Abstract:
:A leaf cDNA library from hybrid aspen, Populus tremula x tremuloides, was constructed. From this two different cDNA clones, denoted CA1a and CA1b, encoding a chloroplastic carbonic anhydrase (CA) were isolated and DNA sequenced. Analysis of the deduced amino acid sequences showed that the isolated CAs belong to the beta-CA family, and have identities around 70% to other dicotyledonous plant CAs. The two hybrid aspen cDNA clones display a high nucleotide sequence identity, only 12 nucleotides differ. Since only one gene copy of this soluble chloroplastic CA is present in the nuclear genome, we postulate that the two isolated cDNA clones are alleles. Northern blot hybridization revealed a CA transcript of ca. 1300 bases, 140 bases shorter than in pea. Western and northern blot hybridizations on crude protein extracts and on total RNA, respectively, isolated from stem and leaves, showed that hybrid aspen CA is expressed specifically in the leaf under the growth conditions used. Based on the deduced amino acid sequence, the mature hybrid aspen CA enzyme subunit has a molecular mass of 24.8 kDa. The enzyme was over-expressed in Escherichia coli, and purified by affinity chromatography. Biochemical characterization showed that the protein structure and the CO2-hydration activity are similar to the pea enzyme. Molecular characterization of a CA from a perennial plant has not previously been performed, and it demonstrates that both the structure and activity of hybrid aspen CA resembles CAs from annual plants.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Larsson S,Björkbacka H,Forsman C,Samuelsson G,Olsson Odoi
10.1023/a:1005849202731subject
Has Abstractpub_date
1997-07-01 00:00:00pages
583-92issue
4eissn
0167-4412issn
1573-5028journal_volume
34pub_type
杂志文章abstract::In this paper we report on the organization and expression of the two chalcone flavanone isomerase (CHI) genes A and B from thePetunia hybrida inbred line V30. From a combination of sequence data, primer extension and RNAse protection experiments we infer the presence of two promoters PA1 and PA2 upstream of the CHI g...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00036968
更新日期:1989-05-01 00:00:00
abstract::The caffeine biosynthetic pathway in coffee plants has been proposed to involve three distinct N -methyltransferases, xanthosine methyltransferase (XMT), 7- N -methylxanthine methyltransferase (MXMT; theobromine synthase), and 3,7-dimethylxanthine methyltransferase (DXMT; caffeine synthase). We previously isolated all...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-0393-x
更新日期:2004-04-01 00:00:00
abstract::To understand the transcriptional responses to flooding stress in roots including hypocotyl of soybean seedlings, genome-wide changes in gene expression were analyzed using a soybean microarray chip containing 42,034 60-mer oligonucleotide probes. More than 6,000 of flooding-responsive genes in the roots including hyp...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9799-4
更新日期:2011-09-01 00:00:00
abstract::Mammalian E2F transcription factors are composed of E2F and DP subunits, and with their negative regulators, Rb-related proteins, govern expression of cell-division-related genes. Six E2Fs and two DPs are present in mammals, but only single E2F genes are known from wheat, tobacco and carrot. Here we show that E2Fs are...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1011848528377
更新日期:2001-11-01 00:00:00
abstract::The full-length nucleotide sequence of a barley (Hordeum vulgare L.) leaf mRNA, found to increase rapidly in amount during infection attempts by the powdery mildew fungus (Erysiphe graminis DC. ex Mérat), is reported. The mRNA encodes a polypeptide of 809 amino acid residues which, by sequence comparison, was identifi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023606
更新日期:1993-03-01 00:00:00
abstract::The location on the wheat chloroplast DNA map and the nucleotide sequences of the genes coding for tRNA GCC (Gly) (trnG-GCC), tRNA GUC (Asp) (trnD-GUC) and tRNA GCA (Cys) (trnC-GCA) have been determined. These three genes are located in the large single copy region of the chloroplast genome, about half-way between one...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF02418249
更新日期:1985-09-01 00:00:00
abstract:KEY MESSAGE:The OsPLS2 locus was isolated and cloned by map-based cloning that encodes a Upf1-like helicase. Disruption of OsPLS2 accelerated light-dependent leaf senescence in the rice mutant of ospls2. Leaf senescence is a very complex physiological process controlled by both genetic and environmental factors, howeve...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00848-4
更新日期:2019-05-01 00:00:00
