 days a...

journal_title：Plant molecular biology

authors： Leisy DJ,Hnilo J,Zhao Y,Okita TW

更新日期：1990-01-01 00:00:00

abstract：:A hybrid Cauliflower Mosaic Virus (CaMV) genome containing a selectable marker gene was constructed by replacing the gene VI coding region with the aminoglycoside (neomycin) phosphotransferase type II [APH(3')II] gene from Tn5. This modified viral genome was tested for its infectivity both in planta and in a protoplas...

journal_title：Plant molecular biology

authors： Paszkowski J,Pisan B,Shillito RD,Hohn T,Hohn B,Potrykus I

更新日期：1986-09-01 00:00:00

abstract：:In the wake of recent progress of high throughput transcriptome profiling technologies, extensive housekeeping gene mining has been conducted in humans. However, very few studies have been reported in maize (Zea mays L.), an important crop plant, and none were conducted on a genome -wide level. In this study, we surve...

journal_title：Plant molecular biology

authors： Lin F,Jiang L,Liu Y,Lv Y,Dai H,Zhao H

更新日期：2014-11-01 00:00:00

abstract：:Using a yeast two-hybrid system, we identified a plant cellular factor that interacts with the proteins of the Cucumber mosaic virus (CMV). Initially 14 candidate genes were isolated from Nicotiana tabacum, using a full-length CMV 1a gene as bait. Among the candidate genes, two were encoding thaumatin-like proteins (T...

journal_title：Plant molecular biology

authors： Kim MJ,Ham BK,Kim HR,Lee IJ,Kim YJ,Ryu KH,Park YI,Paek KH

更新日期：2005-12-01 00:00:00

abstract：:The PR-like proteins, class I beta-1,3-glucanase (GLU I) and chitinase (CHN I), are induced as part of a stereotypic response that can provide protection against viral, bacterial, and fungal pathogens. We have identified two Nicotiana plumbaginifolia ankyrin-repeat proteins, designated Glucanohydrolase Binding Protein...

journal_title：Plant molecular biology

authors： Wirdnam C,Motoyama A,Arn-Bouldoires E,van Eeden S,Iglesias A,Meins F Jr

更新日期：2004-11-01 00:00:00

abstract：:Electrophoretic analysis of translation products of polyadenylated RNA isolated from mid-maturation sorghum seed in the presence of [(35)S]met, [(3)H]leu, or [(3)H]val revealed two major proteins of kDa and 21 kDa. These products were not detected when [(3)H]lys was supplied as the radioactive substrate. Under similar...

journal_title：Plant molecular biology

authors： Derose RT,Ma DP,Kwon IS,Hasnain SE,Klassy RC,Hall TC

更新日期：1989-03-01 00:00:00

abstract：KEY MESSAGE:In this manuscript, we disclosed the influence of light on the accumulation of storage reserves in B. napus embryos.1.Light induced the gene expression in the developing embryos of B. napus.2.Light promoted the starch synthesis in chloroplasts of B. napus embryos.3.Light enhanced the metabolic activity of s...

journal_title：Plant molecular biology

authors： Tan H,Qi X,Li Y,Wang X,Zhou J,Liu X,Shi X,Ye W,Xiang X

更新日期：2020-07-01 00:00:00

abstract：:Plant infections with cassava latent virus (CLV) were mediated by the Ti plasmid of Agrobacterium tumefaciens containing either monomeric or dimeric copies of the virus genome. The CLV DNAs caused typical symptoms when they were inoculated in Agrobacterium strains C58, LBA4404 and a virE mutant A1026, but not other Ag...

journal_title：Plant molecular biology

authors： Morris BA,Richardson KA,Andersen MT,Gardner RC

更新日期：1988-11-01 00:00:00

abstract：:We have identified two single-copy genes from the model legume. Medicago truncatula (MtENOD16 and 20) whose expression can be correlated with early stages of root nodulation and whose predicted coding sequences are partially homologous to both pea/vetch ENOD5 and soybean N315/ENOD55. Database searching and sequence al...

