Characterization of the expression of a wheat cystatin gene during caryopsis development.

Abstract:

:A cDNA coding for phytocystatin, a protease inhibitor, was isolated from wheat embryos by differential display RT-PCR and the corresponding full-length cDNA (named WC5 for wheat cystatin gene 5) subsequently obtained by RACE. The deduced primary sequence of the protein suggests the presence of a 28 amino acid N-terminal signal sequence and a 100 amino acid mature protein containing the three consensus motifs known to interact with the active site of cysteine peptidases. Northern and western analysis revealed a spatio-temporal pattern of the cystatin gene expression during caryopse development. In the embryo, WC5 was only expressed during early embryogenesis whereas, in seed covering layers, WC5 expression was restricted to the maturation stage of grain development. In addition, immunolocalization experiments showed that cystatin accumulated in the aleurone layer of the maturating seed and in the parenchymal tissues of the embryo scutellum. A recombinant form of the wheat cystatin was shown to be able to inhibit peptidase activities present in whole seed protein extracts. In addition, immunological techniques allowed us to identify two putative target peptidases. The possible roles of the cystatin protein are discussed in relation with tissular localization and putative peptidase targets during seed maturation.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Corr-Menguy F,Cejudo FJ,Mazubert C,Vidal J,Lelandais-Brière C,Torres G,Rode A,Hartmann C

doi

10.1023/a:1019906031305

subject

Has Abstract

pub_date

2002-11-01 00:00:00

pages

687-98

issue

4-5

eissn

0167-4412

issn

1573-5028

journal_volume

50

pub_type

杂志文章
  • Post-transcriptional gene silencing mutants.

    abstract::It has been known for more than a decade that increasing the gene copy number does not necessarily lead to increased gene activity. Plants have developed efficient mechanisms such as post-transcriptional gene silencing (PTGS) to regulate abnormal gene expression in a sequence-specific fashion. PTGS of (trans)genes can...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审

    doi:10.1023/a:1006499832424

    authors: Morel JB,Vaucheret H

    更新日期:2000-06-01 00:00:00

  • A census of carbohydrate-active enzymes in the genome of Arabidopsis thaliana.

    abstract::The synthesis, modification, and breakdown of carbohydrates is one of the most fundamentally important reactions in nature. The structural and functional diversity of glycosides is mirrored by a vast array of enzymes involved in their synthesis (glycosyltransferases), modification (carbohydrate esterases) and breakdow...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:

    authors: Henrissat B,Coutinho PM,Davies GJ

    更新日期:2001-09-01 00:00:00

  • Molecular characterization of rice hsp101: complementation of yeast hsp104 mutation by disaggregation of protein granules and differential expression in indica and japonica rice types.

    abstract::HSP100 protein is an important component of the heat-shock response in diverse organisms. Using specific primers based on cDNA sequence, rice hsp101 gene was PCR-amplified and sequenced. Southern analysis revealed that there appears to be a single gene per haploid genome coding for HSP101 protein in rice. Northern ana...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1022324920316

    authors: Agarwal M,Sahi C,Katiyar-Agarwal S,Agarwal S,Young T,Gallie DR,Sharma VM,Ganesan K,Grover A

    更新日期:2003-03-01 00:00:00

  • Differential display-mediated rapid identification of different members of a multigene family, HSP 16.9 in wheat.

    abstract::Isolation of cDNAs encoding individual members of a gene family is essential for assessing their role in a biological phenomenon. However, this process is often laborious and slow due to highly conserved protein-coding region that interferes with the isolation of the individual members. Identification of gene-specific...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00042230

    authors: Joshi CP,Nguyen HT

    更新日期:1996-06-01 00:00:00

  • Unusual sequence of an abscisic acid-inducible mRNA which accumulates late in Brassica napus seed development.

    abstract::We have analyzed the nucleotide sequence and accumulation of an mRNA which is prevalent in seeds of Brassica napus L. During normal development, the mRNA begins to accumulate during late embryogeny, is stored in dry seeds, and becomes undetectable in seedlings within 24 hours after imbibition. Moreover, abscisic acid ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00017579

    authors: Harada JJ,Delisle AJ,Baden CS,Crouch ML

    更新日期:1989-04-01 00:00:00

  • Functional analysis of a complex oncogene arrangement in biotype III Agrobacterium tumefaciens strains.

    abstract::The ubiquitous grapevine-associated octopine/cucumopine Ti plasmids of biotype III Agrobacterium tumefaciens strains carry two T regions, TA and TB, with a complex oncogene arrangement. Within the octopine/cucumopine group, two main strain types were identified: 'large TA' strains with a TA region resembling the TL re...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00018558

    authors: Huss B,Tinland B,Paulus F,Walter B,Otten L

    更新日期:1990-02-01 00:00:00

  • Direct interaction between the tobacco mosaic virus helicase domain and the ATP-bound resistance protein, N factor during the hypersensitive response in tobacco plants.

