GmNFYA3, a target gene of miR169, is a positive regulator of plant tolerance to drought stress.

Abstract:

:Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor composed of NF-YA, NF-YB and NF-YC proteins. In this study, we identified and characterized a gene, GmNFYA3, which encodes the NF-YA subunit of the NF-Y complex in soybeans (Glycine max L.). Real time RT-PCR analysis indicated that GmNFYA3 was induced by abscisic acid (ABA) and abiotic stresses, such as polyethylene glycol, NaCl and cold. Subcellular localization analysis suggested that GmNFYA3 may activate its specific targets in the nucleus. Histochemical β-glucuronidase (GUS) staining revealed that the expression of the GUS gene driven by the GmNFYA3 promoter occurred in various transgenic Arabidopsis tissues. Coexpression in Nicotiana benthamiana and 5' RACE assays indicated that miR169 directs GmNFYA3 mRNA cleavage in vivo. Overexpression of GmNFYA3 resulted in Arabidopsis with reduced leaf water loss and enhanced drought tolerance. In addition, the transgenic Arabidopsis exhibited increased sensitivity to high salinity and exogenous ABA. Moreover, the transcript levels of ABA biosynthesis (ABA1, ABA2), ABA signaling (ABI1, ABI2) and stress-responsive genes, including RD29A and CBF3, were generally higher in GmNFYA3 plants than in wild-type controls under normal conditions. These results suggest that the GmNFYA3 gene functions in positive modulation of drought stress tolerance and has potential applications in molecular breeding to enhance drought tolerance in crops.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Ni Z,Hu Z,Jiang Q,Zhang H

doi

10.1007/s11103-013-0040-5

subject

Has Abstract

pub_date

2013-05-01 00:00:00

pages

113-29

issue

1-2

eissn

0167-4412

issn

1573-5028

journal_volume

82

pub_type

杂志文章
  • Fractionation and characterization of polyadenylated RNA from broad bean meristematic root cells.

    abstract::Several populations of polyadenylated RNA from Vicia faba méristematic root cells were fractionated by stepwise thermal elution from poly(U)-Sepharose following sequential phenol extraction. Analysis of these fractions showed that the size of the poly(A) segment could influence this fractionation, but in some cases ot...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00023013

    authors: Trapy G,Favre A,Esnault R

    更新日期:1981-03-01 00:00:00

  • Genome-wide gene expression in an Arabidopsis cell suspension.

    abstract::Plant cell suspension cultures are invaluable models for the study of cellular processes. Here we develop the recently described Arabidopsis suspension culture MM2d as a transcript profiling platform by means of Affymetrix ATH1 microarrays. Analysis of gene expression profiles during normal culture growth, during sync...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/B:PLAN.0000019059.56489.ca

    authors: Menges M,Hennig L,Gruissem W,Murray JA

    更新日期:2003-11-01 00:00:00

  • Two higher molecular weight forms related to ferritin are synthesizedin vitro by RNA from developing cotyledons and leaves ofPhaseolus vulgaris.

    abstract::Using RNA from developing cotyledons and leaves of bean two products were synthesizedin vitro, with Mr 31000 and 31500 which are antigenically related to the subunit of phytoferritin (Mr 26500). The relative abundance of the two products is dependent on the type of tissue from which the RNA was derived. Evidence is pr...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF01578592

    authors: van der Mark F,Klerk H,Bienfait F

    更新日期:1983-11-01 00:00:00

  • Cloning of a cDNA encoding a 3-dehydroquinate synthase from a higher plant, and analysis of the organ-specific and elicitor-induced expression of the corresponding gene.

    abstract::cDNA clones for all enzymes of the prechorismate pathway of higher plants have previously been cloned, with the exception of the second enzyme of the pathway, 3-dehydroquinate synthase. Here we describe the isolation of a cDNA encoding a 3-dehydroquinate synthase from tomato which was identified by complementing a 3-d...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00020607

    authors: Bischoff M,Rösler J,Raesecke HR,Görlach J,Amrhein N,Schmid J

    更新日期:1996-04-01 00:00:00

  • Potato SNF1-related protein kinase: molecular cloning, expression analysis and peptide kinase activity measurements.

    abstract::A polymerase chain reaction product (PKIN503) was amplified from potato (Solanum tuberosum) cv. Désirée using oligonucleotide primers with sequences which are highly conserved in the plant sucrose non-fermenting 1 (SNF1)-related protein kinase gene family. Southern blot analysis showed the presence of 5-10 SNF1-relate...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1005765719873

    authors: Man AL,Purcell PC,Hannappel U,Halford NG

    更新日期:1997-05-01 00:00:00

  • A B-box zinc finger protein, MdBBX10, enhanced salt and drought stresses tolerance in Arabidopsis.

