GmNFYA3, a target gene of miR169, is a positive regulator of plant tolerance to drought stress.


:Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor composed of NF-YA, NF-YB and NF-YC proteins. In this study, we identified and characterized a gene, GmNFYA3, which encodes the NF-YA subunit of the NF-Y complex in soybeans (Glycine max L.). Real time RT-PCR analysis indicated that GmNFYA3 was induced by abscisic acid (ABA) and abiotic stresses, such as polyethylene glycol, NaCl and cold. Subcellular localization analysis suggested that GmNFYA3 may activate its specific targets in the nucleus. Histochemical β-glucuronidase (GUS) staining revealed that the expression of the GUS gene driven by the GmNFYA3 promoter occurred in various transgenic Arabidopsis tissues. Coexpression in Nicotiana benthamiana and 5' RACE assays indicated that miR169 directs GmNFYA3 mRNA cleavage in vivo. Overexpression of GmNFYA3 resulted in Arabidopsis with reduced leaf water loss and enhanced drought tolerance. In addition, the transgenic Arabidopsis exhibited increased sensitivity to high salinity and exogenous ABA. Moreover, the transcript levels of ABA biosynthesis (ABA1, ABA2), ABA signaling (ABI1, ABI2) and stress-responsive genes, including RD29A and CBF3, were generally higher in GmNFYA3 plants than in wild-type controls under normal conditions. These results suggest that the GmNFYA3 gene functions in positive modulation of drought stress tolerance and has potential applications in molecular breeding to enhance drought tolerance in crops.


Plant Mol Biol


Plant molecular biology


Ni Z,Hu Z,Jiang Q,Zhang H




Has Abstract


2013-05-01 00:00:00












  • Fractionation and characterization of polyadenylated RNA from broad bean meristematic root cells.

    abstract::Several populations of polyadenylated RNA from Vicia faba méristematic root cells were fractionated by stepwise thermal elution from poly(U)-Sepharose following sequential phenol extraction. Analysis of these fractions showed that the size of the poly(A) segment could influence this fractionation, but in some cases ot...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Trapy G,Favre A,Esnault R

    更新日期:1981-03-01 00:00:00

  • Genome-wide gene expression in an Arabidopsis cell suspension.

    abstract::Plant cell suspension cultures are invaluable models for the study of cellular processes. Here we develop the recently described Arabidopsis suspension culture MM2d as a transcript profiling platform by means of Affymetrix ATH1 microarrays. Analysis of gene expression profiles during normal culture growth, during sync...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Menges M,Hennig L,Gruissem W,Murray JA

    更新日期:2003-11-01 00:00:00

  • Two higher molecular weight forms related to ferritin are synthesizedin vitro by RNA from developing cotyledons and leaves ofPhaseolus vulgaris.

    abstract::Using RNA from developing cotyledons and leaves of bean two products were synthesizedin vitro, with Mr 31000 and 31500 which are antigenically related to the subunit of phytoferritin (Mr 26500). The relative abundance of the two products is dependent on the type of tissue from which the RNA was derived. Evidence is pr...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: van der Mark F,Klerk H,Bienfait F

    更新日期:1983-11-01 00:00:00

  • Cloning of a cDNA encoding a 3-dehydroquinate synthase from a higher plant, and analysis of the organ-specific and elicitor-induced expression of the corresponding gene.

    abstract::cDNA clones for all enzymes of the prechorismate pathway of higher plants have previously been cloned, with the exception of the second enzyme of the pathway, 3-dehydroquinate synthase. Here we describe the isolation of a cDNA encoding a 3-dehydroquinate synthase from tomato which was identified by complementing a 3-d...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


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    journal_title:Plant molecular biology

    pub_type: 杂志文章


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    更新日期:1997-05-01 00:00:00

  • A B-box zinc finger protein, MdBBX10, enhanced salt and drought stresses tolerance in Arabidopsis.

    abstract:KEY MESSAGE:The expression of MdBBX10 was significantly induced by different stresses and ABA treatments. Overexpression of MdBBX10 in Arabidopsis significantly enhanced abiotic stresses tolerance by ABA signalling. The roles of B-box domain(s) containing proteins (BBXs) in regulation of flowering and light morphogenes...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Liu X,Li R,Dai Y,Yuan L,Sun Q,Zhang S,Wang X

