Abstract：
:We investigated the effects of expressing a cDNA of cdc25, a mitotic inducer gene of Schizosaccharomyces pombe, on the development of transgenic tobacco plants (Nicotiana tabacum cv. Samsun). Nine independent primary transformants were regenerated containing the cdc25 sequence under the control of a cauliflower mosaic virus 35S gene promoter. Eight of the nine plants showed altered leaf morphology, the lamina being lengthened and twisted and the interveinal regions being pocketed. One of these was sacrificed for analysis of the root meristem, where the cells were found to be significantly smaller than in the wild type. The other seven were grown on and showed precocious flowering, flowers being produced earlier and in significantly greater numbers than in the wild type. They also developed abnormal flowers on short stalks developing in a position normally occupied by the most proximal axillary bud of otherwise normal flower pedicels. The presence or absence of these phenotypes in the primary transformants and in the T2 generation was associated with the presence or absence of detectable levels of cdc25 transcripts.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Bell MH,Halford NG,Ormrod JC,Francis Ddoi
10.1007/BF00019293subject
Has Abstractpub_date
1993-11-01 00:00:00pages
445-51issue
3eissn
0167-4412issn
1573-5028journal_volume
23pub_type
杂志文章abstract：:Several signal transduction pathways play important roles in the sexual life cycle of Chlamydomonas. Nitrogen deprivation, perhaps sensed as a drop in intracellular [NH4+], triggers a signal transduction pathway that results in altered gene expression and the induction of the gametogenic pathway. Blue light triggers a...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1007/BF00016474
更新日期：1994-12-01 00:00:00
abstract：:Infection of tobacco by tobacco mosaic virus (TMV) induces coordinate expression of genes encoding acidic and basic beta-1,3-glucanase isoforms. These genes are differentially expressed in response to other treatments. Salicylate treatment induces acidic glucanase mRNA to a higher level than basic glucanase mRNA. Ethy...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00028803
更新日期：1993-02-01 00:00:00
abstract：:We have cloned and sequenced a full-length cDNA from barley (Hordeum vulgare L.) seeds encoding the bifunctional α-amylase/subtilisin inhibitor (BASI). The nucleotide sequence predicts an open reading frame coding for a protein of 203 amino acids. The first 22 amino acids exhibit the sequence characteristic of a signa...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00044158
更新日期：1989-06-01 00:00:00
abstract：:The pea lectin (Psl) gene encodes an abundant seed protein. Its seed-specific expression pattern is conserved in transgenic tobacco plants. Progressive 5' promoter deletions resulted in a gradual decrease of transcriptional activity in tobacco seed. A fragment of 115 bp still conferred seed-specific expression albeit ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019103
更新日期：1996-11-01 00:00:00
abstract：:DNA microsatellites are ubiquitously present in eukaryotic genomes and represent a vast source of highly informative markers. We describe in this article a (GGC)n microsatellite which is widely distributed in eukaryotic genomes. Using polymerase chain reaction (PCR) techniques and DNA sequencing, we demonstrated for t...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00014544
更新日期：1993-02-01 00:00:00
abstract：:The characterization of the operon encoding the alpha and beta subunits of rhodophytan (R)-phycoerythrin (PE) from the macrophytic red alga Polysiphonia boldii is reported. This plastid-encoded operon was cloned, its nucleotide sequence determined, and its expression characterized by northern and primer extension anal...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00039617
更新日期：1993-01-01 00:00:00
abstract：:cDNA clones encoding the precursor of the Rieske FeS protein of the chloroplast thylakoid membrane have been isolated from a pea leaf cDNA library in lambda gt 11, following screening with antibodies to purified pea chloroplast Rieske FeS protein. The longest cDNA insert of 880 bp encodes a polypeptide of 230 amino ac...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040617
更新日期：1992-11-01 00:00:00
abstract：KEY MESSAGE:Salt stress triggers a simultaneous transcriptional repression and aquaporin internalization to modify root cell water conductivity. Plasma membrane intrinsic proteins (PIPs) are involved in the adjustment of plant water balance in response to changing environmental conditions. In this study, Arabidopsis wi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0542-z
更新日期：2016-12-01 00:00:00
abstract：:Rice has developed several morphological and physiological strategies to adapt to phosphate starvation in the soil. In order to elucidate the molecular basis of response to phosphate starvation, we performed mRNA sequencing of 4 rice cultivars with variation in growth response to Pi starvation as indicated by the shoo...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-013-0106-4
更新日期：2013-12-01 00:00:00
abstract：:We have cloned and sequenced a gene, pds, from the cyanobacterium Synechococcus PCC7942 that is responsible for resistance to the bleaching herbicide norflurazon. A point mutation in that gene, leading to an amino acid substitution from valine to glycine in its polypeptide product, was found to confer this resistance....
