Abstract:
:In Brassica, the S-locus glycoprotein (SLG) gene has been strongly implicated in the self-incompatibility reaction. Several alleles of this locus have been sequenced, and accordingly grouped as class I (corresponding to dominant S-alleles) and class II (recessive). We recently showed that a self-compatible (Sc) line of Brassica oleracea expressed a class II-like SLG (SLG-Sc) gene. Here, we report that the SLG-Sc glycoprotein is electrophoretically and immunochemically very similar to the recessive SLG-S15 glycoprotein, and is similarly expressed in stigmatic papillae. Moreover, by seed yield analysis, we observed that both alleles are associated with a self-compatibility response, in contrast with the other known recessive S haplotypes (S2 and S5). By genomic DNA blot analysis, we show the existence of molecular homologies between the Sc and S15 haplotypes, but demonstrate that they are not identical. On the other hand, we also report that the S2 haplotype expresses very low amounts of SLG glycoproteins, although it exhibits a self-incompatible phenotype. These results strongly question the precise role of the SLG gene in the molecular mechanisms that control the self-incompatibility reaction of Brassica.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Gaude T,Rougier M,Heizmann P,Ockendon DJ,Dumas Cdoi
10.1007/BF00037027subject
Has Abstractpub_date
1995-03-01 00:00:00pages
1003-14issue
5eissn
0167-4412issn
1573-5028journal_volume
27pub_type
杂志文章abstract::We have identified two single-copy genes from the model legume. Medicago truncatula (MtENOD16 and 20) whose expression can be correlated with early stages of root nodulation and whose predicted coding sequences are partially homologous to both pea/vetch ENOD5 and soybean N315/ENOD55. Database searching and sequence al...
journal_title:Plant molecular biology
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2003-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/BF00047719
更新日期:1992-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1985-07-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2011-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-03-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1990-06-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1994-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1994-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1990-08-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1995-03-01 00:00:00
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pub_type: 杂志文章
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