    更新日期:2019-03-01 00:00:00

  • The monomeric GTPase RabA2 is required for progression and maintenance of membrane integrity of infection threads during root nodule symbiosis.

    abstract:KEY MESSAGE:Progression of the infection canal that conducts rhizobia to the nodule primordium requires a functional Rab GTPase located in Golgi/trans-Golgi that also participate in root hair polar growth. Common bean (Phaseolus vulgaris) symbiotically associates with its partner Rhizobium etli, resulting in the format...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Dalla Via V,Traubenik S,Rivero C,Aguilar OM,Zanetti ME,Blanco FA

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    journal_title:Plant molecular biology

    pub_type: 杂志文章


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    journal_title:Plant molecular biology

    pub_type: 杂志文章


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  • Arabidopsis thaliana contains a large family of germin-like proteins: characterization of cDNA and genomic sequences encoding 12 unique family members.

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    journal_title:Plant molecular biology

    pub_type: 杂志文章


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    更新日期:1998-12-01 00:00:00

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    journal_title:Plant molecular biology

    pub_type: 杂志文章


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    journal_title:Plant molecular biology

    pub_type: 杂志文章


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    journal_title:Plant molecular biology

    pub_type: 杂志文章


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    更新日期:1994-04-01 00:00:00

  • The plant natriuretic peptide receptor is a guanylyl cyclase and enables cGMP-dependent signaling.

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    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Turek I,Gehring C

    更新日期:2016-06-01 00:00:00

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    journal_title:Plant molecular biology

    pub_type: 杂志文章


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    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Espartero J,Sánchez-Aguayo I,Pardo JM

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  • Evidence implying only unprimed RdRP activity during transitive gene silencing in plants.

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    journal_title:Plant molecular biology

    pub_type: 杂志文章


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  • IMPERFECTIVE EXINE FORMATION (IEF) is required for exine formation and male fertility in Arabidopsis.

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    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Wang K,Zhao X,Pang C,Zhou S,Qian X,Tang N,Yang N,Xu P,Xu X,Gao J

    更新日期:2021-01-22 00:00:00

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    journal_title:Plant molecular biology

    pub_type: 杂志文章


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  • The molecular basis of resistance to the herbicide norflurazon.

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    journal_title:Plant molecular biology

    pub_type: 杂志文章


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    更新日期:1991-06-01 00:00:00

  • The molecular and biochemical basis for varietal variation in sesquiterpene content in melon (Cucumis melo L.) rinds.

    abstract::A combined chemical, biochemical and molecular study was conducted to understand the differential accumulation of volatile sesquiterpenes in melon fruits. Sesquiterpenes were present mainly in the rinds of climacteric varieties, and a great diversity in their composition was found among varieties. Sesquiterpenes were ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Portnoy V,Benyamini Y,Bar E,Harel-Beja R,Gepstein S,Giovannoni JJ,Schaffer AA,Burger J,Tadmor Y,Lewinsohn E,Katzir N

    更新日期:2008-04-01 00:00:00

  • Arabidopsis CBF5 interacts with the H/ACA snoRNP assembly factor NAF1.

    abstract::The conserved protein CBF5, initially regarded as a centromere binding protein in yeast and higher plants, was later found within nucleoli and in Cajal bodies of yeast and metazoa. There, it is assumed to be involved in posttranscriptional pseudouridinylation of various RNA species that might be important for RNA proc...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Lermontova I,Schubert V,Börnke F,Macas J,Schubert I

    更新日期:2007-11-01 00:00:00

  • Tetracycline-dependent activation of an upstream promoter reveals transcriptional interference between tandem genes within T-DNA in tomato.

    abstract::Tomato plants stably expressing the tetR gene were transformed with the beta-glucuronidase (GUS) gene and an le20 antisense construct both under the control of the Triple-Op promoter. Induction of GUS mRNA and le20 antisense RNA in leaves was demonstrated in response to tetracycline. le20 antisense induction was also ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Thompson AJ,Myatt SC

