Porphobilinogen deaminase is encoded by a single gene in Arabidopsis thaliana and is targeted to the chloroplasts.


:Porphobilinogen deaminase (PBG deaminase) is an early enzyme of the pathway for chlorophyll and heme synthesis. Using degenerate oligonucleotide primers, based on amino acid sequence data for purified PBG deaminase from pea, a fragment was amplified from Arabidopsis genomic DNA by PCR, and then used to isolate both a cDNA and a genomic clone for PBG deaminase from Arabidopsis. The cDNA, shown to be full-length by primer extension, encodes a precursor protein of 382 residues, which can be imported into isolated chloroplasts and processed to the mature size. The genomic clone encodes an identical sequence to the cDNA, except for the presence of four introns within the coding region of the mature protein, and 1.7 kb of upstream sequence. There is no obvious TATA box within 50 bp of the transcription start. Southern blot analysis suggests that PBG deaminase is encoded by a single gene in the Arabidopsis genome, and RNase protection experiments demonstrated that this gene is expressed in both leaves and roots. These results support the conclusion that there is only one form of PBG deaminase in all plant cells, which is located in the plastid.


Plant Mol Biol


Plant molecular biology


Lim SH,Witty M,Wallace-Cook AD,Ilag LI,Smith AG




Has Abstract


1994-11-01 00:00:00












  • Dawn regulates guard cell proteins in Arabidopsis thaliana that function in ATP production from fatty acid beta-oxidation.

    abstract:KEY MESSAGE:Based on the nature of the proteins that are altered in abundance, we conclude that guard cells switch their energy source from fatty acid metabolism to chloroplast activity, at the onset of dawn. During stomatal opening at dawn, evidence was recently presented for a breakdown and liquidation of stored tria...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Geilfus CM,Lan J,Carpentier S

    更新日期:2018-12-01 00:00:00

  • The presence of CYP79 homologues in glucosinolate-producing plants shows evolutionary conservation of the enzymes in the conversion of amino acid to aldoxime in the biosynthesis of cyanogenic glucosides and glucosinolates.

    abstract::A cDNA encoding CYP79B1 has been isolated from Sinapis alba. CYP79B1 from S. alba shows 54% sequence identity and 73% similarity to sorghum CYP79A1 and 95% sequence identity to the Arabidopsis T42902, assigned CYP79B2. The high identity and similarity to sorghum CYP79A1, which catalyses the conversion of tyrosine to p...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Bak S,Nielsen HL,Halkier BA

    更新日期:1998-11-01 00:00:00

  • Localization of AtROP4 and AtROP6 and interaction with the guanine nucleotide dissociation inhibitor AtRhoGDI1 from Arabidopsis.

    abstract::The small GTPases of the Rho family play a key role in actin cytoskeletal organization. In plants, a novel Rho subfamily, called ROP (Rho of plants), has been found. In Arabidopsis, 12 ROP GTPases have been identified which differ mainly at their C-termini. To test the localization of two members of this subfamily (At...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Bischoff F,Vahlkamp L,Molendijk A,Palme K

    更新日期:2000-02-01 00:00:00

  • OsbZIP71, a bZIP transcription factor, confers salinity and drought tolerance in rice.

    abstract::The bZIP transcription factor (TF) family plays an important role in the abscisic acid (ABA) signaling pathway of abiotic stress in plants. We here report the cloning and characterization of OsbZIP71, which encodes a rice bZIP TF. Functional analysis showed that OsbZIP71 is a nuclear-localized protein that specificall...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Liu C,Mao B,Ou S,Wang W,Liu L,Wu Y,Chu C,Wang X

    更新日期:2014-01-01 00:00:00

  • Structure and expression of a gene encoding the large subunit of ribulose-1,5-bisphosphate carboxylase (rbcL) in the colourless euglenoid flagellate Astasia longa.

    abstract::A gene encoding the large subunit of ribulose-1,5-bisphosphate carboxylase (Rubisco) was identified on a circular 73 kb DNA from the colourless euglenoid flagellate Astasia longa. The rbcL gene of Astasia extends over 3968 bp. It is a split gene interrupted by seven introns as compared to nine intervening sequences in...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Siemeister G,Hachtel W

    更新日期:1990-05-01 00:00:00

  • Characterization of a novel cis-acting element that is responsive to a fungal elicitor in the promoter of a tobacco class I chitinase gene.

