A view of plant dehydrins using antibodies specific to the carboxy terminal peptide.

abstract：:Immunofluorescence flow cytometry was used to study the distribution of viral antigen in protoplast populations. Protoplasts were isolated from healthy and alfalfa mosaic virus (AMV) infected tobacco leaves (designated in vivo infected). Furthermore isolated tobacco and cowpea protoplasts were infected in vitro with A...

journal_title：Plant molecular biology

authors： van Klaveren P,Slats J,Roosien J,van Vloten-Doting L

更新日期：1983-01-01 00:00:00

abstract：:MYB transcription factors are a large family of proteins involved in the regulation of secondary metabolism and cell shape, the enhancement of disease resistance and the response to different stresses. In this study, the role of TaMYB4 in wheat against biotic and abiotic stresses was investigated. TaMYB4 was cloned fr...

journal_title：Plant molecular biology

authors： Al-Attala MN,Wang X,Abou-Attia MA,Duan X,Kang Z

更新日期：2014-03-01 00:00:00

abstract：:GALACTINOL SYNTHASE is the first committed enzyme in the raffinose biosynthetic pathway. We have previously characterized the maize (Zea mays) GALACTINOL SYNTHASE2 gene (ZmGOLS2) as abiotic stress induced. To further investigate the regulation of ZmGOLS2 gene expression, individual luciferase expression vectors,in whi...

journal_title：Plant molecular biology

authors： Gu L,Zhang Y,Zhang M,Li T,Dirk LM,Downie B,Zhao T

更新日期：2016-01-01 00:00:00

abstract：:Agrobacterium-mediated gene transfer is the method of choice for many plant biotechnology laboratories; however, large-scale use of this organism in conifer transformation has been limited by difficult propagation of explant material, selection efficiencies and low transformation frequency. We have analyzed co-cultiva...

journal_title：Plant molecular biology

authors： Wenck AR,Quinn M,Whetten RW,Pullman G,Sederoff R

更新日期：1999-02-01 00:00:00

abstract：:A cDNA library from ethephon-treated cucumber cotyledons (Cucumis sativus L. cv. Poinsett 76) was constructed. Two cDNA clones encoding putative peroxidases were isolated by means of a synthetic probe based on a partial amino acid sequence of a 33 kDa cationic peroxidase that had been previously shown to be induced by...

journal_title：Plant molecular biology

authors： Morgens PH,Callahan AM,Dunn LJ,Abeles FB

更新日期：1990-05-01 00:00:00

abstract：:Beyond the rewards of plant genome analysis and gene identification, characterisation of protein activities, post-translational modifications and protein complex composition remains a challenge for plant biologists. Ideally, methods should allow rapid isolation of proteins from plant material achieving a high degree o...

journal_title：Plant molecular biology

authors： Witte CP,Noël LD,Gielbert J,Parker JE,Romeis T

更新日期：2004-05-01 00:00:00

abstract：:The putative RNA helicase encoded by the Arabidopsis gene At1g32490 is a homolog of the yeast splicing RNA helicases Prp2 and Prp22. We isolated a temperature-sensitive allele (rsw12) of the gene in a screen for root radial swelling mutants. Plants containing this allele grown at the restrictive temperature showed wea...

journal_title：Plant molecular biology

authors： Howles PA,Gebbie LK,Collings DA,Varsani A,Broad RC,Ohms S,Birch RJ,Cork AH,Arioli T,Williamson RE

更新日期：2016-05-01 00:00:00

abstract：:As an important osmoprotectant, glycine betaine (GB) plays an essential role in resistance to abiotic stress in a variety of organisms, including rice (Oryza sativa L.). However, GB content is too low to be detectable in rice, although rice genome possesses several orthologs coding for betaine aldehyde dehydrogenase (...

journal_title：Plant molecular biology

authors： Tang W,Sun J,Liu J,Liu F,Yan J,Gou X,Lu BR,Liu Y

更新日期：2014-11-01 00:00:00

abstract：:Porphobilinogen deaminase (PBG deaminase) is an early enzyme of the pathway for chlorophyll and heme synthesis. Using degenerate oligonucleotide primers, based on amino acid sequence data for purified PBG deaminase from pea, a fragment was amplified from Arabidopsis genomic DNA by PCR, and then used to isolate both a ...

journal_title：Plant molecular biology

authors： Lim SH,Witty M,Wallace-Cook AD,Ilag LI,Smith AG

更新日期：1994-11-01 00:00:00

abstract：:Phaseolin is a glycoprotein that constitutes the major storage protein in bean seeds. The phaseolin gene promoters function in a seed-specific manner. In an attempt to understand if events following transcription of the gene also contribute to the seed-specific accumulation of the phaseolin protein, we studied the eff...