journal_title：Plant molecular biology

authors： Greene EA,Erard M,Dedieu A,Barker DG

更新日期：1998-03-01 00:00:00

abstract：:Despite the presence in nature of many functional gene families that contain several to many highly similar sequences, the presence of identical DNA sequence repeats is widely thought to predispose transgene inserts to homology dependent gene silencing (HDGS). The induction of transcriptional gene silencing (TGS) by R...

journal_title：Plant molecular biology

authors： Yang G,Lee YH,Jiang Y,Kumpatla SP,Hall TC

更新日期：2005-06-01 00:00:00

abstract：:Two types of structurally distinct O-methyltransferases mediate the methylation of hydroxylated monomeric lignin precursors in angiosperms. Caffeate 3-O-methyltransferase (COMT; EC 2.1.1.68) methylates the free acids and caffeoyl CoA 3-O-methyltransferase (CCoAOMT; EC 2.1.1.104) methylates coenzyme A esters. Recently,...

journal_title：Plant molecular biology

authors： Li L,Osakabe Y,Joshi CP,Chiang VL

更新日期：1999-07-01 00:00:00

abstract：:A 7.4 kb segment of a Euglena nuclear gene encoding a portion of the polyprotein precursor to the light-harvesting chlorophyll a/b-binding protein of photosystem II (LHCPII) has been isolated and sequenced. Nine exons can be assembled into a continuous open reading frame encoding 113 amino acids of the C-terminus of a...

journal_title：Plant molecular biology

authors： Muchhal US,Schwartzbach SD

更新日期：1992-01-01 00:00:00

abstract：:The chromatin structure of pea (Pisum sativum) rbcS genes in inactive (root), potentially active (dark-grown leaf), and active states (light-grown leaf) was analysed using (a) pancreatic DNAseI to detect general DNAseI sensitivity and DNAseI-hypersensitive sites, and (b) methyl-sensitive restriction endonucleases to p...

journal_title：Plant molecular biology

authors： Görz A,Schäfer W,Hirasawa E,Kahl G

更新日期：1988-09-01 00:00:00

abstract：:The phytohormone abscisic acid is probably present in all higher plants. This hormone is necessary for regulation of several events during seed development and for the response to environmental stresses such as desiccation, salt and cold. An important part of the physiological response to abscisic acid is achieved thr...

journal_title：Plant molecular biology

authors： Busk PK,Pagès M

更新日期：1998-06-01 00:00:00

abstract：:A cDNA clone encoding Arabidopsis thaliana galactokinase was fortuitously isolated during the course of a screen for plant homologues of a Saccharomyces cerevisiae peroxisome assembly gene, PAS9. Clones were sought which restored the ability of pas9 cells to grow on oleate as a sole carbon source, as oleate metabolism...

journal_title：Plant molecular biology

authors： Kaplan CP,Tugal HB,Baker A

更新日期：1997-06-01 00:00:00

abstract：:In biosynthesis of octadecanoids and jasmonate (JA), the naturally occurring enantiomer is established in a step catalysed by the gene cloned recently from tomato as a single-copy gene (Ziegler et al., 2000). Based on sequence homology, four full-length cDNAs were isolated from Arabidopsis thaliana ecotype Columbia co...

journal_title：Plant molecular biology

authors： Stenzel I,Hause B,Miersch O,Kurz T,Maucher H,Weichert H,Ziegler J,Feussner I,Wasternack C

更新日期：2003-04-01 00:00:00

abstract：:We isolated a rice cDNA encoding nucleoside diphosphate kinase (NDK, EC 2.7.4.6). The deduced amino acid sequence of the rice NDK shows highest homology to spinach NDK-I. The rice NDK gene exhibits a strong codon bias (73.8% GC) in the third position of the codon. DNA blot analysis indicated that at least single NDK g...

journal_title：Plant molecular biology

authors： Yano A,Shimazaki T,Kato A,Umeda M,Uchimiya H

更新日期：1993-12-01 00:00:00

abstract：:Acyl-ACP thioesterases are involved in regulating chain termination of fatty acid biosynthesis in plant systems. Previously, acyl-ACP thioesterase purified from Brassica napus seed tissue has been shown to have a high preference for hydrolysing oleoyl-ACP. Here, oligonucleotides derived from B. napus oleoyl-ACP thioes...

