Abstract：
:Limonium bicolor, a typical recretohalophyte that lives in saline environments, excretes excessive salt to the environment through epidermal salt glands to avoid salt stress. The aim of this study was to screen for L. bicolor genes involved in salt secretion by high-throughput RNA sequencing. We established the experimental procedure of salt secretion using detached mature leaves, in which the optimal salt concentration was determined as 200 mM NaCl. The detached salt secretion system combined with Illumina deep sequencing were applied. In total, 27,311 genes were annotated using an L. bicolor database, and 2040 of these genes were differentially expressed, of which 744 were up-regulated and 1260 were down-regulated with the NaCl versus the control treatment. A gene ontology enrichment analysis indicated that genes related to ion transport, vesicles, reactive oxygen species scavenging, the abscisic acid-dependent signaling pathway and transcription factors were found to be highly expressed under NaCl treatment. We found that 102 of these genes were likely to be involved in salt secretion, which was confirmed using salt-secretion mutants. The present study identifies the candidate genes in the L. bicolor salt gland that are highly associated with salt secretion. In addition, a salt-transporting pathway is presented to explain how Na(+) is excreted by the salt gland in L. bicolor. These findings will shed light on the molecular mechanism of salt secretion from the salt glands of plants.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Yuan F,Lyu MJ,Leng BY,Zhu XG,Wang BSdoi
10.1007/s11103-016-0460-0subject
Has Abstractpub_date
2016-06-01 00:00:00pages
241-56issue
3eissn
0167-4412issn
1573-5028pii
10.1007/s11103-016-0460-0journal_volume
91pub_type
杂志文章abstract：:We have compared physical and genetic maps of the region around the legJ gene in pea. In this vicinity there are four B-type legumin genes, arranged as two close pairs. The detection of a recombination event within this gene cluster allows the orientation of this group of genes within the surrounding linkage group to ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00038999
更新日期：1993-04-01 00:00:00
abstract：:We have previously shown that mRNA and protein encoded by the Pvlea-18 gene from Phaseolus vulgaris L., a member of a new family of late embryogenesis-abundant (LEA) proteins, accumulate in dark-grown bean seedlings not only in response to water deficit but also during optimal irrigation. In this work, we studied Pvle...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1010607223533
更新日期：2001-03-01 00:00:00
abstract：:Mitochondrial maturation during imbibition of rice embryos follows the transition of unstructured double membrane bound pro-mitochondria to the typical cristae-rich mitochondrial structures observed in mature plant cells. During the first 48 h following imbibition, an ordered increase in the abundance of transcripts e...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-005-3688-7
更新日期：2006-01-01 00:00:00
abstract：:Recent studies have revealed high sequence similarity between homospermidine synthase (HSS), the first pathway-specific enzyme in the biosynthesis of pyrrolizidine alkaloids, a class of sporadically occurring plant defence compounds, and deoxyhypusine synthase (DHS), a ubiquitous enzyme involved in the post-translatio...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1023/a:1026597621646
更新日期：2000-11-01 00:00:00
abstract：:We identified a novel nucleoplasm localized protein in Arabidopsis called AT-hook motif nuclear localized protein 1 (AHL1), which was isolated by visual screening of transformants using random GFP::cDNA fusions. AHL1 contains an AT-hook motif and unknown conserved PPC (plants and prokaryotes conserved) domain that inc...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-004-3249-5
更新日期：2004-09-01 00:00:00
abstract：:A chimeric gene consisting of a bean (Phaseolus vulgaris L.) chalcone synthase (CHS) promoter fused to a bacterial chloramphenicol acetyltransferase (CAT) reporter gene was strongly expressed, and further induced by fungal elicitor, when electroporated into alfalfa (Medicago sativa L.) suspension cell protoplasts. Fun...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00015079
更新日期：1991-05-01 00:00:00
abstract：:Sugarcane (Saccharum spp. hybrids) is a major feedstock for commercial bioethanol production. The recent integration of conversion technologies that utilize lignocellulosic sugarcane residues as well as sucrose from stem internodes has elevated bioethanol yields. RNAi suppression of lignin biosynthetic enzymes is a su...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0527-y
更新日期：2016-11-01 00:00:00
abstract：:The root knot nematode resistance gene Mi in tomato has been mapped in the pericentromeric region of chromosome 6. With the objective of isolating Mi through a map-based cloning approach, we have previously identified and ordered into a high-resolution genetic linkage map a variety of tightly linked molecular markers....
