当前位置： SCI文献检索 > PLANT MOLECULAR BIOLOGY期刊下所有文献 > Over-expression of miR158 causes pollen abortion in Brassica campestris ssp. chinensis.

Over-expression of miR158 causes pollen abortion in Brassica campestris ssp. chinensis.

Abstract：

KEY MESSAGE:We identified and cloned the two precursors of miR158 and its target gene in Brassica campestris ssp. chinensis, which both had high relative expression in the inflorescences. Further study revealed that over-expression of miR158 caused reduced pollen varbility, which was caused by the degradation of pollen contents from the binucleate microspore stage. These results first suggest the role of miR158 in pollen development of Brassica campestris ssp. chinensis. MicroRNAs (miRNAs) play crucial roles in many important growth and development processes both in plants and animals by regulating the expression of their target genes via mRNA cleavage or translational repression. In this study, miR158, a Brassicaceae specific miRNA, was functionally characterized with regard to its role in pollen development of non-heading Chinese cabbage (Brassica campestris ssp. chinensis). Two family members of miR158 in B. campestris, namely bra-miR158a1 and bra-miR158a2, and their target gene bra027656, which encodes a pentatricopeptide repeat (PPR) containing protein, were identified. Then, qRT-PCR analysis and GUS-reporter system revealed that both bra-miR158 and its target gene had relatively high expression levels in the inflorescences. Further study revealed that over-expression of miR158 caused reduced pollen varbility and pollen germination ratio, and the degradation of pollen contents from the binucleate microspore stage was also found in those deformed pollen grains, which led to pollen shrinking and collapse in later pollen development stage. These results first shed light on the importance of miR158 in pollen development of Brassica campestris ssp. chinensis.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Ma Z,Jiang J,Hu Z,Lyu T,Yang Y,Jiang J,Cao J

doi

10.1007/s11103-016-0563-7

subject

Has Abstract

pub_date

2017-02-01 00:00:00

pages

313-326

issue

3

eissn

0167-4412

issn

1573-5028

pii

10.1007/s11103-016-0563-7

journal_volume

93

pub_type

杂志文章

相关文献

PLANT MOLECULAR BIOLOGY文献大全
  • Functional analysis of the grapevine paralogs of the SMG7 NMD factor using a heterolog VIGS-based gene depletion-complementation system.

    abstract：:Nonsense-mediated mRNA decay (NMD) is a eukaryotic quality control system that identifies and eliminates transcripts having a premature translation termination codon (PTC). NMD is also involved in the control of several wild-type mRNAs. The NMD core machinery consists of three highly conserved NMD factors (UPF1, UPF2 ...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-010-9726-0

    authors： Benkovics AH,Nyikó T,Mérai Z,Silhavy D,Bisztray GD

    更新日期：2011-02-01 00:00:00

  • mRNA transcription profile of potato (Solanum tuberosum L.) exposed to ultrasound during different stages of in vitro plantlet development.

    abstract：KEY MESSAGE:In response to an ultrasound pulse, several hundred DEGs, including in response to stress, were up- or down-regulated in in vitro potato plantlets. Despite this abiotic stress, plantlets survived. Ultrasound (US) can influence plant growth and development. To better understand the genetic mechanism underlyi...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-019-00876-0

    authors： Dobránszki J,Hidvégi N,Gulyás A,Teixeira da Silva JA

    更新日期：2019-07-01 00:00:00

  • Porphobilinogen deaminase is encoded by a single gene in Arabidopsis thaliana and is targeted to the chloroplasts.

    abstract：:Porphobilinogen deaminase (PBG deaminase) is an early enzyme of the pathway for chlorophyll and heme synthesis. Using degenerate oligonucleotide primers, based on amino acid sequence data for purified PBG deaminase from pea, a fragment was amplified from Arabidopsis genomic DNA by PCR, and then used to isolate both a ...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00028854

    authors： Lim SH,Witty M,Wallace-Cook AD,Ilag LI,Smith AG

    更新日期：1994-11-01 00:00:00

  • Light-modulated abundance of an mRNA encoding a calmodulin-regulated, chromatin-associated NTPase in pea.

