Abstract:
:Transcription of plastid genes in higher plants is driven by two RNA polymerases. One is encoded in the chloroplast, the other is encoded in the nucleus. RNA synthesis in ribosome-deficient plastids is performed exclusively by the nuclear-encoded enzyme. In vitro capping was used to identify the transcriptional start sites of the genes clpP and rpl23 in ribosome-free plastids of the barley mutant albostrians. No transcript initiation was found at sequences similar to eubacterial promoters. Instead, transcription started near the motif 5'-YRTA-3', which is also conserved in mitochondrial promoters of higher plants. Our data suggest that the nuclear encoded RNA polymerase is active in mature chloroplasts and is the sole polymerase involved in transcription of rpl123.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Hübschmann T,Börner Tdoi
10.1023/a:1005924502336subject
Has Abstractpub_date
1998-02-01 00:00:00pages
493-6issue
3eissn
0167-4412issn
1573-5028journal_volume
36pub_type
杂志文章abstract::Higher plant microspores, when subjected to various stress treatments in vitro, are able to reprogram their regular gametophytic development towards the sporophytic pathway to form haploid embryos and plants. Suppression subtractive hybridization (SSH) and metabolic profiling were used to characterize this development...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-9078-y
更新日期:2007-01-01 00:00:00
abstract::Exposure of seedlings of a chilling-sensitive variety of rice (Oryza sativa L. cv. Wasetoittu) to water stress (0.5 M mannitol, 30 min) at room temperature induced a degree of chilling resistance. No such resistance was induced by exogenous abscisic acid (ABA) application (10 microM, 60 min). Upon short-term water str...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00039544
更新日期:1994-10-01 00:00:00
abstract::Repair of the ubiquitous mutagenic lesion 7,8-dihydro-8-oxoguanine (8-oxoG) is initiated in eukaryotes by DNA glycosylases/lyases, such as yeast Ogg1, that do not share significant sequence identity with their prokaryotic counterparts, typified by Escherichia coli MutM (Fpg) protein. The unexpected presence of a funct...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1013644026132
更新日期:2001-12-01 00:00:00
abstract::Galactomannans are hemicellulosic polysaccharides composed of a (1 → 4)-linked β-D-mannan backbone substituted with single-unit (1 → 6)-α-linked D-galactosyl residues. Developing fenugreek (Trigonella foenum-graecum) seeds are known to accumulate large quantities of galactomannans in the endosperm, and were thus used ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-012-9909-y
更新日期:2012-06-01 00:00:00
abstract::Free Air [CO(2)] Enrichment (FACE) allows for plant growth under fully open-air conditions of elevated [CO(2)] at concentrations expected to be reached by mid-century. We used Arabidopsis thaliana ecotypes Col-0, Cvi-0, and WS to analyze changes in gene expression and metabolite profiles of plants grown in "SoyFACE" (...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-9041-y
更新日期:2006-11-01 00:00:00
abstract::We have cloned and sequenced a wound-inducible cDNA clone designated WIP1 (for wound-induced protein) from maize coleoptiles. It was isolated by differential screening of a cDNA library prepared from excised maize coleoptile segments. The deduced amino acid sequence predicts a secretory, cysteine-rich protein of 102 r...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027365
更新日期:1993-08-01 00:00:00
abstract::The effect of red light and far-red light on the appearance of mRNA for the small and large subunit of ribulose-1,5-bisphosphate carboxylase in rye seedlings has been analysed. Irradiation of etiolated seedlings with a pulse of red light increased the mRNA level of the small subunit by a factor of 7-10 and that of the...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00014183
更新日期:1987-01-01 00:00:00
abstract::Wood is the most abundant biological resource on earth and it is also an important raw material for a major global industry with rapidly increasing demand. The genus Eucalyptus includes the most widely used tree species for industrial plantation, mainly for making pulp and paper. With the aim of identifying major gene...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-0621-4
更新日期:2004-05-01 00:00:00