    abstract::Plants cope with pathogens with distinct mechanisms. One example is a gene-for-gene system, in which plants recognize the pathogen molecule by specified protein(s), this being called the R factor. However, mechanisms of interaction between proteins from the host and the pathogen are not completely understood. Here, we...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-005-5817-8

    authors: Ueda H,Yamaguchi Y,Sano H

    更新日期:2006-05-01 00:00:00

  • Organization and expression of a phycobiliprotein gene cluster from the unicellular red alga Cyanidium caldarium.

    abstract::We have sequenced a plastid gene cluster from the unicellular red alga Cyanidium caldarium which is located downstream from the psbA gene and contains, in the following order, genes for a beta-allophycocyanin-like protein (apcB'), a putative 9.5 kDa allophycocyanin linker protein (apcL9.5) and a putative 29 kDa phycoc...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00014494

    authors: Valentin K,Maid U,Emich A,Zetsche K

    更新日期:1992-10-01 00:00:00

  • Plant viral leaders influence expression of a reporter gene in tobacco.

    abstract::In order to optimise expression of a foreign protein in transgenic plants we investigated the potential benefits of including a viral untranslated leader sequence within a plant transformation vector. A variety of 5 leaders, including the tobacco mosaic virus (TMV) leader sequence and 31 nucleotides of the cauliflower...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00021423

    authors: Dowson Day MJ,Ashurst JL,Mathias SF,Watts JW,Wilson TM,Dixon RA

    更新日期:1993-10-01 00:00:00

  • Phylogenetic origin of a secondary pathway: the case of pyrrolizidine alkaloids.

    abstract::Recent studies have revealed high sequence similarity between homospermidine synthase (HSS), the first pathway-specific enzyme in the biosynthesis of pyrrolizidine alkaloids, a class of sporadically occurring plant defence compounds, and deoxyhypusine synthase (DHS), a ubiquitous enzyme involved in the post-translatio...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审

    doi:10.1023/a:1026597621646

    authors: Ober D,Hartmann T

    更新日期:2000-11-01 00:00:00

  • Over-expression of a C-terminal region of phytochrome B.

    abstract::In an attempt to identify domains directly involved in the signal transduction of phytochrome B (phyB), we over-expressed the achromophoric C-terminal half of phyB under control of the CaMV-35S promoter in transgenic Arabidopsis. In three independent transgenic lines, we detected accumulation of the introduced protein...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00040726

    authors: Sakamoto K,Nagatani A

    更新日期:1996-08-01 00:00:00

  • Characterisation of transcript initiation sites in ribosome-deficient barley plastids.

    abstract::Transcription of plastid genes in higher plants is driven by two RNA polymerases. One is encoded in the chloroplast, the other is encoded in the nucleus. RNA synthesis in ribosome-deficient plastids is performed exclusively by the nuclear-encoded enzyme. In vitro capping was used to identify the transcriptional start ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1005924502336

    authors: Hübschmann T,Börner T

    更新日期:1998-02-01 00:00:00

  • Phytohormone-regulated beta-amylase gene expression in rice.

    abstract::The expression of beta-amylase genes in rice (Oryza sativa) and its regulation by phytohormones gibberellic acid (GA) and abscisic acid (ABA) were examined. Upon germination beta-amylase is synthesized de novo in aleurone cells and (GA) is not required. Exogenous addition of GA does not enhance the beta-amylase activi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00040716

    authors: Wang SM,Lue WL,Eimert K,Chen J

    更新日期:1996-08-01 00:00:00

  • Producing low-caffeine tea through post-transcriptional silencing of caffeine synthase mRNA.

    abstract::In this study, attempt has been made to produce a selected cultivar of tea with low-caffeine content using RNAi technology. The caffeine biosynthetic pathway in tea has been proposed to involve three N-methyltransferases such as xanthosine methyltransferase, 7-N-methylxanthine methyltransferase and 3, 7-dimethylxanthi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-011-9785-x

    authors: Mohanpuria P,Kumar V,Ahuja PS,Yadav SK

    更新日期:2011-08-01 00:00:00

  • Molecular characterization of His-Asp phosphorelay signaling factors in maize leaves: implications of the signal divergence by cytokinin-inducible response regulators in the cytosol and the nuclei.