    abstract::The expression of tobacco class I chitinase gene is effectively induced by a fungal elicitor in suspension-cultured tobacco cells. To identify cis-acting DNA elements that respond to the elicitor, a series of promoter constructs of the chitinase gene CHN50 fused to beta-glucuronidase gene was introduced into tobacco c...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Fukuda Y,Shinshi H

    更新日期:1994-02-01 00:00:00

  • The 22 bp W1 element in the pea lectin promoter is necessary and, as a multimer, sufficient for high gene expression in tobacco seeds.

    abstract::The pea lectin (Psl) gene encodes an abundant seed protein. Its seed-specific expression pattern is conserved in transgenic tobacco plants. Progressive 5' promoter deletions resulted in a gradual decrease of transcriptional activity in tobacco seed. A fragment of 115 bp still conferred seed-specific expression albeit ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: de Pater S,Pham K,Klitsie I,Kijne J

    更新日期:1996-11-01 00:00:00

  • Silencing of an α-dioxygenase gene, Ca-DOX, retards growth and suppresses basal disease resistance responses in Capsicum annum.

    abstract::Alpha-dioxygenases (α-DOX) catalyzing the primary oxygenation of fatty acids to oxylipins were recently found in plants. Here, the biological roles of the pepper α-DOX (Ca-DOX) gene, which is strongly induced during non-host pathogen infection in chili pepper, were examined. Virus-induced gene silencing demonstrated t...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Hong CE,Ha YI,Choi H,Moon JY,Lee J,Shin AY,Park CJ,Yoon GM,Kwon SY,Jo IH,Park JM

    更新日期:2017-03-01 00:00:00

  • Heterotopic expression of class B floral homeotic genes supports a modified ABC model for tulip (Tulipa gesneriana).

    abstract::In higher eudicotyledonous angiosperms the floral organs are typically arranged in four different whorls, containing sepals, petals, stamens and carpels. According to the ABC model, the identity of these organs is specified by floral homeotic genes of class A, A+B, B+C and C, respectively. In contrast to the sepal and...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Kanno A,Saeki H,Kameya T,Saedler H,Theissen G

    更新日期:2003-07-01 00:00:00

  • Identification and characterization of (GA/CT)n-microsatellite loci from Quercus petraea.

    abstract::In this study a size selected genomic library from Quercus petraea was screened for (GA/CT)n-microsatellite sequences. The resulting loci were analysed by PCR for their usefulness as molecular markers in Q. petraea and Q. robur. 17 out of 52 tested primer pairs resulted in the amplification of a polymorphic single-loc...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Steinkellner H,Fluch S,Turetschek E,Lexer C,Streiff R,Kremer A,Burg K,Glössl J

    更新日期:1997-04-01 00:00:00

  • Overexpression of the MYB29 transcription factor affects aliphatic glucosinolate synthesis in Brassica oleracea.

    abstract:KEY MESSAGE:Overexpression of BoMYB29 gene up-regulates the aliphatic glucosinolate pathway in Brassica oleracea plants increasing the production of the anti-cancer metabolite glucoraphanin, and the toxic and pungent sinigrin. Isothiocyanates, the bio-active hydrolysis products of glucosinolates, naturally produced by ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Zuluaga DL,Graham NS,Klinder A,van Ommen Kloeke AEE,Marcotrigiano AR,Wagstaff C,Verkerk R,Sonnante G,Aarts MGM

    更新日期:2019-09-01 00:00:00

  • Serial analysis of gene expression (SAGE) of host-plant resistance to the cassava mosaic disease (CMD).

    abstract::Cassava mosaic disease (CMD) is a viral disease of the important tropical staple crop cassava (Manihot esculenta) and preferred management involves use of host-plant resistance. The best available resistance is controlled by a single dominant gene. Serial analysis of gene expression (SAGE) was used to analyze the gene...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Fregene M,Matsumura H,Akano A,Dixon A,Terauchi R

    更新日期:2004-11-01 00:00:00

  • Promoter analysis of seed storage protein genes from Canavalia gladiata D.C.

    abstract::A number of A/T-rich sequences and a CATGCAT/A sequence are contained in the 5'-upstream regions of the genes encoding concanavalin A (Con A) and canavalin, two major seed storage proteins of Canavalia gladiata D.C. To study the role of these sequences in the seed-specific gene expression, we constructed 5'-deletion m...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Yamamoto S,Nishihara M,Morikawa H,Yamauchi D,Minamikawa T

    更新日期:1995-02-01 00:00:00

  • Characterization of the plasma membrane proteins and receptor-like kinases associated with secondary vascular differentiation in poplar.

