Abstract：
:The functional homologues of vertebrate natriuretic peptides (NPs), the plant natriuretic peptides (PNPs), are a novel class of peptidic hormones that signal via guanosine 3',5'-cyclic monophosphate (cGMP) and systemically affect plant salt and water balance and responses to biotrophic plant pathogens. Although there is increasing understanding of the complex roles of PNPs in plant responses at the systems level, little is known about the underlying signaling mechanisms. Here we report isolation and identification of a novel Leucine-Rich Repeat (LRR) protein that directly interacts with A. thaliana PNP, AtPNP-A. In vitro binding studies revealed that the Arabidopsis AtPNP-A binds specifically to the LRR protein, termed AtPNP-R1, and the active region of AtPNP-A is sufficient for the interaction to occur. Importantly, the cytosolic part of the AtPNP-R1, much like in some vertebrate NP receptors, harbors a catalytic center diagnostic for guanylyl cyclases and the recombinant AtPNP-R1 is capable of catalyzing the conversion of guanosine triphosphate to cGMP. In addition, we show that AtPNP-A causes rapid increases of cGMP levels in wild type (WT) leaf tissue while this response is significantly reduced in the atpnp-r1 mutants. AtPNP-A also causes cGMP-dependent net water uptake into WT protoplasts, and hence volume increases, whereas responses of the protoplasts from the receptor mutant are impaired. Taken together, our results suggest that the identified LRR protein is an AtPNP-A receptor essential for the PNP-dependent regulation of ion and water homeostasis in plants and that PNP- and vertebrate NP-receptors and their signaling mechanisms share surprising similarities.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Turek I,Gehring Cdoi
10.1007/s11103-016-0465-8subject
Has Abstractpub_date
2016-06-01 00:00:00pages
275-86issue
3eissn
0167-4412issn
1573-5028pii
10.1007/s11103-016-0465-8journal_volume
91pub_type
杂志文章abstract：:Abscisic acid (ABA) is one of the central plant hormones, responsible for controlling both maturation and germination in seeds, as well as mediating adaptive responses to desiccation, injury, and pathogen infection in vegetative tissues. Thorough analyses of two barley genes, HVA1 and HVA22, indicate that their respon...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-006-9017-y
更新日期：2006-09-01 00:00:00
abstract：:Ribose-5-phosphate isomerase (RPI) catalyses the interconversion of ribose-5-phosphate and ribulose-5-phosphate in the reductive and oxidative pentose phosphate pathways in plants. RPI from spinach chloroplasts was purified and microsequenced. Via PCR with degenerate primers designed against microsequenced peptides, a...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019012
更新日期：1996-02-01 00:00:00
abstract：:The relative amounts of some chloroplast polypeptides were followed during greening of leaves from three different plant families. Oat, bean and spinach were the representatives of the Gramineae, Leguminosae and Chenopodiaceae, respectively. By using specific antibodies against subunits of the chloroplast protein comp...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF02418259
更新日期：1985-11-01 00:00:00
abstract：:T-DNA tagging is a high throughput strategy for identifying and cloning functional genes in plants. In this study, we screened 4416 lab-created T(1) rice T-DNA tagged lines and identified a mutant, designated dh1 (degenerated hull1), with phenotype of degenerated hull and naked pistils and stamens. Approximately 60% f...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-007-9283-3
更新日期：2008-03-01 00:00:00
abstract：:Aquaporin belongs to a highly conserved group of membrane proteins called major intrinsic proteins that facilitate water transport across biological membranes. The genome of Arabidopsis encodes 35 aquaporin genes with 13 homologs in the plasma membrane intrinsic protein (PIP) subgroup. However, the function of each in...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/B:PLAN.0000040900.61345.a6
更新日期：2004-03-01 00:00:00
abstract：:In higher eudicotyledonous angiosperms the floral organs are typically arranged in four different whorls, containing sepals, petals, stamens and carpels. According to the ABC model, the identity of these organs is specified by floral homeotic genes of class A, A+B, B+C and C, respectively. In contrast to the sepal and...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1025070827979
更新日期：2003-07-01 00:00:00
abstract：:R2R3-MYB genes play a pivotal role in regulating anthocyanin accumulation. Here, we report two tandemly duplicated R2R3-MYB genes in peach, PpMYB10.1 and PpMYB10.2, with the latter showing lower ability to induce anthocyanin accumulation than the former. Site-directed mutation assay revealed two amino acid changes in ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-018-0773-2