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00021430
更新日期：1993-10-01 00:00:00
abstract：:Telomeres, the complex nucleoprotein structures at the ends of linear eukaryotic chromosomes, along with telomerase, the enzyme that synthesizes telomeric DNA, are required to maintain a stable genome. Together, the enzyme and substrate perform this essential service by protecting chromosomes from exonucleolytic degra...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1023/a:1014091032750
更新日期：2002-03-01 00:00:00
abstract：:We isolated a rice cDNA encoding nucleoside diphosphate kinase (NDK, EC 2.7.4.6). The deduced amino acid sequence of the rice NDK shows highest homology to spinach NDK-I. The rice NDK gene exhibits a strong codon bias (73.8% GC) in the third position of the codon. DNA blot analysis indicated that at least single NDK g...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00021824
更新日期：1993-12-01 00:00:00
abstract：:A cDNA (WPRP1) encoding a wheat proline-rich protein has been isolated and sequenced. The amino acid composition shows 45% proline, with high levels of methionine, lysine and glutamic acid. The derived 378 residue amino acid sequence has a highly repetitive structure which is unlike those of other proline-rich protein...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00023430
更新日期：1991-04-01 00:00:00
abstract：:The embryo essential gene EMB506 plays a crucial role in the transition of the Arabidopsis embryo from radial symmetry to bilateral symmetry just prior to the early heart stage of development. In addition to influencing embryo development EMB506 also affects chloroplast biogenesis. To further investigate the role of E...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-006-6268-6
更新日期：2006-05-01 00:00:00
abstract：:Clones encoding beta-1,3-glucanase have been isolated from a Hevea cDNA library prepared from the latex of Hevea brasiliensis using a probe Nicotiana plumbaginifolia cDNA encoding beta-1,3-glucanase, gnl. Nucleotide sequence analysis showed that a 1.2 kb Hevea cDNA encoding a basic beta-1,3-glucanase showed 68% nucleo...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00043663
更新日期：1995-10-01 00:00:00
abstract：:In bacterial and animal coenzyme A (CoA) biosynthesis, pantothenate kinase (PANK) activity is critical in regulating intracellular CoA levels. Less is known about the role of PANK in plants, although a single plant isozyme from Arabidopsis, AtPANK1, was previously cloned and analyzed in vitro. We report here the chara...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-006-0037-4
更新日期：2006-07-01 00:00:00
abstract：:The gene coding for a new class of proteins rich in glycine and proline (GPRP) was cloned in Arabidopsis thaliana. In the protein sequence, five amino acids - glycine, proline, alanine, tyrosine and histidine - account for 79.4% of the total composition. The protein has two different glycine-rich domains interrupted b...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00049336
更新日期：1996-02-01 00:00:00
abstract：:Salicylic acid (SA) is known to play an important role in the interaction between plant and micro-organisms, both symbiotic and pathogen. In particular, high levels of SA block nodule formation and mycorrhizal colonization in plants. A mutant of Lotus japonicus, named Ljsym4-2, was characterized as unable to establish...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-009-9585-8
更新日期：2010-03-01 00:00:00
abstract：:Golgins are a family of coiled-coil proteins that are associated with the Golgi apparatus. They are necessary for tethering events in membrane fusion and may act as structural support for Golgi cisternae. Here we report on the identification of an Arabidopsis golgin which is a homologue of CASP, a known transmembrane ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-005-4618-4
更新日期：2005-05-01 00:00:00
abstract：:Plant cells respond to cold stress via a regulatory mechanism leading to enhanced cold acclimation accompanied by growth retardation. The C-repeat binding factor (CBF) signaling pathway is essential for cold response of flowering plants. Our previously study documented a novel CBF-like gene from the cold-tolerant Caps...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-014-0181-1
更新日期：2014-06-01 00:00:00
abstract：KEY MESSAGE:An exhaustive analysis of a group of closely related parasitic plants shows a predominantly gradual reduction in plastid genome composition and provides the most reduced plastomes in the genus Cuscuta. Parasitic plants have a diminished to completely absent reliance on photosynthesis, and are characterized ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-019-00884-0