    abstract::The constituents of plasma membrane proteins, particularly the integral membrane proteins, are closely associated with the differentiation of plant cells. Secondary vascular differentiation, which gives rise to the increase in plant stem diameter, is the key process by which the volume of the plant body grows. However...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Song D,Xi W,Shen J,Bi T,Li L

    更新日期:2011-05-01 00:00:00

  • Construction and characterisation of a yeast artificial chromosome library containing three haploid maize genome equivalents.

    abstract::We have constructed a yeast artificial chromosome (YAC) library using high-molecular-weight DNA prepared from agarose-embedded leaf protoplasts of the maize inbred line UE95. This library contains 79,000 clones with an average insert size of 145 kb and should therefore represent approximately three haploid genome equi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Edwards KJ,Thompson H,Edwards D,de Saizieu A,Sparks C,Thompson JA,Greenland AJ,Eyers M,Schuch W

    更新日期:1992-05-01 00:00:00

  • CYP71Z18 overexpression confers elevated blast resistance in transgenic rice.

    abstract:KEY MESSAGE:CYP71Z18 exhibited plastic substrate specificity to catalyze oxidation of multiple rice diterpenes and elevated chemical defense against the blast fungus in transgenic rice. Diversified plant specialized metabolism relies on corresponding biosynthetic enzymes with differential substrate specificity. CYP71Z1...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Shen Q,Pu Q,Liang J,Mao H,Liu J,Wang Q

    更新日期:2019-08-01 00:00:00

  • The effect of abscisic acid on the differential expression of α-amylase isozymes in barley aleurone layers.

    abstract::The treatment of barley aleurone layers with gibberellic acid (GA3) results in the synthesis of two groups of α-amylase isozymes. Addition of abscisic acid (ABA) at the same time as GA3 inhibited the synthesis of both groups of isozymes. However, midcourse ABA addition (12 h or later after GA3) had a more inhibitory e...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Nolan RC,Lin LS,Ho TH

    更新日期:1987-01-01 00:00:00

  • A tandem of alpha-tubulin genes preferentially expressed in radicular tissues from Zea mays.

    abstract::The identification of a cDNA (MR19) corresponding to a maize alpha-tubulin and homologous genomic clones (MG19/6 and MG19/14) is described. The cDNA has been isolated by differential screening of a cDNA maize root library. We have found two alpha-tubulin genes in a tandem arrangement in the genomic clones, separated b...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Montoliu L,Rigau J,Puigdomènech P

    更新日期:1990-01-01 00:00:00

  • The complex structures of arabinogalactan-proteins and the journey towards understanding function.

    abstract::Arabinogalactan-proteins (AGPs) are a family of complex proteoglycans found in all higher plants. Although the precise function(s) of any single AGP is unknown, they are implicated in diverse developmental roles such as differentiation, cell-cell recognition, embryogenesis and programmed cell death. DNA sequencing pro...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Gaspar Y,Johnson KL,McKenna JA,Bacic A,Schultz CJ

    更新日期:2001-09-01 00:00:00

  • Correction to: Differential expression of major genes involved in the biosynthesis of aliphatic glucosinolates in intergeneric Baemoochae (Brassicaceae) and its parents during development.

    abstract::Due to an unfortunate turn of events, an incorrect note was provided in the original publication as it should have read. ...

    journal_title:Plant molecular biology

    pub_type: 已发布勘误


    authors: Nugroho ABD,Han N,Pervitasari AN,Kim DH,Kim J

    更新日期:2020-03-01 00:00:00

  • Abnormal 'wrinkled' cell walls and retarded development of transgenic Arabidopsis thaliana plants expressing endo-1,4-beta-glucanase (cell) antisense.

    abstract::Transgenic Arabidopsis thaliana plants expressing cel1 antisense exhibit reduced levels of cel1 mRNA and protein compared with wild-type plants. The former display significant alterations in their phenotype. cel1 antisense plants have shorter stems and roots and are mechanically weaker than their wild-type counterpart...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Tsabary G,Shani Z,Roiz L,Levy I,Riov J,Shoseyov O

    更新日期:2003-01-01 00:00:00

  • Independent encapsidation of tomato golden mosaic virus A component DNA in transgenic plants.

    abstract::Tomato golden mosaic virus (TGMV), a member of the geminivirus group, has a genome consisting of two DNA molecules designated the A and B components. Both are required for infectivity in healthy plants, although the former has been shown to replicate independently in transgenic plants containing tandem direct repeats ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Sunter G,Gardiner WE,Rushing AE,Rogers SG,Bisaro DM