    abstract：:A CDNA encoding a 47 kDa nucleoside triphosphatase (NTPase) that is associated with the chromatin of pea nuclei has been cloned and sequenced. The translated sequence of the cDNA includes several domains predicted by known biochemical properties of the enzyme, including five motifs characteristic of the ATP-binding do...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00017808

    authors： Hsieh HL,Tong CG,Thomas C,Roux SJ

    更新日期：1996-01-01 00:00:00

  • A high-density genetic map of Sorghum bicolor (L.) Moench based on 2926 AFLP, RFLP and SSR markers.

    abstract：:Using AFLP technology and a recombinant inbred line population derived from the sorghum cross of BTx623 x IS3620C, a high-density genetic map of the sorghum genome was constructed. The 1713 cM map encompassed 2926 loci distributed on ten linkage groups; 2454 of those loci are AFLP products generated from either the Ec...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1023/a:1014831302392

    authors： Menz MA,Klein RR,Mullet JE,Obert JA,Unruh NC,Klein PE

    更新日期：2002-03-01 00:00:00

  • Transcriptome analysis approaches for the isolation of trichome-specific genes from the medicinal plant Cistus creticus subsp. creticus.

    abstract：:Cistus creticus subsp. creticus is a plant of intrinsic scientific interest due to the distinctive pharmaceutical properties of its resin. Labdane-type diterpenes, the main constituents of the resin, exhibit considerable antibacterial and cytotoxic activities. In this study chemical analysis of isolated trichomes from...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-008-9399-0

    authors： Falara V,Fotopoulos V,Margaritis T,Anastasaki T,Pateraki I,Bosabalidis AM,Kafetzopoulos D,Demetzos C,Pichersky E,Kanellis AK

    更新日期：2008-12-01 00:00:00

  • Isolation and characterization of P-type H(+)-ATPase genes from potato.

    abstract：:H(+)-ATPase cDNAs were identified in a potato leaf library using an Arabidopsis gene as a probe. Based on their sequences, the clones could be grouped into at least two classes. A similar classification was obtained from the analysis of sequence data from four tobacco genes. Both potato genes are expressed in all tiss...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00028864

    authors： Harms K,Wöhner RV,Schulz B,Frommer WB

    更新日期：1994-11-01 00:00:00

  • The isolation and functional characterisation of a B. napus acyl carrier protein 5' flanking region involved in the regulation of seed storage lipid synthesis.

    abstract：:Acyl carrier protein (ACP) is a key component of the fatty acid biosynthetic machinery in plants. A 1.4 kb 5' flanking region of a Brassica napus ACP gene (ACP05) was transcriptionally fused to the reporter gene beta-glucuronidase (GUS), and expression of the chimeric gene monitored in transgenic tobacco. GUS activity...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00047719

    authors： de Silva J,Robinson SJ,Safford R

    更新日期：1992-04-01 00:00:00

  • The SELF-PRUNING gene family in tomato.

    abstract：:The SELF PRUNING (SP) gene controls the regularity of the vegetative-reproductive switch along the compound shoot of tomato and thus conditions the 'determinate' (sp/sp) and 'indeterminate' (SP_) growth habits of the plant. SP is a developmental regulator which is homologous to CENTRORADIALIS (CEN) from Antirrhinum an...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1023/b:plan.0000004333.96451.11

    authors： Carmel-Goren L,Liu YS,Lifschitz E,Zamir D

    更新日期：2003-08-01 00:00:00

  • Floral expression of a gene encoding an E2-related ubiquitin-conjugating protein from Arabidopsis thaliana.

    abstract：:An Arabidopsis thaliana gene (UBC6) encoding a homologue to ubiquitin-conjugating enzymes has been isolated which is capable of encoding a protein of 183 amino acids of ca. 21 kDa. Northern analysis indicates that the gene is expressed in flowers, seeds and, to a somewhat lesser extent, in 10-day seedlings but not in ...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00039553

    authors： Watts FZ,Butt N,Layfield P,Machuka J,Burke JF,Moore AL

    更新日期：1994-10-01 00:00:00

  • RAP2.6L and jasmonic acid-responsive genes are expressed upon Arabidopsis hypocotyl grafting but are not needed for cell proliferation related to healing.