journal_title：Plant molecular biology

authors： Bagga S,Sutton D,Kemp JD,Sengupta-Gopalan C

更新日期：1992-09-01 00:00:00

abstract：KEY MESSAGE:TaPSK5 is a less conserved target of miR164 in wheat encoding a positive regulator of root growth and yield traits that could be used for crop improvement. MicroRNAs (miRNAs) play key roles in regulating plant growth and development by targeting the mRNAs of conserved genes. However, little is known about t...

journal_title：Plant molecular biology

authors： Geng Y,Jian C,Xu W,Liu H,Hao C,Hou J,Liu H,Zhang X,Li T

更新日期：2020-12-01 00:00:00

abstract：:The expression of the alpha' and beta subunit genes of beta-conglycinin is differentially regulated during soybean embryo development. Although both are expressed solely in developing seeds during mid to late stages of embryo development, the alpha' subunit is expressed more highly on a per gene basis, and alpha' subu...

journal_title：Plant molecular biology

authors： Lessard PA,Allen RD,Bernier F,Crispino JD,Fujiwara T,Beachy RN

更新日期：1991-03-01 00:00:00

abstract：:The synthesis of both strands of CaMV-DNA has been studied in vitro using viral replication complexes obtained by hypotonic extraction of infected plant organelles. Hybridization of the DNA synthesized in vitro to single stranded CaMV DNA probes cloned in bacteriophage M 13 confirmed that the 35 S RNA served as a temp...

journal_title：Plant molecular biology

authors： Pfeiffer P,Laquel P,Hohn T

更新日期：1984-09-01 00:00:00

abstract：:Glucosinolates are a class of thioglycosides found predominantly in plants of the order Brassicales whose function in anti-herbivore defense has been attributed to the products formed by myrosinase-catalyzed hydrolysis upon plant tissue damage. The most common type of hydrolysis products, the isothiocyanates, are toxi...

journal_title：Plant molecular biology

authors： Burow M,Bergner A,Gershenzon J,Wittstock U

更新日期：2007-01-01 00:00:00

abstract：:We have cloned and sequenced a gene, pds, from the cyanobacterium Synechococcus PCC7942 that is responsible for resistance to the bleaching herbicide norflurazon. A point mutation in that gene, leading to an amino acid substitution from valine to glycine in its polypeptide product, was found to confer this resistance....

journal_title：Plant molecular biology

authors： Chamovitz D,Pecker I,Hirschberg J

更新日期：1991-06-01 00:00:00

abstract：:Deepwater rice can grow in the regions of Southeast Asia that are flooded during the monsoon season because it has several adaptations allowing it to survive under flooded conditions. One such adaptation is the ability for rapid internode elongation upon partial submergence to maintain its foliage above the rising flo...

journal_title：Plant molecular biology

authors： Zarembinski TI,Theologis A

更新日期：1997-01-01 00:00:00

abstract：:Soil contamination by hexavalent chromium [Cr(VI) or chromate] due to anthropogenic activities has become an increasingly important environmental problem. To date few studies have been performed to elucidate the signaling networks involved on adaptive responses to (CrVI) toxicity in plants. In this work, we report tha...

journal_title：Plant molecular biology

authors： Martínez-Trujillo M,Méndez-Bravo A,Ortiz-Castro R,Hernández-Madrigal F,Ibarra-Laclette E,Ruiz-Herrera LF,Long TA,Cervantes C,Herrera-Estrella L,López-Bucio J

更新日期：2014-09-01 00:00:00

abstract：:The relative amounts of some chloroplast polypeptides were followed during greening of leaves from three different plant families. Oat, bean and spinach were the representatives of the Gramineae, Leguminosae and Chenopodiaceae, respectively. By using specific antibodies against subunits of the chloroplast protein comp...

journal_title：Plant molecular biology

authors： Nechushtai R,Nelson N

更新日期：1985-11-01 00:00:00

abstract：:Differentiation of the Arabidopsis thaliana (Arabidopsis) seed coat epidermal cells involves pronounced changes highlighted by the synthesis and secretion of copious amounts of dispensable, pectinaceous mucilage followed by a thick cellulosic secondary cell wall. This cell type, therefore, represents an excellent mole...

journal_title：Plant molecular biology

authors： Esfandiari E,Jin Z,Abdeen A,Griffiths JS,Western TL,Haughn GW

更新日期：2013-01-01 00:00:00

abstract：:The chloroplast gene for the epsilon subunit (atpE) of the CF1/CF0 ATPase in the green alga Chlamydomonas reinhardtii has been localized and sequenced. In contrast to higher plants, the atpE gene does not lie at the 3' end of the beta subunit (atpB) gene in the chloroplast genome of C. reinhardtii, but is located at a...

