Abstract:
:Two S-adenosylmethionine synthetase (SAMS) cDNAs, PcSAMS1 and PcSAMS2, have been identified in Pinus contorta. We found that the two genes are differentially expressed during root development. Thus, PcSAMS1 is preferentially expressed in roots and exhibits a specific expression pattern in the meristem at the onset of adventitious root development, whereas PcSAMS2 is expressed in roots as well as in shoots and is down-regulated during adventitious root formation. The expression of the two SAMS genes is different from the SAMS activity levels during adventitious root formation. We conclude that other SAMS genes that remain to be characterized may contribute to the observed SAMS activity, or that the activities of PcSAMS1 and PcSAMS2 are affected by post-transcriptional regulation. The deduced amino acid sequences of PcSAMS1 and PcSAMS2 are highly divergent, suggesting different functional roles. However, both carry the two perfectly conserved motifs that are common to all plant SAMS. At the protein level, PcSAMS2 shares about 90% identity to other isolated eukaryotic SAMS, while PcSAMS1 shares less than 50% identity with other plant SAMS. In a phylogenetic comparison, PcSAMS1 seems to have diverged significantly from all other SAMS genes. Nevertheless, PcSAMS1 was able to complement a Saccharomyces cerevisiae sam1 sam2 double mutant, indicating that it encodes a functional SAMS enzyme.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Lindroth AM,Saarikoski P,Flygh G,Clapham D,Grönroos R,Thelander M,Ronne H,von Arnold Sdoi
10.1023/a:1010637012528subject
Has Abstractpub_date
2001-06-01 00:00:00pages
335-46issue
3eissn
0167-4412issn
1573-5028journal_volume
46pub_type
杂志文章abstract::Considerable progress in tomato molecular biology has been made over the past five years. At least 19 different mRNAs which increase in amount during tomato fruit ripening have been cloned and genes for enzymes involved in cell wall degradation (polygalacturonase and pectinesterase) and ethylene synthesis (ACC synthas...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/BF00015607
更新日期:1992-05-01 00:00:00
abstract::Dehydrins are characterized by the consensus KIKEKLPG amino acid sequence found near the carboxy terminus, and usually repeated from one to many times within the protein. A synthetic peptide containing this consensus sequence was used to produce specific antibodies that recognize dehydrins in a wide range of plants. T...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00029004
更新日期:1993-10-01 00:00:00
abstract::The synthesis of both strands of CaMV-DNA has been studied in vitro using viral replication complexes obtained by hypotonic extraction of infected plant organelles. Hybridization of the DNA synthesized in vitro to single stranded CaMV DNA probes cloned in bacteriophage M 13 confirmed that the 35 S RNA served as a temp...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017779
更新日期:1984-09-01 00:00:00
abstract::A method (termed co-cultivation) for transforming plant cells in vitro with A. tumefaciens strains, which was originally developed by Marton et al. (1978) Nature 277: 129-131, has been modified by the incorporation of a novel feeder plate culture system and been extended to use with petunia protoplasts. Using efficien...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00033384
更新日期:1984-11-01 00:00:00
abstract::Plants perceiving drought activate multiple responses to improve survival, including large-scale alterations in gene expression. This article reports on the roles in the drought response of two Arabidopsis thaliana homeodomain-leucine zipper class I genes; ATHB7 and ATHB12, both strongly induced by water-deficit and a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-012-9956-4
更新日期:2012-11-01 00:00:00
abstract::A genomic library was prepared from Populus kitakamiensis and screened with the cDNA for an anionic peroxidase from P. kitakamiensis. One genomic clone was isolated that contained two tandemly oriented genes for anionic peroxidases, prxA3a and prxA4a. Both genes consisted of four exons and three introns; the introns h...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00021193
更新日期:1995-07-01 00:00:00
abstract::As an important osmoprotectant, glycine betaine (GB) plays an essential role in resistance to abiotic stress in a variety of organisms, including rice (Oryza sativa L.). However, GB content is too low to be detectable in rice, although rice genome possesses several orthologs coding for betaine aldehyde dehydrogenase (...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0239-0
更新日期:2014-11-01 00:00:00
