Abstract：
:We investigated the spatial and temporal expression patterns of two rice calcium-dependent protein kinases (CDPKs), OsCDPK2 and OSCDPK11, using isoform-specific antisera. Bands of the expected molecular sizes for OsCDPK2 (59 kDa) and OsCDPK11 (61 kDa) were detected on western blots. OsCDPK2 and OsCDPK11 mRNA and protein levels increased in unison during flower development. However, at the onset of seed development, the protein expression profiles diverged significantly. OsCDPK2 protein was expressed at low levels during early seed development, but increased to high levels that were maintained in later stages (20 days after fertilisation, DAF). Conversely, OsCDPK11 protein levels were high at the beginning of seed development, but fell rapidly from 10 DAF onwards. This decrease in the level of OsCDPK11 protein was associated with the abundant synthesis of a truncated mRNA species. OsCDPK2 expression was also closely associated with light perception. OsCDPK2 protein was barely detectable in green leaves exposed to light, but levels increased sharply when plants were shifted to darkness. Initially, this increase reflected a rapid elevation in the levels of OsCDPK2 mRNA, which was normally located in the mesophyll. Conversely, OsCDPK11 mRNA and protein levels were unaffected by light. These data strongly indicate that two rice CDPK isoforms have different functions in seed development and in response to light in leaves.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Frattini M,Morello L,Breviario Ddoi
10.1023/a:1006316422400subject
Has Abstractpub_date
1999-12-01 00:00:00pages
753-64issue
6eissn
0167-4412issn
1573-5028journal_volume
41pub_type
杂志文章abstract：:A new cytoplasmic male sterile sunflower, CMS3 [44], was characterised in relation to the Petiolaris (PET1) cytoplasmic male-sterile sunflower, CMS89 [25]. Southern blot analysis showed that the mitochondrial genome of CMS3 contains unique rearrangements in at least five loci (atp6, atp9, atpA, nad1 + 5 and coxIII) co...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019495
更新日期：1994-12-01 00:00:00
abstract：:The nucleotide sequence of a cDNA prepared from poly(A)+ RNA from Lycopersicon esculentum fruit codes for a protein, M(r) 20,812, with features representative of the protein core of arabinogalactan proteins. The deduced amino acid sequence resembles that of peptides of arabinogalactan proteins isolated from carrot and...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020254
更新日期：1995-05-01 00:00:00
abstract：:Expression of the light-harvesting complex protein genes (Lhc) is under the control of a circadian clock. To dissect the molecular regulatory components of the circadian clock a promoter deletion analysis of four tomato Lhc genes was performed in transgenic tobacco plants. The important 5'-upstream promoter regions ar...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006094015513
更新日期：1998-11-01 00:00:00
abstract：:Endogenous, 14 kb double-stranded RNAs (dsRNAs) have been found in two ecospecies of cultivated rice (temperate japonica rice and tropical japonica rice, Oryza sativa L.) and in wild rice (O. rufipogon, an ancestor of O. sativa). A comparison of the nucleotide and deduced amino acid sequences of the core regions of th...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006118304093
更新日期：1999-04-01 00:00:00
abstract：:Dehiscence of oilseed rape pods, commonly known as pod shatter, is a process of agronomic importance that results in seed loss causing yield reductions and carry-over of the crop into the following growing season. In an effort to understand the mechanisms underlying this developmental event, the changes in gene expres...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040589
更新日期：1994-01-01 00:00:00
abstract：:Exocarp color of sand pear is an important trait for the fruit production and has caused our concern for a long time. Our previous study explored the different expression genes between the two genotypes contrasting for exocarp color, which indicated the different suberin, cutin, wax and lignin biosynthesis between the...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-014-0173-1
更新日期：2014-05-01 00:00:00
abstract：:Higher plant microspores, when subjected to various stress treatments in vitro, are able to reprogram their regular gametophytic development towards the sporophytic pathway to form haploid embryos and plants. Suppression subtractive hybridization (SSH) and metabolic profiling were used to characterize this development...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-006-9078-y
更新日期：2007-01-01 00:00:00