    abstract:KEY MESSAGE:The expression of MdBBX10 was significantly induced by different stresses and ABA treatments. Overexpression of MdBBX10 in Arabidopsis significantly enhanced abiotic stresses tolerance by ABA signalling. The roles of B-box domain(s) containing proteins (BBXs) in regulation of flowering and light morphogenes...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-019-00828-8

    authors: Liu X,Li R,Dai Y,Yuan L,Sun Q,Zhang S,Wang X

    更新日期:2019-03-01 00:00:00

  • The monomeric GTPase RabA2 is required for progression and maintenance of membrane integrity of infection threads during root nodule symbiosis.

    abstract:KEY MESSAGE:Progression of the infection canal that conducts rhizobia to the nodule primordium requires a functional Rab GTPase located in Golgi/trans-Golgi that also participate in root hair polar growth. Common bean (Phaseolus vulgaris) symbiotically associates with its partner Rhizobium etli, resulting in the format...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-016-0581-5

    authors: Dalla Via V,Traubenik S,Rivero C,Aguilar OM,Zanetti ME,Blanco FA

    更新日期:2017-04-01 00:00:00

  • Alternative splicing is required for RCT1-mediated disease resistance in Medicago truncatula.

    abstract::RCT1 is a TIR-NBS-LRR-type resistance (R) gene in Medicago truncatula that confers resistance to multiple races of Colletotrichum trifolii, a hemi-biotrophic fungal pathogen that causes anthracnose disease in Medicago and other closely related legumes. RCT1 undergoes alternative splicing at both coding and 3'-untransl...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-013-0068-6

    authors: Tang F,Yang S,Gao M,Zhu H

    更新日期:2013-07-01 00:00:00

  • Genetic aspects of the organization of legumin genes in pea.

    abstract::We have compared physical and genetic maps of the region around the legJ gene in pea. In this vicinity there are four B-type legumin genes, arranged as two close pairs. The detection of a recombination event within this gene cluster allows the orientation of this group of genes within the surrounding linkage group to ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00038999

    authors: Turner L,Hellens RP,Lee D,Ellis TH

    更新日期:1993-04-01 00:00:00

  • Arabidopsis thaliana contains a large family of germin-like proteins: characterization of cDNA and genomic sequences encoding 12 unique family members.

    abstract::We have identified 39 Arabidopsis thaliana ESTs encoding germin-like proteins (GLPs) and have completely sequenced 25 of these cDNAs. Our analysis demonstrates that the Arabidopsis genome contains a gene family with at least 12 GLP genes. Comparisons with other known germins and germin-like proteins indicate that thes...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006038117130

    authors: Carter C,Graham RA,Thornburg RW

    更新日期:1998-12-01 00:00:00

  • Expression level of the SLG gene is not correlated with the self-incompatibility phenotype in the class II S haplotypes of Brassica oleracea.

    abstract::In Brassica, the S-locus glycoprotein (SLG) gene has been strongly implicated in the self-incompatibility reaction. Several alleles of this locus have been sequenced, and accordingly grouped as class I (corresponding to dominant S-alleles) and class II (recessive). We recently showed that a self-compatible (Sc) line o...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00037027

    authors: Gaude T,Rougier M,Heizmann P,Ockendon DJ,Dumas C

    更新日期:1995-03-01 00:00:00

  • A root-specific condensing enzyme from Lesquerella fendleri that elongates very-long-chain saturated fatty acids.

    abstract::The LfKCS45 gene with a high sequence similarity to known 3-ketoacyl-CoA synthases of the membrane-bound fatty acid elongase was isolated from Lesquerella fendleri. The LfKCS45 gene has a 1464 bp open reading frame without introns, and is predicted to encode a polypeptide of 487 amino acids with an estimated molecular...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-004-6235-z

    authors: Moon H,Chowrira G,Rowland O,Blacklock BJ,Smith MA,Kunst L

    更新日期:2004-12-01 00:00:00

  • Differential screening of mitochondrial cDNA libraries from male-fertile and cytoplasmic male-sterile sugar-beet reveals genome rearrangements at atp6 and atpA loci.

    abstract::As part of a strategy to define differences in genome organization and expression between cytoplasmic male-sterile (CMS) and male-fertile (MF) sugar-beet mitochondria, cDNA libraries from both mitochondrial genotypes were constructed. Preliminary screening with ribosomal RNA gene probes identified candidate cDNA clone...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00024201

    authors: Xue Y,Collin S,Davies DR,Thomas CM

    更新日期:1994-04-01 00:00:00

  • The plant natriuretic peptide receptor is a guanylyl cyclase and enables cGMP-dependent signaling.

