Abstract:
:Higher plant microspores, when subjected to various stress treatments in vitro, are able to reprogram their regular gametophytic development towards the sporophytic pathway to form haploid embryos and plants. Suppression subtractive hybridization (SSH) and metabolic profiling were used to characterize this developmental switch. Following differential reverse Northern hybridizations 90 distinct up-regulated sequences were identified in stressed, embryogenic microspores (accessible at www.univie.ac.at/ntsm). Sequence analyses allowed the classification of these genes into functional clusters such as metabolism, chromosome remodeling, signaling, transcription and translation, while the putative functions of half of the sequences remained unknown. A comparison of metabolic profiles of non-stressed and stressed microspores using gas chromatography/mass spectrometry (GC/MS) identified 70 compounds, partly displaying significant changes in metabolite levels, e.g., highly elevated levels of isocitrate and isomaltose in stressed microspores compared to non-stressed microspores. The formation of embryogenic microspores is discussed on the basis of the identified transcriptional and metabolic profiles.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Hosp J,Tashpulatov A,Roessner U,Barsova E,Katholnigg H,Steinborn R,Melikant B,Lukyanov S,Heberle-Bors E,Touraev Adoi
10.1007/s11103-006-9078-ysubject
Has Abstractpub_date
2007-01-01 00:00:00pages
137-49issue
1eissn
0167-4412issn
1573-5028journal_volume
63pub_type
杂志文章abstract::Due to an unfortunate turn of events, the funding note for Open Access publication was not properly provided in the original publication. Hence, the original article has been corrected. The opening line of the Acknowledgement section should read. ...
journal_title:Plant molecular biology
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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