abstract：:Despite recent progress auxin signal transduction remains largely scetchy and enigmatic. A good body of evidence supports the notion that the ABP1 could be a functional receptor or part of a receptor, respectively, but this is not generally accepted. Evidence for other functional receptors is lacking, as is any clearc...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：
更新日期：2002-06-01 00:00:00
abstract：:Agrobacterium-mediated gene transfer is the method of choice for many plant biotechnology laboratories; however, large-scale use of this organism in conifer transformation has been limited by difficult propagation of explant material, selection efficiencies and low transformation frequency. We have analyzed co-cultiva...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006126609534
更新日期：1999-02-01 00:00:00
abstract：:A full-length cDNA encoding glutamine synthetase was isolated from a lambda gt11 library constructed from the poly(A)+ RNA isolated from lettuce seeds incubated under red light. The nucleotide sequence of the cDNA and the deduced sequence of amino acids showed a high degree of homology to those of the cytosol-type glu...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00036917
更新日期：1990-08-01 00:00:00
abstract：KEY MESSAGE:The simultaneous expression of AmRosea1 and AmDelila transcription factors from snapdragon can activate the anthocyanin pathway in orange carrots, leading to the synthesis and accumulation of anthocyanins in the taproots. Anthocyanins are phenolic compounds produced in various parts of plants. They are used...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-020-01002-1
更新日期：2020-07-01 00:00:00
abstract：KEY MESSAGE:This review contains functional roles of MYB transcription factors in the transcriptional regulation of anthocyanin biosynthesis in horticultural plants. This review describes potential uses of MYB TFs as tools for metabolic engineering for anthocyanin production. Anthocyanins (ranging from red to blue) are...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1007/s11103-018-0771-4
更新日期：2018-09-01 00:00:00
abstract：:The R and B genes of maize regulate the anthocyanin biosynthetic pathway and constitute a small gene family whose evolution has been shaped by polyploidization and transposable element activity. To compare the evolution of regulatory genes in the distinct but related genomes of rice and maize, we previously isolated t...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006355510883
更新日期：2000-03-01 00:00:00
abstract：:Plants respond to phosphate (Pi) deficiency through adaptive mechanisms involving several morphological, biochemical and molecular changes. In this study, we have characterized the structure and expression of a tomato (Lycopersicon esculentum L.) phosphate starvation-induced gene (TPSI1). A 3.5 kb genomic fragment con...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005729309569
更新日期：1997-03-01 00:00:00
abstract：KEY MESSAGE:HbMBF1a was isolated and characterized in H. brevisubulatum, and overexpressed HbMBF1a could enhance the salt tolerance and ABA insensitivity in Arabidopsis thaliana. The transcript levels of stress-responsive genes were significantly increased in the transgenic lines under salt and ABA conditions. Salinity...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-019-00926-7
更新日期：2020-01-01 00:00:00
abstract：:Polyploidization has repeatedly occurred during plant evolution. Although autopolyploidy is the best model to characterize the polyploidization effects in a highly controlled manner, there are limited studies on autopolyploids compared to allopolyploids. To improve our understanding of autopolyploidy effects in maize,...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-010-9722-4
更新日期：2011-02-01 00:00:00
abstract：:The mitochondrial DNA molecules of two interfertile algal species, Chlamydomonas smithii and C. reinhardtii, are co-linear except for a 1075 bp intron (the alpha-insert) that is present in the cob gene of C. smithii. The alpha-insert, a group I intron (Cs cob.1) containing an open reading frame (ORF) which encodes a b...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019218
更新日期：1992-03-01 00:00:00
abstract：:Chloroplast and cytosolic triosephosphate isomerases from spinach were separated and purified to homogeneity. Both enzymes were partially sequenced by Edman degradation. Using degenerate primers designed against the amino acid sequences, a homologous probe for the chloroplast enzyme was amplified and used to isolate s...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019506
更新日期：1994-12-01 00:00:00
abstract：KEY MESSAGE:Lack of structural components results in inflorescence stem bending. Differentially expressed genes involved in lignin and hemicellulose biosynthesis are vital; genes involved in cellulose and glycan biosynthesis are also relevant. An erect inflorescence stem is essential for high-quality cut herbaceous peo...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-019-00945-4
