Isolation and characterization of a fruit-specific cDNA and the corresponding genomic clone from tomato.

abstract：:Plant responses to abiotic stresses are coordinated by arrays of growth and developmental programs. Phytohormones such as abscisic acid (ABA) and indole-3-acetic acid (IAA) play critical roles in developmental progresses and environmental responses through complex signalling networks. However, crosstalk between the tw...

journal_title：Plant molecular biology

authors： Du H,Wu N,Chang Y,Li X,Xiao J,Xiong L

更新日期：2013-11-01 00:00:00

abstract：:In an attempt to identify domains directly involved in the signal transduction of phytochrome B (phyB), we over-expressed the achromophoric C-terminal half of phyB under control of the CaMV-35S promoter in transgenic Arabidopsis. In three independent transgenic lines, we detected accumulation of the introduced protein...

journal_title：Plant molecular biology

authors： Sakamoto K,Nagatani A

更新日期：1996-08-01 00:00:00

abstract：:The gene coding for a new class of proteins rich in glycine and proline (GPRP) was cloned in Arabidopsis thaliana. In the protein sequence, five amino acids - glycine, proline, alanine, tyrosine and histidine - account for 79.4% of the total composition. The protein has two different glycine-rich domains interrupted b...

journal_title：Plant molecular biology

authors： Marty I,Monfort A,Stiefel V,Ludevid D,Delseny M,Puigdomènech P

更新日期：1996-02-01 00:00:00

abstract：:The 22 kDa auxin-binding proteins in higher plants have received considerable attention as candidates for an auxin receptor. A cDNA clone Ca-ERabp1 of hot pepper (Capsicum annum) was isolated using the oligonucleotides as PCR primers. The cDNA codes for a polypeptide related to the major 22 kDa auxin-binding protein f...

journal_title：Plant molecular biology

authors： Choi SY

更新日期：1996-12-01 00:00:00

abstract：:The gene for the membrane polypeptide D2 has been mapped on the pea (Pisum sativum) chloroplast genome. The nucleotide sequence of the gene and its flanking regions is presented. The only large open reading frame in the sequence codes for a protein of MW 39.5 kD. A potential ribosome binding site is located 6 nucleoti...

journal_title：Plant molecular biology

authors： Rasmussen OF,Bookjans G,Stummann BM,Henningsen KW

更新日期：1984-07-01 00:00:00

abstract：:Peanut (Arachis hypogaea L.), can elicit type I allergy becoming the most common cause of fatal food-induced anaphylactic reactions. Strict avoidance is the only effective means of dealing with this allergy. Ara h 2, a peanut seed storage protein, has been identified as the most potent peanut allergen and is recognize...

journal_title：Plant molecular biology

authors： Ramos ML,Huntley JJ,Maleki SJ,Ozias-Akins P

更新日期：2009-02-01 00:00:00

abstract：:The rice blast resistance gene Pib is a member of the nucleotide binding site (NBS) and leucine-rich repeat (LRR) class of plant disease resistance (R) genes and belongs to a small gene family. We describe here the isolation and characterization of a Pib homologue (PibH8), and extensive investigation of the expression...

journal_title：Plant molecular biology

authors： Wang ZX,Yamanouchi U,Katayose Y,Sasaki T,Yano M

更新日期：2001-11-01 00:00:00

abstract：:In the present study, we utilized TALEN- and CRISPR/Cas9-induced mutations to analyze the promoter of the barley phytase gene HvPAPhy_a. The purpose of the study was dual, validation of the PAPhy_a enzyme as the main contributor of the mature grain phytase activity (MGPA), as well as validating the importance of a spe...

journal_title：Plant molecular biology

authors： Holme IB,Wendt T,Gil-Humanes J,Deleuran LC,Starker CG,Voytas DF,Brinch-Pedersen H

更新日期：2017-09-01 00:00:00

abstract：:A gene family of at least five members encodes the tobacco mitochondrial Rieske Fe-S protein (RISP). To determine whether all five RISPs are translocated to mitochondria, fusion proteins containing the putative presequences of tobacco RISPs and Escherichia coli beta-glucuronidase (GUS) were expressed in transgenic tob...

journal_title：Plant molecular biology

authors： Huang J,Levings CS 3rd

更新日期：1995-11-01 00:00:00

abstract：:The location on the wheat chloroplast DNA map and the nucleotide sequences of the genes coding for tRNA GCC (Gly) (trnG-GCC), tRNA GUC (Asp) (trnD-GUC) and tRNA GCA (Cys) (trnC-GCA) have been determined. These three genes are located in the large single copy region of the chloroplast genome, about half-way between one...