abstract::In higher eudicotyledonous angiosperms the floral organs are typically arranged in four different whorls, containing sepals, petals, stamens and carpels. According to the ABC model, the identity of these organs is specified by floral homeotic genes of class A, A+B, B+C and C, respectively. In contrast to the sepal and...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1025070827979
更新日期:2003-07-01 00:00:00
abstract::An 8 Kilobase-pair (Kbp) HindIII fragment containing the coding sequence forSpirulina platensis glutamine synthetase [EC 6.3.1.1.] has been identified utilizing a probe derived fromAnabaena 7120 and cloned in the vector pAT153. ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF02418760
更新日期:1985-03-01 00:00:00
abstract::A gene family of at least five members encodes the tobacco mitochondrial Rieske Fe-S protein (RISP). To determine whether all five RISPs are translocated to mitochondria, fusion proteins containing the putative presequences of tobacco RISPs and Escherichia coli beta-glucuronidase (GUS) were expressed in transgenic tob...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020982
更新日期:1995-11-01 00:00:00
abstract::We have constructed a yeast artificial chromosome (YAC) library using high-molecular-weight DNA prepared from agarose-embedded leaf protoplasts of the maize inbred line UE95. This library contains 79,000 clones with an average insert size of 145 kb and should therefore represent approximately three haploid genome equi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027351
更新日期:1992-05-01 00:00:00
abstract::Plants that utilize the highly efficient C4 photosynthetic pathway possess two types of specialized leaf cells, the mesophyll and bundle sheath. In mature leaves of amaranth, a dicotyledonous C4 plant, ribulose 1,5-bisphosphate carboxylase (Rubisco) is localized specifically to the chloroplasts of bundle sheath cells,...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015971
更新日期:1993-06-01 00:00:00
abstract::The caffeine biosynthetic pathway in coffee plants has been proposed to involve three distinct N -methyltransferases, xanthosine methyltransferase (XMT), 7- N -methylxanthine methyltransferase (MXMT; theobromine synthase), and 3,7-dimethylxanthine methyltransferase (DXMT; caffeine synthase). We previously isolated all...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-0393-x
更新日期:2004-04-01 00:00:00
abstract::Peach fruits subjected to prolonged cold storage (CS) to delay decay and over-ripening often develop a form of chilling injury (CI) called mealiness/woolliness (WLT), a flesh textural disorder characterized by lack of juiciness. Transcript profiles were analyzed after different lengths of CS and subsequent shelf life ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0526-z
更新日期:2016-11-01 00:00:00
abstract::A cold-regulated gene (cor tmc-ap3) coding for a putative chloroplastic amino acid selective channel protein was isolated from cold-treated barley leaves combining the differential display and the 5'-RACE techniques. Cor tmc-ap3 is expressed at low level under normal growing temperature, and its expression is strongly...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006375332677
更新日期:1999-09-01 00:00:00
abstract::We report the isolation of the second member, kin2, of a family of two cold-inducible genes of Arabidopsis thaliana. The proteins corresponding to the two genes have similarities to the small antifreeze proteins from Winter flounder. Kin1 and kin2 are organized in a close tandem array in the genome of A. thaliana. Bot...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00026794
更新日期:1992-07-01 00:00:00
abstract::A chimeric gene consisting of a bean (Phaseolus vulgaris L.) chalcone synthase (CHS) promoter fused to a bacterial chloramphenicol acetyltransferase (CAT) reporter gene was strongly expressed, and further induced by fungal elicitor, when electroporated into alfalfa (Medicago sativa L.) suspension cell protoplasts. Fun...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015079
更新日期:1991-05-01 00:00:00
abstract::We have previously shown that the expression of SmCP which encodes Solanum melongena cysteine proteinase is ethylene-inducible and is under circadian control. To understand the regulation of SmCP, a 1.34-kb SmCP 5'-flanking region and its deletion derivatives were analyzed for cis-elements using GUS and luc fusions an...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-0954-7
更新日期:2005-03-01 00:00:00
abstract::We have isolated cDNA clones encoding the pentose phosphate pathway enzymes 6-phosphogluconate dehydrogenase (6PGDH, EC 1.1.1.44) and glucose 6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) from alfalfa (Medicago sativa L.). These exhibit extensive nucleotide and amino acid sequence similarity to the corresponding gene...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00042073
