Transcriptional and metabolic profiles of stress-induced, embryogenic tobacco microspores.

Abstract:

:Higher plant microspores, when subjected to various stress treatments in vitro, are able to reprogram their regular gametophytic development towards the sporophytic pathway to form haploid embryos and plants. Suppression subtractive hybridization (SSH) and metabolic profiling were used to characterize this developmental switch. Following differential reverse Northern hybridizations 90 distinct up-regulated sequences were identified in stressed, embryogenic microspores (accessible at www.univie.ac.at/ntsm). Sequence analyses allowed the classification of these genes into functional clusters such as metabolism, chromosome remodeling, signaling, transcription and translation, while the putative functions of half of the sequences remained unknown. A comparison of metabolic profiles of non-stressed and stressed microspores using gas chromatography/mass spectrometry (GC/MS) identified 70 compounds, partly displaying significant changes in metabolite levels, e.g., highly elevated levels of isocitrate and isomaltose in stressed microspores compared to non-stressed microspores. The formation of embryogenic microspores is discussed on the basis of the identified transcriptional and metabolic profiles.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Hosp J,Tashpulatov A,Roessner U,Barsova E,Katholnigg H,Steinborn R,Melikant B,Lukyanov S,Heberle-Bors E,Touraev A

doi

10.1007/s11103-006-9078-y

subject

Has Abstract

pub_date

2007-01-01 00:00:00

pages

137-49

issue

1

eissn

0167-4412

issn

1573-5028

journal_volume

63

pub_type

杂志文章
  • Correction to: CYP79 P450 monooxygenases in gymnosperms: CYP79A118 is associated with the formation of taxiphyllin in Taxus baccata.

    abstract::Due to an unfortunate turn of events, the funding note for Open Access publication was not properly provided in the original publication. Hence, the original article has been corrected. The opening line of the Acknowledgement section should read. ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,已发布勘误

    doi:10.1007/s11103-017-0674-9

    authors: Luck K,Jia Q,Huber M,Handrick V,Wong GK,Nelson DR,Chen F,Gershenzon J,Köllner TG

    更新日期:2017-12-01 00:00:00

  • The molecular and biochemical basis for varietal variation in sesquiterpene content in melon (Cucumis melo L.) rinds.

    abstract::A combined chemical, biochemical and molecular study was conducted to understand the differential accumulation of volatile sesquiterpenes in melon fruits. Sesquiterpenes were present mainly in the rinds of climacteric varieties, and a great diversity in their composition was found among varieties. Sesquiterpenes were ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-008-9296-6

    authors: Portnoy V,Benyamini Y,Bar E,Harel-Beja R,Gepstein S,Giovannoni JJ,Schaffer AA,Burger J,Tadmor Y,Lewinsohn E,Katzir N

    更新日期:2008-04-01 00:00:00

  • The two genes for the small subunit of RuBP Carboxylase/oxygenase are closely linked in Chlamydomonas reinhardtii.

    abstract::Ribulose bisphosphate carboxylase-oxygenase (Rubisco) is a key enzyme in the photosynthetic fixation of CO2 by the chloroplast. The synthesis of the enzyme is an example of the cooperation between the chloroplast and the nucleocytoplasmic compartments, as it is assembled from subunits encoded in the two respective gen...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00021302

    authors: Goldschmidt-Clermont M

    更新日期:1986-01-01 00:00:00

  • Leaf initiation: the integration of growth and cell division.

    abstract::The shoot apical meristem of higher plants is characterized by a conserved pattern of cell division, the functional significance of which is unclear. Although a causal role for cell division frequency and orientation in morphogenesis has been suggested, supporting data are limited. An alternative interpretation laying...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审

    doi:10.1007/s11103-005-7703-9

    authors: Fleming AJ

    更新日期:2006-04-01 00:00:00

  • Immunological evidence for accumulation of two high-molecular-weight (104 and 90 kDa) HSPs in response to different stresses in rice and in response to high temperature stress in diverse plant genera.

    abstract::Rice seedlings accumulate stainable amounts of the 104 and 90 kDa polypeptides in response to high temperature stress. We have purified and raised highly specific polyclonal antisera against both of these polypeptides. In western blotting experiments, we find that these proteins are accumulated to different extents in...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00043653

    authors: Pareek A,Singla SL,Grover A

    更新日期:1995-10-01 00:00:00

  • Molecular cloning of two tandemly arranged peroxidase genes from Populus kitakamiensis and their differential regulation in the stem.

