Abstract：
:Developmentally regulated GTP-binding proteins (DRGs) from animals and fungi are highly conserved but have no known function. Here we characterize DRGs from pea (PsDRG) and Arabidopsis (AtDRG). Amino acid sequences of AtDRG and PsDRG were 90% identical to each other and about 65% identical to human DRG. Genomic Southern blotting indicated that AtDRG and PsDRG probably are single-copy genes. PsDRG mRNA accumulated preferentially in growing organs (root apices, growing axillary buds and elongating stems) compared with their non-growing counterparts. At DRG mRNA was relatively abundant in Arabidopsis leaves, stems and siliques, less abundant in flowers and flower buds, and barely detectable in roots. Histone mRNAs are known to accumulate predominantly during S phase of the cell cycle and are markers for proliferating cells. The patterns of histone H2A mRNA accumulation in pea and Arabidopsis organs were very similar to those of DRG mRNAs. An antiserum raised against a PsDRG N-terminal fusion protein recognized 43 and 45 kDa proteins. PsDRG proteins were more abundant in growing pea roots and stems than in non-growing organs, but they were equally abundant in growing and dormant axillary buds. After differential centrifugation, PsDRG proteins were found primarily in the microsomal (150,000 x g pellet) and soluble (150,000 x g supernatant) cell fractions.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Devitt ML,Maas KJ,Stafstrom JPdoi
10.1023/a:1006178710443subject
Has Abstractpub_date
1999-01-01 00:00:00pages
75-82issue
1eissn
0167-4412issn
1573-5028journal_volume
39pub_type
杂志文章abstract：:Bacteroids of Rhizobium leguminosarum in root nodules of Pisum sativum are enclosed by a plant-derived peribacteriod membrane (PBM). The contents of the interstitial peribacteroid space (PBS) between bacteroid membrane and PBM were isolated by a controlled osmotic shock of PBM-enclosed bacteroids and analysed by two-d...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00015670
更新日期：1988-03-01 00:00:00
abstract：:We have used the bar gene in combination with the herbicide Basta to select transformed rice (Oryza sativa L. cv. Radon) protoplasts for the production of herbicide-resistant rice plants. Protoplasts, obtained from regenerable suspension cultures established from immature embryo callus, were transformed using PEG-medi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00027118
更新日期：1993-03-01 00:00:00
abstract：:We have previously shown that the mitochondrial gene atpA, encoding the alpha subunit of F1 ATP synthase, is associated with DNA rearrangements and nuclear-specific transcript patterns in the male-sterile cytoplasm of Ogura radish. Here we present a detailed characterization of this gene from both the normal (fertile)...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00016123
更新日期：1990-11-01 00:00:00
abstract：:Hevea brasiliensis, being the only source of commercial natural rubber, is an extremely economically important crop. In an effort to facilitate biological, biochemical and molecular research in rubber biosynthesis, here we report the use of next-generation massively parallel sequencing technologies and de novo transcr...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-011-9811-z
更新日期：2011-10-01 00:00:00
abstract：:Transcription of plastid genes in higher plants is driven by two RNA polymerases. One is encoded in the chloroplast, the other is encoded in the nucleus. RNA synthesis in ribosome-deficient plastids is performed exclusively by the nuclear-encoded enzyme. In vitro capping was used to identify the transcriptional start ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005924502336
更新日期：1998-02-01 00:00:00
abstract：:We describe the isolation and characterization of maize cDNAs that are transcribed from a small gene family and encode a novel group of receptor-like kinases (RLKs). The distinctive extracellular domain of these novel RLKs includes a unique number and arrangement of leucine-rich repeats (LRRs), a proline-rich region (...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006012530241
更新日期：1998-07-01 00:00:00
abstract：:Screening of a lambda gt11 cDNA expression library of Euglena gracilis with antibodies directed against histones H2 from maize resulted in the isolation of a full-length cDNA for a histone H2A. The open-reading frame of 408 bp corresponded to a protein of 136 amino acid residues (14 kDa). Despite the presence of a pol...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00014447
更新日期：1994-03-01 00:00:00