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00016069
更新日期：1991-06-01 00:00:00
abstract：:Soybean is an important crop not only for human consumption but also for its addition of nitrogen to the soil during crop rotation. China has the largest collection of cultivated soybeans (Glycine max) and wild soybeans (Glycine soja) all over the world. The platform of soybean core, mini core and integrated applied c...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1007/s11103-013-0076-6
更新日期：2013-09-01 00:00:00
abstract：:Two types of structurally distinct O-methyltransferases mediate the methylation of hydroxylated monomeric lignin precursors in angiosperms. Caffeate 3-O-methyltransferase (COMT; EC 2.1.1.68) methylates the free acids and caffeoyl CoA 3-O-methyltransferase (CCoAOMT; EC 2.1.1.104) methylates coenzyme A esters. Recently,...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006244325250
更新日期：1999-07-01 00:00:00
abstract：:An important group of splicing factors involved in constitutive and alternative splicing contain an arginine/serine (RS)-rich domain. We have previously demonstrated the existence of such factors in plants and report now on a new family of splicing factors (termed the RSZ family) from Arabidopsis thaliana which additi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006129615846
更新日期：1999-03-01 00:00:00
abstract：:APETALA2 (AP2) transcription factors (TFs) play very important roles in plant growth and development and in defense response. Here, we report functional characterization of four AP2 TF family genes [(RAP2.6 (At1g43160), RAP2.6L (At5g13330), DREB 26 (At1g21910) and DREB19 (At2g38340)] that were identified among NaCl in...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-010-9711-7
更新日期：2011-01-01 00:00:00
abstract：:The actin gene superfamily of Petunia hybrida cv. Mitchell contains greater than 100 gene members which have been divided into several highly divergent subfamilies [1]. Five subfamily-specific probes have been used to compare the actin genes among the Mitchell, Violet 23 (V23) and Red 51 (R51) cultivars of P. hybrida....
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00017466
更新日期：1988-09-01 00:00:00
abstract：:Differentiation of the Arabidopsis thaliana (Arabidopsis) seed coat epidermal cells involves pronounced changes highlighted by the synthesis and secretion of copious amounts of dispensable, pectinaceous mucilage followed by a thick cellulosic secondary cell wall. This cell type, therefore, represents an excellent mole...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-012-9984-0
更新日期：2013-01-01 00:00:00
abstract：:ATP-binding cassette transporters are involved in the active transport of a wide variety of metabolites in prokaryotes and eukaryotes. One subfamily, the Pleiotropic Drug Resistance (PDR) transporters, or full-size ABCG transporters, are found only in fungi and plants. NtPDR1 was originally identified in Nicotiana tab...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-013-0053-0
更新日期：2013-05-01 00:00:00
abstract：:The soybean leghemoglobin lba gene promoter sequence was determined and aligned with the promoter sequence of the soybean lbc3 gene from the same gene family. Five highly conserved regions were found. There are two large conserved regions, one of which overlaps the basic promoter while the other defines a minimal enha...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00027380
更新日期：1993-08-01 00:00:00
abstract：:Live imaging during floral development revealed that expression of the DORNRÖSCHEN-LIKE (DRNL) gene encoding an AP2-like transcription factor, marks all organ founder cells. Transcription precedes the perception of auxin response maxima as measured by the DR5 reporter and is unaffected in early organogenesis, by mutat...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-011-9779-8
更新日期：2011-05-01 00:00:00
abstract：:The mitochondrial DNA molecules of two interfertile algal species, Chlamydomonas smithii and C. reinhardtii, are co-linear except for a 1075 bp intron (the alpha-insert) that is present in the cob gene of C. smithii. The alpha-insert, a group I intron (Cs cob.1) containing an open reading frame (ORF) which encodes a b...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019218
