Application of RNAi to confirm theobromine as the major intermediate for caffeine biosynthesis in coffee plants with potential for construction of decaffeinated varieties.

abstract：KEY MESSAGE:Progression of the infection canal that conducts rhizobia to the nodule primordium requires a functional Rab GTPase located in Golgi/trans-Golgi that also participate in root hair polar growth. Common bean (Phaseolus vulgaris) symbiotically associates with its partner Rhizobium etli, resulting in the format...

journal_title：Plant molecular biology

authors： Dalla Via V,Traubenik S,Rivero C,Aguilar OM,Zanetti ME,Blanco FA

更新日期：2017-04-01 00:00:00

abstract：:Complex signal transduction pathways underlie the myriad plant responses to attack by pathogens. Ca(2+) is a universal second messenger in eukaryotes that modulates various signal transduction pathways through stimulus-specific changes in its intracellular concentration. Ca(2+)-binding proteins such as calmodulin (CaM...

journal_title：Plant molecular biology

authors： Chiasson D,Ekengren SK,Martin GB,Dobney SL,Snedden WA

更新日期：2005-08-01 00:00:00

abstract：:To gain insight into the regulatory mechanisms and the signals responsible for the adaptation of higher plants to conditions of varying sulfate availability, we have isolated from a sulfate-deprived root library maize cDNAs encoding sulfate permease (ZmST1) and ATP sulfurylase (ZmAS1), the two earliest components of t...

journal_title：Plant molecular biology

authors： Bolchi A,Petrucco S,Tenca PL,Foroni C,Ottonello S

更新日期：1999-02-01 00:00:00

abstract：:Chimeric genes composed of the beta-phaseolin promoter, an alpha-zein coding sequence and its modified versions containing lysine codons, and a beta-zein polyadenylation signal were inserted into the genome of tobacco by Agrobacterium-mediated transformation. alpha-Zein mRNA levels in the transgenic tobacco seeds 20 d...

journal_title：Plant molecular biology

authors： Ohtani T,Galili G,Wallace JC,Thompson GA,Larkins BA

更新日期：1991-01-01 00:00:00

abstract：:A genomic library was prepared from Populus kitakamiensis and screened with the cDNA for an anionic peroxidase from P. kitakamiensis. One genomic clone was isolated that contained two tandemly oriented genes for anionic peroxidases, prxA3a and prxA4a. Both genes consisted of four exons and three introns; the introns h...

journal_title：Plant molecular biology

authors： Osakabe K,Koyama H,Kawai S,Katayama Y,Morohoshi N

更新日期：1995-07-01 00:00:00

abstract：:The characterization of a non-photosynthetic isoform of NADP-malic enzyme (NADP-ME) from maize roots, which represents nearly 7% of the total soluble protein of this tissue, was performed. The molecular properties of the purified protein, as well as the kinetic parameters determined, indicate that the NADP-ME isoform ...

journal_title：Plant molecular biology

authors： Maurino VG,Saigo M,Andreo CS,Drincovich MF

更新日期：2001-03-01 00:00:00

abstract：:The PsbS gene product (PSII-S) which is an integral subunit of photosystem II has recently been reported to be a new type of pigment-binding protein [11]. The chlorophylls of the PSII-S protein exhibit weak excitonic coupling and this protein is stable also in the absence of pigments. Here we investigated the expressi...

journal_title：Plant molecular biology

authors： Adamska I,Funk C,Renger G,Andersson B

更新日期：1996-07-01 00:00:00

abstract：:Angiosperm genomes show tremendous variability in genome size and chromosome number. Nevertheless, comparative genetic mapping has revealed genome collinearity of closely related species. Sequence-based comparisons were used to assess the conservation of gene arrangements. Numerous small rearrangements, insertions/del...

journal_title：Plant molecular biology

authors： Schmidt R

更新日期：2002-01-01 00:00:00

abstract：:The relative amounts of some chloroplast polypeptides were followed during greening of leaves from three different plant families. Oat, bean and spinach were the representatives of the Gramineae, Leguminosae and Chenopodiaceae, respectively. By using specific antibodies against subunits of the chloroplast protein comp...

journal_title：Plant molecular biology

authors： Nechushtai R,Nelson N

更新日期：1985-11-01 00:00:00

abstract：:In mammalian cells the product of the human retinoblastoma tumour suppressor gene (pRb) can recruit Rpd3-like histone deacetylases to repress transcription. In this study, we investigated whether this mechanism might also be relevant in plants and found both conserved and distinct features. The expression profiles of ...

journal_title：Plant molecular biology

authors： Rossi V,Locatelli S,Lanzanova C,Boniotti MB,Varotto S,Pipal A,Goralik-Schramel M,Lusser A,Gatz C,Gutierrez C,Motto M