journal_title：Plant molecular biology

authors： Quigley F,Grienenberger JM,Weil JH

更新日期：1985-09-01 00:00:00

abstract：:Left- and right-border repeats, which surround the T-region, contain two conserved regions separated by 5 bp that are not conserved. At the onset of T-DNA processing virD-encoded proteins introduce a nick in the largest of these conserved regions (12 bp) at a specific position in the bottom strand between a guanine an...

journal_title：Plant molecular biology

authors： van Haaren MJ,Sedee NJ,de Boer HA,Schilperoort RA,Hooykaas PJ

更新日期：1989-11-01 00:00:00

abstract：:The sesame 2S albumin (2Salb) promoter was evaluated for its capacity to express the reporter gusA gene encoding β-glucuronidase in transgenic tobacco seeds relative to the soybean fad3C gene promoter element. Results revealed increased expression of gusA gene in tobacco seed tissue when driven by sesame 2S albumin pr...

journal_title：Plant molecular biology

authors： Bhunia RK,Chakraborty A,Kaur R,Gayatri T,Bhattacharyya J,Basu A,Maiti MK,Sen SK

更新日期：2014-11-01 00:00:00

abstract：:Differential screening of cDNA libraries of unfertilized egg cells and in vitro zygotes of maize resulted in the isolation of more than 50 different genes whose expression is up- or down-regulated after in vitro fertilization (IVF). Among these genes, we identified a cDNA encoding the eukaryotic translation initiation...

journal_title：Plant molecular biology

authors： Dresselhaus T,Cordts S,Lörz H

更新日期：1999-03-01 00:00:00

abstract：:In this study, attempt has been made to produce a selected cultivar of tea with low-caffeine content using RNAi technology. The caffeine biosynthetic pathway in tea has been proposed to involve three N-methyltransferases such as xanthosine methyltransferase, 7-N-methylxanthine methyltransferase and 3, 7-dimethylxanthi...

journal_title：Plant molecular biology

authors： Mohanpuria P,Kumar V,Ahuja PS,Yadav SK

更新日期：2011-08-01 00:00:00

abstract：:The identification of some nodule-specific host proteins (nodulins) from common bean (Phaseolus vulgaris L.), a tropical ureide-transporting legume, is described. Particularly, the existence and developmental expression of several abundant nodule-specific transcripts of P. vulgaris are shown, including leghemoglobin, ...

journal_title：Plant molecular biology

authors： Campos F,Padilla J,Vázquez M,Ortega JL,Enríquez C,Sánchez F

更新日期：1987-11-01 00:00:00

abstract：:Pyruvate orthophosphate dikinase (PPDK) is a critical enzyme for C(4) photosynthesis, providing the primary acceptor for fixation of bicarbonate in mesophyll cells. Although first isolated in C(4) plants, it is also present in C(3) species. We report that the single gene encoding PPDK in Arabidopsis thaliana possesses...

journal_title：Plant molecular biology

authors： Parsley K,Hibberd JM

更新日期：2006-10-01 00:00:00

abstract：:The isolation of Brassica napus leaf protoplasts induces reactive oxygen species generation and accumulation in the chloroplasts. An activated isoform of NADPH oxidase-like protein was detected in the protoplasts and the protoplast chloroplasts. The purpose of this study is to define the NADH oxidase-like activities i...

journal_title：Plant molecular biology

authors： Tewari RK,Satoh M,Kado S,Mishina K,Anma M,Enami K,Hanaoka M,Watanabe M

更新日期：2014-12-01 00:00:00

abstract：:The PsbS gene product (PSII-S) which is an integral subunit of photosystem II has recently been reported to be a new type of pigment-binding protein [11]. The chlorophylls of the PSII-S protein exhibit weak excitonic coupling and this protein is stable also in the absence of pigments. Here we investigated the expressi...

journal_title：Plant molecular biology

authors： Adamska I,Funk C,Renger G,Andersson B

更新日期：1996-07-01 00:00:00

abstract：:High molecular weight double-stranded (ds) RNAs have been detected in apparently virus-free French (common) bean Phaseolus vulgaris cv. Black Turtle Soup (BTS). Several other bean cultivars were free of detectable high molecular weight dsRNAs. The dsRNAs have been partially characterized and have homology to the BTS g...

journal_title：Plant molecular biology

authors： Wakarchuk DA,Hamilton RI

更新日期：1985-01-01 00:00:00

abstract：:A method is described for the high frequency transformation of carrot proembryogenic suspension culture cells by a non-oncogenic Ti-plasmid vector (pGV3850::1103) which carried a chimaeric kanamycin resistance gene (nos-NPT-II). Plants were regenerated efficiently from transformed material by somatic embryogenesis in ...