journal_title：Plant molecular biology

authors： Woessner JP,Gillham NW,Boynton JE

更新日期：1987-03-01 00:00:00

abstract：:Despite the central importance of the TCA cycle in plant metabolism not all of the genes encoding its constituent enzymes have been functionally identified. In yeast, the heterodimeric protein succinyl CoA ligase is encoded for by two single-copy genes. Here we report the isolation of two tomato cDNAs coding for alpha...

journal_title：Plant molecular biology

authors： Studart-Guimarães C,Gibon Y,Frankel N,Wood CC,Zanor MI,Fernie AR,Carrari F

更新日期：2005-11-01 00:00:00

abstract：:A 834 bp cDNA encoding calmodulin (CaM) has been isolated from Brassica juncea. On Northern analysis this cDNA hybridises this cDNA to mRNAs of about 0.9 kb in leaf, silique and peduncle. Genomic Southern analysis indicates the presence of a CaM multigene family in Brassica juncea. Comparison of the predicted amino ac...

journal_title：Plant molecular biology

authors： Chye ML,Liu CM,Tan CT

更新日期：1995-01-01 00:00:00

abstract：:To isolate differentially expressed genes during the juvenile-to-adult phase transition of an early-flowering trifoliate orange mutant (precocious trifoliate orange, Poncirus trifoliata), suppression subtractive hybridization was performed. In total, 463 cDNA clones chosen by differential screening of 1,920 clones wer...

journal_title：Plant molecular biology

authors： Zhang JZ,Ai XY,Sun LM,Zhang DL,Guo WW,Deng XX,Hu CG

更新日期：2011-05-01 00:00:00

abstract：:TGA1a is a well-characterized transcription factor that may mediate the root-specific and auxin-responsive expression of some plant genes. In tobacco, Southern blot and genomic cloning analyses have shown that TGA1a consists of at least four closely related genes. Since TGA1a belongs to the bZIP class of transcription...

journal_title：Plant molecular biology

authors： Miao ZH,Liu X,Lam E

更新日期：1994-04-01 00:00:00

abstract：KEY MESSAGE:MdMYB16 forms homodimers and directly inhibits anthocyanin synthesis via its C-terminal EAR repressor. It weakened the inhibitory effect of MdMYB16 on anthocyanin synthesis when overexpressing MdbHLH33 in callus overexpressing MdMYB16. MdMYB16 could interact with MdbHLH33. Anthocyanins are strong antioxidan...

journal_title：Plant molecular biology

authors： Xu H,Wang N,Liu J,Qu C,Wang Y,Jiang S,Lu N,Wang D,Zhang Z,Chen X

更新日期：2017-05-01 00:00:00

abstract：:Heat shock proteins (hsps) are synthesized at higher temperatures in soybean seedlings exposed to a gradual temperature increase as compared to a rapid heat shock (hs). There are differences in the pattern of synthesis of different hsps depending on whether the temperature exposure is rapid or gradual. The synthesis o...

journal_title：Plant molecular biology

authors： Altschuler M,Mascarenhas JP

更新日期：1985-09-01 00:00:00

abstract：:During the course of characterizing polymerase chain reaction products corresponding to protein kinases of a higher plant, Arabidopsis thaliana, we found a DNA fragment that potentially codes for a polypeptide with mosaic sequences of two classes of protein kinases, a tyrosine-specific and a serine/threonine-specific ...

journal_title：Plant molecular biology

authors： Hirayama T,Oka A

更新日期：1992-11-01 00:00:00

abstract：:The PR-like proteins, class I beta-1,3-glucanase (GLU I) and chitinase (CHN I), are induced as part of a stereotypic response that can provide protection against viral, bacterial, and fungal pathogens. We have identified two Nicotiana plumbaginifolia ankyrin-repeat proteins, designated Glucanohydrolase Binding Protein...

journal_title：Plant molecular biology

authors： Wirdnam C,Motoyama A,Arn-Bouldoires E,van Eeden S,Iglesias A,Meins F Jr

更新日期：2004-11-01 00:00:00

abstract：:A MADS-box gene was isolated using the suppressive subtractive hybridization library between early-flowering mutant and wild-type trifoliate orange (Poncirus trifoliata L. Raf.). This gene is highly homologous with Arabidopsis SHORT VEGETATIVE PHASE (SVP). Based on real-time PCR and in situ hybridization during bud di...

journal_title：Plant molecular biology

authors： Li ZM,Zhang JZ,Mei L,Deng XX,Hu CG,Yao JL

更新日期：2010-09-01 00:00:00

abstract：:We have isolated and analysed a 2 kb region of the mitochondrial genome of Arabidopsis thaliana (Columbia) showing a high level of nucleotide identity with the mitochondrial (mt) rps14 small-subunit ribosomal protein gene from Oenothera berteriana and Vicia faba, as well as with an open reading frame (ORF) located ups...

journal_title：Plant molecular biology

authors： Aubert D,Bisanz-Seyer C,Herzog M

更新日期：1992-12-01 00:00:00