Abstract：
:Suppressive subtractive hybridisation was applied to the analysis of late stage arbuscular mycorrhizal development in pea. 96 cDNA clones were amplified and 81, which carried fragments more than 200 nt in size, were sequence analysed. Among 67 unique fragments, 10 showed no homology and 10 were similar to sequences with unknown function. RNA accumulation of the corresponding 67 genes was analysed by hybridisation of macro-arrays. The cDNAs used as probes were derived from roots of wild type and late mutant pea genotypes, inoculated or not with the AM fungus Glomus mosseae. After calibration, a more than 2.5-fold mycorrhiza-induced RNA accumulation was detected in two independent experiments in the wild type for 25 genes, 22 of which seemed to be induced specifically during late stage AM development. Differential expression for 7 genes was confirmed by RT-PCR using RNA from mycorrhiza and from controls of a different pea cultivar. In order to confirm arbuscule-related expression, the Medicago truncatula EST data base was screened for homologous sequences with putative mycorrhiza-related expression and among a number of sequences with significant similarities, a family of trypsin inhibitor genes could be identified. Mycorrhiza-induced RNA accumulation was verified for five members by real-time PCR and arbuscule-related activation of the promoter could be shown in transgenic roots for one of the genes, MtTi 1.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Grunwald U,Nyamsuren O,Tamasloukht M,Lapopin L,Becker A,Mann P,Gianinazzi-Pearson V,Krajinski F,Franken Pdoi
10.1007/s11103-004-1303-ysubject
Has Abstractpub_date
2004-07-01 00:00:00pages
553-66issue
4eissn
0167-4412issn
1573-5028pii
DO00001303journal_volume
55pub_type
杂志文章abstract：:Repetitive DNA sequences comprise a large percentage of plant genomes, and their characterization provides information about both species and genome evolution. We have isolated a recombinant clone containing a highly repeated DNA element (SB92) that is homologous to ca. 0.9% of the soybean genome or about 10(5) copies...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00041174
更新日期：1995-11-01 00:00:00
abstract：:The embryo essential gene EMB506 plays a crucial role in the transition of the Arabidopsis embryo from radial symmetry to bilateral symmetry just prior to the early heart stage of development. In addition to influencing embryo development EMB506 also affects chloroplast biogenesis. To further investigate the role of E...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-006-6268-6
更新日期：2006-05-01 00:00:00
abstract：:As a first step toward elucidating the in vivo function of plant bZIP proteins and their related G-box cis elements, we have introduced a dominant negative inhibitor of G-box-dependent transcriptional activation into tobacco plants by transforming them with a truncated EmBP-1 gene (deltaEmBP) containing the DNA bindin...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006081009173
更新日期：1998-11-01 00:00:00
abstract：:Miniature inverted-repeat transposable elements (MITEs) are short, non autonomous DNA elements that are widespread and abundant in plant genomes. The high sequence and size conservation observed in many MITE families suggest that they have spread recently throughout their respective host genomes. Here we present a mai...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-009-9511-0
更新日期：2009-09-01 00:00:00
abstract：:The purification of dihydrofolate reductase (5, 6, 7, 8 tetrahydrofolate: NADP(+) oxidoreductase, E.C.: 1.5.1.3) from Daucus carota to apparent homogeneity, is described. The enzyme is a soluble protein with a molecular weight of 183 000±2 500, composed of identical subunits of 58 400±1 000. The enzyme is only weakly ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00037557
更新日期：1985-11-01 00:00:00
abstract：:As part of a strategy to define differences in genome organization and expression between cytoplasmic male-sterile (CMS) and male-fertile (MF) sugar-beet mitochondria, cDNA libraries from both mitochondrial genotypes were constructed. Preliminary screening with ribosomal RNA gene probes identified candidate cDNA clone...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00024201
更新日期：1994-04-01 00:00:00
abstract：:Two hevein-like peptides from the seed of Pharbitis nil, designated Pharbitis nil antimicrobial peptide 1 (Pn-AMP1) and Pn-AMP2, had been purified previously. Both exhibit potent in vitro antifungal activity against a broad spectrum of phytopathogenic fungi. We now report the isolation of two cDNA clones, designated p...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1019864222515
更新日期：2002-10-01 00:00:00
