Abstract：
:Chimeric genes composed of the beta-phaseolin promoter, an alpha-zein coding sequence and its modified versions containing lysine codons, and a beta-zein polyadenylation signal were inserted into the genome of tobacco by Agrobacterium-mediated transformation. alpha-Zein mRNA levels in the transgenic tobacco seeds 20 days after self-pollination varied between 1.0% and 2.5% of the total mRNA population. At 25 days after pollination the 19 kDa alpha-zein was immunologically detected with a polyclonal antiserum in protein extracts from the seeds of transgenic plants. The transgenic plant with the highest level of zein gene expression had an alpha-zein content that was approximately 0.003% of the total seed protein. The amount of alpha-zein in other transgenic plants varied between 1 x 10(-4)% and 1 x 10(-5)% of the total seed protein. The differences in the amounts of mRNA and protein did not correlate with the lysine substitutions introduced into the alpha-zein protein. Polysomes translating alpha-zein mRNA isolated from tobacco seeds contained fever ribosomes than those from maize endosperm, but this did not appear to be the cause of the inefficient protein synthesis. In vivo labelling and immunoprecipitation indicated that newly synthesized alpha-zein was degraded in tobacco seeds with a half-life of less than 1 hour.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Ohtani T,Galili G,Wallace JC,Thompson GA,Larkins BAdoi
10.1007/BF00017922subject
Has Abstractpub_date
1991-01-01 00:00:00pages
117-28issue
1eissn
0167-4412issn
1573-5028journal_volume
16pub_type
杂志文章abstract：:Deepwater rice can grow in the regions of Southeast Asia that are flooded during the monsoon season because it has several adaptations allowing it to survive under flooded conditions. One such adaptation is the ability for rapid internode elongation upon partial submergence to maintain its foliage above the rising flo...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/b:plan.0000009693.26740.c3
更新日期：1997-01-01 00:00:00
abstract：:Polyploidization has repeatedly occurred during plant evolution. Although autopolyploidy is the best model to characterize the polyploidization effects in a highly controlled manner, there are limited studies on autopolyploids compared to allopolyploids. To improve our understanding of autopolyploidy effects in maize,...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-010-9722-4
更新日期：2011-02-01 00:00:00
abstract：:Rice seedlings accumulate stainable amounts of the 104 and 90 kDa polypeptides in response to high temperature stress. We have purified and raised highly specific polyclonal antisera against both of these polypeptides. In western blotting experiments, we find that these proteins are accumulated to different extents in...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00043653
更新日期：1995-10-01 00:00:00
abstract：:In yeast, Rad21/Scc1 and its meiotic variant Rec8 are key players in the establishment and subsequent dissolution of sister chromatid cohesion for mitosis and meiosis, respectively, which are essential for chromosome segregation. Unlike yeast, our identification revealed that the rice genome has 4 RAD21-like genes tha...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-005-4922-z
更新日期：2006-03-01 00:00:00
abstract：:In order to optimise expression of a foreign protein in transgenic plants we investigated the potential benefits of including a viral untranslated leader sequence within a plant transformation vector. A variety of 5 leaders, including the tobacco mosaic virus (TMV) leader sequence and 31 nucleotides of the cauliflower...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00021423
更新日期：1993-10-01 00:00:00
abstract：:In this paper we present the structural analysis of the 1-aminocyclopropane-1-carboxylate (ACC) oxidase gene family from Petunia hybrida. Southern blot analysis and restriction endonuclease mapping showed that two cloned regions of the petunia genome contained sequences highly homologous to a previously isolated ACC o...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00042349
更新日期：1993-12-01 00:00:00
abstract：:The amounts of a 1.9 kb mitochondrial plasmid relative to sequences in another mitochondrial DNA replicon and also to nuclear ribosomal DNA sequences have been compared in maize leaves and anthers. Similar comparisons have been made between plants with the same nuclear genotype but containing normal, S, or T cytoplasm...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF02418241
更新日期：1985-07-01 00:00:00