abstract:KEY MESSAGE:AcGST1, an anthocyanin-related GST, may functions as a carrier to transport anthocyanins from ER to tonoplast in kiwifruit. It was positively regulated by AcMYBF110 through directly binding to its promoter. Anthocyanins are synthesized in the cytoplasmic surface of the endoplasmic reticulum but accumulate p...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00870-6
更新日期:2019-07-01 00:00:00
abstract::Cyclins are involved in the regulation of cell cycle progression in eukaryotes. We have isolated a cyclin cDNA clone, cycZm2w, from maize root tip cells, which fits best into group A2 of current plant cyclin gene classification schemes. The cDNA encodes a protein with a domain homologous to the cyclin box of mitotic c...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005968901298
更新日期:1998-05-01 00:00:00
abstract::The promoter region (-309 to +44) of the Brassica napus storage protein gene napA was studied in transgenic tobacco by successive 5' as well as internal deletions fused to the reporter gene GUS (beta-glucuronidase). The expression in the two main tissues of the seed, the endosperm and the embryo, was shown to be diffe...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00041385
更新日期:1996-12-01 00:00:00
abstract::Elicitins are 10 kDa holoproteins secreted by Phytophthora fungi, that elicit an incompatible hypersensitive reaction, leading to resistance against fungal and bacterial plant pathogens. Comparison of primary sequences of alpha-elicitins and beta-elicitins indicated several potential necrotic activity-determining resi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019323
更新日期:1995-02-01 00:00:00
abstract::Neutral/Alkaline invertases are unique to plant and photosynthetic bacteria. The function of Neutral/Alkaline invertases in plant development is not clear so far. In this study, we isolated an Arabidopsis (Col-0) mutant insensitive to osmotic stress-induced inhibition on lateral root growth. Map-based cloning reveals ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9177-4
更新日期:2007-07-01 00:00:00
abstract::An approximately 247-kb genomic region from FF genome of wild rice Oryza brachyantha, possessing the smallest Oryza genome, was compared to the orthologous approximately 450-kb region from AA genome, O. sativa L. ssp. japonica. 37 of 38 genes in the orthologous regions are shared between japonica and O. brachyantha. A...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9178-3
更新日期:2007-07-01 00:00:00
abstract::We have characterized the topoisomerase I and II activities in nuclear extracts from immature embryos of Zea mays and the effect of the treatment with 2,4-dichlorophenoxyacetic acid (2,4-D) and abscisic acid (ABA). These extracts were shown to be essentially devoid of protease and nuclease activities and they were tes...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017917
更新日期:1991-01-01 00:00:00
abstract:KEY MESSAGE:This study provides the first genetic evidence for the role of PP2A in tuberization, demonstrating that the catalytic subunit StPP2Ac2b positively modulates tuber induction, and that its function is related to the regulation of gibberellic acid metabolism. The results contribute to a better understanding of...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0555-7
更新日期:2017-02-01 00:00:00
abstract::The aminoglycoside antibiotic hygromycin B (Hyg) inhibits prokaryotic, chloroplast and mitochondrial protein synthesis. Because of the toxic effect of Hyg on plant cells, the HPT gene, encoding hygromycin phosphotransferase, has become one of the most widely used selectable markers in plant transformation. Yet the mec...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-015-0380-4
更新日期:2015-12-01 00:00:00
abstract::Clones encoding beta-1,3-glucanase have been isolated from a Hevea cDNA library prepared from the latex of Hevea brasiliensis using a probe Nicotiana plumbaginifolia cDNA encoding beta-1,3-glucanase, gnl. Nucleotide sequence analysis showed that a 1.2 kb Hevea cDNA encoding a basic beta-1,3-glucanase showed 68% nucleo...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00043663
更新日期:1995-10-01 00:00:00
abstract::The photoreceptor UV RESISTANCE LOCUS 8 (UVR8) specifically mediates photomorphogenic responses to UV-B wavelengths. UVR8 acts by regulating transcription of a set of genes, but the underlying mechanisms are unknown. Previous research indicated that UVR8 can associate with chromatin, but the specificity and functional...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0522-3
更新日期:2016-11-01 00:00:00
abstract::The chloroplast gene for the epsilon subunit (atpE) of the CF1/CF0 ATPase in the green alga Chlamydomonas reinhardtii has been localized and sequenced. In contrast to higher plants, the atpE gene does not lie at the 3' end of the beta subunit (atpB) gene in the chloroplast genome of C. reinhardtii, but is located at a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00025326