    abstract::The functional homologues of vertebrate natriuretic peptides (NPs), the plant natriuretic peptides (PNPs), are a novel class of peptidic hormones that signal via guanosine 3',5'-cyclic monophosphate (cGMP) and systemically affect plant salt and water balance and responses to biotrophic plant pathogens. Although there ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-016-0465-8

    authors: Turek I,Gehring C

    更新日期:2016-06-01 00:00:00

  • A genetic genomics-expression approach reveals components of the molecular mechanisms beyond the cell wall that underlie peach fruit woolliness due to cold storage.

    abstract::Peach fruits subjected to prolonged cold storage (CS) to delay decay and over-ripening often develop a form of chilling injury (CI) called mealiness/woolliness (WLT), a flesh textural disorder characterized by lack of juiciness. Transcript profiles were analyzed after different lengths of CS and subsequent shelf life ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-016-0526-z

    authors: Pons C,Martí C,Forment J,Crisosto CH,Dandekar AM,Granell A

    更新日期:2016-11-01 00:00:00

  • Molecular characterization of glyoxalase-I from a higher plant; upregulation by stress.

    abstract::A cDNA, GLX1, encoding glyoxalase-I was isolated by differential screening of salt-induced genes in tomato. Glyoxalases-I and -II are ubiquitous enzymes whose functions are not clearly understood. They may serve to detoxify methylglyoxal produced from triosephosphates in all cells. The protein encoded by GLX1 shared 4...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00020464

    authors: Espartero J,Sánchez-Aguayo I,Pardo JM

    更新日期:1995-12-01 00:00:00

  • Evidence implying only unprimed RdRP activity during transitive gene silencing in plants.

    abstract::RNA silencing is a sequence-specific RNA degradation mechanism found in most eukaryotes, where small cleavage products (siRNAs) of double stranded RNA (dsRNA) mediate silencing of genes with sequence identity to the dsRNA inducer. In several systems, silencing has been found to spread from the dsRNA inducer sequence i...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-005-7307-4

    authors: Petersen BO,Albrechtsen M

    更新日期:2005-07-01 00:00:00

  • IMPERFECTIVE EXINE FORMATION (IEF) is required for exine formation and male fertility in Arabidopsis.

    abstract:KEY MESSAGE:IEF, a novel plasma plasma membrane protein, is important for exine formation in Arabidopsis. Exine, an important part of pollen wall, is crucial for male fertility. The major component of exine is sporopollenin which are synthesized and secreted by tapetum. Although sporopollenin synthesis has been well st...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-020-01114-8

    authors: Wang K,Zhao X,Pang C,Zhou S,Qian X,Tang N,Yang N,Xu P,Xu X,Gao J

    更新日期:2021-01-22 00:00:00

  • Down-regulation of specific members of the glutamine synthetase gene family in alfalfa by antisense RNA technology.

    abstract::Glutamine synthetase (GS) catalyzes the ATP-dependent condensation of NH3 with glutamate to produce glutamine. In plants GS is an octameric enzyme and is located either in the cytoplasm (GS1) or in the chloroplast (GS2). Two distinct classes of GS1 genes with unique 3'-untranslated region (3'UTR) have been identified ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006099512706

    authors: Temple SJ,Bagga S,Sengupta-Gopalan C

    更新日期:1998-06-01 00:00:00

  • The molecular basis of resistance to the herbicide norflurazon.

    abstract::We have cloned and sequenced a gene, pds, from the cyanobacterium Synechococcus PCC7942 that is responsible for resistance to the bleaching herbicide norflurazon. A point mutation in that gene, leading to an amino acid substitution from valine to glycine in its polypeptide product, was found to confer this resistance....

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00016069

    authors: Chamovitz D,Pecker I,Hirschberg J

    更新日期:1991-06-01 00:00:00

  • The molecular and biochemical basis for varietal variation in sesquiterpene content in melon (Cucumis melo L.) rinds.

    abstract::A combined chemical, biochemical and molecular study was conducted to understand the differential accumulation of volatile sesquiterpenes in melon fruits. Sesquiterpenes were present mainly in the rinds of climacteric varieties, and a great diversity in their composition was found among varieties. Sesquiterpenes were ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-008-9296-6

    authors: Portnoy V,Benyamini Y,Bar E,Harel-Beja R,Gepstein S,Giovannoni JJ,Schaffer AA,Burger J,Tadmor Y,Lewinsohn E,Katzir N

    更新日期:2008-04-01 00:00:00

  • Arabidopsis CBF5 interacts with the H/ACA snoRNP assembly factor NAF1.

    abstract::The conserved protein CBF5, initially regarded as a centromere binding protein in yeast and higher plants, was later found within nucleoli and in Cajal bodies of yeast and metazoa. There, it is assumed to be involved in posttranscriptional pseudouridinylation of various RNA species that might be important for RNA proc...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-007-9226-z

    authors: Lermontova I,Schubert V,Börnke F,Macas J,Schubert I

    更新日期:2007-11-01 00:00:00

  • Tetracycline-dependent activation of an upstream promoter reveals transcriptional interference between tandem genes within T-DNA in tomato.