abstract：:The first step in the routing of newly synthesized proteins into the secretory pathway is the binding of the nascent signal sequence to the signal recognition particle. The mammalian signal recognition particle is a complex consisting of 6 proteins and a single 7S RNA molecule. Signal recognition particle-like complex...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00042359
更新日期：1993-12-01 00:00:00
abstract：:The ubiquitous grapevine-associated octopine/cucumopine Ti plasmids of biotype III Agrobacterium tumefaciens strains carry two T regions, TA and TB, with a complex oncogene arrangement. Within the octopine/cucumopine group, two main strain types were identified: 'large TA' strains with a TA region resembling the TL re...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00018558
更新日期：1990-02-01 00:00:00
abstract：:We have constructed a chimaeric gene consisting of the promoter of the soybean heat shock (hs) gene Gmhsp17, 6-L, the coding region of a hygromycin phosphotransferase (hpt) gene, and the termination sequence of the nopaline synthase (nos) gene. This gene fusion was introduced into tobacco by Agrobacterium-mediated gen...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00039423
更新日期：1990-12-01 00:00:00
abstract：:Silicon (Si) confers several benefits to many plant species when absorbed as silicic acid through nodulin 26-like intrinsic proteins (NIPs). The NIPs belong to major intrinsic protein (MIP) family, members of which form channels with high selectivity to control transport of water and different solutes. Here, comparati...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-013-0087-3
更新日期：2013-11-01 00:00:00
abstract：:Due to an unfortunate turn of events, an incorrect note was provided in the original publication as it should have read. ...
journal_title：Plant molecular biology
pub_type： 已发布勘误
doi：10.1007/s11103-020-00970-8
更新日期：2020-03-01 00:00:00
abstract：:Vernalization, the requirement of a period of low temperature to induce transition from the vegetative to reproductive state, is an evolutionarily and economically important trait in the Triticeae. The genetic basis of vernalization in cultivated barley (Hordeum vulgare subsp. vulgare) can be defined using the two-loc...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-005-0351-2
更新日期：2005-10-01 00:00:00
abstract：KEY MESSAGE:To explore the function of Dof transcription factors during kernel development in maize, we first identified Dof genes in the maize genome. We found that ZmDof3 was exclusively expressed in the endosperm of maize kernel and had the features of a Dof transcription factor. Suppression of ZmDof3 resulted in a ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0543-y
更新日期：2017-01-01 00:00:00
abstract：:Salicylic acid (SA) is a stress-induced hormone involved in the activation of defense genes. Here we analyzed the early genetic responses to SA of wild type and npr1-1 mutant Arabidopsis seedlings, using Complete Arabidopsis Transcriptome MicroArray (CATMAv2) chip. We identified 217 genes rapidly induced by SA (early ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-009-9458-1
更新日期：2009-05-01 00:00:00
abstract：:Elicitins are 10 kDa holoproteins secreted by Phytophthora fungi, that elicit an incompatible hypersensitive reaction, leading to resistance against fungal and bacterial plant pathogens. Comparison of primary sequences of alpha-elicitins and beta-elicitins indicated several potential necrotic activity-determining resi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019323
更新日期：1995-02-01 00:00:00
abstract：:Arabinogalactan-proteins (AGPs) are a family of complex proteoglycans found in all higher plants. Although the precise function(s) of any single AGP is unknown, they are implicated in diverse developmental roles such as differentiation, cell-cell recognition, embryogenesis and programmed cell death. DNA sequencing pro...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：
更新日期：2001-09-01 00:00:00
abstract：:Cab-1 is a complex genetic locus in tomato consisting of four clustered genes encoding chlorophyll a/b-binding polypeptide. Southern blot analysis of total tomato DNA with genomic clones corresponding to the Cab-1 locus has revealed the presence of a repetitive element in the 3 kb spacer regions between two of these g...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00014948
更新日期：1988-09-01 00:00:00
abstract：:The bZIP transcription factor (TF) family plays an important role in the abscisic acid (ABA) signaling pathway of abiotic stress in plants. We here report the cloning and characterization of OsbZIP71, which encodes a rice bZIP TF. Functional analysis showed that OsbZIP71 is a nuclear-localized protein that specificall...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-013-0115-3