更新日期：2018-09-01 00:00:00
abstract：:In previous work, a 33 kDa cysteine proteinase was found in callus initiated from maize (Zea mays L.) resistant to fall armyworm feeding. A callus cDNA library from the maize inbred Mp708 was screened with oligonucleotides derived from the N-terminal amino acid sequence of the 33 kDa proteinase and several cDNA clones...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1026494813936
更新日期：1999-05-01 00:00:00
abstract：:The Arabidopsis ga1 mutant has very low levels of endogenous, active gibberellins and thus has an extreme dwarf phenotype; application of GA3 induces stem elongation and flower development. To test the hypothesis that GA action in this system involves changes in gene expression, we have cloned mRNAs whose abundance ch...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00023557
更新日期：1994-02-01 00:00:00
abstract：:Maize and tomato cDNA clones have been hybridized in Southern blotting experiments to plant genomic DNA prepared from different lines to detect restriction fragment polymorphisms (RFPs). In maize we have found that a high degree of genetic variability is present, even among domestic inbred lines. Most randomly chosen ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020093
更新日期：1985-03-01 00:00:00
abstract：:Alternatively spliced introns are the ones that are usually spliced but can be occasionally retained in a transcript isoform. They are the most frequently used alternative splice form in plants (~50% of alternative splicing events). Chlamydomonas reinhardtii, a unicellular alga, is a good model to understand alternati...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-017-0605-9
更新日期：2017-06-01 00:00:00
abstract：:The expression of a number of plant genes is regulated by an endogenous circadian clock. We report that the Arabidopsis NIA2 (nitrate reductase) gene shows robust circadian oscillations in mRNA accumulation which persist for at least 5 days in plants that have been grown in a light-dark (LD) cycle and then transformed...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00029010
更新日期：1993-10-01 00:00:00
abstract：:The photoreceptor UV RESISTANCE LOCUS 8 (UVR8) specifically mediates photomorphogenic responses to UV-B wavelengths. UVR8 acts by regulating transcription of a set of genes, but the underlying mechanisms are unknown. Previous research indicated that UVR8 can associate with chromatin, but the specificity and functional...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0522-3
更新日期：2016-11-01 00:00:00
abstract：KEY MESSAGE:Our study firstly elaborated the underlying mechanism of endogenous CH4-induced abiotic tolerance, along with an alteration of ABA sensitivity by mimicking the endogenous CH4 production in MtMCR transgenic Arabidopsis. Endogenous methane (CH4) production and/or emission have been ubiquitously observed in st...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-019-00914-x
更新日期：2019-11-01 00:00:00
abstract：:A cDNA encoding the multifunctional cytochrome P450, CYP71E1, involved in the biosynthesis of the cyanogenic glucoside dhurrin from Sorghum bicolor (L.) Moench was isolated. A PCR approach based on three consensus sequences of A-type cytochromes P450- (V/I)KEX(L/F)R, FXPERF, and PFGXGRRXCXG-was applied. Three novel cy...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005915507497
更新日期：1998-02-01 00:00:00
abstract：:In yeast, Rad21/Scc1 and its meiotic variant Rec8 are key players in the establishment and subsequent dissolution of sister chromatid cohesion for mitosis and meiosis, respectively, which are essential for chromosome segregation. Unlike yeast, our identification revealed that the rice genome has 4 RAD21-like genes tha...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-005-4922-z
更新日期：2006-03-01 00:00:00
abstract：:By differential screening of a cDNA library of two-week-old rice seedlings cDNA clones were obtained, corresponding to shoot-specific mRNAs. By sequence analysis two of these clones were found to be rbcS cDNA clones. The mRNA corresponding to a third cDNA clone (COS5) displayed an expression pattern similar to the exp...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019157
更新日期：1990-09-01 00:00:00
abstract：:A drought- and abscisic acid (ABA)-inducible gene, His1, was isolated from Lycopersicon pennellii, a drought-resistant relative of cultivated tomato, and the gene structure was defined experimentally. The nucleotide sequence of His1 predicts a protein of 202 amino acid residues, with a significant sequence homology to...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020112
更新日期：1996-01-01 00:00:00
