Abstract:
:Soil contamination by hexavalent chromium [Cr(VI) or chromate] due to anthropogenic activities has become an increasingly important environmental problem. To date few studies have been performed to elucidate the signaling networks involved on adaptive responses to (CrVI) toxicity in plants. In this work, we report that depending upon its concentration, Cr(VI) alters in different ways the architecture of the root system in Arabidopsis thaliana seedlings. Low concentrations of Cr (20-40 µM) promoted primary root growth, while concentrations higher than 60 µM Cr repressed growth and increased formation of root hairs, lateral root primordia and adventitious roots. We analyzed global gene expression changes in seedlings grown in media supplied with 20 or 140 µM Cr. The level of 731 transcripts was significantly modified in response to Cr treatment with only five genes common to both Cr concentrations. Interestingly, 23 genes related to iron (Fe) acquisition were up-regulated including IRT1, YSL2, FRO5, BHLH100, BHLH101 and BHLH039 and the master controllers of Fe deficiency responses PYE and BTS were specifically activated in pericycle cells. It was also found that increasing concentration of Cr in the plant correlated with a decrease in Fe content, but increased both acidification of the rhizosphere and activity of the ferric chelate reductase. Supply of Fe to Cr-treated Arabidopsis allowed primary root to resume growth and alleviated toxicity symptoms, indicating that Fe nutrition is a major target of Cr stress in plants. Our results show that low Cr levels are beneficial to plants and that toxic Cr concentrations activate a low-Fe rescue system.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Martínez-Trujillo M,Méndez-Bravo A,Ortiz-Castro R,Hernández-Madrigal F,Ibarra-Laclette E,Ruiz-Herrera LF,Long TA,Cervantes C,Herrera-Estrella L,López-Bucio Jdoi
10.1007/s11103-014-0210-0subject
Has Abstractpub_date
2014-09-01 00:00:00pages
35-50issue
1-2eissn
0167-4412issn
1573-5028journal_volume
86pub_type
杂志文章abstract::The actin gene superfamily of Petunia hybrida cv. Mitchell contains greater than 100 gene members which have been divided into several highly divergent subfamilies [1]. Five subfamily-specific probes have been used to compare the actin genes among the Mitchell, Violet 23 (V23) and Red 51 (R51) cultivars of P. hybrida....
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017466
更新日期:1988-09-01 00:00:00
abstract::DORNRÖSCHEN (DRN) and DORNRÖSCHEN-LIKE (DRNL) encode AP2-domain transcription factors, which act redundantly in cotyledon organogenesis. A more detailed genetic study now integrates DRN and DRNL into the CUP-SHAPED COTYLEDON (CUC) regulatory network and places DRN and DRNL differentially within the auxin signalling ne...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9721-5
更新日期:2011-02-01 00:00:00
abstract::The chloroplast psbB operon includes five genes encoding photosystem II and cytochrome b 6 /f complex components. The psbN gene is located on the opposite strand. PsbN is localized in the thylakoid and is present even in the dark, although its level increases upon illumination and then decreases. However, the translat...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0248-z
更新日期:2014-12-01 00:00:00
abstract::The expression of beta-amylase genes in rice (Oryza sativa) and its regulation by phytohormones gibberellic acid (GA) and abscisic acid (ABA) were examined. Upon germination beta-amylase is synthesized de novo in aleurone cells and (GA) is not required. Exogenous addition of GA does not enhance the beta-amylase activi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040716
更新日期:1996-08-01 00:00:00
abstract::We have previously shown that two CRE elements situated on a 31 bp region of the cauliflower mosaic virus (CaMV) 35S promoter activate gene expression in the yeast Saccharomyces cerevisiae and are regulated by cAMP. Studies with the yeast transcription factors GCN4, SKO1 and YAP1, which bind CRE-like sequences, showed...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023248
更新日期:1994-05-01 00:00:00
abstract::A 47 kDa glycoprotein, termed EP4, was purified from carrot cell suspension culture medium. An antiserum raised against EP4 also recognized a protein of 45 kDa that was ionically bound to the cell wall. EP4 was detected in culture media from both embryogenic and non-embryogenic cell lines and was found to be secreted ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00037018
更新日期:1995-03-01 00:00:00
abstract::Two dimensional gel electrophoresis was used to examine differences in steady state total poly(A) RNA from untransformed potato (Solanum tuberosum cv. Maris Bard) and potato transformed with shoot-inducing TL-DNA from A. tumefaciens. RNA was compared from phenotypically very distinct in vitro cultured shoots, more sim...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015227
