Molecular and structural characterization of barley vernalization genes.

abstract：:A chimeric gene consisting of the 5' flanking sequences of a rice glutelin gene (Gt3) linked to the chloramphenicol acetyltransferase (CAT) coding segment was introduced into tobacco via Agrobacterium tumefaciens-mediated transformation. CAT enzyme activity could be detected in extracts from seeds as early as 8 days a...

journal_title：Plant molecular biology

authors： Leisy DJ,Hnilo J,Zhao Y,Okita TW

更新日期：1990-01-01 00:00:00

abstract：:The complex capitulum of Chrysanthemum morifolium is often comprised of bilaterally symmetrical ray florets and radially symmetrical disc florets. The TCP transcription factor clade CYCLOIDEA2 (CYC2) appears to play a vital role in determining floral symmetry and in regulating floral organ development in Asteraceae. O...

journal_title：Plant molecular biology

authors： Yuan C,Huang D,Yang Y,Sun M,Cheng T,Wang J,Pan H,Zhang Q

更新日期：2020-05-01 00:00:00

abstract：:High molecular weight double-stranded (ds) RNAs have been detected in apparently virus-free French (common) bean Phaseolus vulgaris cv. Black Turtle Soup (BTS). Several other bean cultivars were free of detectable high molecular weight dsRNAs. The dsRNAs have been partially characterized and have homology to the BTS g...

journal_title：Plant molecular biology

authors： Wakarchuk DA,Hamilton RI

更新日期：1985-01-01 00:00:00

abstract：:The rice blast resistance gene Pib is a member of the nucleotide binding site (NBS) and leucine-rich repeat (LRR) class of plant disease resistance (R) genes and belongs to a small gene family. We describe here the isolation and characterization of a Pib homologue (PibH8), and extensive investigation of the expression...

journal_title：Plant molecular biology

authors： Wang ZX,Yamanouchi U,Katayose Y,Sasaki T,Yano M

更新日期：2001-11-01 00:00:00

abstract：:A cDNA library was made from poly(A)+ RNA isolated from developing Douglas fir (Pseudotsuga menziesii) embryo and megagametophytic tissue, and the cDNA clones were identified by immunoscreening with polyclonal antiserum against the crystalloid storage protein complex of Douglas fir. The nucleotide sequence of the long...

journal_title：Plant molecular biology

authors： Leal I,Misra S

更新日期：1993-02-01 00:00:00

abstract：:The synthesis, modification, and breakdown of carbohydrates is one of the most fundamentally important reactions in nature. The structural and functional diversity of glycosides is mirrored by a vast array of enzymes involved in their synthesis (glycosyltransferases), modification (carbohydrate esterases) and breakdow...

journal_title：Plant molecular biology

authors： Henrissat B,Coutinho PM,Davies GJ

更新日期：2001-09-01 00:00:00

abstract：KEY MESSAGE:To explore the function of Dof transcription factors during kernel development in maize, we first identified Dof genes in the maize genome. We found that ZmDof3 was exclusively expressed in the endosperm of maize kernel and had the features of a Dof transcription factor. Suppression of ZmDof3 resulted in a ...

journal_title：Plant molecular biology

authors： Qi X,Li S,Zhu Y,Zhao Q,Zhu D,Yu J

更新日期：2017-01-01 00:00:00

abstract：:In this paper we report on the organization and expression of the two chalcone flavanone isomerase (CHI) genes A and B from thePetunia hybrida inbred line V30. From a combination of sequence data, primer extension and RNAse protection experiments we infer the presence of two promoters PA1 and PA2 upstream of the CHI g...

journal_title：Plant molecular biology

authors： van Tunen AJ,Hartman SA,Mur LA,Mol JN

更新日期：1989-05-01 00:00:00

abstract：:Chimeric genes containing the coding sequence for bacterial chloramphenicol acetyl transferase (CAT) have been introduced by electroporation into maize protoplasts (Black Mexican Sweet) and transient expression monitored by enzyme assays. Levels of CAT expression were enhanced 12-fold and 20-fold respectively by the i...

journal_title：Plant molecular biology

authors： Mascarenhas D,Mettler IJ,Pierce DA,Lowe HW

更新日期：1990-12-01 00:00:00

abstract：KEY MESSAGE:Based on the nature of the proteins that are altered in abundance, we conclude that guard cells switch their energy source from fatty acid metabolism to chloroplast activity, at the onset of dawn. During stomatal opening at dawn, evidence was recently presented for a breakdown and liquidation of stored tria...