abstract：:Ethylene promotes the abscission of leaves and the ripening of fruits in pepper plants, and in both events an increase in cellulase activity is observed. However, two enzyme isoforms (pI 7.2 and 8.5, respectively) are differentially involved in the two physiological phenomena. The pI 8.5 form has been purified from ri...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00041164
更新日期：1995-11-01 00:00:00
abstract：:In Brassica, the S-locus glycoprotein (SLG) gene has been strongly implicated in the self-incompatibility reaction. Several alleles of this locus have been sequenced, and accordingly grouped as class I (corresponding to dominant S-alleles) and class II (recessive). We recently showed that a self-compatible (Sc) line o...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00037027
更新日期：1995-03-01 00:00:00
abstract：:We isolated and characterized different classes of transposable DNA elements in oil palm (Elaeis guineensis) plants grown from seed, and plants regenerated from tissue culture that show mantling, an abnormality leading to flower abortion. Using PCR assays, reverse transcriptase fragments belonging to LINE-like and gyp...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1023942309092
更新日期：2003-05-01 00:00:00
abstract：:TGA1a is a well-characterized transcription factor that may mediate the root-specific and auxin-responsive expression of some plant genes. In tobacco, Southern blot and genomic cloning analyses have shown that TGA1a consists of at least four closely related genes. Since TGA1a belongs to the bZIP class of transcription...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00024193
更新日期：1994-04-01 00:00:00
abstract：KEY MESSAGE:Degradation of nitrogen-rich purines is tightly and oppositely regulated under drought and low nitrogen supply in bread wheat. Allantoin is a key target metabolite for improving nitrogen homeostasis under stress. The metabolite allantoin is an intermediate of the catabolism of purines (components of nucleot...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-019-00831-z
更新日期：2019-03-01 00:00:00
abstract：:cDNA clones for all enzymes of the prechorismate pathway of higher plants have previously been cloned, with the exception of the second enzyme of the pathway, 3-dehydroquinate synthase. Here we describe the isolation of a cDNA encoding a 3-dehydroquinate synthase from tomato which was identified by complementing a 3-d...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020607
更新日期：1996-04-01 00:00:00
abstract：:To isolate genes which are expressed preferentially during embryogenesis, a Douglas-fir embryogenesis cDNA library was constructed and differentially screened with cDNA probes made with mRNA from developing and mature embryos, respectively. The cDNA clone PM 2.1 was isolated based on its abundance in developing seeds ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005839832096
更新日期：1997-05-01 00:00:00
abstract：:The characterization of the operon encoding the alpha and beta subunits of rhodophytan (R)-phycoerythrin (PE) from the macrophytic red alga Polysiphonia boldii is reported. This plastid-encoded operon was cloned, its nucleotide sequence determined, and its expression characterized by northern and primer extension anal...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00039617
更新日期：1993-01-01 00:00:00
abstract：:DNA microsatellites are ubiquitously present in eukaryotic genomes and represent a vast source of highly informative markers. We describe in this article a (GGC)n microsatellite which is widely distributed in eukaryotic genomes. Using polymerase chain reaction (PCR) techniques and DNA sequencing, we demonstrated for t...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00014544
更新日期：1993-02-01 00:00:00
abstract：:We have isolated and analysed a 2 kb region of the mitochondrial genome of Arabidopsis thaliana (Columbia) showing a high level of nucleotide identity with the mitochondrial (mt) rps14 small-subunit ribosomal protein gene from Oenothera berteriana and Vicia faba, as well as with an open reading frame (ORF) located ups...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00028903
更新日期：1992-12-01 00:00:00
abstract：:DORNRÖSCHEN (DRN) and DORNRÖSCHEN-LIKE (DRNL) encode AP2-domain transcription factors, which act redundantly in cotyledon organogenesis. A more detailed genetic study now integrates DRN and DRNL into the CUP-SHAPED COTYLEDON (CUC) regulatory network and places DRN and DRNL differentially within the auxin signalling ne...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-010-9721-5
更新日期：2011-02-01 00:00:00
abstract：:A clone encoding a proline-rich protein (ZmPRP) has been obtained from maize root by differential screening of a maturing elongation root cDNA library. The amino acid sequence deduced from the full-length cDNA contains a putative signal peptide and a highly repetitive sequence containing the PEPK motif, indicating tha...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006129703262