更新日期:1986-07-01 00:00:00
abstract::Vascular tissue in plants is unique due to its diverse and dynamic cellular patterns. Signals controlling vascular development have only recently started to emerge through biochemical, genetic, and genomic approaches in several organisms, such as Arabidopsis, Populus, and Zinnia. These signals include hormones (auxin,...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-008-9374-9
更新日期:2009-03-01 00:00:00
abstract::MYB transcription factors are a large family of proteins involved in the regulation of secondary metabolism and cell shape, the enhancement of disease resistance and the response to different stresses. In this study, the role of TaMYB4 in wheat against biotic and abiotic stresses was investigated. TaMYB4 was cloned fr...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0156-7
更新日期:2014-03-01 00:00:00
abstract::A novel Rab GTPase protein in Arabidopsis thaliana, CPRabA5e (CP = chloroplast localized) is located in chloroplasts and has a role in transport. Transient expression of CPRabA5e:EGFP fusion protein in tobacco (Nicotiana tabacum) leaves, and immunoblotting using Arabidopsis showed localization of CPRabA5e in chloropla...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0161-x
更新日期:2014-04-01 00:00:00
abstract::The suppression of plant defence reactions plays a crucial role in causing plant diseases. In this report, we show that inducible plant defences are repressed during the development of Cercospora leaf spot disease. In the early phase of infection of sugar beet (Beta vulgaris L.) leaves with the phytopathogenic fungus ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-2141-7
更新日期:2004-08-01 00:00:00
abstract::Limonium bicolor, a typical recretohalophyte that lives in saline environments, excretes excessive salt to the environment through epidermal salt glands to avoid salt stress. The aim of this study was to screen for L. bicolor genes involved in salt secretion by high-throughput RNA sequencing. We established the experi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0460-0
更新日期:2016-06-01 00:00:00
abstract::Transformed root cultures of Nicotiana rustica have been generated in which the gene from the yeast Saccharomyces cerevisiae coding for ornithine decarboxylase has been integrated. The gene, driven by the powerful CaMV35S promoter with an upstream duplicated enhancer sequence, shows constitutive expression throughout ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017721
更新日期:1990-07-01 00:00:00
abstract::A 7.4 kb segment of a Euglena nuclear gene encoding a portion of the polyprotein precursor to the light-harvesting chlorophyll a/b-binding protein of photosystem II (LHCPII) has been isolated and sequenced. Nine exons can be assembled into a continuous open reading frame encoding 113 amino acids of the C-terminus of a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00034956
更新日期:1992-01-01 00:00:00
abstract::A number of A/T-rich sequences and a CATGCAT/A sequence are contained in the 5'-upstream regions of the genes encoding concanavalin A (Con A) and canavalin, two major seed storage proteins of Canavalia gladiata D.C. To study the role of these sequences in the seed-specific gene expression, we constructed 5'-deletion m...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020226
更新日期:1995-02-01 00:00:00
abstract::We have sequenced a plastid gene cluster from the unicellular red alga Cyanidium caldarium which is located downstream from the psbA gene and contains, in the following order, genes for a beta-allophycocyanin-like protein (apcB'), a putative 9.5 kDa allophycocyanin linker protein (apcL9.5) and a putative 29 kDa phycoc...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00014494
更新日期:1992-10-01 00:00:00
abstract::Plant cells respond to cold stress via a regulatory mechanism leading to enhanced cold acclimation accompanied by growth retardation. The C-repeat binding factor (CBF) signaling pathway is essential for cold response of flowering plants. Our previously study documented a novel CBF-like gene from the cold-tolerant Caps...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0181-1
更新日期:2014-06-01 00:00:00
abstract::In previous work, a 33 kDa cysteine proteinase was found in callus initiated from maize (Zea mays L.) resistant to fall armyworm feeding. A callus cDNA library from the maize inbred Mp708 was screened with oligonucleotides derived from the N-terminal amino acid sequence of the 33 kDa proteinase and several cDNA clones...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1026494813936
更新日期:1999-05-01 00:00:00