更新日期：2003-02-01 00:00:00

abstract：:Screening of a lambda gt11 cDNA expression library of Euglena gracilis with antibodies directed against histones H2 from maize resulted in the isolation of a full-length cDNA for a histone H2A. The open-reading frame of 408 bp corresponded to a protein of 136 amino acid residues (14 kDa). Despite the presence of a pol...

journal_title：Plant molecular biology

authors： Saint-Guily A,Schantz ML,Schantz R

更新日期：1994-03-01 00:00:00

abstract：:Plant responses to abiotic stresses are coordinated by arrays of growth and developmental programs. Phytohormones such as abscisic acid (ABA) and indole-3-acetic acid (IAA) play critical roles in developmental progresses and environmental responses through complex signalling networks. However, crosstalk between the tw...

journal_title：Plant molecular biology

authors： Du H,Wu N,Chang Y,Li X,Xiao J,Xiong L

更新日期：2013-11-01 00:00:00

abstract：:Limonium bicolor, a typical recretohalophyte that lives in saline environments, excretes excessive salt to the environment through epidermal salt glands to avoid salt stress. The aim of this study was to screen for L. bicolor genes involved in salt secretion by high-throughput RNA sequencing. We established the experi...

journal_title：Plant molecular biology

authors： Yuan F,Lyu MJ,Leng BY,Zhu XG,Wang BS

更新日期：2016-06-01 00:00:00

abstract：:A chimeric gene consisting of the 5' flanking sequences of a rice glutelin gene (Gt3) linked to the chloramphenicol acetyltransferase (CAT) coding segment was introduced into tobacco via Agrobacterium tumefaciens-mediated transformation. CAT enzyme activity could be detected in extracts from seeds as early as 8 days a...

journal_title：Plant molecular biology

authors： Leisy DJ,Hnilo J,Zhao Y,Okita TW

更新日期：1990-01-01 00:00:00

abstract：:The giant knotweed Fallopia sachalinensis (Polygonaceae) synthesizes phenylacetonitrile (PAN) from L-phenylalanine when infested by the Japanese beetle Popillia japonica or treated with methyl jasmonate (MeJA). Here we identified (E/Z)-phenylacetaldoxime (PAOx) as the biosynthetic precursor of PAN and identified a cyt...

journal_title：Plant molecular biology

authors： Yamaguchi T,Noge K,Asano Y

更新日期：2016-06-01 00:00:00

abstract：:As part of a strategy to define differences in genome organization and expression between cytoplasmic male-sterile (CMS) and male-fertile (MF) sugar-beet mitochondria, cDNA libraries from both mitochondrial genotypes were constructed. Preliminary screening with ribosomal RNA gene probes identified candidate cDNA clone...

journal_title：Plant molecular biology

authors： Xue Y,Collin S,Davies DR,Thomas CM

更新日期：1994-04-01 00:00:00

abstract：:We used a functional screening method to isolate genes whose products elicit the hypersensitive response (HR) pathway of defense against plant pathogens. A cDNA library derived from tobacco leaves undergoing the HR was cloned into a tobacco mosaic virus (TMV)-based expression vector. Infectious transcripts were genera...

journal_title：Plant molecular biology

authors： Karrer EE,Beachy RN,Holt CA

更新日期：1998-03-01 00:00:00

abstract：:The maize (Zea mays L.) caffeic acid O-methyl-transferase (COMT) is a key enzyme in the biosynthesis of lignin. In this work we have characterized the involvement of COMT in the lignification process through the study of the molecular mechanisms involved in its regulation. The examination of the maize COMT gene promot...

journal_title：Plant molecular biology

authors： Fornalé S,Sonbol FM,Maes T,Capellades M,Puigdomènech P,Rigau J,Caparrós-Ruiz D

更新日期：2006-12-01 00:00:00

abstract：:Iron uptake and translocation in plants are important processes for both plant and human nutrition, whereas relatively little is known about the molecular mechanisms of iron transport within the plant body. Several reports have shown that yellow stripe 1 (YS1) and YS1-like (YSL) transporters mediate metal-phytosiderop...

journal_title：Plant molecular biology

authors： Aoyama T,Kobayashi T,Takahashi M,Nagasaka S,Usuda K,Kakei Y,Ishimaru Y,Nakanishi H,Mori S,Nishizawa NK

更新日期：2009-08-01 00:00:00

abstract：:The maize Cat2 gene was isolated by direct cloning and PCR. The clones were mapped and sequenced. The start site of transcription was determined by primer extension. Computer analysis of the 1.6 kb Cat2 promoter sequence has revealed an obvious TATA box, tow GC boxes, a putative GA response element, and several ACGT c...

