Acidic and basic class III chitinase mRNA accumulation in response to TMV infection of tobacco.

Abstract:

:Complementary DNA clones encoding acidic and basic isoforms of the class III chitinase were isolated from Nicotiana tabacum. The clones share ca. 65% identity, are equally homologous to the class III chitinases from cucumber and Arabidopsis, and are members of small gene families in tobacco. An acidic class III chitinase was purified from the intercellular fluid of tobacco leaves infected with tobacco mosaic virus (TMV). Partial amino acid sequencing of the protein confirmed that it was encoded by one of the cDNA clones. The mRNAs of the class III chitinases are coordinately expressed in response to TMV infection, both in infected and uninfected tissue. The acidic and basic class III chitinases constitute previously undescribed pathogenesis-related proteins in tobacco.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Lawton K,Ward E,Payne G,Moyer M,Ryals J

doi

10.1007/BF00027070

subject

Has Abstract

pub_date

1992-08-01 00:00:00

pages

735-43

issue

5

eissn

0167-4412

issn

1573-5028

journal_volume

19

pub_type

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