The isolation and functional characterisation of a B. napus acyl carrier protein 5' flanking region involved in the regulation of seed storage lipid synthesis.

abstract：:In an attempt to identify domains directly involved in the signal transduction of phytochrome B (phyB), we over-expressed the achromophoric C-terminal half of phyB under control of the CaMV-35S promoter in transgenic Arabidopsis. In three independent transgenic lines, we detected accumulation of the introduced protein...

journal_title：Plant molecular biology

authors： Sakamoto K,Nagatani A

更新日期：1996-08-01 00:00:00

abstract：:Glutelin is the most abundant storage protein in rice, which is expressed specifically in the endosperm of maturing seed. Glutelin is encoded by about 10 genes per haploid genome, which are clearly divided into two subfamilies (GluA and GluB). Most of them are coordinately expressed during seed maturation in spite of ...

journal_title：Plant molecular biology

authors： Takaiwa F,Yamanouchi U,Yoshihara T,Washida H,Tanabe F,Kato A,Yamada K

更新日期：1996-03-01 00:00:00

abstract：:To elucidate the evolutionary relationship between the Spermatophyta, Pteridophyta and Bryophyta, we cloned a fragment of chloroplast DNA from the fern Angiopteris lygodiifolia (Pteridophyta) and determined its nucleotide sequence. The fragment contained the atpB, rbcL, trnR-CCG, dedB and psaI genes. Comparisons of th...

journal_title：Plant molecular biology

authors： Yoshinaga K,Kubota Y,Ishii T,Wada K

更新日期：1992-01-01 00:00:00

abstract：KEY MESSAGE:We identified and cloned the two precursors of miR158 and its target gene in Brassica campestris ssp. chinensis, which both had high relative expression in the inflorescences. Further study revealed that over-expression of miR158 caused reduced pollen varbility, which was caused by the degradation of pollen...

journal_title：Plant molecular biology

authors： Ma Z,Jiang J,Hu Z,Lyu T,Yang Y,Jiang J,Cao J

更新日期：2017-02-01 00:00:00

abstract：:Agrobacterium tumefaciens was used to genetically transform sorghum. Immature embryos of a public (P898012) and a commercial line (PHI391) of sorghum were used as the target explants. The Agrobacterium strain used was LBA4404 carrying a 'Super-binary' vector with a bar gene as a selectable marker for herbicide resista...

journal_title：Plant molecular biology

authors： Zhao ZY,Cai T,Tagliani L,Miller M,Wang N,Pang H,Rudert M,Schroeder S,Hondred D,Seltzer J,Pierce D

更新日期：2000-12-01 00:00:00

abstract：:A chimeric gene consisting of the 5' flanking sequences of a rice glutelin gene (Gt3) linked to the chloramphenicol acetyltransferase (CAT) coding segment was introduced into tobacco via Agrobacterium tumefaciens-mediated transformation. CAT enzyme activity could be detected in extracts from seeds as early as 8 days a...

journal_title：Plant molecular biology

authors： Leisy DJ,Hnilo J,Zhao Y,Okita TW

更新日期：1990-01-01 00:00:00

abstract：:Plants that utilize the highly efficient C4 photosynthetic pathway possess two types of specialized leaf cells, the mesophyll and bundle sheath. In mature leaves of amaranth, a dicotyledonous C4 plant, ribulose 1,5-bisphosphate carboxylase (Rubisco) is localized specifically to the chloroplasts of bundle sheath cells,...

journal_title：Plant molecular biology

authors： Boinski JJ,Wang JL,Xu P,Hotchkiss T,Berry JO

更新日期：1993-06-01 00:00:00

abstract：KEY MESSAGE:MdMYB16 forms homodimers and directly inhibits anthocyanin synthesis via its C-terminal EAR repressor. It weakened the inhibitory effect of MdMYB16 on anthocyanin synthesis when overexpressing MdbHLH33 in callus overexpressing MdMYB16. MdMYB16 could interact with MdbHLH33. Anthocyanins are strong antioxidan...

journal_title：Plant molecular biology

authors： Xu H,Wang N,Liu J,Qu C,Wang Y,Jiang S,Lu N,Wang D,Zhang Z,Chen X

更新日期：2017-05-01 00:00:00

abstract：:Free Air [CO(2)] Enrichment (FACE) allows for plant growth under fully open-air conditions of elevated [CO(2)] at concentrations expected to be reached by mid-century. We used Arabidopsis thaliana ecotypes Col-0, Cvi-0, and WS to analyze changes in gene expression and metabolite profiles of plants grown in "SoyFACE" (...

journal_title：Plant molecular biology

authors： Li P,Sioson A,Mane SP,Ulanov A,Grothaus G,Heath LS,Murali TM,Bohnert HJ,Grene R

更新日期：2006-11-01 00:00:00

abstract：:The promoter region (-309 to +44) of the Brassica napus storage protein gene napA was studied in transgenic tobacco by successive 5' as well as internal deletions fused to the reporter gene GUS (beta-glucuronidase). The expression in the two main tissues of the seed, the endosperm and the embryo, was shown to be diffe...