更新日期：2014-01-01 00:00:00
abstract：:Pathogens induce the expression of many genes encoding plant transcription factors, though specific knowledge of the biological function of individual transcription factors remains scarce. NAC transcription factors are encoded in plants by a gene family with proposed functions in both abiotic and biotic stress adaptat...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-007-9204-5
更新日期：2007-09-01 00:00:00
abstract：:14-3-3 proteins function as regulators of a wide range of target proteins in all eukaryotes by effecting direct protein-protein interactions. Primarily, interactions between 14-3-3 proteins and their targets are mediated by phosphorylation at specific sites on the target protein. Hence, interactions with 14-3-3s are s...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1023/a:1021261614491
更新日期：2002-12-01 00:00:00
abstract：:A new cytoplasmic male sterile sunflower, CMS3 [44], was characterised in relation to the Petiolaris (PET1) cytoplasmic male-sterile sunflower, CMS89 [25]. Southern blot analysis showed that the mitochondrial genome of CMS3 contains unique rearrangements in at least five loci (atp6, atp9, atpA, nad1 + 5 and coxIII) co...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019495
更新日期：1994-12-01 00:00:00
abstract：:Pleiotropic effects are one of the main concerns regarding genetically modified organisms (GMOs). This includes unintended side effects of the transgene or its genome insertion site on the regulation of other endogenous genes, which could potentially cause the accumulation of different secondary metabolites that may h...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-007-9229-9
更新日期：2007-11-01 00:00:00
abstract：KEY MESSAGE:RNA-seq analysis on whitebark pine needles demonstrated that methyl jasmonate (MeJA)-triggered transcriptome re-programming substantially overlapped with defense responses against insects and fungal pathogens in Pinus species, increasing current knowledge regarding induced systemic resistance (ISR) to patho...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-017-0655-z
更新日期：2017-11-01 00:00:00
abstract：:The sequence of the S-adenosyl-L-methionine:trans-caffeoyl-CoA O-methyltransferase (CCoAOMT, EC2.1.1.104) gene, including the 5'-flanking region of 5 kb, was determined from parsley (Petroselinum crispum) plants. The enzyme appears to be encoded by one or two genes, and the ORF is arranged in five exons spaced by intr...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005780529457
更新日期：1997-01-01 00:00:00
abstract：:The presence of specific glycine-rich proteins (GRP) related to petunia GRP1 (ptGRP1) was examined in three species of monocots (wheat, barley and maize) and five species of dicots (rape, turnip, soybean, crabapple and tomato). Protein blot analysis showed that anti-ptGRP1 antibody cross-reacted with a single differen...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020616
更新日期：1996-04-01 00:00:00
abstract：:Polyploidization has repeatedly occurred during plant evolution. Although autopolyploidy is the best model to characterize the polyploidization effects in a highly controlled manner, there are limited studies on autopolyploids compared to allopolyploids. To improve our understanding of autopolyploidy effects in maize,...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-010-9722-4
更新日期：2011-02-01 00:00:00
abstract：:Plant isoprenoids are derived from two independent pathways, the cytosolic mevalonate pathway and the plastid methylerythritol 4-phosphate (MEP) pathway. We used green fluorescent fusion protein assays to demonstrate that the Arabidopsis MEP pathway enzymes are localized to the chloroplast. We have also characterized ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9297-5
更新日期：2008-04-01 00:00:00
abstract：:To understand the transcriptional responses to flooding stress in roots including hypocotyl of soybean seedlings, genome-wide changes in gene expression were analyzed using a soybean microarray chip containing 42,034 60-mer oligonucleotide probes. More than 6,000 of flooding-responsive genes in the roots including hyp...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-011-9799-4
更新日期：2011-09-01 00:00:00
abstract：:Expression of the light-harvesting complex protein genes (Lhc) is under the control of a circadian clock. To dissect the molecular regulatory components of the circadian clock a promoter deletion analysis of four tomato Lhc genes was performed in transgenic tobacco plants. The important 5'-upstream promoter regions ar...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006094015513
更新日期：1998-11-01 00:00:00