abstract::TGA1a is a well-characterized transcription factor that may mediate the root-specific and auxin-responsive expression of some plant genes. In tobacco, Southern blot and genomic cloning analyses have shown that TGA1a consists of at least four closely related genes. Since TGA1a belongs to the bZIP class of transcription...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00024193
更新日期:1994-04-01 00:00:00
abstract::A cDNA coding for phosphoenolpyruvate carboxylase (PEPC) was isolated from a cDNA library from Solanum tuberosum and the sequence of the cDNA was determined. It was inserted into a bacterial expression vector and a PEPC- Escherichia coli mutant could be complemented by the cDNA construct. A functional fusion protein c...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00021542
更新日期:1993-11-01 00:00:00
abstract::Leaf senescence is one of the key stages of plant leaf development. It is a highly complex but ordered process involving expression of large scale senescence associated genes, and its molecular mechanisms still remain unclear. By using suppression subtractive hybridization, 815 ESTs that are up-regulated at the onset ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9300-1
更新日期:2008-05-01 00:00:00
abstract::As a highly active plant viral promoter that is able to function in a wide variety of cell types, the cauliflower mosaic virus (CaMV) 35S promoter has the potential for harboring a plant enhancer element. We tested this possibility and demonstrated that a 338 base pair fragment isolated from the region upstream of the...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027403
更新日期:1988-05-01 00:00:00
abstract::In this paper we report on the organization and expression of the two chalcone flavanone isomerase (CHI) genes A and B from thePetunia hybrida inbred line V30. From a combination of sequence data, primer extension and RNAse protection experiments we infer the presence of two promoters PA1 and PA2 upstream of the CHI g...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00036968
更新日期:1989-05-01 00:00:00
abstract::Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor composed of NF-YA, NF-YB and NF-YC proteins. In this study, we identified and characterized a gene, GmNFYA3, which encodes the NF-YA subunit of the NF-Y complex in soybeans (Glycine max L.). Real time RT-PCR analysis indicated that GmNFYA3 was induced by...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0040-5
更新日期:2013-05-01 00:00:00
abstract::Homologous recombination is an essential process for the maintenance and variability of the genome. In eukaryotes, the Rad52 epistasis group proteins serve the main role for meiotic recombination and/or homologous recombinational repair. Rad51-like proteins, such as Rad55 and Rad57 in yeast, play a role in assembly or...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1016047231597
更新日期:2002-09-01 00:00:00
abstract::The chrysanthemum is one of popular flowers in the world and a host for several viruses. So far, molecular interaction studies between the chrysanthemum and viruses are limited. In this study, we carried out a transcriptome analysis of chrysanthemum in response to three different viruses including Cucumber mosaic viru...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-015-0317-y
更新日期:2015-06-01 00:00:00
abstract::Tree crops have a long juvenile period which is a serious constraint for genetic improvement and experimental research. For example, apple remains in a juvenile phase for more than five years after seed germination. Here, we report about induction of rapid flowering in apple seedlings using the Apple latent spherical ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9718-0
更新日期:2011-01-01 00:00:00
abstract::Plant infections with cassava latent virus (CLV) were mediated by the Ti plasmid of Agrobacterium tumefaciens containing either monomeric or dimeric copies of the virus genome. The CLV DNAs caused typical symptoms when they were inoculated in Agrobacterium strains C58, LBA4404 and a virE mutant A1026, but not other Ag...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019520
更新日期:1988-11-01 00:00:00
abstract::Recent studies with dicot plants reveal that floral organ development is controlled by a group of regulatory factors containing the MADS domain. In this study, we have isolated and characterized a cDNA clone from rice, OsMADS1, which encodes a MADS-domain-containing protein. The OsMADS1 amino acid sequence shows 56.2%...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00013751
更新日期:1994-10-01 00:00:00
