Abstract：
:Dehiscence of oilseed rape pods, commonly known as pod shatter, is a process of agronomic importance that results in seed loss causing yield reductions and carry-over of the crop into the following growing season. In an effort to understand the mechanisms underlying this developmental event, the changes in gene expression that accompany pod shatter have been examined with a view to understanding how the process is regulated. In order to achieve this, cDNA library was constructed using mRNA extracted from the dehiscence zone of developing pods. Differential screening with non-dehiscence zone cDNA led to the isolation of a pod-specific clone, SAC25, with a transcript size of 1100 nucleotide encoding a predicted polypeptide of 34 kDa. The level of SAC25 mRNA accumulation increased during pod development. The sequence shows no significant homology to others within the databases but has two identifiable amino acid motifs, one is an adenine nucleotide binding site for NAD/FAD dehydrogenases and the other is a conserved feature of the ribitol dehydrogenase family. The amino acid sequence has four putative glycosylation sites and contains four cysteine residues. Genomic Southern analysis indicates that SAC25 may be encoded by a single gene or a small gene family. The function of this mRNA is unknown but possible roles in dehiscence and pod development are discussed.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Coupe SA,Taylor JE,Isaac PG,Roberts JAdoi
10.1007/BF00040589subject
Has Abstractpub_date
1994-01-01 00:00:00pages
223-7issue
1eissn
0167-4412issn
1573-5028journal_volume
24pub_type
杂志文章abstract：:As derived from a cDNA clone, the structure of the b-32 protein of Zea mays, a putative regulatory factor of zein expression, has a central acidic region separated by two domains covered by secondary structure motifs. In this work, three b-32 genomic clones were selected from two genomic libraries obtained from the ma...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019399
更新日期：1990-06-01 00:00:00
abstract：:The role of the cell wall hydrolase polygalacturonase (PG) during fruit ripening was investigated using novel mutant tomato lines in which expression of the PG gene has been down regulated by antisense RNA. Tomato plants were transformed with chimaeric genes designed to express anti-PG RNA constitutively. Thirteen tra...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00028773
更新日期：1990-03-01 00:00:00
abstract：:The microtubular element of the plant cytoskeleton undergoes dramatic architectural changes in the course of the cell cycle, specifically at the entry into and exit from mitosis. These changes underlie the acquisition of specialized properties and functions involved, for example, in the equal segregation of chromosome...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1023/a:1006346107807
更新日期：2000-08-01 00:00:00
abstract：:Rice storage protein glutelin genes are coordinately regulated during seed development. A previous 5' deletion analysis using transgenic tobacco revealed that the minimum 5' region necessary for endosperm specificity was within -245 bp of the transcription start site, and included the AACA and GCN4 motifs that are hig...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1026459229671
更新日期：1999-05-01 00:00:00
abstract：:A homologue of the multidrug resistance (MDR) gene was obtained while screening a potato stolon tip cDNA expression library with 35S-labeled calmodulin. The mammalian MDR gene codes for a membrane-bound P-glycoprotein (170-180 kDa) which imparts multidrug resistance to cancerous cells. The potato cDNA (PMDR1) codes fo...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00042240
更新日期：1996-06-01 00:00:00
abstract：:Catalase 2 (CAT2) plays an important role in the detoxification of hydrogen peroxide released either during photorespiration or as a consequence of biotic and abiotic stress as well as in the initiation of senescence. To date, our understanding of the regulation of CAT2 gene expression is rather poor. Chromatin immuno...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0546-8
更新日期：2017-01-01 00:00:00
abstract：KEY MESSAGE:The simultaneous expression of AmRosea1 and AmDelila transcription factors from snapdragon can activate the anthocyanin pathway in orange carrots, leading to the synthesis and accumulation of anthocyanins in the taproots. Anthocyanins are phenolic compounds produced in various parts of plants. They are used...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-020-01002-1
更新日期：2020-07-01 00:00:00
