Abstract:
:Screening of a lambda gt11 cDNA expression library of Euglena gracilis with antibodies directed against histones H2 from maize resulted in the isolation of a full-length cDNA for a histone H2A. The open-reading frame of 408 bp corresponded to a protein of 136 amino acid residues (14 kDa). Despite the presence of a poly(A) tail, which is typical of plant histone mRNA but not of animal histone mRNA, the size of the deduced protein and its percentage of homology were closer to animal histone H2As than to plant or lower eukaryotic histone H2A. Sequence alignment revealed that the Euglena H2A protein was characterized by a shorter C-terminus and a N-terminus which extended 10 residues past the animal H2A. In contrast to other organisms studied, the expression of the Euglena H2A gene appeared to be almost constant during an entire life-cycle and presented no cell-stage-specific expression during development. Similar results are obtained for another histone gene, H3, and for beta-tubulin. Regulation of gene expression at a post-transcriptional level seems to be a general feature of Euglena.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Saint-Guily A,Schantz ML,Schantz Rdoi
10.1007/BF00014447subject
Has Abstractpub_date
1994-03-01 00:00:00pages
941-8issue
6eissn
0167-4412issn
1573-5028journal_volume
24pub_type
杂志文章abstract::Acyl-CoA binding proteins (ACBPs) are small (ca. 10 kDa) highly-conserved cytosolic proteins that bind long-chain acyl-CoAs. A novel cDNA encoding ACBP1, a predicted membrane protein of 24.1 kDa with an acyl-CoA binding protein domain at its carboxy terminus, was cloned from Arabidopsis thaliana. At this domain, ACBP1...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006052108468
更新日期:1998-11-01 00:00:00
abstract::A new heterogeneity of the rDNA spacer of Arabidopsis thaliana, resulting from variation in copy number of the so-called 'C' repeat located downstream of the presumptive polymerase I promoter, is reported. Variation is shown to occur within and between ecotypes. PCR analysis and sequence comparison suggests that the o...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015983
更新日期:1993-06-01 00:00:00
abstract::The isolation of cDNAs is described which encode the complete sequence of a precursor protein for a HSP90 homologue consisting of an N-terminal transit peptide of 5850 Da and a mature protein (cpHSP82) of 82 260 Da, located in the plastids of rye leaves (Secale cereale). Hybridization analysis indicated the presence o...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00049326
更新日期:1996-02-01 00:00:00
abstract:KEY MESSAGE:Lack of structural components results in inflorescence stem bending. Differentially expressed genes involved in lignin and hemicellulose biosynthesis are vital; genes involved in cellulose and glycan biosynthesis are also relevant. An erect inflorescence stem is essential for high-quality cut herbaceous peo...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00945-4
更新日期:2020-02-01 00:00:00
abstract::Glycine betaine (betaine) has the highest cellular osmoprotective efficiency which does not accumulate in most glycophytes. The biosynthetic pathway for betaine in higher plants is derived from the oxidation of low-accumulating metabolite choline that limiting the ability of most plants to produce betaine. Halophilic ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0195-8
更新日期:2014-07-01 00:00:00
abstract::GALACTINOL SYNTHASE is the first committed enzyme in the raffinose biosynthetic pathway. We have previously characterized the maize (Zea mays) GALACTINOL SYNTHASE2 gene (ZmGOLS2) as abiotic stress induced. To further investigate the regulation of ZmGOLS2 gene expression, individual luciferase expression vectors,in whi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-015-0403-1
更新日期:2016-01-01 00:00:00
abstract::SAUR-AC1 is a small-auxin-up-RNA (SAUR) gene of Arabidopsis. Here we demonstrate that the SAUR-AC1 promoter region induces accumulation of a reporter transcript upon treatment with auxin and is preferentially active in elongating hypocotyls and certain other organs and tissues of transgenic Arabidopsis and tobacco pla...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005875300606
更新日期:1997-07-01 00:00:00
abstract::Genomic variation in S1 and S2 homologous sequences, defined as the S regions, were examined in mitochondrial DNAs of 12 normal cytoplasm maize lines collected in the United States. Three genomic variants were detected among the 12 cytoplasms, eight of which were identical to the Wf9 model structure. Hybridization dat...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF01578377