    abstract：KEY MESSAGE:Jasmonic acid and RAP2.6L are induced upon wounding but are not involved in cell proliferation during healing in Arabidopsis hypocotyls. Plants produce jasmonic acid in response to wounding, but its role in healing, if any, has not been determined. Previously, the jasmonic acid-induced transcription factor,...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-018-0702-4

    authors： Matsuoka K,Yanagi R,Yumoto E,Yokota T,Yamane H,Satoh S,Asahina M

    更新日期：2018-04-01 00:00:00

  • The chimeric cyclic nucleotide-gated ion channel ATCNGC11/12 constitutively induces programmed cell death in a Ca2+ dependent manner.

    abstract：:The hypersensitive response (HR) involves programmed cell death (PCD) in response to pathogen infection. To investigate the pathogen resistance signaling pathway, we previously identified the Arabidopsis mutant cpr22, which displays constitutive activation of multiple defense responses including HR like cell death. Th...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-007-9239-7

    authors： Urquhart W,Gunawardena AH,Moeder W,Ali R,Berkowitz GA,Yoshioka K

    更新日期：2007-12-01 00:00:00

  • Extensive alterations in DNA methylation and transcription in rice caused by introgression from Zizania latifolia.

    abstract：:It has been demonstrated that insertion of foreign DNA into mammalian genome can profoundly alter the patterns of DNA methylation and transcription of the host genome. Introgression of alien DNA into plant genomes through sexual crossing and genetic engineering are commonly used in breeding, but it is not known if pla...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1023/B:PLAN.0000038270.48326.7a

    authors： Liu Z,Wang Y,Shen Y,Guo W,Hao S,Liu B

    更新日期：2004-03-01 00:00:00

  • Identification and characterization of a hypoallergenic ortholog of Ara h 2.01.

    abstract：:Peanut (Arachis hypogaea L.), can elicit type I allergy becoming the most common cause of fatal food-induced anaphylactic reactions. Strict avoidance is the only effective means of dealing with this allergy. Ara h 2, a peanut seed storage protein, has been identified as the most potent peanut allergen and is recognize...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-008-9428-z

    authors： Ramos ML,Huntley JJ,Maleki SJ,Ozias-Akins P

    更新日期：2009-02-01 00:00:00

  • A genetic genomics-expression approach reveals components of the molecular mechanisms beyond the cell wall that underlie peach fruit woolliness due to cold storage.

    abstract：:Peach fruits subjected to prolonged cold storage (CS) to delay decay and over-ripening often develop a form of chilling injury (CI) called mealiness/woolliness (WLT), a flesh textural disorder characterized by lack of juiciness. Transcript profiles were analyzed after different lengths of CS and subsequent shelf life ...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-016-0526-z

    authors： Pons C,Martí C,Forment J,Crisosto CH,Dandekar AM,Granell A

    更新日期：2016-11-01 00:00:00

  • Agrobacterium-mediated sorghum transformation.

    abstract：:Agrobacterium tumefaciens was used to genetically transform sorghum. Immature embryos of a public (P898012) and a commercial line (PHI391) of sorghum were used as the target explants. The Agrobacterium strain used was LBA4404 carrying a 'Super-binary' vector with a bar gene as a selectable marker for herbicide resista...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1023/a:1026507517182

    authors： Zhao ZY,Cai T,Tagliani L,Miller M,Wang N,Pang H,Rudert M,Schroeder S,Hondred D,Seltzer J,Pierce D

    更新日期：2000-12-01 00:00:00

  • OsbZIP71, a bZIP transcription factor, confers salinity and drought tolerance in rice.

