Characterisation of transcript initiation sites in ribosome-deficient barley plastids.

Abstract:

:Transcription of plastid genes in higher plants is driven by two RNA polymerases. One is encoded in the chloroplast, the other is encoded in the nucleus. RNA synthesis in ribosome-deficient plastids is performed exclusively by the nuclear-encoded enzyme. In vitro capping was used to identify the transcriptional start sites of the genes clpP and rpl23 in ribosome-free plastids of the barley mutant albostrians. No transcript initiation was found at sequences similar to eubacterial promoters. Instead, transcription started near the motif 5'-YRTA-3', which is also conserved in mitochondrial promoters of higher plants. Our data suggest that the nuclear encoded RNA polymerase is active in mature chloroplasts and is the sole polymerase involved in transcription of rpl123.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Hübschmann T,Börner T

doi

10.1023/a:1005924502336

subject

Has Abstract

pub_date

1998-02-01 00:00:00

pages

493-6

issue

3

eissn

0167-4412

issn

1573-5028

journal_volume

36

pub_type

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