Abstract:
:Complementary DNA clones encoding acidic and basic isoforms of the class III chitinase were isolated from Nicotiana tabacum. The clones share ca. 65% identity, are equally homologous to the class III chitinases from cucumber and Arabidopsis, and are members of small gene families in tobacco. An acidic class III chitinase was purified from the intercellular fluid of tobacco leaves infected with tobacco mosaic virus (TMV). Partial amino acid sequencing of the protein confirmed that it was encoded by one of the cDNA clones. The mRNAs of the class III chitinases are coordinately expressed in response to TMV infection, both in infected and uninfected tissue. The acidic and basic class III chitinases constitute previously undescribed pathogenesis-related proteins in tobacco.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Lawton K,Ward E,Payne G,Moyer M,Ryals Jdoi
10.1007/BF00027070subject
Has Abstractpub_date
1992-08-01 00:00:00pages
735-43issue
5eissn
0167-4412issn
1573-5028journal_volume
19pub_type
杂志文章abstract::Cold acclimation in plants is a polygenic phenomenon involving increased expression of several genes. The gene products participate either directly or indirectly towards increasing cold tolerance. Evidence of proteins having a direct effect on cold tolerance is emerging but limited. With isolated protoplasts from warm...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1026599028063
更新日期:2000-11-01 00:00:00
abstract::Rice seedlings accumulate stainable amounts of the 104 and 90 kDa polypeptides in response to high temperature stress. We have purified and raised highly specific polyclonal antisera against both of these polypeptides. In western blotting experiments, we find that these proteins are accumulated to different extents in...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00043653
更新日期:1995-10-01 00:00:00
abstract::We identified an open reading frame (ORF) which is located closely behind the gene encoding granule-bound starch synthase (GBSS) of potato (Solanum tuberosum L.). The ORF ends with a perfect 43 bp direct repeat, which carries the stop triplet precisely at the beginning of the second repeat. The deduced protein shows h...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028815
更新日期:1993-02-01 00:00:00
abstract::In animals and fungi, small cysteine-rich proteins called metallothioneins (MTs) play a role in heavy metal tolerance. MT genes have been isolated in plants, but their function remains to be elucidated. We have isolated two distinct Vicia faba MT genes that belong to the type 1 group of plant MT genes in contrast to a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005790927581
更新日期:1997-03-01 00:00:00
abstract::The auxin-binding protein isolated from maize coleoptiles and characterized in detail describes an auxin recognition protein at the outer surface of the plasmalemma which mediates the auxin effect on cell elongation in maize coleoptiles. Its homologue in tobacco mesophyll protoplasts mediates the auxin effect on secre...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/BF00019401
更新日期:1990-06-01 00:00:00
abstract::Approximately two-thirds of the 55 to 60 plastid ribosomal proteins are encoded in the nucleus. Since the protein products of each of these genes are needed in equal amounts for ribosome assembly, their expression may be coordinately regulated by common mechanisms. To begin to understand how the expression of these ge...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019207
更新日期:1992-03-01 00:00:00
abstract::In a recent publication (Plant Molecular Biology 16: 547-565 (1991)), Showalter et al. described the isolation and initial characterization of fifteen extensin and extensin-like tomato cDNAs. These cDNAs were determined to fall into five distinct classes; class I and II clones encoded extensins, class III and V clones...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027342
更新日期:1992-05-01 00:00:00
abstract::The transcription factor ABA-Insensitive5 (ABI5) is a key regulator of ABA signaling and stress response in Arabidopsis seeds and seedlings. Potential ABI5-interacting proteins were identified by a yeast two-hybrid screen; the most prevalent interactors were a family of four highly conserved plant-specific proteins wi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9344-2
更新日期:2008-08-01 00:00:00