    abstract::Genes for histidyl-aspartyl (His-Asp) phosphorelay components (His-containing phosphotransfer proteins, HP, and response regulators, RR) were isolated from Zea mays L. to characterize their function in cytokinin signaling. Six type-A RRs (ZmRR1, ZmRR2, ZmRR4-ZmRR7), 3 type-B RRs (ZmRR8-ZmRR10), and 3 HPs (ZmHP1-ZmHP3)...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1023971315108

    authors: Asakura Y,Hagino T,Ohta Y,Aoki K,Yonekura-Sakakibara K,Deji A,Yamaya T,Sugiyama T,Sakakibara H

    更新日期:2003-05-01 00:00:00

  • Characteristics of RNA silencing in plants: similarities and differences across kingdoms.

    abstract::RNA silencing is a collective term that encompasses the sequence of events that leads to the targeted degradation of cellular mRNA and thus to the silencing of corresponding gene expression. RNA silencing is initiated after introduction into the host genome of a gene that is homologous to an endogenous gene. Transcrip...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审

    doi:10.1023/B:PLAN.0000028797.63168.a7

    authors: Susi P,Hohkuri M,Wahlroos T,Kilby NJ

    更新日期:2004-01-01 00:00:00

  • Characterization of pectinases and pectin methylesterase cDNAs in pods of green beans (Phaseolus vulgaris L.).

    abstract::Tomato fruit maturation is accompanied by a depolymerization of cell wall pectins which is due to the action of endopolygalacturonase (endoPG) preceded by pectin methylesterase (PE) activity. To investigate the role of endoPG and PE in determining the structure of green bean (Phaseolus vulgaris L.) pectins, these pect...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00040831

    authors: Ebbelaar ME,Tucker GA,Laats MM,van Dijk C,Stolle-Smits T,Recourt K

    更新日期:1996-09-01 00:00:00

  • Characterization of a family of genes encoding a fruit-specific wound-stimulated protein of bell pepper (Capsicum annuum): identification of a new family of transposable elements.

    abstract::Using a fruit-specific cDNA as a probe we isolated and sequenced the two corresponding homologous genes (Sn-1 and Sn-2) of the bell pepper (Capsicum annuum) genome. Both genes have a single intron and numerous unusual long inverted repeat sequences. The introns share 87% homology and Sn-2 contains one 450 bp additiona...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00032663

    authors: Pozueta-Romero J,Klein M,Houlné G,Schantz ML,Meyer B,Schantz R

    更新日期:1995-09-01 00:00:00

  • Expression characteristics of OS-ACS1 and OS-ACS2, two members of the 1-aminocyclopropane-1-carboxylate synthase gene family in rice (Oryza sativa L. cv. Habiganj Aman II) during partial submergence.

    abstract::Deepwater rice can grow in the regions of Southeast Asia that are flooded during the monsoon season because it has several adaptations allowing it to survive under flooded conditions. One such adaptation is the ability for rapid internode elongation upon partial submergence to maintain its foliage above the rising flo...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/b:plan.0000009693.26740.c3

    authors: Zarembinski TI,Theologis A

    更新日期:1997-01-01 00:00:00

  • Tobacco plants transformed with cdc25, a mitotic inducer gene from fission yeast.

    abstract::We investigated the effects of expressing a cDNA of cdc25, a mitotic inducer gene of Schizosaccharomyces pombe, on the development of transgenic tobacco plants (Nicotiana tabacum cv. Samsun). Nine independent primary transformants were regenerated containing the cdc25 sequence under the control of a cauliflower mosaic...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00019293

    authors: Bell MH,Halford NG,Ormrod JC,Francis D

    更新日期:1993-11-01 00:00:00

  • An upstream region of the Arabidopsis thaliana CDKA;1 (CDC2aAt) gene directs transcription during trichome development.

    abstract::The cell cycle of eukaryotes is tightly regulated through the activity of cyclin-dependent kinases. The Arabidopsis thaliana CDKA;1 (CDC2aAt) gene is thought to encode such a protein kinase, since it is actively transcribed in proliferating tissues and can complement defects in the Schizosaccharomyces pombe cdc2 gene....

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1010665831955

    authors: Imajuku Y,Ohashi Y,Aoyama T,Goto K,Oka A

    更新日期:2001-05-01 00:00:00

  • Acidic and basic class III chitinase mRNA accumulation in response to TMV infection of tobacco.

    abstract::Complementary DNA clones encoding acidic and basic isoforms of the class III chitinase were isolated from Nicotiana tabacum. The clones share ca. 65% identity, are equally homologous to the class III chitinases from cucumber and Arabidopsis, and are members of small gene families in tobacco. An acidic class III chitin...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00027070

    authors: Lawton K,Ward E,Payne G,Moyer M,Ryals J

    更新日期:1992-08-01 00:00:00

  • Genetic integration of DORNRÖSCHEN and DORNRÖSCHEN-LIKE reveals hierarchical interactions in auxin signalling and patterning of the Arabidopsis apical embryo.