更新日期:1983-07-01 00:00:00
abstract::The R and B genes of maize regulate the anthocyanin biosynthetic pathway and constitute a small gene family whose evolution has been shaped by polyploidization and transposable element activity. To compare the evolution of regulatory genes in the distinct but related genomes of rice and maize, we previously isolated t...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006355510883
更新日期:2000-03-01 00:00:00
abstract::Phaseolin is a glycoprotein that constitutes the major storage protein in bean seeds. The phaseolin gene promoters function in a seed-specific manner. In an attempt to understand if events following transcription of the gene also contribute to the seed-specific accumulation of the phaseolin protein, we studied the eff...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040527
更新日期:1992-09-01 00:00:00
abstract::We have isolated full-size cDNA sequences encoding the photosynthetic isoform of pyruvate orthophosphate dikinase (PPDK) of the C3 plant Flaveria pringlei. The encoded protein shares 96% identical amino acid residues with the C4 isoform of PPDK in the C4 species F. trinervia. The differing amino acid residues are even...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00013761
更新日期:1994-10-01 00:00:00
abstract::Differentiation of the Arabidopsis thaliana (Arabidopsis) seed coat epidermal cells involves pronounced changes highlighted by the synthesis and secretion of copious amounts of dispensable, pectinaceous mucilage followed by a thick cellulosic secondary cell wall. This cell type, therefore, represents an excellent mole...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-012-9984-0
更新日期:2013-01-01 00:00:00
abstract::We have previously shown that two CRE elements situated on a 31 bp region of the cauliflower mosaic virus (CaMV) 35S promoter activate gene expression in the yeast Saccharomyces cerevisiae and are regulated by cAMP. Studies with the yeast transcription factors GCN4, SKO1 and YAP1, which bind CRE-like sequences, showed...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023248
更新日期:1994-05-01 00:00:00
abstract::Gibberellin (GA) 20-oxidase (GA20ox) is a key enzyme that normally catalyzes the penultimate steps in GA biosynthesis. One of the GA20ox genes in rice (Oryza sativa L.), OsGA20ox2 ( SD1 ), is well known as the "Green Revolution gene", and loss-of function mutation in this locus causes semi-dwarfism. Another GA20ox gen...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-1692-y
更新日期:2004-07-01 00:00:00
abstract::14-3-3 proteins form a family of highly conserved proteins with central roles in many eukaryotic signalling networks. In plants, they bind to and activate the plasma membrane H+-ATPase, creating a binding site for the phytotoxin fusicoccin. Barley 14-3-3 transcripts accumulate in the epidermis upon inoculation with th...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1014938417267
更新日期:2002-05-01 00:00:00
abstract::Despite the presence in nature of many functional gene families that contain several to many highly similar sequences, the presence of identical DNA sequence repeats is widely thought to predispose transgene inserts to homology dependent gene silencing (HDGS). The induction of transcriptional gene silencing (TGS) by R...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-5101-y
更新日期:2005-06-01 00:00:00
abstract::Acyl carrier protein (ACP) is a key component of the fatty acid biosynthetic machinery in plants. A 1.4 kb 5' flanking region of a Brassica napus ACP gene (ACP05) was transcriptionally fused to the reporter gene beta-glucuronidase (GUS), and expression of the chimeric gene monitored in transgenic tobacco. GUS activity...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00047719
更新日期:1992-04-01 00:00:00
abstract::Tomato fruit maturation is accompanied by a depolymerization of cell wall pectins which is due to the action of endopolygalacturonase (endoPG) preceded by pectin methylesterase (PE) activity. To investigate the role of endoPG and PE in determining the structure of green bean (Phaseolus vulgaris L.) pectins, these pect...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040831
更新日期:1996-09-01 00:00:00
abstract::We have previously shown that the mitochondrial gene atpA, encoding the alpha subunit of F1 ATP synthase, is associated with DNA rearrangements and nuclear-specific transcript patterns in the male-sterile cytoplasm of Ogura radish. Here we present a detailed characterization of this gene from both the normal (fertile)...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00016123