更新日期:1995-08-01 00:00:00
abstract::Synthetic oligonucleotides corresponding to conserved regions of cysteine proteinases were used as primers in the RT-PCR amplification of a fragment of cDNA corresponding to a region of a cysteine proteinase gene expressed during germination of chickpea (cac for Cicer arietinum cysteine proteinase). The identity of th...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023238
更新日期:1994-05-01 00:00:00
abstract::The amounts of a 1.9 kb mitochondrial plasmid relative to sequences in another mitochondrial DNA replicon and also to nuclear ribosomal DNA sequences have been compared in maize leaves and anthers. Similar comparisons have been made between plants with the same nuclear genotype but containing normal, S, or T cytoplasm...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF02418241
更新日期:1985-07-01 00:00:00
abstract::We present evidence for a unique covalent modification of a nuclear-encoded precursor protein targeted to plant mitochondria. We investigated the early events of in vitro import for the mitochondrial precursor of the ATP synthase F1beta subunit from Nicotiana plumbaginifolia (pF1beta) into plant mitochondria. When pF1...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006375123496
更新日期:1999-11-01 00:00:00
abstract:KEY MESSAGE:IEF, a novel plasma plasma membrane protein, is important for exine formation in Arabidopsis. Exine, an important part of pollen wall, is crucial for male fertility. The major component of exine is sporopollenin which are synthesized and secreted by tapetum. Although sporopollenin synthesis has been well st...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01114-8
更新日期:2021-01-22 00:00:00
abstract::Salicylic acid (SA) is known to play an important role in the interaction between plant and micro-organisms, both symbiotic and pathogen. In particular, high levels of SA block nodule formation and mycorrhizal colonization in plants. A mutant of Lotus japonicus, named Ljsym4-2, was characterized as unable to establish...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-009-9585-8
更新日期:2010-03-01 00:00:00
abstract::The histidine auxotroph mutant his 1(-) isolated from Nicotiana plumbaginifolia haploid protoplasts was first characterized to be deficient for the enzyme histidinol phosphate aminotransferase that is responsible for one of the last steps of histidine biosynthesis. Expression of the mutated gene at the RNA level was a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006493021557
更新日期:2001-01-01 00:00:00
abstract::Nodules of tropical legumes generally export symbiotically fixed nitrogen in the form of ureides that are produced by oxidation of de novo synthesized purines. To investigate the regulation of de novo purine biosynthesis in these nodules, we have isolated cDNA clones encoding 5-aminoimidazole ribonucleotide (AIR) carb...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020176
更新日期:1994-01-01 00:00:00
abstract::An approximately 247-kb genomic region from FF genome of wild rice Oryza brachyantha, possessing the smallest Oryza genome, was compared to the orthologous approximately 450-kb region from AA genome, O. sativa L. ssp. japonica. 37 of 38 genes in the orthologous regions are shared between japonica and O. brachyantha. A...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9178-3
更新日期:2007-07-01 00:00:00
abstract::The soybean leghemoglobin lba gene promoter sequence was determined and aligned with the promoter sequence of the soybean lbc3 gene from the same gene family. Five highly conserved regions were found. There are two large conserved regions, one of which overlaps the basic promoter while the other defines a minimal enha...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027380
更新日期:1993-08-01 00:00:00
abstract::We have isolated and characterized the genomic clone of maize casein kinase 2 (CK2) alpha subunit using the previously described alphaCK2-1 cDNA clone as a probe. The genomic clone is 7.5 kb long and contains 10 exons, separated by 9 introns of different size, two larger than 1.5 kb and the others around 100-150 bp. T...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006196530079
更新日期:1999-05-01 00:00:00
abstract::Bacteroids of Rhizobium leguminosarum in root nodules of Pisum sativum are enclosed by a plant-derived peribacteriod membrane (PBM). The contents of the interstitial peribacteroid space (PBS) between bacteroid membrane and PBM were isolated by a controlled osmotic shock of PBM-enclosed bacteroids and analysed by two-d...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015670
更新日期:1988-03-01 00:00:00