    更新日期:1997-07-01 00:00:00

  • Gene regulation in planta by plant-derived engineered zinc finger protein transcription factors.

    abstract::The ability to modify plant traits is of great commercial potential in agricultural biotechnology. To this end we have engineered plant-based zinc finger protein transcription factors (ZFP TFs) that minimize the use of non-plant DNA sequences. This novel architecture supports the use of tandem arrays of zinc-finger DN...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Holmes-Davis R,Li G,Jamieson AC,Rebar EJ,Liu Q,Kong Y,Case CC,Gregory PD

    更新日期:2005-02-01 00:00:00

  • 14-3-3 proteins and the response to abiotic and biotic stress.

    abstract::14-3-3 proteins function as regulators of a wide range of target proteins in all eukaryotes by effecting direct protein-protein interactions. Primarily, interactions between 14-3-3 proteins and their targets are mediated by phosphorylation at specific sites on the target protein. Hence, interactions with 14-3-3s are s...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审


    authors: Roberts MR,Salinas J,Collinge DB

    更新日期:2002-12-01 00:00:00

  • Genetic integration of DORNRÖSCHEN and DORNRÖSCHEN-LIKE reveals hierarchical interactions in auxin signalling and patterning of the Arabidopsis apical embryo.

    abstract::DORNRÖSCHEN (DRN) and DORNRÖSCHEN-LIKE (DRNL) encode AP2-domain transcription factors, which act redundantly in cotyledon organogenesis. A more detailed genetic study now integrates DRN and DRNL into the CUP-SHAPED COTYLEDON (CUC) regulatory network and places DRN and DRNL differentially within the auxin signalling ne...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Chandler JW,Cole M,Jacobs B,Comelli P,Werr W

    更新日期:2011-02-01 00:00:00

  • Unraveling the functions of type II-prohibitins in Arabidopsis mitochondria.

    abstract::In yeast and mammals, prohibitins (PHBs) are considered as structural proteins that form a scaffold-like structure for interacting with a set of proteins involved in various processes occurring in the mitochondria. The role of PHB in plant mitochondria is poorly understood. In the study, the model organism Arabidopsis...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Piechota J,Bereza M,Sokołowska A,Suszyński K,Lech K,Jańska H

    更新日期:2015-06-01 00:00:00

  • Characteristics of RNA silencing in plants: similarities and differences across kingdoms.

    abstract::RNA silencing is a collective term that encompasses the sequence of events that leads to the targeted degradation of cellular mRNA and thus to the silencing of corresponding gene expression. RNA silencing is initiated after introduction into the host genome of a gene that is homologous to an endogenous gene. Transcrip...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审


    authors: Susi P,Hohkuri M,Wahlroos T,Kilby NJ

    更新日期:2004-01-01 00:00:00

  • Over-expression of a seed specific hevein-like antimicrobial peptide from Pharbitis nil enhances resistance to a fungal pathogen in transgenic tobacco plants.

    abstract::Two hevein-like peptides from the seed of Pharbitis nil, designated Pharbitis nil antimicrobial peptide 1 (Pn-AMP1) and Pn-AMP2, had been purified previously. Both exhibit potent in vitro antifungal activity against a broad spectrum of phytopathogenic fungi. We now report the isolation of two cDNA clones, designated p...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Koo JC,Chun HJ,Park HC,Kim MC,Koo YD,Koo SC,Ok HM,Park SJ,Lee SH,Yun DJ,Lim CO,Bahk JD,Lee SY,Cho MJ

    更新日期:2002-10-01 00:00:00

  • Expression of the genes coding for plastidic acetyl-CoA carboxylase subunits is regulated by a location-sensitive transcription factor binding site.

    abstract::Plastidic acetyl-CoA carboxylase (ACCase) regulates the rate of fatty acid synthesis. This enzyme is composed of biotin carboxyl carrier protein (BCCP), biotin carboxylase (BC), and carboxyltransferase (CT), which consists of α and β subunits. Among these components, CTβ is encoded by the plastidic genome. In Arabidop...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Fukuda N,Ikawa Y,Aoyagi T,Kozaki A

    更新日期:2013-07-01 00:00:00