    abstract：:The bZIP transcription factor (TF) family plays an important role in the abscisic acid (ABA) signaling pathway of abiotic stress in plants. We here report the cloning and characterization of OsbZIP71, which encodes a rice bZIP TF. Functional analysis showed that OsbZIP71 is a nuclear-localized protein that specificall...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-013-0115-3

    authors： Liu C,Mao B,Ou S,Wang W,Liu L,Wu Y,Chu C,Wang X

    更新日期：2014-01-01 00:00:00

  • Correction to: Differential expression of major genes involved in the biosynthesis of aliphatic glucosinolates in intergeneric Baemoochae (Brassicaceae) and its parents during development.

    abstract：:Due to an unfortunate turn of events, an incorrect note was provided in the original publication as it should have read. ...

    journal_title：Plant molecular biology

    pub_type： 已发布勘误

    doi：10.1007/s11103-020-00970-8

    authors： Nugroho ABD,Han N,Pervitasari AN,Kim DH,Kim J

    更新日期：2020-03-01 00:00:00

  • Blue light is required for survival of the tomato phytochrome-deficient aurea mutant and the expression of four nuclear genes coding for plastidic proteins.

    abstract：:When dark-grown aurea mutant tomato seedlings which lack more than 95% of the phytochrome present in isogenic wild-type seedlings are kept in white or blue light, four nuclear-encoded transcripts coding for plastidic proteins (the light-harvesting chlorophyll a/b-binding protein of photosystem I and II [cab-PSII], pla...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00020560

    authors： Oelmüller R,Kendrick RE

    更新日期：1991-02-01 00:00:00

  • Nucleotide sequence of atpB, rbcL, trnR, dedB and psaI chloroplast genes from a fern Angiopteris lygodiifolia: a possible emergence of Spermatophyta lineage before the separation of Bryophyta and Pteridophyta.

    abstract：:To elucidate the evolutionary relationship between the Spermatophyta, Pteridophyta and Bryophyta, we cloned a fragment of chloroplast DNA from the fern Angiopteris lygodiifolia (Pteridophyta) and determined its nucleotide sequence. The fragment contained the atpB, rbcL, trnR-CCG, dedB and psaI genes. Comparisons of th...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00018458

    authors： Yoshinaga K,Kubota Y,Ishii T,Wada K

    更新日期：1992-01-01 00:00:00

  • TGA3 is a distinct member of the TGA family of bZIP transcription factors in Arabidopsis thaliana.

    abstract：:TGA1a is a well-characterized transcription factor that may mediate the root-specific and auxin-responsive expression of some plant genes. In tobacco, Southern blot and genomic cloning analyses have shown that TGA1a consists of at least four closely related genes. Since TGA1a belongs to the bZIP class of transcription...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00024193

    authors： Miao ZH,Liu X,Lam E

    更新日期：1994-04-01 00:00:00

  • Cyanidin based anthocyanin biosynthesis in orange carrot is restored by expression of AmRosea1 and AmDelila, MYB and bHLH transcription factors.

    abstract：KEY MESSAGE:The simultaneous expression of AmRosea1 and AmDelila transcription factors from snapdragon can activate the anthocyanin pathway in orange carrots, leading to the synthesis and accumulation of anthocyanins in the taproots. Anthocyanins are phenolic compounds produced in various parts of plants. They are used...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-020-01002-1

    authors： Sharma S,Holme IB,Dionisio G,Kodama M,Dzhanfezova T,Joernsgaard B,Brinch-Pedersen H

    更新日期：2020-07-01 00:00:00

  • Secondary xylem-specific expression of caffeoyl-coenzyme A 3-O-methyltransferase plays an important role in the methylation pathway associated with lignin biosynthesis in loblolly pine.

    abstract：:Two types of structurally distinct O-methyltransferases mediate the methylation of hydroxylated monomeric lignin precursors in angiosperms. Caffeate 3-O-methyltransferase (COMT; EC 2.1.1.68) methylates the free acids and caffeoyl CoA 3-O-methyltransferase (CCoAOMT; EC 2.1.1.104) methylates coenzyme A esters. Recently,...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1023/a:1006244325250

    authors： Li L,Osakabe Y,Joshi CP,Chiang VL

    更新日期：1999-07-01 00:00:00

  • Characterization of the LI818 polypeptide from the green unicellular alga Chlamydomonas reinhardtii.