    abstract::A genomic library was prepared from Populus kitakamiensis and screened with the cDNA for an anionic peroxidase from P. kitakamiensis. One genomic clone was isolated that contained two tandemly oriented genes for anionic peroxidases, prxA3a and prxA4a. Both genes consisted of four exons and three introns; the introns h...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00021193

    authors: Osakabe K,Koyama H,Kawai S,Katayama Y,Morohoshi N

    更新日期:1995-07-01 00:00:00

  • Carotenoid deficiency impairs ABA and IAA biosynthesis and differentially affects drought and cold tolerance in rice.

    abstract::Plant responses to abiotic stresses are coordinated by arrays of growth and developmental programs. Phytohormones such as abscisic acid (ABA) and indole-3-acetic acid (IAA) play critical roles in developmental progresses and environmental responses through complex signalling networks. However, crosstalk between the tw...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-013-0103-7

    authors: Du H,Wu N,Chang Y,Li X,Xiao J,Xiong L

    更新日期:2013-11-01 00:00:00

  • An OGG1 orthologue encoding a functional 8-oxoguanine DNA glycosylase/lyase in Arabidopsis thaliana.

    abstract::Repair of the ubiquitous mutagenic lesion 7,8-dihydro-8-oxoguanine (8-oxoG) is initiated in eukaryotes by DNA glycosylases/lyases, such as yeast Ogg1, that do not share significant sequence identity with their prokaryotic counterparts, typified by Escherichia coli MutM (Fpg) protein. The unexpected presence of a funct...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1013644026132

    authors: García-Ortiz MV,Ariza RR,Roldán-Arjona T

    更新日期:2001-12-01 00:00:00

  • Localization and nucleotide sequence of the gene for the membrane polypeptide D2 from pea chloroplast DNA.

    abstract::The gene for the membrane polypeptide D2 has been mapped on the pea (Pisum sativum) chloroplast genome. The nucleotide sequence of the gene and its flanking regions is presented. The only large open reading frame in the sequence codes for a protein of MW 39.5 kD. A potential ribosome binding site is located 6 nucleoti...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00029654

    authors: Rasmussen OF,Bookjans G,Stummann BM,Henningsen KW

    更新日期:1984-07-01 00:00:00

  • Cloning of two gibberellin-regulated cDNAs from Arabidopsis thaliana by subtractive hybridization: expression of the tonoplast water channel, gamma-TIP, is increased by GA3.

    abstract::The Arabidopsis ga1 mutant has very low levels of endogenous, active gibberellins and thus has an extreme dwarf phenotype; application of GA3 induces stem elongation and flower development. To test the hypothesis that GA action in this system involves changes in gene expression, we have cloned mRNAs whose abundance ch...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00023557

    authors: Phillips AL,Huttly AK

    更新日期:1994-02-01 00:00:00

  • Maintenance DNA methyltransferase (Met1) and silencing of CpG-methylated foreign DNA in Volvox carteri.

    abstract::DNA methylation plays an important role in the gene-silencing network of higher eukaryotes. We have analyzed the 21.5-kb maintenance methyltransferase (M-MTase) gene, met1, of the multicellular green alga Volvox carteri. The met1 transcript was detected only during the period when DNA replication and cell division are...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-006-9091-1

    authors: Babinger P,Völkl R,Cakstina I,Maftei A,Schmitt R

    更新日期:2007-02-01 00:00:00

  • Developmental regulation of the PsbS gene expression in spinach seedlings: the role of phytochrome.

    abstract::The PsbS gene product (PSII-S) which is an integral subunit of photosystem II has recently been reported to be a new type of pigment-binding protein [11]. The chlorophylls of the PSII-S protein exhibit weak excitonic coupling and this protein is stable also in the absence of pigments. Here we investigated the expressi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00019467

    authors: Adamska I,Funk C,Renger G,Andersson B

    更新日期:1996-07-01 00:00:00

  • Identification of two alfalfa early nodulin genes with homology to members of the pea Enod12 gene family.

    abstract::In a search for plant genes expressed during early symbiotic interactions between Medicago sativa and Rhizobium meliloti, we have isolated and characterized two alfalfa genes which have strong sequence similarity to members of the Enod12 gene family of Pisum sativum. The M. sativa genes, MsEnod12A and B, encode putati...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00019952

    authors: Allison LA,Kiss GB,Bauer P,Poiret M,Pierre M,Savouré A,Kondorosi E,Kondorosi A

    更新日期:1993-01-01 00:00:00

  • FLEXIBLE CULM 1 encoding a cinnamyl-alcohol dehydrogenase controls culm mechanical strength in rice.

    abstract::Culm mechanical strength is an important agronomic trait in crop breeding. To understand the molecular mechanisms that control culm mechanical strength, we identified a flexible culm1 (fc1) mutant by screening a rice T-DNA insertion mutant library. This mutant exhibited an abnormal development phenotype, including lat...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-008-9448-8

    authors: Li X,Yang Y,Yao J,Chen G,Li X,Zhang Q,Wu C

    更新日期:2009-04-01 00:00:00

  • Determination of copy number and linkage relationships among five actin gene subfamilies in Petunia hybrida.

    abstract::The actin gene superfamily of Petunia hybrida cv. Mitchell contains greater than 100 gene members which have been divided into several highly divergent subfamilies [1]. Five subfamily-specific probes have been used to compare the actin genes among the Mitchell, Violet 23 (V23) and Red 51 (R51) cultivars of P. hybrida....