journal_title：Plant molecular biology

authors： Ellerström M,Stålberg K,Ezcurra I,Rask L

更新日期：1996-12-01 00:00:00

abstract：:Plants perceiving drought activate multiple responses to improve survival, including large-scale alterations in gene expression. This article reports on the roles in the drought response of two Arabidopsis thaliana homeodomain-leucine zipper class I genes; ATHB7 and ATHB12, both strongly induced by water-deficit and a...

journal_title：Plant molecular biology

authors： Valdés AE,Overnäs E,Johansson H,Rada-Iglesias A,Engström P

更新日期：2012-11-01 00:00:00

abstract：:Phaseolin is a glycoprotein that constitutes the major storage protein in bean seeds. The phaseolin gene promoters function in a seed-specific manner. In an attempt to understand if events following transcription of the gene also contribute to the seed-specific accumulation of the phaseolin protein, we studied the eff...

journal_title：Plant molecular biology

authors： Bagga S,Sutton D,Kemp JD,Sengupta-Gopalan C

更新日期：1992-09-01 00:00:00

abstract：:Porphobilinogen deaminase (PBG deaminase) is an early enzyme of the pathway for chlorophyll and heme synthesis. Using degenerate oligonucleotide primers, based on amino acid sequence data for purified PBG deaminase from pea, a fragment was amplified from Arabidopsis genomic DNA by PCR, and then used to isolate both a ...

journal_title：Plant molecular biology

authors： Lim SH,Witty M,Wallace-Cook AD,Ilag LI,Smith AG

更新日期：1994-11-01 00:00:00

abstract：:ATP-binding cassette transporters are involved in the active transport of a wide variety of metabolites in prokaryotes and eukaryotes. One subfamily, the Pleiotropic Drug Resistance (PDR) transporters, or full-size ABCG transporters, are found only in fungi and plants. NtPDR1 was originally identified in Nicotiana tab...

journal_title：Plant molecular biology

authors： Crouzet J,Roland J,Peeters E,Trombik T,Ducos E,Nader J,Boutry M

更新日期：2013-05-01 00:00:00

abstract：KEY MESSAGE:Based on the nature of the proteins that are altered in abundance, we conclude that guard cells switch their energy source from fatty acid metabolism to chloroplast activity, at the onset of dawn. During stomatal opening at dawn, evidence was recently presented for a breakdown and liquidation of stored tria...

journal_title：Plant molecular biology

authors： Geilfus CM,Lan J,Carpentier S

更新日期：2018-12-01 00:00:00

abstract：:We isolated a rice cDNA encoding nucleoside diphosphate kinase (NDK, EC 2.7.4.6). The deduced amino acid sequence of the rice NDK shows highest homology to spinach NDK-I. The rice NDK gene exhibits a strong codon bias (73.8% GC) in the third position of the codon. DNA blot analysis indicated that at least single NDK g...

journal_title：Plant molecular biology

authors： Yano A,Shimazaki T,Kato A,Umeda M,Uchimiya H

更新日期：1993-12-01 00:00:00

abstract：:We have characterized a genomic clone containing the potato pathogenesis-related genes STH-2 and STH-21. The two genes are found 4 kb apart on the same chromosome and their sequences are highly similar. They present the same transcriptional orientation and are both interrupted by a single intron. A chimaeric gene cons...

journal_title：Plant molecular biology

authors： Matton DP,Prescott G,Bertrand C,Camirand A,Brisson N

更新日期：1993-05-01 00:00:00

abstract：:In biosynthesis of octadecanoids and jasmonate (JA), the naturally occurring enantiomer is established in a step catalysed by the gene cloned recently from tomato as a single-copy gene (Ziegler et al., 2000). Based on sequence homology, four full-length cDNAs were isolated from Arabidopsis thaliana ecotype Columbia co...

journal_title：Plant molecular biology

authors： Stenzel I,Hause B,Miersch O,Kurz T,Maucher H,Weichert H,Ziegler J,Feussner I,Wasternack C

更新日期：2003-04-01 00:00:00

abstract：KEY MESSAGE:Overexpression of BoMYB29 gene up-regulates the aliphatic glucosinolate pathway in Brassica oleracea plants increasing the production of the anti-cancer metabolite glucoraphanin, and the toxic and pungent sinigrin. Isothiocyanates, the bio-active hydrolysis products of glucosinolates, naturally produced by ...

journal_title：Plant molecular biology

authors： Zuluaga DL,Graham NS,Klinder A,van Ommen Kloeke AEE,Marcotrigiano AR,Wagstaff C,Verkerk R,Sonnante G,Aarts MGM

更新日期：2019-09-01 00:00:00

abstract：:We have cloned and sequenced a full-length cDNA for uroporphyrinogen decarboxylase (UROD, EC 4.1.1.37) from tobacco (Nicotiana tabacum L.) and a partial cDNA clone from barley (Hordeum vulgare L.). The cDNA of tobacco encodes a protein of 43 kDa, which has 33% overall similarity to UROD sequences determined from other...