abstract：:A chimeric gene consisting of the 5' flanking sequences of a rice glutelin gene (Gt3) linked to the chloramphenicol acetyltransferase (CAT) coding segment was introduced into tobacco via Agrobacterium tumefaciens-mediated transformation. CAT enzyme activity could be detected in extracts from seeds as early as 8 days a...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00015653
更新日期：1990-01-01 00:00:00
abstract：:A gene for chlorophyll a/b-binding protein (cab) of pine (Pinus thumbergii) was isolated and sequenced. The gene (cab-6) contains an intron at a position equivalent to the type II cab genes of angiosperms. Transcript mapping analyses show that the amount of the mRNA in the dark is about half of that in the light. The ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00023388
更新日期：1992-06-01 00:00:00
abstract：:We describe a method for identifying signal transducing proteins from other organisms by their ability to turn on a signalling pathway when they are expressed at high level in yeast. The method was tested on a cDNA library from Arabidopsis thaliana, which was screened for clones that can activate glucose repression in...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1014440531842
更新日期：2002-05-01 00:00:00
abstract：:In previous work, a 33 kDa cysteine proteinase was found in callus initiated from maize (Zea mays L.) resistant to fall armyworm feeding. A callus cDNA library from the maize inbred Mp708 was screened with oligonucleotides derived from the N-terminal amino acid sequence of the 33 kDa proteinase and several cDNA clones...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1026494813936
更新日期：1999-05-01 00:00:00
abstract：:We describe the molecular and functional characterization of three closely related S-adenosyl-L-methionine synthetase (SAMS) isoenzymes from Catharanthus roseus (Madagascar periwinkle). The genes are differentially expressed in cell cultures during growth of the culture and after application of various stresses (elici...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005711720930
更新日期：1997-01-01 00:00:00
abstract：KEY MESSAGE:Combining with a CRISPR/Cas9 system, Agrobacterium-mediated transformation can lead to precise targeted T-DNA integration in the rice genome. Agrobacterium-mediated T-DNA integration into the plant genomes is random, which often causes variable transgene expression and insertional mutagenesis. Because T-DNA...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-018-00819-1
更新日期：2019-03-01 00:00:00
abstract：KEY MESSAGE:In response to an ultrasound pulse, several hundred DEGs, including in response to stress, were up- or down-regulated in in vitro potato plantlets. Despite this abiotic stress, plantlets survived. Ultrasound (US) can influence plant growth and development. To better understand the genetic mechanism underlyi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-019-00876-0
更新日期：2019-07-01 00:00:00
abstract：:This study describes the expression of a sucrose carrier at various developmental stages in Ricinus communis. A partial-length cDNA clone, RcSUT1, was isolated by RT-PCR from Ricinus seedling RNA. This is almost identical to a sucrose carrier cDNA, Rscr1, which has previously been isolated by library screening. Howeve...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006040306581
更新日期：1998-10-01 00:00:00
abstract：:Cassava mosaic disease (CMD) is a viral disease of the important tropical staple crop cassava (Manihot esculenta) and preferred management involves use of host-plant resistance. The best available resistance is controlled by a single dominant gene. Serial analysis of gene expression (SAGE) was used to analyze the gene...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-004-3477-8
更新日期：2004-11-01 00:00:00
abstract：:Opium poppy latex contains a group of laticifer-specific, low-molecular-weight polypeptides called major latex proteins (MLPs). Here we describe a new member of the MLP gene family (gMLP 22) which shares 79.6% nucleotide and 84.6% amino acid sequence identity with previously isolated clones. DNA gel blot analysis indi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00046460
更新日期：1992-11-01 00:00:00
abstract：:Transposable elements were first discovered in plants because they can have tremendous effects on genome structure and gene function. Although only a few or no elements may be active within a genome at any time in any individual, the genomic alterations they cause can have major outcomes for a species. All major eleme...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：
更新日期：2000-01-01 00:00:00
abstract：:CaaX-prenyltransferases (CaaX-PTases) catalyse the covalent attachment of isoprenyl groups to conserved cysteine residues located at the C-terminal CaaX motif of a protein substrate. This post-translational modification is required for the function and/or subcellular localization of some transcription factors and comp...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-005-3095-0