更新日期：2020-02-01 00:00:00
abstract：:RNA silencing is a collective term that encompasses the sequence of events that leads to the targeted degradation of cellular mRNA and thus to the silencing of corresponding gene expression. RNA silencing is initiated after introduction into the host genome of a gene that is homologous to an endogenous gene. Transcrip...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1023/B:PLAN.0000028797.63168.a7
更新日期：2004-01-01 00:00:00
abstract：:A seedling lethal mutant of Nicotiana plumbaginifolia (sdl-1) was isolated by transposon tagging using a maize Dissociation (Ds) element. The insertion mutation was produced by direct co-transformation of protoplasts with two plasmids: one containing Ds and a second with an Ac transposase gene. sdl-1 seedlings exhibit...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1019851913083
更新日期：2002-10-01 00:00:00
abstract：KEY MESSAGE:We identified and cloned the two precursors of miR158 and its target gene in Brassica campestris ssp. chinensis, which both had high relative expression in the inflorescences. Further study revealed that over-expression of miR158 caused reduced pollen varbility, which was caused by the degradation of pollen...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0563-7
更新日期：2017-02-01 00:00:00
abstract：:The function of the secretory pathway is dependent on multiple protein-protein interactions at various stages. Currently, such interactions are mainly studied using physical methods that document direct contact or affinity in vitro. The development of vital fluorescence imaging as well as quantitative protein transpor...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1023/a:1021266320877
更新日期：2002-12-01 00:00:00
abstract：:Cab-1 is a complex genetic locus in tomato consisting of four clustered genes encoding chlorophyll a/b-binding polypeptide. Southern blot analysis of total tomato DNA with genomic clones corresponding to the Cab-1 locus has revealed the presence of a repetitive element in the 3 kb spacer regions between two of these g...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00014948
更新日期：1988-09-01 00:00:00
abstract：:Persian walnut (Juglans regia L.) is an economically important tree for its nut crop and timber. To gain insight into the structure and evolution of the walnut genome, we constructed two bacterial artificial chromosome (BAC) libraries, containing a total of 129,024 clones, from in vitro-grown shoots of J. regia cv. Ch...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-011-9849-y
更新日期：2012-01-01 00:00:00
abstract：:The soluble acyl-ACP:sn-glycerol-3-phosphate acyltransferase from chloroplasts of chilling-sensitive and -resistant plants differ in their fatty acid selectivity. Enzymes from resistant plants discriminate against non-fluid palmitic acid and select oleic acid whereas the acyltransferase from sensitive plants accepts b...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00037145
更新日期：1991-11-01 00:00:00
abstract：:The multiple copies of the chloroplast genome (plastome) are condensed and organized into nucleoids by a set of proteins. One of these, the DNA-binding protein DCP68 from soybean, has previously been shown to compact DNA and to inhibit DNA synthesis in vitro. N-terminal amino acid analysis and the absorption spectrum ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1015500431421
更新日期：2002-08-01 00:00:00
abstract：KEY MESSAGE:Leveraging the heightened levels of polymorphism in NB-ARC-related protein encoding genes in higher plants, a bioinformatic pipeline was created to identify regions in this gene family from sequenced plant genomes that exhibit fragment length or single nucleotide differences in different accessions of the s...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-018-0774-1
更新日期：2018-10-01 00:00:00
abstract：:To analyse experimentally the correlation between transgene silencing and the presence of an inverted repeat in transgenic Arabidopsis thaliana plants, expression of the beta-glucuronidase (gus) gene was studied when present as a convergently transcribed inverted repeat or as a single copy in otherwise isogenic lines....
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1010614522706
更新日期：2001-07-01 00:00:00
abstract：:The first step in the routing of newly synthesized proteins into the secretory pathway is the binding of the nascent signal sequence to the signal recognition particle. The mammalian signal recognition particle is a complex consisting of 6 proteins and a single 7S RNA molecule. Signal recognition particle-like complex...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00042359
更新日期：1993-12-01 00:00:00