journal_title：Plant molecular biology

authors： Mock HP,Trainotti L,Kruse E,Grimm B

更新日期：1995-05-01 00:00:00

abstract：:A three-dimensional model of curculin, a sweet-tasting and taste-modifying protein from the fruits of Curculigo latifolia, was built from the X-ray coordinates of GNA, a mannose-binding lectin from snowdrop (Galanthus nivalis). The three mannose-binding sites present in GNA were found in curculin but are devoid of man...

journal_title：Plant molecular biology

authors： Barre A,Van Damme EJ,Peumans WJ,Rougé P

更新日期：1997-03-01 00:00:00

abstract：:Mitogen-activated protein kinases (MAPKs) constitute one of the most critical signaling components in plants. A typical example is wound-induced protein kinase (WIPK), which functions during pathogen responses in tobacco plants (Nicotiana tabacum). Searching for direct down-stream components, we previously isolated a ...

journal_title：Plant molecular biology

authors： Chung KM,Sano H

更新日期：2007-12-01 00:00:00

abstract：:Plant isoprenoids are derived from two independent pathways, the cytosolic mevalonate pathway and the plastid methylerythritol 4-phosphate (MEP) pathway. We used green fluorescent fusion protein assays to demonstrate that the Arabidopsis MEP pathway enzymes are localized to the chloroplast. We have also characterized ...

journal_title：Plant molecular biology

authors： Hsieh MH,Chang CY,Hsu SJ,Chen JJ

更新日期：2008-04-01 00:00:00

abstract：:Protein folding in vivo is mediated by an array of proteins that act either as 'foldases' or 'molecular chaperones'. Foldases include protein disulfide isomerase and peptidyl prolyl isomerase, which catalyze the rearrangement of disulfide bonds or isomerization of peptide bonds around Pro residues, respectively. Molec...

journal_title：Plant molecular biology

authors： Boston RS,Viitanen PV,Vierling E

更新日期：1996-10-01 00:00:00

abstract：:MYB transcription factors are a large family of proteins involved in the regulation of secondary metabolism and cell shape, the enhancement of disease resistance and the response to different stresses. In this study, the role of TaMYB4 in wheat against biotic and abiotic stresses was investigated. TaMYB4 was cloned fr...

journal_title：Plant molecular biology

authors： Al-Attala MN,Wang X,Abou-Attia MA,Duan X,Kang Z

更新日期：2014-03-01 00:00:00

abstract：:DNA sequence, copy number, expression and phylogenetic relevance of the psbA gene from the abundant marine prokaryote P. marinus CCMP 1375 was analyzed. The 7 amino acids near the C-terminus missing in higher plant and in Prochlorothrix hollandica D1 proteins are present in the derived amino acid sequence. P. marinus ...

journal_title：Plant molecular biology

authors： Hess WR,Weihe A,Loiseaux-de Goër S,Partensky F,Vaulot D

更新日期：1995-03-01 00:00:00

abstract：:RNA editing of T-urf13, a gene specific to the mitochondria of cytoplasmic male-sterile, type-T (cms-T) maize, and an adjacent, cotranscribed gene orf221, have been studied by cDNA sequencing. No editing was detected in 22 cDNA clones. This is the only report of a polypeptide-encoding gene in higher-plant mitochondria...

journal_title：Plant molecular biology

authors： Ward GC,Levings CS 3rd

更新日期：1991-11-01 00:00:00

abstract：:With the completion of genomic sequencing of rice, rice has been firmly established as a model organism for both basic and applied research. The next challenge is to uncover the functions of genes predicted by sequence analysis. Considering the amount of effort and the diversity of disciplines required for functional ...

journal_title：Plant molecular biology

authors： Hirochika H,Guiderdoni E,An G,Hsing YI,Eun MY,Han CD,Upadhyaya N,Ramachandran S,Zhang Q,Pereira A,Sundaresan V,Leung H

更新日期：2004-02-01 00:00:00

abstract：:Iron (Fe) deficiency, a worldwide agricultural problem on calcareous soil with low Fe availability, is also a major human nutritional deficit. Plants induce Fe acquisition systems under conditions of low Fe availability. Previously, we reported that an Fe-deficiency-inducible basic helix-loop-helix (bHLH) transcriptio...

journal_title：Plant molecular biology

authors： Ogo Y,Itai RN,Kobayashi T,Aung MS,Nakanishi H,Nishizawa NK

更新日期：2011-04-01 00:00:00

abstract：:Hevea brasiliensis, being the only source of commercial natural rubber, is an extremely economically important crop. In an effort to facilitate biological, biochemical and molecular research in rubber biosynthesis, here we report the use of next-generation massively parallel sequencing technologies and de novo transcr...

journal_title：Plant molecular biology

authors： Xia Z,Xu H,Zhai J,Li D,Luo H,He C,Huang X

更新日期：2011-10-01 00:00:00