    abstract：:The LI818 gene from Chlamydomonas encodes a polypeptide that is related to the chlorophyll a/b-binding proteins (CAB) of higher plants and green algae. However, despite this relatedness, LI818 gene expression is not coordinated with that of cab genes and is regulated differently by light, suggesting a different role f...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1023/a:1006340308077

    authors： Richard C,Ouellet H,Guertin M

    更新日期：2000-01-01 00:00:00

  • Comparative analysis of chrysanthemum transcriptome in response to three RNA viruses: Cucumber mosaic virus, Tomato spotted wilt virus and Potato virus X.

    abstract：:The chrysanthemum is one of popular flowers in the world and a host for several viruses. So far, molecular interaction studies between the chrysanthemum and viruses are limited. In this study, we carried out a transcriptome analysis of chrysanthemum in response to three different viruses including Cucumber mosaic viru...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-015-0317-y

    authors： Choi H,Jo Y,Lian S,Jo KM,Chu H,Yoon JY,Choi SK,Kim KH,Cho WK

    更新日期：2015-06-01 00:00:00

  • The highly expressed tapetum-specific A9 gene is not required for male fertility in Brassica napus.

    abstract：:An antisense approach was used to attempt to determine the function of the highly abundant, tapetum-specific A9 transcript in microsporogenesis. A Brassica napus A9 cDNA clone was linked in sense and antisense orientations to the Arabidopsis thaliana A9 promoter and the resulting chimaeric genes introduced into B. nap...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00040577

    authors： Turgut K,Barsby T,Craze M,Freeman J,Hodge R,Paul W,Scott R

    更新日期：1994-01-01 00:00:00

  • Contrasting evolutionary patterns and target specificities among three Tourist-like MITE families in the maize genome.

    abstract：:Miniature inverted-repeat transposable elements (MITEs) are short, non autonomous DNA elements that are widespread and abundant in plant genomes. The high sequence and size conservation observed in many MITE families suggest that they have spread recently throughout their respective host genomes. Here we present a mai...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-009-9511-0

    authors： Zerjal T,Joets J,Alix K,Grandbastien MA,Tenaillon MI

    更新日期：2009-09-01 00:00:00

  • Roles of R2R3-MYB transcription factors in transcriptional regulation of anthocyanin biosynthesis in horticultural plants.

    abstract：KEY MESSAGE:This review contains functional roles of MYB transcription factors in the transcriptional regulation of anthocyanin biosynthesis in horticultural plants. This review describes potential uses of MYB TFs as tools for metabolic engineering for anthocyanin production. Anthocyanins (ranging from red to blue) are...

    journal_title：Plant molecular biology

    pub_type： 杂志文章,评审

    doi：10.1007/s11103-018-0771-4

    authors： Naing AH,Kim CK

    更新日期：2018-09-01 00:00:00

  • Induction of chilling resistance by water stress, and cDNA sequence analysis and expression of water stress-regulated genes in rice.

    abstract：:Exposure of seedlings of a chilling-sensitive variety of rice (Oryza sativa L. cv. Wasetoittu) to water stress (0.5 M mannitol, 30 min) at room temperature induced a degree of chilling resistance. No such resistance was induced by exogenous abscisic acid (ABA) application (10 microM, 60 min). Upon short-term water str...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00039544

    authors： Takahashi R,Joshee N,Kitagawa Y

    更新日期：1994-10-01 00:00:00

  • The molecular and biochemical basis for varietal variation in sesquiterpene content in melon (Cucumis melo L.) rinds.

    abstract：:A combined chemical, biochemical and molecular study was conducted to understand the differential accumulation of volatile sesquiterpenes in melon fruits. Sesquiterpenes were present mainly in the rinds of climacteric varieties, and a great diversity in their composition was found among varieties. Sesquiterpenes were ...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-008-9296-6

    authors： Portnoy V,Benyamini Y,Bar E,Harel-Beja R,Gepstein S,Giovannoni JJ,Schaffer AA,Burger J,Tadmor Y,Lewinsohn E,Katzir N

    更新日期：2008-04-01 00:00:00