journal_title：Plant molecular biology

authors： Geilfus CM,Lan J,Carpentier S

更新日期：2018-12-01 00:00:00

abstract：:Two cDNAs encoding putative metallothionein (MT)-like peptides have been isolated from tomato (L. esculentum L.). The predicted protein products of these two cDNAs (LeMT(A) and LeMT(B)) are 72 and 83 amino acids respectively and both encode peptides with arrangements of cysteine residues characteristic of type II plan...

journal_title：Plant molecular biology

authors： Whitelaw CA,Le Huquet JA,Thurman DA,Tomsett AB

更新日期：1997-02-01 00:00:00

abstract：:Transposable elements were first discovered in plants because they can have tremendous effects on genome structure and gene function. Although only a few or no elements may be active within a genome at any time in any individual, the genomic alterations they cause can have major outcomes for a species. All major eleme...

journal_title：Plant molecular biology

authors： Bennetzen JL

更新日期：2000-01-01 00:00:00

abstract：:As an important osmoprotectant, glycine betaine (GB) plays an essential role in resistance to abiotic stress in a variety of organisms, including rice (Oryza sativa L.). However, GB content is too low to be detectable in rice, although rice genome possesses several orthologs coding for betaine aldehyde dehydrogenase (...

journal_title：Plant molecular biology

authors： Tang W,Sun J,Liu J,Liu F,Yan J,Gou X,Lu BR,Liu Y

更新日期：2014-11-01 00:00:00

abstract：:The identification of plant genes involved in early phases of in vitro morphogenesis can not only contribute to our understanding of the processes underlying growth regulator-controlled determination, but also provide novel markers for evaluating the outcome of in vitro regeneration experiments. To search for such gen...

journal_title：Plant molecular biology

authors： Torelli A,Soragni E,Bolchi A,Petrucco S,Ottonello S,Branca C

更新日期：1996-12-01 00:00:00

abstract：:With the completion of genomic sequencing of rice, rice has been firmly established as a model organism for both basic and applied research. The next challenge is to uncover the functions of genes predicted by sequence analysis. Considering the amount of effort and the diversity of disciplines required for functional ...

journal_title：Plant molecular biology

authors： Hirochika H,Guiderdoni E,An G,Hsing YI,Eun MY,Han CD,Upadhyaya N,Ramachandran S,Zhang Q,Pereira A,Sundaresan V,Leung H

更新日期：2004-02-01 00:00:00

abstract：:Cyclins are involved in the regulation of cell cycle progression in eukaryotes. We have isolated a cyclin cDNA clone, cycZm2w, from maize root tip cells, which fits best into group A2 of current plant cyclin gene classification schemes. The cDNA encodes a protein with a domain homologous to the cyclin box of mitotic c...

journal_title：Plant molecular biology

authors： Hsieh WL,Wolniak SM

更新日期：1998-05-01 00:00:00

abstract：:The ubiquitous grapevine-associated octopine/cucumopine Ti plasmids of biotype III Agrobacterium tumefaciens strains carry two T regions, TA and TB, with a complex oncogene arrangement. Within the octopine/cucumopine group, two main strain types were identified: 'large TA' strains with a TA region resembling the TL re...

journal_title：Plant molecular biology

authors： Huss B,Tinland B,Paulus F,Walter B,Otten L

更新日期：1990-02-01 00:00:00

abstract：KEY MESSAGE:This study provides the first genetic evidence for the role of PP2A in tuberization, demonstrating that the catalytic subunit StPP2Ac2b positively modulates tuber induction, and that its function is related to the regulation of gibberellic acid metabolism. The results contribute to a better understanding of...

journal_title：Plant molecular biology

authors： Muñiz García MN,Muro MC,Mazzocchi LC,País SM,Stritzler M,Schlesinger M,Capiati DA

更新日期：2017-02-01 00:00:00

abstract：:In the present study, we utilized TALEN- and CRISPR/Cas9-induced mutations to analyze the promoter of the barley phytase gene HvPAPhy_a. The purpose of the study was dual, validation of the PAPhy_a enzyme as the main contributor of the mature grain phytase activity (MGPA), as well as validating the importance of a spe...

journal_title：Plant molecular biology

authors： Holme IB,Wendt T,Gil-Humanes J,Deleuran LC,Starker CG,Voytas DF,Brinch-Pedersen H

更新日期：2017-09-01 00:00:00

abstract：:The characterization of the operon encoding the alpha and beta subunits of rhodophytan (R)-phycoerythrin (PE) from the macrophytic red alga Polysiphonia boldii is reported. This plastid-encoded operon was cloned, its nucleotide sequence determined, and its expression characterized by northern and primer extension anal...

journal_title：Plant molecular biology

authors： Roell MK,Morse DE

更新日期：1993-01-01 00:00:00

abstract：:Glyoxalase II is part of the glutathione-dependent glyoxalase detoxification system. In addition to its role in the detoxification of cytotoxic 2-oxo-aldehydes, specifically methylglyoxal, it has been suggested that the glyoxalase system may also play a role in controlling cell differentiation and proliferation. Durin...