abstract::β-conglycinin, a major seed protein in soybean, is composed of α, α', and β subunits sharing a high homology among them. Despite its many health benefits, β-conglycinin has a lower amino acid score and lower functional gelling properties compared to glycinin, another major soybean seed protein. In addition, the α, α',...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9865-y
更新日期:2012-02-01 00:00:00
abstract::Peach fruits subjected to prolonged cold storage (CS) to delay decay and over-ripening often develop a form of chilling injury (CI) called mealiness/woolliness (WLT), a flesh textural disorder characterized by lack of juiciness. Transcript profiles were analyzed after different lengths of CS and subsequent shelf life ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0526-z
更新日期:2016-11-01 00:00:00
abstract::We have isolated cDNA clones encoding the pentose phosphate pathway enzymes 6-phosphogluconate dehydrogenase (6PGDH, EC 1.1.1.44) and glucose 6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) from alfalfa (Medicago sativa L.). These exhibit extensive nucleotide and amino acid sequence similarity to the corresponding gene...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00042073
更新日期:1995-08-01 00:00:00
abstract::DNA microsatellites are ubiquitously present in eukaryotic genomes and represent a vast source of highly informative markers. We describe in this article a (GGC)n microsatellite which is widely distributed in eukaryotic genomes. Using polymerase chain reaction (PCR) techniques and DNA sequencing, we demonstrated for t...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00014544
更新日期:1993-02-01 00:00:00
abstract::Cytosine methylation provides an attractive epigenetic modification for the global maintenance of phases in plant development; however, there are few known examples of specific genes whose methylation status changes in a developmentally regulated manner. Pl-Blotched, an allele of purple plant1 (pl1), which encodes a m...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-5620-6
更新日期:2006-03-01 00:00:00
abstract::Isolation of cDNAs encoding individual members of a gene family is essential for assessing their role in a biological phenomenon. However, this process is often laborious and slow due to highly conserved protein-coding region that interferes with the isolation of the individual members. Identification of gene-specific...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00042230
更新日期:1996-06-01 00:00:00
abstract::Chemically inducible gene switches can provide precise control over gene expression, enabling more specific analyses of gene function and expanding the plant biotechnology toolkit beyond traditional constitutive expression systems. The alc gene expression system is one of the most promising chemically inducible gene s...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0140-2
更新日期:2014-03-01 00:00:00
abstract::A combined chemical, biochemical and molecular study was conducted to understand the differential accumulation of volatile sesquiterpenes in melon fruits. Sesquiterpenes were present mainly in the rinds of climacteric varieties, and a great diversity in their composition was found among varieties. Sesquiterpenes were ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9296-6
更新日期:2008-04-01 00:00:00
abstract::A vector has been designed that contains a truncated CaMV (cauliflower mosaic virus) 35S promoter fused to a receptor gene encoding beta-glucuronidase (GUS), placed adjacent to the left border sequence of an Agrobacterium vector. In potato plants transformed with this vector, different patterns of transcription were d...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020557
更新日期:1991-02-01 00:00:00
abstract::Dehydrins are characterized by the consensus KIKEKLPG amino acid sequence found near the carboxy terminus, and usually repeated from one to many times within the protein. A synthetic peptide containing this consensus sequence was used to produce specific antibodies that recognize dehydrins in a wide range of plants. T...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00029004
更新日期:1993-10-01 00:00:00
abstract::We have isolated and characterized a cDNA from a strawberry fruit subtractive library that shows homology to class-I low-molecular-weight (LMW) heat-shock protein genes from other higher plants. The strawberry cDNA (clone njjs4) was a 779 bp full-length cDNA with a single open reading frame of 468 bp that is expected ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005994800671
更新日期:1998-01-01 00:00:00
abstract::We have identified by differential screening a novel Arabidopsis thaliana gene, called kin1, which is induced at +4 degrees C. The nucleotide sequences of both the genomic clone and the corresponding cDNA were determined. The deduced 6.5 kDa polypeptide has an unusual amino acid composition being rich in alanine, glyc...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017731
更新日期:1990-07-01 00:00:00