journal_title：Plant molecular biology

authors： Guan L,Polidoros AN,Scandalios JG

更新日期：1996-03-01 00:00:00

abstract：:The functional homologues of vertebrate natriuretic peptides (NPs), the plant natriuretic peptides (PNPs), are a novel class of peptidic hormones that signal via guanosine 3',5'-cyclic monophosphate (cGMP) and systemically affect plant salt and water balance and responses to biotrophic plant pathogens. Although there ...

journal_title：Plant molecular biology

authors： Turek I,Gehring C

更新日期：2016-06-01 00:00:00

abstract：:Several signal transduction pathways play important roles in the sexual life cycle of Chlamydomonas. Nitrogen deprivation, perhaps sensed as a drop in intracellular [NH4+], triggers a signal transduction pathway that results in altered gene expression and the induction of the gametogenic pathway. Blue light triggers a...

journal_title：Plant molecular biology

authors： Quarmby LM

更新日期：1994-12-01 00:00:00

abstract：:Cab-1 is a complex genetic locus in tomato consisting of four clustered genes encoding chlorophyll a/b-binding polypeptide. Southern blot analysis of total tomato DNA with genomic clones corresponding to the Cab-1 locus has revealed the presence of a repetitive element in the 3 kb spacer regions between two of these g...

journal_title：Plant molecular biology

authors： Bernatzky R,Pichersky E,Malik VS,Tanksley SD

更新日期：1988-09-01 00:00:00

abstract：:We have analysed the expression of the 8-10 members of the gene family encoding the flavonoid biosynthetic enzyme chalcone synthase (CHS) from Petunia hybrida. During normal plant development only two members of the gene family (CHS-A and CHS-J) are expressed. Their expression is restricted to floral tissues mainly. A...

journal_title：Plant molecular biology

authors： Koes RE,Spelt CE,Mol JN

更新日期：1989-02-01 00:00:00

abstract：KEY MESSAGE:KEG is involved in mediating the proteasome-dependent degradation of FDH, a stress-responsive enzyme. The UPS may function to suppress FDH mediated stress responses under favorable growth conditions. Formate dehydrogenase (FDH) has been studied in bacteria and yeasts for the purpose of industrial applicatio...

journal_title：Plant molecular biology

authors： McNeilly D,Schofield A,Stone SL

更新日期：2018-02-01 00:00:00

abstract：:An antisense approach was used to attempt to determine the function of the highly abundant, tapetum-specific A9 transcript in microsporogenesis. A Brassica napus A9 cDNA clone was linked in sense and antisense orientations to the Arabidopsis thaliana A9 promoter and the resulting chimaeric genes introduced into B. nap...

journal_title：Plant molecular biology

authors： Turgut K,Barsby T,Craze M,Freeman J,Hodge R,Paul W,Scott R

更新日期：1994-01-01 00:00:00

abstract：:TGA1a is a well-characterized transcription factor that may mediate the root-specific and auxin-responsive expression of some plant genes. In tobacco, Southern blot and genomic cloning analyses have shown that TGA1a consists of at least four closely related genes. Since TGA1a belongs to the bZIP class of transcription...

journal_title：Plant molecular biology

authors： Miao ZH,Liu X,Lam E

更新日期：1994-04-01 00:00:00

abstract：:The DNA sequence composition of 526 dicot and 345 monocot intron sequences have been characterized using computational methods. Splice site information content and bulk intron and exon dinucleotide composition were determined. Positions 4 and 5 of 5' splice sites contain different statistically significant levels of i...

journal_title：Plant molecular biology

authors： White O,Soderlund C,Shanmugan P,Fields C

更新日期：1992-09-01 00:00:00

abstract：:Dehiscence of oilseed rape pods, commonly known as pod shatter, is a process of agronomic importance that results in seed loss causing yield reductions and carry-over of the crop into the following growing season. In an effort to understand the mechanisms underlying this developmental event, the changes in gene expres...

journal_title：Plant molecular biology

authors： Coupe SA,Taylor JE,Isaac PG,Roberts JA

更新日期：1994-01-01 00:00:00

abstract：:Arabidopsis thaliana encodes four Dicer-like (DCL) proteins and five dsRNA-binding (DRB) proteins. We have previously demonstrated that DCL4 specifically interacts with DRB4 in vitro. Here we describe the interaction between DCL4 and DRB4 in vivo. The phenotype of a mutant with a defect in DCL4 (dcl4-2) was similar to...

journal_title：Plant molecular biology

authors： Nakazawa Y,Hiraguri A,Moriyama H,Fukuhara T

更新日期：2007-04-01 00:00:00