更新日期:1990-11-01 00:00:00
abstract::Paramutation is a heritable change in gene expression induced by allele interactions. This review summarizes key experiments on three maize loci, which undergo paramutation. Similarities and differences between the phenomenology at the three loci are described. In spite of many differences with respect to the stabilit...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1006499808317
更新日期:2000-06-01 00:00:00
abstract::Nonsense-mediated mRNA decay (NMD) is a eukaryotic quality control system that identifies and eliminates transcripts having a premature translation termination codon (PTC). NMD is also involved in the control of several wild-type mRNAs. The NMD core machinery consists of three highly conserved NMD factors (UPF1, UPF2 ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9726-0
更新日期:2011-02-01 00:00:00
abstract::A cDNA library was constructed from the root tissues of cassava variety Huanan 124 at the root bulking stage. A total of 9,600 cDNA clones from the library were sequenced with single-pass from the 5'-terminus to establish a catalogue of expressed sequence tags (ESTs). Assembly of the resulting EST sequences resulted i...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9698-0
更新日期:2010-12-01 00:00:00
abstract::The expression of beta-amylase genes in rice (Oryza sativa) and its regulation by phytohormones gibberellic acid (GA) and abscisic acid (ABA) were examined. Upon germination beta-amylase is synthesized de novo in aleurone cells and (GA) is not required. Exogenous addition of GA does not enhance the beta-amylase activi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040716
更新日期:1996-08-01 00:00:00
abstract::When subjected to salt stress or drought, some vascular plants such as barley respond with an increased accumulation of the osmoprotectant glycine betaine (betaine), being the last step of betaine synthesis catalyzed by betaine aldehyde dehydrogenase (BADH). We report here cloning and characterization of BADH cDNA fro...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020185
更新日期:1995-01-01 00:00:00
abstract::During maize seed development, endosperm cells synthesize large amounts of storage proteins, alpha-, beta-, and gamma-zeins, which accumulate within endoplasmic reticulum (ER)-derived protein bodies. The absence of lysine in all zein polypeptides results in an imbalance in the amino acid composition of maize seeds. We...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005889314967
更新日期:1997-05-01 00:00:00
abstract::A cDNA encoding the multifunctional cytochrome P450, CYP71E1, involved in the biosynthesis of the cyanogenic glucoside dhurrin from Sorghum bicolor (L.) Moench was isolated. A PCR approach based on three consensus sequences of A-type cytochromes P450- (V/I)KEX(L/F)R, FXPERF, and PFGXGRRXCXG-was applied. Three novel cy...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005915507497
更新日期:1998-02-01 00:00:00
abstract::Heterokont algae such as diatoms, brown seaweeds and the raphidophyte Heterosigma akashiwo acquired their chloroplasts via a secondary endosymbiosis involving a red algal endosymbiont and a eukaryote host, resulting in chloroplasts surrounded by four membranes rather than two. The precursor of a nuclear-encoded thylak...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1023900100803
更新日期:2003-05-01 00:00:00
abstract::It has been known for more than a decade that increasing the gene copy number does not necessarily lead to increased gene activity. Plants have developed efficient mechanisms such as post-transcriptional gene silencing (PTGS) to regulate abnormal gene expression in a sequence-specific fashion. PTGS of (trans)genes can...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1006499832424
更新日期:2000-06-01 00:00:00
abstract::A cDNA library from ethephon-treated cucumber cotyledons (Cucumis sativus L. cv. Poinsett 76) was constructed. Two cDNA clones encoding putative peroxidases were isolated by means of a synthetic probe based on a partial amino acid sequence of a 33 kDa cationic peroxidase that had been previously shown to be induced by...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00016504
更新日期:1990-05-01 00:00:00
abstract::Vascular tissue in plants is unique due to its diverse and dynamic cellular patterns. Signals controlling vascular development have only recently started to emerge through biochemical, genetic, and genomic approaches in several organisms, such as Arabidopsis, Populus, and Zinnia. These signals include hormones (auxin,...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-008-9374-9
更新日期:2009-03-01 00:00:00