Phylogenetic distribution and genetic mapping of a (GGC)n microsatellite from rice (Oryza sativa L.).

Abstract:

:DNA microsatellites are ubiquitously present in eukaryotic genomes and represent a vast source of highly informative markers. We describe in this article a (GGC)n microsatellite which is widely distributed in eukaryotic genomes. Using polymerase chain reaction (PCR) techniques and DNA sequencing, we demonstrated for the first time in plant species that a (GGC)n microsatellite locus is moderately polymorphic. Six alleles are present at this locus in rice and length polymorphisms are caused by variation in the number of tandem GGC repeats. By scoring a backcross mapping population, we were able to demonstrate that this locus is stably inherited and does not link to any known RFLP markers on the rice RFLP map. Our results suggest that DNA microsatellites should be useful in plants for construction of genetic linkage maps, extension of the existing genetic linkage maps, linkage analysis of disease and pest resistance genes, and the study of population genetics.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Zhao X,Kochert G

doi

10.1007/BF00014544

subject

Has Abstract

pub_date

1993-02-01 00:00:00

pages

607-14

issue

4

eissn

0167-4412

issn

1573-5028

journal_volume

21

pub_type

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