journal_title：Plant molecular biology

authors： Scott RJ,Draper J

更新日期：1987-05-01 00:00:00

abstract：:Repair of the ubiquitous mutagenic lesion 7,8-dihydro-8-oxoguanine (8-oxoG) is initiated in eukaryotes by DNA glycosylases/lyases, such as yeast Ogg1, that do not share significant sequence identity with their prokaryotic counterparts, typified by Escherichia coli MutM (Fpg) protein. The unexpected presence of a funct...

journal_title：Plant molecular biology

authors： García-Ortiz MV,Ariza RR,Roldán-Arjona T

更新日期：2001-12-01 00:00:00

abstract：:Due to an unfortunate turn of events, the first name of the fifth author appeared incorrectly in the original publication and should have read Guangbing. The correct representation of the authors' names and their affiliation is listed here and should be treated as definitive. ...

journal_title：Plant molecular biology

authors： Pan T,Sun X,Liu Y,Li H,Deng G,Lin H,Wang S

更新日期：2018-02-01 00:00:00

abstract：:Type I signal peptidases are a widespread family of enzymes which remove the presequences from proteins translocated across cell membranes, including thylakoid and cytoplasmic membranes of cyanobacteria and thylakoid membranes of chloroplasts. We have cloned and sequenced a signal peptidase gene from the thermophilic ...

journal_title：Plant molecular biology

authors： Packer JC,André D,Howe CJ

更新日期：1995-01-01 00:00:00

abstract：KEY MESSAGE:The design and use of existing VIGS vectors for revealing monocot gene functions are described and potential new vectors are discussed, which may expand their repertoire. Virus induced gene silencing (VIGS) is a method of transient gene silencing in plants, triggered by the use of modified viral vectors. VI...

journal_title：Plant molecular biology

authors： Kant R,Dasgupta I

更新日期：2019-05-01 00:00:00

abstract：:We have identified by differential screening a novel Arabidopsis thaliana gene, called kin1, which is induced at +4 degrees C. The nucleotide sequences of both the genomic clone and the corresponding cDNA were determined. The deduced 6.5 kDa polypeptide has an unusual amino acid composition being rich in alanine, glyc...

journal_title：Plant molecular biology

authors： Kurkela S,Franck M

更新日期：1990-07-01 00:00:00

abstract：:Tomato golden mosaic virus (TGMV), a member of the geminivirus group, has a genome consisting of two DNA molecules designated the A and B components. Both are required for infectivity in healthy plants, although the former has been shown to replicate independently in transgenic plants containing tandem direct repeats ...

journal_title：Plant molecular biology

authors： Sunter G,Gardiner WE,Rushing AE,Rogers SG,Bisaro DM

更新日期：1987-11-01 00:00:00

abstract：:The expression of the alpha' and beta subunit genes of beta-conglycinin is differentially regulated during soybean embryo development. Although both are expressed solely in developing seeds during mid to late stages of embryo development, the alpha' subunit is expressed more highly on a per gene basis, and alpha' subu...

journal_title：Plant molecular biology

authors： Lessard PA,Allen RD,Bernier F,Crispino JD,Fujiwara T,Beachy RN

更新日期：1991-03-01 00:00:00

abstract：:By differential screening of a cDNA library of two-week-old rice seedlings cDNA clones were obtained, corresponding to shoot-specific mRNAs. By sequence analysis two of these clones were found to be rbcS cDNA clones. The mRNA corresponding to a third cDNA clone (COS5) displayed an expression pattern similar to the exp...

journal_title：Plant molecular biology

authors： de Pater S,Hensgens LA,Schilperoort RA

更新日期：1990-09-01 00:00:00

abstract：:A gene encoding the large subunit of ribulose-1,5-bisphosphate carboxylase (Rubisco) was identified on a circular 73 kb DNA from the colourless euglenoid flagellate Astasia longa. The rbcL gene of Astasia extends over 3968 bp. It is a split gene interrupted by seven introns as compared to nine intervening sequences in...

journal_title：Plant molecular biology

authors： Siemeister G,Hachtel W

更新日期：1990-05-01 00:00:00

abstract：:We have isolated and characterized a cDNA from a strawberry fruit subtractive library that shows homology to class-I low-molecular-weight (LMW) heat-shock protein genes from other higher plants. The strawberry cDNA (clone njjs4) was a 779 bp full-length cDNA with a single open reading frame of 468 bp that is expected ...

journal_title：Plant molecular biology

authors： Medina-Escobar N,Cárdenas J,Muñoz-Blanco J,Caballero JL

更新日期：1998-01-01 00:00:00