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00017466

    authors: McLean M,Baird WV,Gerats AG,Meagher RB

    更新日期:1988-09-01 00:00:00

  • Insect resistance of transgenic plants that express modified Bacillus thuringiensis cryIA(b) and cryIC genes: a resistance management strategy.

    abstract::Tobacco and tomato plants were generated exhibiting insect resistance due to the introduction of modified cryIA(b) and cryIC genes of Bacillus thuringiensis. Limited modifications at selected regions of the coding sequences of both genes are sufficient to obtain resistance against Spodoptera exigua, Heliothis virescen...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00039519

    authors: van der Salm T,Bosch D,Honée G,Feng L,Munsterman E,Bakker P,Stiekema WJ,Visser B

    更新日期:1994-10-01 00:00:00

  • Normal and lysine-containing zeins are unstable in transgenic tobacco seeds.

    abstract::Chimeric genes composed of the beta-phaseolin promoter, an alpha-zein coding sequence and its modified versions containing lysine codons, and a beta-zein polyadenylation signal were inserted into the genome of tobacco by Agrobacterium-mediated transformation. alpha-Zein mRNA levels in the transgenic tobacco seeds 20 d...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00017922

    authors: Ohtani T,Galili G,Wallace JC,Thompson GA,Larkins BA

    更新日期:1991-01-01 00:00:00

  • Tobacco microtubule-associated protein, MAP65-1c, bundles and stabilizes microtubules.

    abstract::Three genes that encode MAP65-1 family proteins have been identified in the Nicotiana tabacum genome. In this study, NtMAP65-1c fusion protein was shown to bind and bundle microtubules (MTs). Further in vitro investigations demonstrated that NtMAP65-1c not only alters MT assembly and nucleation, but also exhibits high...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-010-9694-4

    authors: Meng Q,Du J,Li J,Lü X,Zeng X,Yuan M,Mao T

    更新日期:2010-12-01 00:00:00

  • Cloning and characterization of a second form of the rice adenine phosphoribosyl transferase gene (OsAPT2) and its association with TGMS.

    abstract::A rice gene, OsAPT2, which encodes a putative adenine phosphoribosyl transferase (APRT), was cloned and characterized. Analysis of the cDNA and genomic sequences revealed seven exons and six introns in the OsAPT2. The deduced amino acid sequence of OsAPT2 is highly homologous to those of previously isolated APRTs. RT-...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-005-4208-5

    authors: Zhou CJ,Li J,Zou JC,Liang FS,Ye CJ,Jin DM,Weng ML,Wang B

    更新日期:2006-02-01 00:00:00

  • HLMethy: a machine learning-based model to identify the hidden labels of m6A candidates.

    abstract:KEY MESSAGE:We developed a machine learning-based model to identify the hidden labels of m6A candidates from noisy m6A-seq data. Peak-calling approaches, such as MeRIP-seq or m6A-seq, are commonly used to map m6A modifications. However, these technologies can only map m6A sites with 100-200 nt resolution and cannot rev...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-019-00930-x

    authors: Liu Z,Dong W,Luo W,Jiang W,Li Q,He Z

    更新日期:2019-12-01 00:00:00

  • Ectopic expression of the Arabidopsis MINI ZINC FINGER1 and MIF3 genes induces shoot meristems on leaf margins.

    abstract::A leaf undergoes determinate growth from a primordium on flank of the shoot apical meristem. Several intrinsic pathways restrict meristematic activity in the leaf of Arabidopsis; however, other factors remain to be defined. We report here that the overexpression of MINI ZINC FINGER1 (MIF1) or MIF3 disrupted the leaf d...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-011-9768-y

    authors: Hu W,Feng B,Ma H

    更新日期:2011-05-01 00:00:00

  • Expression of the Pib rice-blast-resistance gene family is up-regulated by environmental conditions favouring infection and by chemical signals that trigger secondary plant defences.

    abstract::The rice blast resistance gene Pib is a member of the nucleotide binding site (NBS) and leucine-rich repeat (LRR) class of plant disease resistance (R) genes and belongs to a small gene family. We describe here the isolation and characterization of a Pib homologue (PibH8), and extensive investigation of the expression...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1012457113700

    authors: Wang ZX,Yamanouchi U,Katayose Y,Sasaki T,Yano M

    更新日期:2001-11-01 00:00:00

  • The peach HECATE3-like gene FLESHY plays a double role during fruit development.

