Abstract:
:Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor composed of NF-YA, NF-YB and NF-YC proteins. In this study, we identified and characterized a gene, GmNFYA3, which encodes the NF-YA subunit of the NF-Y complex in soybeans (Glycine max L.). Real time RT-PCR analysis indicated that GmNFYA3 was induced by abscisic acid (ABA) and abiotic stresses, such as polyethylene glycol, NaCl and cold. Subcellular localization analysis suggested that GmNFYA3 may activate its specific targets in the nucleus. Histochemical β-glucuronidase (GUS) staining revealed that the expression of the GUS gene driven by the GmNFYA3 promoter occurred in various transgenic Arabidopsis tissues. Coexpression in Nicotiana benthamiana and 5' RACE assays indicated that miR169 directs GmNFYA3 mRNA cleavage in vivo. Overexpression of GmNFYA3 resulted in Arabidopsis with reduced leaf water loss and enhanced drought tolerance. In addition, the transgenic Arabidopsis exhibited increased sensitivity to high salinity and exogenous ABA. Moreover, the transcript levels of ABA biosynthesis (ABA1, ABA2), ABA signaling (ABI1, ABI2) and stress-responsive genes, including RD29A and CBF3, were generally higher in GmNFYA3 plants than in wild-type controls under normal conditions. These results suggest that the GmNFYA3 gene functions in positive modulation of drought stress tolerance and has potential applications in molecular breeding to enhance drought tolerance in crops.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Ni Z,Hu Z,Jiang Q,Zhang Hdoi
10.1007/s11103-013-0040-5subject
Has Abstractpub_date
2013-05-01 00:00:00pages
113-29issue
1-2eissn
0167-4412issn
1573-5028journal_volume
82pub_type
杂志文章abstract::Efficient and cost-effective conversion of plant biomass to usable forms of energy requires a thorough understanding of cell wall biosynthesis, modification and degradation. To elucidate these processes, we assessed the expression dynamics during enzymatic removal and regeneration of rice cell walls in suspension cell...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9819-4
更新日期:2011-11-01 00:00:00
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005711720930
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2018-03-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2006-07-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2012-11-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2001-07-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00018558
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1981-03-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00029145
更新日期:1992-10-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1995-10-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1995-01-01 00:00:00
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1995-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-07-01 00:00:00