    abstract::Tomato plants stably expressing the tetR gene were transformed with the beta-glucuronidase (GUS) gene and an le20 antisense construct both under the control of the Triple-Op promoter. Induction of GUS mRNA and le20 antisense RNA in leaves was demonstrated in response to tetracycline. le20 antisense induction was also ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1005888616748

    authors: Thompson AJ,Myatt SC

    更新日期:1997-07-01 00:00:00

  • Gene regulation in planta by plant-derived engineered zinc finger protein transcription factors.

    abstract::The ability to modify plant traits is of great commercial potential in agricultural biotechnology. To this end we have engineered plant-based zinc finger protein transcription factors (ZFP TFs) that minimize the use of non-plant DNA sequences. This novel architecture supports the use of tandem arrays of zinc-finger DN...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-004-7820-x

    authors: Holmes-Davis R,Li G,Jamieson AC,Rebar EJ,Liu Q,Kong Y,Case CC,Gregory PD

    更新日期:2005-02-01 00:00:00

  • 14-3-3 proteins and the response to abiotic and biotic stress.

    abstract::14-3-3 proteins function as regulators of a wide range of target proteins in all eukaryotes by effecting direct protein-protein interactions. Primarily, interactions between 14-3-3 proteins and their targets are mediated by phosphorylation at specific sites on the target protein. Hence, interactions with 14-3-3s are s...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审

    doi:10.1023/a:1021261614491

    authors: Roberts MR,Salinas J,Collinge DB

    更新日期:2002-12-01 00:00:00

  • Genetic integration of DORNRÖSCHEN and DORNRÖSCHEN-LIKE reveals hierarchical interactions in auxin signalling and patterning of the Arabidopsis apical embryo.

    abstract::DORNRÖSCHEN (DRN) and DORNRÖSCHEN-LIKE (DRNL) encode AP2-domain transcription factors, which act redundantly in cotyledon organogenesis. A more detailed genetic study now integrates DRN and DRNL into the CUP-SHAPED COTYLEDON (CUC) regulatory network and places DRN and DRNL differentially within the auxin signalling ne...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-010-9721-5

    authors: Chandler JW,Cole M,Jacobs B,Comelli P,Werr W

    更新日期:2011-02-01 00:00:00

  • Unraveling the functions of type II-prohibitins in Arabidopsis mitochondria.

    abstract::In yeast and mammals, prohibitins (PHBs) are considered as structural proteins that form a scaffold-like structure for interacting with a set of proteins involved in various processes occurring in the mitochondria. The role of PHB in plant mitochondria is poorly understood. In the study, the model organism Arabidopsis...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-015-0320-3

    authors: Piechota J,Bereza M,Sokołowska A,Suszyński K,Lech K,Jańska H

    更新日期:2015-06-01 00:00:00

  • Characteristics of RNA silencing in plants: similarities and differences across kingdoms.

    abstract::RNA silencing is a collective term that encompasses the sequence of events that leads to the targeted degradation of cellular mRNA and thus to the silencing of corresponding gene expression. RNA silencing is initiated after introduction into the host genome of a gene that is homologous to an endogenous gene. Transcrip...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审

    doi:10.1023/B:PLAN.0000028797.63168.a7

    authors: Susi P,Hohkuri M,Wahlroos T,Kilby NJ

    更新日期:2004-01-01 00:00:00

  • Over-expression of a seed specific hevein-like antimicrobial peptide from Pharbitis nil enhances resistance to a fungal pathogen in transgenic tobacco plants.

    abstract::Two hevein-like peptides from the seed of Pharbitis nil, designated Pharbitis nil antimicrobial peptide 1 (Pn-AMP1) and Pn-AMP2, had been purified previously. Both exhibit potent in vitro antifungal activity against a broad spectrum of phytopathogenic fungi. We now report the isolation of two cDNA clones, designated p...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1019864222515

    authors: Koo JC,Chun HJ,Park HC,Kim MC,Koo YD,Koo SC,Ok HM,Park SJ,Lee SH,Yun DJ,Lim CO,Bahk JD,Lee SY,Cho MJ

    更新日期:2002-10-01 00:00:00

  • Expression of the genes coding for plastidic acetyl-CoA carboxylase subunits is regulated by a location-sensitive transcription factor binding site.

    abstract::Plastidic acetyl-CoA carboxylase (ACCase) regulates the rate of fatty acid synthesis. This enzyme is composed of biotin carboxyl carrier protein (BCCP), biotin carboxylase (BC), and carboxyltransferase (CT), which consists of α and β subunits. Among these components, CTβ is encoded by the plastidic genome. In Arabidop...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-013-0075-7

    authors: Fukuda N,Ikawa Y,Aoyagi T,Kozaki A

    更新日期:2013-07-01 00:00:00