abstract::Screening of a lambda gt11 cDNA expression library of Euglena gracilis with antibodies directed against histones H2 from maize resulted in the isolation of a full-length cDNA for a histone H2A. The open-reading frame of 408 bp corresponded to a protein of 136 amino acid residues (14 kDa). Despite the presence of a pol...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00014447
更新日期:1994-03-01 00:00:00
abstract::The promoter region (-309 to +44) of the Brassica napus storage protein gene napA was studied in transgenic tobacco by successive 5' as well as internal deletions fused to the reporter gene GUS (beta-glucuronidase). The expression in the two main tissues of the seed, the endosperm and the embryo, was shown to be diffe...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00041385
更新日期:1996-12-01 00:00:00
abstract::A near full-length cDNA clone (pZRP2) was isolated from a cDNA library constructed from maize root mRNAs. The predicted polypeptide has a calculated molecular mass of 66,975 Da, is largely hydrophilic, and contains 26 repeats of a motif the consensus sequence of which is RKATTSYG[S][D/E][D/E][D/E][D/E][P]. The functio...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005830313272
更新日期:1997-10-01 00:00:00
abstract::The plant senescence syndrome resembles, in many molecular and phenotypic aspects, plant responses to abiotic stresses. Both processes have an enormous negative global agro-economic impact and endanger food security worldwide. Premature plant senescence is the main cause of losses in grain filling and biomass yield du...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-013-0038-z
更新日期:2013-08-01 00:00:00
abstract::The characterization of a non-photosynthetic isoform of NADP-malic enzyme (NADP-ME) from maize roots, which represents nearly 7% of the total soluble protein of this tissue, was performed. The molecular properties of the purified protein, as well as the kinetic parameters determined, indicate that the NADP-ME isoform ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1010665910095
更新日期:2001-03-01 00:00:00
abstract::14-3-3 proteins form a family of highly conserved proteins with central roles in many eukaryotic signalling networks. In plants, they bind to and activate the plasma membrane H+-ATPase, creating a binding site for the phytotoxin fusicoccin. Barley 14-3-3 transcripts accumulate in the epidermis upon inoculation with th...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1014938417267
更新日期:2002-05-01 00:00:00
abstract::Sugarcane (Saccharum spp. hybrids) is a major feedstock for commercial bioethanol production. The recent integration of conversion technologies that utilize lignocellulosic sugarcane residues as well as sucrose from stem internodes has elevated bioethanol yields. RNAi suppression of lignin biosynthetic enzymes is a su...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0527-y
更新日期:2016-11-01 00:00:00
abstract::A cDNA coding for phytocystatin, a protease inhibitor, was isolated from wheat embryos by differential display RT-PCR and the corresponding full-length cDNA (named WC5 for wheat cystatin gene 5) subsequently obtained by RACE. The deduced primary sequence of the protein suggests the presence of a 28 amino acid N-termin...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1019906031305
更新日期:2002-11-01 00:00:00
abstract::Wheat genes are present in physically small, gene-rich regions, interspersed by gene-poor blocks of retrotransposon-like repetitive sequences. One of the largest gene-rich regions is present around fraction length (FL) 0.8 of the short arm of wheat homoeologous group 1 chromosomes and is called '1S0.8 region'. The obj...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1014876409166
更新日期:2002-03-01 00:00:00
abstract::A cDNA subtraction and differential hybridization strategy was used to isolate cDNAs expressed early during male gametophyte development in the important crop species Brassica napus. Three cDNAs, corresponding to genes highly and specifically expressed at the tetrad and microspore stages, are presented here. The analy...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006282507095
更新日期:1999-07-01 00:00:00
abstract::The nucleotide sequence distal to the rRNA operon from maize chloroplasts has been analyzed. It contains genes coding for tRNA(Arg)(ACG) and tRNA(Asn)(GUU). The tRNA(Arg)(ACG) gene, which is separated from the last rRNA gene of the rRNA operon, the 5S rDNA, by an intergenic region of 252 bp, has the same orientation a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020326
更新日期:1986-11-01 00:00:00