    更新日期:1987-11-01 00:00:00

  • Heterologous expression of cDNAs encoding Chlorella sorokiniana NADP-specific glutamate dehydrogenase wild-type and mutant subunits in Escherichia coli cells and comparison of kinetic and thermal stability properties of their homohexamers.

    abstract::Full-length cDNAs encoding the alpha- and beta-subunits and a truncated mutant subunit of the Chlorella sorokiniana NADP-GDH isozymes were constructed and expressed in Escherichia coli cells. The kinetic and thermal stability properties of the resultant homohexamers were examined. The electrophoretic mobility of the r...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Lawit SJ,Miller PW,Dunn WI,Mirabile JS,Schmidt RR

    更新日期:2003-06-01 00:00:00

  • FRIGIDA and related proteins have a conserved central domain and family specific N- and C- terminal regions that are functionally important.

    abstract::Arabidopsis accessions are either winter-annuals, which require cold winter temperatures for spring flowering, or rapid-cycling summer annuals. Typically, winter annual accessions have functional FRIGIDA (FRI) and FRIGIDA-LIKE 1 (FRL1) proteins that promote high expression of FLOWERING LOCUS C (FLC), which prevents fl...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Risk JM,Laurie RE,Macknight RC,Day CL

    更新日期:2010-07-01 00:00:00

  • RNA-Seq analysis and de novo transcriptome assembly of Hevea brasiliensis.

    abstract::Hevea brasiliensis, being the only source of commercial natural rubber, is an extremely economically important crop. In an effort to facilitate biological, biochemical and molecular research in rubber biosynthesis, here we report the use of next-generation massively parallel sequencing technologies and de novo transcr...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Xia Z,Xu H,Zhai J,Li D,Luo H,He C,Huang X

    更新日期:2011-10-01 00:00:00

  • Modification of reactive oxygen species scavenging capacity of chloroplasts through plastid transformation.

    abstract::Reactive oxygen species (ROS), including superoxide anions, hydrogen peroxide and hydroxyl radicals are generated through normal biochemical processes, but their production is increased by abiotic stresses. The prospects for enhancing ROS scavenging, and hence stress tolerance, by direct gene expression in a vulnerabl...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Poage M,Le Martret B,Jansen MA,Nugent GD,Dix PJ

    更新日期:2011-07-01 00:00:00

  • Organization and transcription of the class I phycoerythrin genes of the marine cyanobacterium Synechococcus sp. WH7803.

    abstract::The nucleotide sequences of the class I phycoerythrin (PE) alpha- and beta-subunit genes (cpeA and cpeB) from the marine cyanobacterium Synechococcus sp. WH7803 are reported. The cpeB gene is located upstream of cpeA with a separation of 56 nucleotides and the two genes are co-transcribed as a transcript of 1.3 kb, wi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Newman J,Mann NH,Carr NG

    更新日期:1994-02-01 00:00:00

  • Conservation of rice genetic resources: the role of the International Rice Genebank at IRRI.

    abstract::Rice genetic resources, comprising landrace varieties, modern and obsolete varieties, genetic stocks, breeding lines, and the wild rices, are the basis of world food security. The International Rice Genebank at the International Rice Research Institute in the Philippines conserves the largest and most diverse collecti...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审


    authors: Jackson MT

    更新日期:1997-09-01 00:00:00

  • The promoter of a Brassica napus lipid transfer protein gene is active in a range of tissues and stimulated by light and viral infection in transgenic Arabidopsis.

    abstract::cDNA and genomic clones encoding Brassica napus non-specific lipid transfer proteins (LTP) were isolated and sequenced. The encoded amino acid sequences were very similar to those reported previously for LTPs from B. napus and other species. Sequence information indicates that B. napus contains an LTP gene family. The...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Sohal AK,Pallas JA,Jenkins GI

    更新日期:1999-09-01 00:00:00

  • Characterization of three distinct cDNA clones encoding cysteine proteinases from maize (Zea mays L.) callus.

    abstract::In previous work, a 33 kDa cysteine proteinase was found in callus initiated from maize (Zea mays L.) resistant to fall armyworm feeding. A callus cDNA library from the maize inbred Mp708 was screened with oligonucleotides derived from the N-terminal amino acid sequence of the 33 kDa proteinase and several cDNA clones...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Pechan T,Jiang B,Steckler D,Ye L,Lin L,Luthe DS,Williams WP

    更新日期:1999-05-01 00:00:00