abstract::A chimeric gene consisting of a bean (Phaseolus vulgaris L.) chalcone synthase (CHS) promoter fused to a bacterial chloramphenicol acetyltransferase (CAT) reporter gene was strongly expressed, and further induced by fungal elicitor, when electroporated into alfalfa (Medicago sativa L.) suspension cell protoplasts. Fun...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015079
更新日期:1991-05-01 00:00:00
abstract::Many eukaryotic genomes have experienced ancient whole-genome duplication (WGD) followed by massive gene loss. These eliminations were not random since some gene families were preferentially retained as duplicates. The gene balance hypothesis suggests that those genes with dosage reduction can imbalance their interact...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9639-y
更新日期:2010-07-01 00:00:00
abstract::Differential screening of a cDNA bank constructed from ripe tomato fruit mRNA allowed the isolation of cDNA clone 2A11 which is entirely fruit-specific, is expressed at steadily increasing levels from anthesis to breaker, and accounts for approximately 1% of the messenger RNA in mature tomato fruit. A genomic clone co...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00016019
更新日期:1989-12-01 00:00:00
abstract::Cytosine methylation provides an attractive epigenetic modification for the global maintenance of phases in plant development; however, there are few known examples of specific genes whose methylation status changes in a developmentally regulated manner. Pl-Blotched, an allele of purple plant1 (pl1), which encodes a m...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-5620-6
更新日期:2006-03-01 00:00:00
abstract::The rice blast resistance gene Pib is a member of the nucleotide binding site (NBS) and leucine-rich repeat (LRR) class of plant disease resistance (R) genes and belongs to a small gene family. We describe here the isolation and characterization of a Pib homologue (PibH8), and extensive investigation of the expression...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1012457113700
更新日期:2001-11-01 00:00:00
abstract::Arabidopsis thaliana encodes four Dicer-like (DCL) proteins and five dsRNA-binding (DRB) proteins. We have previously demonstrated that DCL4 specifically interacts with DRB4 in vitro. Here we describe the interaction between DCL4 and DRB4 in vivo. The phenotype of a mutant with a defect in DCL4 (dcl4-2) was similar to...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-9125-8
更新日期:2007-04-01 00:00:00
abstract::To elucidate the evolutionary relationship between the Spermatophyta, Pteridophyta and Bryophyta, we cloned a fragment of chloroplast DNA from the fern Angiopteris lygodiifolia (Pteridophyta) and determined its nucleotide sequence. The fragment contained the atpB, rbcL, trnR-CCG, dedB and psaI genes. Comparisons of th...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00018458
更新日期:1992-01-01 00:00:00
abstract:KEY MESSAGE:OsWRKY36 represses plant height and grain size by inhibiting gibberellin signaling. Plant height and grain size are important agronomic traits affecting yield in cereals, including rice. Gibberellins (GAs) are plant hormones that promote plant growth and developmental processions such as stem elongation and...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01049-0
更新日期:2020-11-01 00:00:00
abstract::Several populations of polyadenylated RNA from Vicia faba méristematic root cells were fractionated by stepwise thermal elution from poly(U)-Sepharose following sequential phenol extraction. Analysis of these fractions showed that the size of the poly(A) segment could influence this fractionation, but in some cases ot...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023013
更新日期:1981-03-01 00:00:00
abstract::The LfKCS45 gene with a high sequence similarity to known 3-ketoacyl-CoA synthases of the membrane-bound fatty acid elongase was isolated from Lesquerella fendleri. The LfKCS45 gene has a 1464 bp open reading frame without introns, and is predicted to encode a polypeptide of 487 amino acids with an estimated molecular...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-6235-z
更新日期:2004-12-01 00:00:00
abstract:KEY MESSAGE:IEF, a novel plasma plasma membrane protein, is important for exine formation in Arabidopsis. Exine, an important part of pollen wall, is crucial for male fertility. The major component of exine is sporopollenin which are synthesized and secreted by tapetum. Although sporopollenin synthesis has been well st...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01114-8
更新日期:2021-01-22 00:00:00