abstract::We have sequenced and characterized the complete psbB gene cluster of Chlamydomonas reinhardtii chloroplast DNA. Although the petB and petD genes are located elsewhere, the sequential order of psbB, ORF31, psbN and psbH is identical to that of the psbB operon in higher plants. Also, intergenic non-coding regions are m...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00047405
更新日期:1993-07-01 00:00:00
abstract::Two cultivars of Perilla frutescens, red and green formas are known to differ in anthocyanin accumulation in leaves and stems. cDNA clones encoding the enzymes involved in anthocyanin biosynthesis, chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR), and UDP glucose: flavonoid 3-O...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005959203396
更新日期:1997-12-01 00:00:00
abstract::During early pea fruit growth, the physiological roles of 4-chloroindole-3-acetic acid (4-Cl-IAA) and IAA, natural pea auxins, in regulating gibberellin (GA) 20-oxidase gene expression (PsGA20ox1) were tested with 4-position, ring-substituted auxins that have a range of biological activities (fruit growth). The effect...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1015522404586
更新日期:2002-07-01 00:00:00
abstract::The dominant tobacco mosaic virus (TMV) resistance gene N induces a hypersensitive response upon TMV infection and protects tobacco against systemic spread of the virus. It has been proposed to change disease resistance specificity by reprogramming the expression of resistance genes or their corresponding avirulence g...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0122-4
更新日期:2014-01-01 00:00:00
abstract:KEY MESSAGE:Related motifs strongly increase gene expression when added to an intron located in coding sequences. Many introns greatly increase gene expression through a mechanism that remains elusive. An obstacle to understanding intron-mediated enhancement (IME) has been the difficulty of locating the specific intron...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0516-1
更新日期:2016-10-01 00:00:00
abstract::Endogenous, 14 kb double-stranded RNAs (dsRNAs) have been found in two ecospecies of cultivated rice (temperate japonica rice and tropical japonica rice, Oryza sativa L.) and in wild rice (O. rufipogon, an ancestor of O. sativa). A comparison of the nucleotide and deduced amino acid sequences of the core regions of th...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006118304093
更新日期:1999-04-01 00:00:00
abstract::The functional homologues of vertebrate natriuretic peptides (NPs), the plant natriuretic peptides (PNPs), are a novel class of peptidic hormones that signal via guanosine 3',5'-cyclic monophosphate (cGMP) and systemically affect plant salt and water balance and responses to biotrophic plant pathogens. Although there ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0465-8
更新日期:2016-06-01 00:00:00
abstract::Transgenic plants carrying chimeric genes composed of segments of the 5'-flanking region of the Arabidopsis 9-tubulin gene (TUB9) fused to the coding region of the beta-glucuronidase (GUS) gene of Escherichia coli were used to investigate the temporal and spatial patterns of TUB9 expression. Chimeric genes that contai...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1011628024798
更新日期:2001-10-01 00:00:00
abstract::We report the isolation and characterisation of a wound-induced cDNA designated AoPR1 from a suspension of mesophyll cells that had been mechanically isolated from cladodes of light-grown Asparagus officinalis seedlings by grinding in a mortar and pestle. The transcript abundance is up-regulated following cell separat...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00026782
更新日期:1992-07-01 00:00:00
abstract::StCDPK1 is a calcium dependent protein kinase expressed in tuberizing potato stolons and in sprouting tubers. StCDPK1 genomic sequence contains eight exons and seven introns, the gene structure is similar to Arabidopsis, rice and wheat CDPKs belonging to subgroup IIa. There is one copy of the gene per genome and it is...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-009-9462-5
更新日期:2009-05-01 00:00:00
abstract::The maize (Zea mays L.) caffeic acid O-methyl-transferase (COMT) is a key enzyme in the biosynthesis of lignin. In this work we have characterized the involvement of COMT in the lignification process through the study of the molecular mechanisms involved in its regulation. The examination of the maize COMT gene promot...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-9058-2
更新日期:2006-12-01 00:00:00