    abstract::Tight control of cell/tissue identity is essential for a correct and functional organ patterning, an important component of overall fruit development and eventual maturation and ripening. Despite many investigations regarding the molecular determinants of cell identity in fruits of different species, a useful model ab...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-016-0445-z

    authors: Botton A,Rasori A,Ziliotto F,Moing A,Maucourt M,Bernillon S,Deborde C,Petterle A,Varotto S,Bonghi C

    更新日期:2016-05-01 00:00:00

  • New features of desiccation tolerance in the lichen photobiont Trebouxia gelatinosa are revealed by a transcriptomic approach.

    abstract::Trebouxia is the most common lichen-forming genus of aero-terrestrial green algae and all its species are desiccation tolerant (DT). The molecular bases of this remarkable adaptation are, however, still largely unknown. We applied a transcriptomic approach to a common member of the genus, T. gelatinosa, to investigate...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-016-0468-5

    authors: Carniel FC,Gerdol M,Montagner A,Banchi E,De Moro G,Manfrin C,Muggia L,Pallavicini A,Tretiach M

    更新日期:2016-06-01 00:00:00

  • NtERF32: a non-NIC2 locus AP2/ERF transcription factor required in jasmonate-inducible nicotine biosynthesis in tobacco.

    abstract::Nicotine biosynthesis in tobacco (Nicotiana tabacum L.) is highly regulated by jasmonic acid (JA). Two nuclear loci, A and B (renamed NIC1 and NIC2) have been identified that mediate JA-inducible nicotine formation and total alkaloid accumulation. NIC2 was recently shown to be a cluster of seven genes encoding Apetala...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-013-0116-2

    authors: Sears MT,Zhang H,Rushton PJ,Wu M,Han S,Spano AJ,Timko MP

    更新日期:2014-01-01 00:00:00

  • Serial analysis of gene expression (SAGE) of host-plant resistance to the cassava mosaic disease (CMD).

    abstract::Cassava mosaic disease (CMD) is a viral disease of the important tropical staple crop cassava (Manihot esculenta) and preferred management involves use of host-plant resistance. The best available resistance is controlled by a single dominant gene. Serial analysis of gene expression (SAGE) was used to analyze the gene...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-004-3477-8

    authors: Fregene M,Matsumura H,Akano A,Dixon A,Terauchi R

    更新日期:2004-11-01 00:00:00

  • A novel proline-rich protein from wheat.

    abstract::A cDNA (WPRP1) encoding a wheat proline-rich protein has been isolated and sequenced. The amino acid composition shows 45% proline, with high levels of methionine, lysine and glutamic acid. The derived 378 residue amino acid sequence has a highly repetitive structure which is unlike those of other proline-rich protein...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00023430

    authors: Raines CA,Lloyd JC,Chao SM,John UP,Murphy GJ

    更新日期:1991-04-01 00:00:00

  • Transcriptomic adaptations in rice suspension cells under sucrose starvation.

    abstract::Sugar is an important resource for energy generation and developmental regulation in plants, and sucrose starvation causes enormous changes in cellular morphology, enzyme activities and gene expression. Genome-wide gene expression profiling provides a comprehensive knowledge of gene expression under nutrient depletion...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-006-9100-4

    authors: Wang HJ,Wan AR,Hsu CM,Lee KW,Yu SM,Jauh GY

    更新日期:2007-03-01 00:00:00

  • Site-directed mutagenesis of a tetrameric dandelion polyphenol oxidase (PPO-6) reveals the site of subunit interaction.

    abstract::Polyphenol oxidases (PPOs) catalyze the oxidation of ortho-diphenols to the corresponding quinones (EC 1.10.3.1). In plants PPOs appear in gene families, and the corresponding isoenzymes are located to the thylakoid lumen of chloroplasts. Although plant PPOs are often discussed with regard to their role in defense rea...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-012-9943-9

    authors: Dirks-Hofmeister ME,Inlow JK,Moerschbacher BM

    更新日期:2012-09-01 00:00:00

  • Characterization and comparison of intron structure and alternative splicing between Medicago truncatula, Populus trichocarpa, Arabidopsis and rice.

    abstract::Alignment of transcripts and genome sequences yielded a set of alternatively spliced transcripts in four angiosperm genomes: three dicotyledon species Medicago truncatula (Medicago), Populus trichocarpa (poplar) and Arabidopsis thaliana (Arabidopsis), and the monocotyledon Oryzae sativa (rice). Intron retention was th...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-008-9334-4

    authors: Baek JM,Han P,Iandolino A,Cook DR

    更新日期:2008-07-01 00:00:00