    abstract::DORNRÖSCHEN (DRN) and DORNRÖSCHEN-LIKE (DRNL) encode AP2-domain transcription factors, which act redundantly in cotyledon organogenesis. A more detailed genetic study now integrates DRN and DRNL into the CUP-SHAPED COTYLEDON (CUC) regulatory network and places DRN and DRNL differentially within the auxin signalling ne...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-010-9721-5

    authors: Chandler JW,Cole M,Jacobs B,Comelli P,Werr W

    更新日期:2011-02-01 00:00:00

  • Eucalypt alpha-tubulin: cDNA cloning and increased level of transcripts in ectomycorrhizal root system.

    abstract::Because symbionts are experiencing major morphological changes during ectomycorrhiza development, the expression of genes encoding cytoskeletal proteins is likely altered. To test this contention, we have cloned and characterized in a alpha-tubulin cDNA (EgTubA1) from Eucalyptus globulus. A poorly-aggressive isolate (...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00019477

    authors: Diaz EC,Martin F,Tagu D

    更新日期:1996-07-01 00:00:00

  • Characterization and comparison of intron structure and alternative splicing between Medicago truncatula, Populus trichocarpa, Arabidopsis and rice.

    abstract::Alignment of transcripts and genome sequences yielded a set of alternatively spliced transcripts in four angiosperm genomes: three dicotyledon species Medicago truncatula (Medicago), Populus trichocarpa (poplar) and Arabidopsis thaliana (Arabidopsis), and the monocotyledon Oryzae sativa (rice). Intron retention was th...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-008-9334-4

    authors: Baek JM,Han P,Iandolino A,Cook DR

    更新日期:2008-07-01 00:00:00

  • Differential expression of oxygen-evolving polypeptide genes in maize leaf cell types.

    abstract::Three polypeptides of 33 kD, 23 kD and 16 kD are released from maize photosystem II particles by alkaline Tris solution treatment and shown to cross-react with antisera to purified spinach oxygen-evolving (OE) polypeptides of 34 kD, 23 kD and 17 kD, respectively. They are not located exclusively in mesophyll cells but...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00166458

    authors: Sheen JY,Sayre RT,Bogorad L

    更新日期:1987-05-01 00:00:00

  • The 22 bp W1 element in the pea lectin promoter is necessary and, as a multimer, sufficient for high gene expression in tobacco seeds.

    abstract::The pea lectin (Psl) gene encodes an abundant seed protein. Its seed-specific expression pattern is conserved in transgenic tobacco plants. Progressive 5' promoter deletions resulted in a gradual decrease of transcriptional activity in tobacco seed. A fragment of 115 bp still conferred seed-specific expression albeit ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00019103

    authors: de Pater S,Pham K,Klitsie I,Kijne J

    更新日期:1996-11-01 00:00:00

  • Suppression of phenylalanine ammonia lyase expression in sugar beet by the fungal pathogen Cercospora beticola is mediated at the core promoter of the gene.

    abstract::The suppression of plant defence reactions plays a crucial role in causing plant diseases. In this report, we show that inducible plant defences are repressed during the development of Cercospora leaf spot disease. In the early phase of infection of sugar beet (Beta vulgaris L.) leaves with the phytopathogenic fungus ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-004-2141-7

    authors: Schmidt K,Heberle B,Kurrasch J,Nehls R,Stahl DJ

    更新日期:2004-08-01 00:00:00

  • Serial analysis of gene expression (SAGE) of host-plant resistance to the cassava mosaic disease (CMD).

    abstract::Cassava mosaic disease (CMD) is a viral disease of the important tropical staple crop cassava (Manihot esculenta) and preferred management involves use of host-plant resistance. The best available resistance is controlled by a single dominant gene. Serial analysis of gene expression (SAGE) was used to analyze the gene...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-004-3477-8

    authors: Fregene M,Matsumura H,Akano A,Dixon A,Terauchi R

    更新日期:2004-11-01 00:00:00

  • Bacteroid-encoded proteins are secreted into the peribacteroid space by Rhizobium leguminosarum.

    abstract::Bacteroids of Rhizobium leguminosarum in root nodules of Pisum sativum are enclosed by a plant-derived peribacteriod membrane (PBM). The contents of the interstitial peribacteroid space (PBS) between bacteroid membrane and PBM were isolated by a controlled osmotic shock of PBM-enclosed bacteroids and analysed by two-d...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00015670

    authors: Katinakis P,Lankhorst RM,Louwerse J,van Kammen A,van den Bos RC

    更新日期:1988-03-01 00:00:00