Abstract:
:We present evidence for a unique covalent modification of a nuclear-encoded precursor protein targeted to plant mitochondria. We investigated the early events of in vitro import for the mitochondrial precursor of the ATP synthase F1beta subunit from Nicotiana plumbaginifolia (pF1beta) into plant mitochondria. When pF1beta of 59 kDa was incubated with mitochondria isolated from different higher-plant species, a band of 61 kDa was generated. The 61 kDa protein was a covalently modified form of the 59 kDa pF1beta. The modification was dependent on the 25 amino acid long N-terminal region of the presequence of pF1beta. The modification was catalysed by an enzyme located in the outer mitochondrial membrane which was specific for higher plants and could not be washed off from the membrane by urea, KCl or EDTA. The modification was ATP- and Ca(2+)-dependent, but it was not affected by inhibitors of protein kinases. No inhibition of the modification was observed with phosphatase, methylation or acylation inhibitors. The modification occurs prior to translocation through the mitochondrial outer membrane. Inhibition of the modification process does not affect the import of the precursor protein, hence precursor modification was not a prerequisite for import. Both the modified and the unmodified pF1beta proteins were strongly associated with the mitochondrial outer membrane.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
von Stedingk E,Pavlov PF,Grinkevich VA,Glaser Edoi
10.1023/a:1006375123496subject
Has Abstractpub_date
1999-11-01 00:00:00pages
505-15issue
4eissn
0167-4412issn
1573-5028journal_volume
41pub_type
杂志文章abstract::A cDNA library was constructed from the root tissues of cassava variety Huanan 124 at the root bulking stage. A total of 9,600 cDNA clones from the library were sequenced with single-pass from the 5'-terminus to establish a catalogue of expressed sequence tags (ESTs). Assembly of the resulting EST sequences resulted i...
journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2010-12-01 00:00:00
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040617
更新日期:1992-11-01 00:00:00
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1997-04-01 00:00:00
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更新日期:1990-05-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2017-11-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1996-03-01 00:00:00
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pub_type: 杂志文章,已发布勘误
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更新日期:2018-02-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1994-11-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2019-05-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2006-03-01 00:00:00
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1996-02-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2010-04-01 00:00:00
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pub_type: 杂志文章
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