更新日期：1992-03-01 00:00:00
abstract：:Two different calmodulin (CaM) cDNAs (MBCaM-1 and MBCaM-2) were isolated from a vigna radiata lambda gt11 library by screening with a heterologous Arabidopsis cDNA probe (TCH-1). Both cDNAs are 85% homologous inside the coding region but are highly divergent outside this region. The polypeptides encoded by MBCaM-1 and...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00029857
更新日期：1994-03-01 00:00:00
abstract：:Acyl-CoA binding proteins (ACBPs) are small (ca. 10 kDa) highly-conserved cytosolic proteins that bind long-chain acyl-CoAs. A novel cDNA encoding ACBP1, a predicted membrane protein of 24.1 kDa with an acyl-CoA binding protein domain at its carboxy terminus, was cloned from Arabidopsis thaliana. At this domain, ACBP1...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006052108468
更新日期：1998-11-01 00:00:00
abstract：:We investigated the spatial and temporal expression patterns of two rice calcium-dependent protein kinases (CDPKs), OsCDPK2 and OSCDPK11, using isoform-specific antisera. Bands of the expected molecular sizes for OsCDPK2 (59 kDa) and OsCDPK11 (61 kDa) were detected on western blots. OsCDPK2 and OsCDPK11 mRNA and prote...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006316422400
更新日期：1999-12-01 00:00:00
abstract：:Members of the plant-specific GRAS family of putative transcription factors are involved in various aspects of plant development. SCARECROW (SCR) is a member of this protein family and plays a significant role in the radial patterning of both roots and shoots. However, little is known about the regulation of SCR expre...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-004-0892-9
更新日期：2004-05-01 00:00:00
abstract：:Transcription of plastid genes in higher plants is driven by two RNA polymerases. One is encoded in the chloroplast, the other is encoded in the nucleus. RNA synthesis in ribosome-deficient plastids is performed exclusively by the nuclear-encoded enzyme. In vitro capping was used to identify the transcriptional start ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005924502336
更新日期：1998-02-01 00:00:00
abstract：:Transformed root cultures of Nicotiana rustica have been generated in which the gene from the yeast Saccharomyces cerevisiae coding for ornithine decarboxylase has been integrated. The gene, driven by the powerful CaMV35S promoter with an upstream duplicated enhancer sequence, shows constitutive expression throughout ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00017721
更新日期：1990-07-01 00:00:00
abstract：:Complex signal transduction pathways underlie the myriad plant responses to attack by pathogens. Ca(2+) is a universal second messenger in eukaryotes that modulates various signal transduction pathways through stimulus-specific changes in its intracellular concentration. Ca(2+)-binding proteins such as calmodulin (CaM...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-005-8395-x
更新日期：2005-08-01 00:00:00
abstract：:Micropropagated shoots of three forest tree species, poplar (Populus tremula x P. alba), wild cherry (Prunus avium L.) and walnut (Juglans nigra x J. regia), were inoculated each with six different wild-type Agrobacterium strains. Poplar and wild cherry developed tumors that grew hormone-independently, whereas on waln...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040638
更新日期：1991-09-01 00:00:00
abstract：:Dobzhansky stated that nothing in biology makes sense except in the light of evolution. A close corollary, and the central theme of this paper, is that everything makes a lot more sense in the light of phylogeny. Systematics is in the midst of a renaissance, heralded by the widespread application of new analytical app...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：
更新日期：2000-01-01 00:00:00
abstract：:As part of a strategy to determine the precise role of pea (Pisum sativum) lectin, Psl, in nodulation of pea by Rhizobium leguminosarum, mutations were introduced into the genetic determinant for pea lectin by site-directed mutagenesis using PCR. Introduction of a specific mutation, N125D, into a central area of the s...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00028892
更新日期：1992-12-01 00:00:00