更新日期:1987-03-01 00:00:00
abstract:KEY MESSAGE:RNA-seq analysis on whitebark pine needles demonstrated that methyl jasmonate (MeJA)-triggered transcriptome re-programming substantially overlapped with defense responses against insects and fungal pathogens in Pinus species, increasing current knowledge regarding induced systemic resistance (ISR) to patho...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-017-0655-z
更新日期:2017-11-01 00:00:00
abstract:KEY MESSAGE:Based on the nature of the proteins that are altered in abundance, we conclude that guard cells switch their energy source from fatty acid metabolism to chloroplast activity, at the onset of dawn. During stomatal opening at dawn, evidence was recently presented for a breakdown and liquidation of stored tria...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-018-0794-x
更新日期:2018-12-01 00:00:00
abstract::The identification of a cDNA (MR19) corresponding to a maize alpha-tubulin and homologous genomic clones (MG19/6 and MG19/14) is described. The cDNA has been isolated by differential screening of a cDNA maize root library. We have found two alpha-tubulin genes in a tandem arrangement in the genomic clones, separated b...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015650
更新日期:1990-01-01 00:00:00
abstract::Young pine seedlings respond to environmental stress by induced synthesis of pinosylvin, a stilbene phytoalexin. Heartwood of pine trees is characterized by a high content of pinosylvin. The formation of pinosylvin from cinnamoyl-CoA and three molecules malonyl-CoA catalysed by pinosylvin synthase is typical of the ge...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006163030646
更新日期:1999-01-01 00:00:00
abstract::Plant S-adenosyl-L-methionine-dependent methyltransferases (SAM-Mtases) are the key enzymes in phenylpropanoid, flavonoid and many other metabolic pathways of biotechnological importance. Here we compiled the amino acid sequences of 56 SAM-Mtases from different plants and performed a computer analysis for the conserve...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006035210889
更新日期:1998-07-01 00:00:00
abstract::Silicon (Si) confers several benefits to many plant species when absorbed as silicic acid through nodulin 26-like intrinsic proteins (NIPs). The NIPs belong to major intrinsic protein (MIP) family, members of which form channels with high selectivity to control transport of water and different solutes. Here, comparati...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0087-3
更新日期:2013-11-01 00:00:00
abstract::The monoterpene indole alkaloids (MIAs) from Madagascar periwinkle (Catharanthus roseus) are secondary metabolites of high interest due to their therapeutical values. Secologanin, the monoterpenoid moiety incorporated into MIAs, is derived from the plastidial methyl-D: -erythritol 4-phosphate (MEP) pathway. Here, we h...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9190-7
更新日期:2007-09-01 00:00:00
abstract::Nodules of tropical legumes generally export symbiotically fixed nitrogen in the form of ureides that are produced by oxidation of de novo synthesized purines. To investigate the regulation of de novo purine biosynthesis in these nodules, we have isolated cDNA clones encoding 5-aminoimidazole ribonucleotide (AIR) carb...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020176
更新日期:1994-01-01 00:00:00
abstract::CaaX-prenyltransferases (CaaX-PTases) catalyse the covalent attachment of isoprenyl groups to conserved cysteine residues located at the C-terminal CaaX motif of a protein substrate. This post-translational modification is required for the function and/or subcellular localization of some transcription factors and comp...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-3095-0
更新日期:2005-04-01 00:00:00
abstract::The complete nucleotide sequence of the rDNA large spacer region was determined for two related legume plants, Vicia faba and Pisum sativum. In the large spacers of both plants, there were one or several tandem arrays of repeated sequences which account well for the length heterogeneity of the rDNA repeating units. Wh...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019395
更新日期:1990-06-01 00:00:00
abstract::The expression of Brazil nut storage albumin genes is highly regulated during seed development. Several sequences in the promoter of one of these genes show homologies with the target sites of the maize O2 bZIP regulatory protein. We therefore asked whether the O2 protein would recognize these promoter sequences. We s...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005874404706
更新日期:1997-08-01 00:00:00