journal_title：Plant molecular biology

authors： Loader NM,Woolner EM,Hellyer A,Slabas AR,Safford R

更新日期：1993-11-01 00:00:00

abstract：:The ubiquitous grapevine-associated octopine/cucumopine Ti plasmids of biotype III Agrobacterium tumefaciens strains carry two T regions, TA and TB, with a complex oncogene arrangement. Within the octopine/cucumopine group, two main strain types were identified: 'large TA' strains with a TA region resembling the TL re...

journal_title：Plant molecular biology

authors： Huss B,Tinland B,Paulus F,Walter B,Otten L

更新日期：1990-02-01 00:00:00

abstract：:The microtubular element of the plant cytoskeleton undergoes dramatic architectural changes in the course of the cell cycle, specifically at the entry into and exit from mitosis. These changes underlie the acquisition of specialized properties and functions involved, for example, in the equal segregation of chromosome...

journal_title：Plant molecular biology

authors： Vantard M,Cowling R,Delichère C

更新日期：2000-08-01 00:00:00

abstract：:In this report, we describe the efficient expression and glycosylation, in insect cells, of β-phaseolin polypeptides (M r 45 and 48 kDa) from Phaseolus vulgaris, by means of a baculovirus expression vector. N-terminal sequence analysis demonstrated that the signal peptide was efficiently processed. Tunicamycin treatme...

journal_title：Plant molecular biology

authors： Bustos MM,Luckow VA,Griffing LR,Summers MD,Hall TC

更新日期：1988-11-01 00:00:00

abstract：KEY MESSAGE:CYP71Z18 exhibited plastic substrate specificity to catalyze oxidation of multiple rice diterpenes and elevated chemical defense against the blast fungus in transgenic rice. Diversified plant specialized metabolism relies on corresponding biosynthetic enzymes with differential substrate specificity. CYP71Z1...

journal_title：Plant molecular biology

authors： Shen Q,Pu Q,Liang J,Mao H,Liu J,Wang Q

更新日期：2019-08-01 00:00:00

abstract：:Proline-rich protein (PRP), a cell wall protein of plant, has been studied in many plant species. Yet, none of the PRPs has been functionally elucidated. Here we report a novel flower-specific PRP designated OsPRP3 from rice. Expression analysis showed that the OsPRP3 transcript was mainly present in rice flower and a...

journal_title：Plant molecular biology

authors： Gothandam KM,Nalini E,Karthikeyan S,Shin JS

更新日期：2010-01-01 00:00:00

abstract：:T-DNA tagging is a high throughput strategy for identifying and cloning functional genes in plants. In this study, we screened 4416 lab-created T(1) rice T-DNA tagged lines and identified a mutant, designated dh1 (degenerated hull1), with phenotype of degenerated hull and naked pistils and stamens. Approximately 60% f...

journal_title：Plant molecular biology

authors： Li A,Zhang Y,Wu X,Tang W,Wu R,Dai Z,Liu G,Zhang H,Wu C,Chen G,Pan X

更新日期：2008-03-01 00:00:00

abstract：:Controlled cellular suicide is an important process that can be observed in various organs during plant development. From the generation of proper sexual organs in monoecious plants to the hypersensitive response (HR) that occurs during incompatible pathogen interactions, programmed cell death (PCD) can be readily obs...

journal_title：Plant molecular biology

authors： Pontier D,Gan S,Amasino RM,Roby D,Lam E

更新日期：1999-04-01 00:00:00

abstract：:The presence of specific glycine-rich proteins (GRP) related to petunia GRP1 (ptGRP1) was examined in three species of monocots (wheat, barley and maize) and five species of dicots (rape, turnip, soybean, crabapple and tomato). Protein blot analysis showed that anti-ptGRP1 antibody cross-reacted with a single differen...

journal_title：Plant molecular biology

authors： Cheng SH,Keller B,Condit CM

更新日期：1996-04-01 00:00:00

abstract：:Antisera against the N-terminal and C-terminal parts of the potential ORF IV product were used to analyse extracts from CaMV-infected turnip leaves by immunoblotting. Polypeptides of 87, 83, 82, 60 and 57 kDa were detected. The origin of these proteins is discussed. ...

journal_title：Plant molecular biology

authors： Kirchherr D,Albrecht H,Mesnard JM,Lebeurier G

更新日期：1988-05-01 00:00:00