journal_title：Plant molecular biology

authors： Maiti MK,Krishnasamy S,Owen HA,Makaroff CA

更新日期：1997-11-01 00:00:00

abstract：:A 7.4 kb segment of a Euglena nuclear gene encoding a portion of the polyprotein precursor to the light-harvesting chlorophyll a/b-binding protein of photosystem II (LHCPII) has been isolated and sequenced. Nine exons can be assembled into a continuous open reading frame encoding 113 amino acids of the C-terminus of a...

journal_title：Plant molecular biology

authors： Muchhal US,Schwartzbach SD

更新日期：1992-01-01 00:00:00

abstract：:The caffeine biosynthetic pathway in coffee plants has been proposed to involve three distinct N -methyltransferases, xanthosine methyltransferase (XMT), 7- N -methylxanthine methyltransferase (MXMT; theobromine synthase), and 3,7-dimethylxanthine methyltransferase (DXMT; caffeine synthase). We previously isolated all...

journal_title：Plant molecular biology

authors： Ogita S,Uefuji H,Morimoto M,Sano H

更新日期：2004-04-01 00:00:00

abstract：:Tomato fruit maturation is accompanied by a depolymerization of cell wall pectins which is due to the action of endopolygalacturonase (endoPG) preceded by pectin methylesterase (PE) activity. To investigate the role of endoPG and PE in determining the structure of green bean (Phaseolus vulgaris L.) pectins, these pect...

journal_title：Plant molecular biology

authors： Ebbelaar ME,Tucker GA,Laats MM,van Dijk C,Stolle-Smits T,Recourt K

更新日期：1996-09-01 00:00:00

abstract：:Two full-length cDNA clones (faEG1 and faEG3, respectively) have been isolated by screening a cDNA library representing transcripts from red strawberry fruits. Southern blot analysis of genomic DNA suggests that the strawberry endo-beta-1,4-glucanases (EGases) are encoded by a multigene family. The cognate genes are p...

journal_title：Plant molecular biology

authors： Trainotti L,Spolaore S,Pavanello A,Baldan B,Casadoro G

更新日期：1999-05-01 00:00:00

abstract：:Rice has developed several morphological and physiological strategies to adapt to phosphate starvation in the soil. In order to elucidate the molecular basis of response to phosphate starvation, we performed mRNA sequencing of 4 rice cultivars with variation in growth response to Pi starvation as indicated by the shoo...

journal_title：Plant molecular biology

authors： Oono Y,Kawahara Y,Yazawa T,Kanamori H,Kuramata M,Yamagata H,Hosokawa S,Minami H,Ishikawa S,Wu J,Antonio B,Handa H,Itoh T,Matsumoto T

更新日期：2013-12-01 00:00:00

abstract：:DNA methylation plays an important role in the gene-silencing network of higher eukaryotes. We have analyzed the 21.5-kb maintenance methyltransferase (M-MTase) gene, met1, of the multicellular green alga Volvox carteri. The met1 transcript was detected only during the period when DNA replication and cell division are...

journal_title：Plant molecular biology

authors： Babinger P,Völkl R,Cakstina I,Maftei A,Schmitt R

更新日期：2007-02-01 00:00:00

abstract：:The Arabidopsis ga1 mutant has very low levels of endogenous, active gibberellins and thus has an extreme dwarf phenotype; application of GA3 induces stem elongation and flower development. To test the hypothesis that GA action in this system involves changes in gene expression, we have cloned mRNAs whose abundance ch...

journal_title：Plant molecular biology

authors： Phillips AL,Huttly AK

更新日期：1994-02-01 00:00:00

abstract：:Developmentally regulated GTP-binding proteins (DRGs) from animals and fungi are highly conserved but have no known function. Here we characterize DRGs from pea (PsDRG) and Arabidopsis (AtDRG). Amino acid sequences of AtDRG and PsDRG were 90% identical to each other and about 65% identical to human DRG. Genomic Southe...

journal_title：Plant molecular biology

authors： Devitt ML,Maas KJ,Stafstrom JP

更新日期：1999-01-01 00:00:00

abstract：:Many chloroplast proteins are synthesized in the cytoplasm as precursors which contain an amino terminal transit peptide. These precursors are subsequently imported into chloroplast and targeted to one of several organellar locations. This import is mediated by the transit peptide, which is cleaved off during import. ...

journal_title：Plant molecular biology

authors： Smeekens S,van Steeg H,Bauerle C,Bettenbroek H,Keegstra K,Weisbeek P

更新日期：1987-07-01 00:00:00