更新日期：2019-08-01 00:00:00
abstract：:Gibberellin (GA) 20-oxidase (GA20ox) is a key enzyme that normally catalyzes the penultimate steps in GA biosynthesis. One of the GA20ox genes in rice (Oryza sativa L.), OsGA20ox2 ( SD1 ), is well known as the "Green Revolution gene", and loss-of function mutation in this locus causes semi-dwarfism. Another GA20ox gen...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-004-1692-y
更新日期：2004-07-01 00:00:00
abstract：:In mammalian cells the product of the human retinoblastoma tumour suppressor gene (pRb) can recruit Rpd3-like histone deacetylases to repress transcription. In this study, we investigated whether this mechanism might also be relevant in plants and found both conserved and distinct features. The expression profiles of ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1022090916446
更新日期：2003-02-01 00:00:00
abstract：:Chloroplast and cytosolic triosephosphate isomerases from spinach were separated and purified to homogeneity. Both enzymes were partially sequenced by Edman degradation. Using degenerate primers designed against the amino acid sequences, a homologous probe for the chloroplast enzyme was amplified and used to isolate s...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019506
更新日期：1994-12-01 00:00:00
abstract：:We have isolated and analysed a 2 kb region of the mitochondrial genome of Arabidopsis thaliana (Columbia) showing a high level of nucleotide identity with the mitochondrial (mt) rps14 small-subunit ribosomal protein gene from Oenothera berteriana and Vicia faba, as well as with an open reading frame (ORF) located ups...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00028903
更新日期：1992-12-01 00:00:00
abstract：:The 22 kDa auxin-binding proteins in higher plants have received considerable attention as candidates for an auxin receptor. A cDNA clone Ca-ERabp1 of hot pepper (Capsicum annum) was isolated using the oligonucleotides as PCR primers. The cDNA codes for a polypeptide related to the major 22 kDa auxin-binding protein f...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020496
更新日期：1996-12-01 00:00:00
abstract：:Genomic variation in S1 and S2 homologous sequences, defined as the S regions, were examined in mitochondrial DNAs of 12 normal cytoplasm maize lines collected in the United States. Three genomic variants were detected among the 12 cytoplasms, eight of which were identical to the Wf9 model structure. Hybridization dat...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF01578377
更新日期：1983-07-01 00:00:00
abstract：:A cDNA subtraction and differential hybridization strategy was used to isolate cDNAs expressed early during male gametophyte development in the important crop species Brassica napus. Three cDNAs, corresponding to genes highly and specifically expressed at the tetrad and microspore stages, are presented here. The analy...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006282507095
更新日期：1999-07-01 00:00:00
abstract：KEY MESSAGE:We reported the functional characterization of cDNAs encoding short-chain isoprenyl diphosphate synthases that control the partitioning of precursors for lavender terpenoids. Lavender essential oil is composed of regular and irregular monoterpenes, which are derived from linear precursors geranyl diphosphat...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-020-00962-8
更新日期：2020-03-01 00:00:00
abstract：:We have cloned and sequenced a full-length cDNA from barley (Hordeum vulgare L.) seeds encoding the bifunctional α-amylase/subtilisin inhibitor (BASI). The nucleotide sequence predicts an open reading frame coding for a protein of 203 amino acids. The first 22 amino acids exhibit the sequence characteristic of a signa...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00044158
更新日期：1989-06-01 00:00:00
abstract：:In planta quantitative studies of cell cycle are necessary for examining the role of cell division in the response of plants to environmental conditions and to analyse the behaviour of transformed plants in this context. We present and discuss non-intrusive kinematic methods which allow estimating the duration of cell...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1023/a:1006438321386
更新日期：2000-08-01 00:00:00
abstract：KEY MESSAGE:A novel open reading frame (ORF) identified and cloned from the A4 cytoplasm of Cajanus cajanifolius induced partial to complete male sterility when introduced into Arabidopsis and tobacco. Pigeonpea (Cajanus cajan L. Millsp.) is the only legume known to have commercial hybrid seed technology based on cytop...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-018-0728-7
更新日期：2018-05-01 00:00:00