abstract：:The complex capitulum of Chrysanthemum morifolium is often comprised of bilaterally symmetrical ray florets and radially symmetrical disc florets. The TCP transcription factor clade CYCLOIDEA2 (CYC2) appears to play a vital role in determining floral symmetry and in regulating floral organ development in Asteraceae. O...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-020-00981-5
更新日期：2020-05-01 00:00:00
abstract：:The PR-like proteins, class I beta-1,3-glucanase (GLU I) and chitinase (CHN I), are induced as part of a stereotypic response that can provide protection against viral, bacterial, and fungal pathogens. We have identified two Nicotiana plumbaginifolia ankyrin-repeat proteins, designated Glucanohydrolase Binding Protein...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-004-4679-9
更新日期：2004-11-01 00:00:00
abstract：:GRAS proteins belong to a plant-specific transcription factor family. Currently, 33 GRAS members including a putative expressed pseudogene have been identified in the Arabidopsis genome. With a reverse genetic approach, we have constructed a "phenome-ready unimutant collection" of the GRAS genes in Arabidopsis thalian...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9345-1
更新日期：2008-08-01 00:00:00
abstract：:We have identified two single-copy genes from the model legume. Medicago truncatula (MtENOD16 and 20) whose expression can be correlated with early stages of root nodulation and whose predicted coding sequences are partially homologous to both pea/vetch ENOD5 and soybean N315/ENOD55. Database searching and sequence al...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005916821224
更新日期：1998-03-01 00:00:00
abstract：:Infection of tobacco by tobacco mosaic virus (TMV) induces coordinate expression of genes encoding acidic and basic beta-1,3-glucanase isoforms. These genes are differentially expressed in response to other treatments. Salicylate treatment induces acidic glucanase mRNA to a higher level than basic glucanase mRNA. Ethy...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00028803
更新日期：1993-02-01 00:00:00
abstract：:The beneficial root-colonizing fungus Piriformospora indica stimulates root development of Chinese cabbage (Brassica campestris subsp. Chinensis) and this is accompanied by the up-regulation of a τ-class glutathione (GSH)-S-transferase gene (BcGSTU) (Lee et al. 2011) in the roots. BcGSTU expression is further promoted...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0531-2
更新日期：2016-12-01 00:00:00
abstract：:DNA methylation plays an important role in the gene-silencing network of higher eukaryotes. We have analyzed the 21.5-kb maintenance methyltransferase (M-MTase) gene, met1, of the multicellular green alga Volvox carteri. The met1 transcript was detected only during the period when DNA replication and cell division are...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-006-9091-1
更新日期：2007-02-01 00:00:00
abstract：:Glucosinolates are a class of thioglycosides found predominantly in plants of the order Brassicales whose function in anti-herbivore defense has been attributed to the products formed by myrosinase-catalyzed hydrolysis upon plant tissue damage. The most common type of hydrolysis products, the isothiocyanates, are toxi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-006-9071-5
更新日期：2007-01-01 00:00:00
abstract：:Hevea brasiliensis, being the only source of commercial natural rubber, is an extremely economically important crop. In an effort to facilitate biological, biochemical and molecular research in rubber biosynthesis, here we report the use of next-generation massively parallel sequencing technologies and de novo transcr...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-011-9811-z
更新日期：2011-10-01 00:00:00
abstract：:Screening of a lambda gt11 cDNA expression library of Euglena gracilis with antibodies directed against histones H2 from maize resulted in the isolation of a full-length cDNA for a histone H2A. The open-reading frame of 408 bp corresponded to a protein of 136 amino acid residues (14 kDa). Despite the presence of a pol...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00014447
更新日期：1994-03-01 00:00:00
abstract：:We have previously shown that two CRE elements situated on a 31 bp region of the cauliflower mosaic virus (CaMV) 35S promoter activate gene expression in the yeast Saccharomyces cerevisiae and are regulated by cAMP. Studies with the yeast transcription factors GCN4, SKO1 and YAP1, which bind CRE-like sequences, showed...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00023248
更新日期：1994-05-01 00:00:00
abstract：:The root knot nematode resistance gene Mi in tomato has been mapped in the pericentromeric region of chromosome 6. With the objective of isolating Mi through a map-based cloning approach, we have previously identified and ordered into a high-resolution genetic linkage map a variety of tightly linked molecular markers....