abstract：:The plant senescence syndrome resembles, in many molecular and phenotypic aspects, plant responses to abiotic stresses. Both processes have an enormous negative global agro-economic impact and endanger food security worldwide. Premature plant senescence is the main cause of losses in grain filling and biomass yield du...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1007/s11103-013-0038-z
更新日期：2013-08-01 00:00:00
abstract：:Left- and right-border repeats, which surround the T-region, contain two conserved regions separated by 5 bp that are not conserved. At the onset of T-DNA processing virD-encoded proteins introduce a nick in the largest of these conserved regions (12 bp) at a specific position in the bottom strand between a guanine an...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00027312
更新日期：1989-11-01 00:00:00
abstract：KEY MESSAGE:1599 novel circRNAs and 1583 heat stress-specific circRNAs were identified in Arabidopsis. Heat stress enhanced accumulation of circRNAs remarkably. Heat stress altered the sizes of circRNAs, numbers of circularized exons and alterative circularization events. A putative circRNA-mediated ceRNA networks unde...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-017-0684-7
更新日期：2018-02-01 00:00:00
abstract：:The 12-oxo-phytodienoic acid reductases (OPRs) are enzymes that catalyze the reduction of double bonds adjacent to an oxo group in alpha,beta-unsaturated aldehydes or ketones. Some of them have very high substrate specificity and are part of the octadecanoid pathway which convert linolenic acid to the phytohormone jas...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-005-8883-z
更新日期：2005-09-01 00:00:00
abstract：:The complex capitulum of Chrysanthemum morifolium is often comprised of bilaterally symmetrical ray florets and radially symmetrical disc florets. The TCP transcription factor clade CYCLOIDEA2 (CYC2) appears to play a vital role in determining floral symmetry and in regulating floral organ development in Asteraceae. O...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-020-00981-5
更新日期：2020-05-01 00:00:00
abstract：:We previously identified a cluster of d ormancy-a ssociated M ADS-box transcription factors (DAM genes) in peach [Prunus persica (L.) Batsch] as potential candidates for control of the non-dormant phenotype observed in the evg mutant. Of these genes, DAM3, DAM5 and DAM6 were winter expressed, suggesting a role for the...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-010-9608-5
更新日期：2010-05-01 00:00:00
abstract：:The nopaline synthase (nos) promoter is expressed in a wide range of plant cell types and regulated by various developmental and environmental factors. The nos upstream control region essential for this regulation was studied by means of synthetic oligomers using transient and stable transformation systems. Insertion ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040578
更新日期：1994-01-01 00:00:00
abstract：:Pleiotropic effects are one of the main concerns regarding genetically modified organisms (GMOs). This includes unintended side effects of the transgene or its genome insertion site on the regulation of other endogenous genes, which could potentially cause the accumulation of different secondary metabolites that may h...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-007-9229-9
更新日期：2007-11-01 00:00:00
abstract：:A new cytoplasmic male sterile sunflower, CMS3 [44], was characterised in relation to the Petiolaris (PET1) cytoplasmic male-sterile sunflower, CMS89 [25]. Southern blot analysis showed that the mitochondrial genome of CMS3 contains unique rearrangements in at least five loci (atp6, atp9, atpA, nad1 + 5 and coxIII) co...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019495
更新日期：1994-12-01 00:00:00
abstract：:In an attempt to identify domains directly involved in the signal transduction of phytochrome B (phyB), we over-expressed the achromophoric C-terminal half of phyB under control of the CaMV-35S promoter in transgenic Arabidopsis. In three independent transgenic lines, we detected accumulation of the introduced protein...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040726
更新日期：1996-08-01 00:00:00
abstract：:Peanut (Arachis hypogaea L.), can elicit type I allergy becoming the most common cause of fatal food-induced anaphylactic reactions. Strict avoidance is the only effective means of dealing with this allergy. Ara h 2, a peanut seed storage protein, has been identified as the most potent peanut allergen and is recognize...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9428-z
更新日期：2009-02-01 00:00:00
abstract：:Polyphenol oxidases (PPOs) catalyze the oxidation of ortho-diphenols to the corresponding quinones (EC 1.10.3.1). In plants PPOs appear in gene families, and the corresponding isoenzymes are located to the thylakoid lumen of chloroplasts. Although plant PPOs are often discussed with regard to their role in defense rea...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-012-9943-9
更新日期：2012-09-01 00:00:00