更新日期：1999-03-01 00:00:00
abstract：:Iron (Fe) deficiency, a worldwide agricultural problem on calcareous soil with low Fe availability, is also a major human nutritional deficit. Plants induce Fe acquisition systems under conditions of low Fe availability. Previously, we reported that an Fe-deficiency-inducible basic helix-loop-helix (bHLH) transcriptio...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-011-9752-6
更新日期：2011-04-01 00:00:00
abstract：:In this report we characterized the Arabidopsis ABI1 gene orthologue and Brassica napus gene paralogues encoding protein phosphatase 2C (PP2C, group A), which is known to be a negative regulator of the ABA signaling pathway. Six homologous B. napus sequences were identified and characterized as putative PP2C group A m...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-015-0334-x
更新日期：2015-07-01 00:00:00
abstract：:Vernalization, the requirement of a period of low temperature to induce transition from the vegetative to reproductive state, is an evolutionarily and economically important trait in the Triticeae. The genetic basis of vernalization in cultivated barley (Hordeum vulgare subsp. vulgare) can be defined using the two-loc...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-005-0351-2
更新日期：2005-10-01 00:00:00
abstract：:Chloroplast and cytosolic triosephosphate isomerases from spinach were separated and purified to homogeneity. Both enzymes were partially sequenced by Edman degradation. Using degenerate primers designed against the amino acid sequences, a homologous probe for the chloroplast enzyme was amplified and used to isolate s...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019506
更新日期：1994-12-01 00:00:00
abstract：:Phaseolin is a glycoprotein that constitutes the major storage protein in bean seeds. The phaseolin gene promoters function in a seed-specific manner. In an attempt to understand if events following transcription of the gene also contribute to the seed-specific accumulation of the phaseolin protein, we studied the eff...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040527
更新日期：1992-09-01 00:00:00
abstract：:The major phytoalexin in alfalfa is the isoflavonoid (-)-medicarpin (or 6aR, 11aR)-medicarpin. Isoflavone reductase (IFR), the penultimate enzyme in medicarpin biosynthesis, is responsible for introducing one of two chiral centers in (-)-medicarpin. We have isolated a 1.18 kb alfalfa cDNA (pIFRalf1) which, when expres...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00037051
更新日期：1991-10-01 00:00:00
abstract：:Plants are capable to de novo synthesize the essential amino acids leucine, isoleucine and valine. Studies in recent years, however, also revealed that plants have the potential to degrade leucine or may be all of the branched-chain amino acids. One of the enzymes participating in both biosynthesis and degradation is ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-004-7533-1
更新日期：2005-01-01 00:00:00
abstract：:This study describes the expression of a sucrose carrier at various developmental stages in Ricinus communis. A partial-length cDNA clone, RcSUT1, was isolated by RT-PCR from Ricinus seedling RNA. This is almost identical to a sucrose carrier cDNA, Rscr1, which has previously been isolated by library screening. Howeve...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006040306581
更新日期：1998-10-01 00:00:00
abstract：KEY MESSAGE:TaPSK5 is a less conserved target of miR164 in wheat encoding a positive regulator of root growth and yield traits that could be used for crop improvement. MicroRNAs (miRNAs) play key roles in regulating plant growth and development by targeting the mRNAs of conserved genes. However, little is known about t...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-020-01064-1
更新日期：2020-12-01 00:00:00
abstract：:Higher-plant, green and red algal chloroplasts are surrounded by a double membrane envelope. The glaucocystophyte plastid (cyanelle) has retained a prokaryotic cell wall between the two envelope membranes. The complex chloroplasts of Euglena and dinoflagellates are surrounded by three membranes while the complex chlor...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：
更新日期：1998-09-01 00:00:00
abstract：KEY MESSAGE:This study provides the first genetic evidence for the role of PP2A in tuberization, demonstrating that the catalytic subunit StPP2Ac2b positively modulates tuber induction, and that its function is related to the regulation of gibberellic acid metabolism. The results contribute to a better understanding of...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0555-7
更新日期：2017-02-01 00:00:00