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00021430
更新日期：1993-10-01 00:00:00
abstract：:A leaf cDNA library from hybrid aspen, Populus tremula x tremuloides, was constructed. From this two different cDNA clones, denoted CA1a and CA1b, encoding a chloroplastic carbonic anhydrase (CA) were isolated and DNA sequenced. Analysis of the deduced amino acid sequences showed that the isolated CAs belong to the be...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005849202731
更新日期：1997-07-01 00:00:00
abstract：:A hybrid Cauliflower Mosaic Virus (CaMV) genome containing a selectable marker gene was constructed by replacing the gene VI coding region with the aminoglycoside (neomycin) phosphotransferase type II [APH(3')II] gene from Tn5. This modified viral genome was tested for its infectivity both in planta and in a protoplas...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00034937
更新日期：1986-09-01 00:00:00
abstract：:The LI818 gene from Chlamydomonas encodes a polypeptide that is related to the chlorophyll a/b-binding proteins (CAB) of higher plants and green algae. However, despite this relatedness, LI818 gene expression is not coordinated with that of cab genes and is regulated differently by light, suggesting a different role f...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006340308077
更新日期：2000-01-01 00:00:00
abstract：:The nopaline synthase (nos) promoter is expressed in a wide range of plant cell types and regulated by various developmental and environmental factors. The nos upstream control region essential for this regulation was studied by means of synthetic oligomers using transient and stable transformation systems. Insertion ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040578
更新日期：1994-01-01 00:00:00
abstract：:Drought is one of the critical factors limiting reproductive yields of rice and other crops globally. However, little is known about the molecular mechanism underlying reproductive development under drought stress in rice. To explore the potential gene function for improving rice reproductive development under drought...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-013-0057-9
更新日期：2013-06-01 00:00:00
abstract：:Repair of the ubiquitous mutagenic lesion 7,8-dihydro-8-oxoguanine (8-oxoG) is initiated in eukaryotes by DNA glycosylases/lyases, such as yeast Ogg1, that do not share significant sequence identity with their prokaryotic counterparts, typified by Escherichia coli MutM (Fpg) protein. The unexpected presence of a funct...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1013644026132
更新日期：2001-12-01 00:00:00
abstract：:Arabidopsis accessions are either winter-annuals, which require cold winter temperatures for spring flowering, or rapid-cycling summer annuals. Typically, winter annual accessions have functional FRIGIDA (FRI) and FRIGIDA-LIKE 1 (FRL1) proteins that promote high expression of FLOWERING LOCUS C (FLC), which prevents fl...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-010-9635-2
更新日期：2010-07-01 00:00:00
abstract：:We describe here the isolation and characterization of OsiSAP8, a member of stress Associated protein (SAP) gene family from rice characterized by the presence of A20 and AN1 type Zinc finger domains. OsiSAP8 is a multiple stress inducible gene, induced by various stresses, namely heat, cold, salt, desiccation, submer...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-007-9284-2
更新日期：2008-03-01 00:00:00
abstract：:Plant cell suspension cultures are invaluable models for the study of cellular processes. Here we develop the recently described Arabidopsis suspension culture MM2d as a transcript profiling platform by means of Affymetrix ATH1 microarrays. Analysis of gene expression profiles during normal culture growth, during sync...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/B:PLAN.0000019059.56489.ca
更新日期：2003-11-01 00:00:00
abstract：:APETALA2 (AP2) transcription factors (TFs) play very important roles in plant growth and development and in defense response. Here, we report functional characterization of four AP2 TF family genes [(RAP2.6 (At1g43160), RAP2.6L (At5g13330), DREB 26 (At1g21910) and DREB19 (At2g38340)] that were identified among NaCl in...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-010-9711-7
更新日期：2011-01-01 00:00:00
abstract：:Arabinogalactan-proteins (AGPs) are a family of complex proteoglycans found in all higher plants. Although the precise function(s) of any single AGP is unknown, they are implicated in diverse developmental roles such as differentiation, cell-cell recognition, embryogenesis and programmed cell death. DNA sequencing pro...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：
更新日期：2001-09-01 00:00:00