更新日期：2005-04-01 00:00:00
abstract：:To play an essential role in C4 photosynthesis, the maize C4 phosphoenolpyruvate carboxylase gene (PPCZm1) acquired many new expression features, such as leaf specificity, mesophyll specificity, light inducibility and high activity, that distinguish the unique C4 PPC from numerous non-C4 PPC genes in maize. We present...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006487326533
更新日期：2001-01-01 00:00:00
abstract：:APETALA2 (AP2) transcription factors (TFs) play very important roles in plant growth and development and in defense response. Here, we report functional characterization of four AP2 TF family genes [(RAP2.6 (At1g43160), RAP2.6L (At5g13330), DREB 26 (At1g21910) and DREB19 (At2g38340)] that were identified among NaCl in...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-010-9711-7
更新日期：2011-01-01 00:00:00
abstract：:Coordinated cell growth and differentiation is crucial for the development of higher plants. Using the elongation defective 1-1 (eld1-1) mutant, we cloned the ELD1 gene, which encodes a serine-rich protein. Genes homologous to ELD1 can be found in plants, including Arabidopsis, rice, and tobacco, but not in other orga...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/B:PLAN.0000019067.05185.d6
更新日期：2003-11-01 00:00:00
abstract：:A chimeric gene consisting of a bean (Phaseolus vulgaris L.) chalcone synthase (CHS) promoter fused to a bacterial chloramphenicol acetyltransferase (CAT) reporter gene was strongly expressed, and further induced by fungal elicitor, when electroporated into alfalfa (Medicago sativa L.) suspension cell protoplasts. Fun...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00015079
更新日期：1991-05-01 00:00:00
abstract：:Identification of downstream target genes of stress-relating transcription factors (TFs) is desirable in understanding cellular responses to various environmental stimuli. However, this has long been a difficult work for both experimental and computational practices. In this research, we presented a novel computationa...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9414-5
更新日期：2009-01-01 00:00:00
abstract：:Porphobilinogen deaminase (PBG deaminase) is an early enzyme of the pathway for chlorophyll and heme synthesis. Using degenerate oligonucleotide primers, based on amino acid sequence data for purified PBG deaminase from pea, a fragment was amplified from Arabidopsis genomic DNA by PCR, and then used to isolate both a ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00028854
更新日期：1994-11-01 00:00:00
abstract：:In higher eudicotyledonous angiosperms the floral organs are typically arranged in four different whorls, containing sepals, petals, stamens and carpels. According to the ABC model, the identity of these organs is specified by floral homeotic genes of class A, A+B, B+C and C, respectively. In contrast to the sepal and...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1025070827979
更新日期：2003-07-01 00:00:00
abstract：:The expression of the alpha' and beta subunit genes of beta-conglycinin is differentially regulated during soybean embryo development. Although both are expressed solely in developing seeds during mid to late stages of embryo development, the alpha' subunit is expressed more highly on a per gene basis, and alpha' subu...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00023991
更新日期：1991-03-01 00:00:00
abstract：:The folding and assembly of proteins in cells often requires the assistance of molecular chaperones such as the Hsp70 and Hsp60 heat shock proteins. Hsp70 chaperones cooperate with DnaJ and GrpE homologues to ensure a productive folding cycle. In this study we describe the gene for the first chloroplast localized DnaJ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005784115363
更新日期：1997-01-01 00:00:00
abstract：:Three polypeptides of 33 kD, 23 kD and 16 kD are released from maize photosystem II particles by alkaline Tris solution treatment and shown to cross-react with antisera to purified spinach oxygen-evolving (OE) polypeptides of 34 kD, 23 kD and 17 kD, respectively. They are not located exclusively in mesophyll cells but...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00166458
更新日期：1987-05-01 00:00:00
abstract：:Previous study showed that the magnesium-protoporphyrin IX chelatase H subunit (CHLH/ABAR) positively regulates abscisic acid (ABA) signaling. Here, we investigated the functions of a CHLH/ABAR interaction protein, the chloroplast co-chaperonin 20 (CPN20) in ABA signaling in Arabidopsis thaliana. We showed that down-e...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-013-0082-8
更新日期：2013-10-01 00:00:00
