Identification and characterization of a hypoallergenic ortholog of Ara h 2.01.

abstract：:Transgenic Arabidopsis thaliana plants constitutively expressing Agrobacterium tumefaciens tryptophan monooxygenase (iaaM) were obtained and characterized. Arabidopsis plants expressing iaaM have up to 4-fold higher levels of free indole-3-acetic acid (IAA) and display increased hypocotyl elongation in the light. This...

journal_title：Plant molecular biology

authors： Romano CP,Robson PR,Smith H,Estelle M,Klee H

更新日期：1995-03-01 00:00:00

abstract：:The coding sequence for FLP recombinase, originally from the 2 mu plasmid of Saccharomyces cerevisiae, was introduced into Arabidopsis behind the cauliflower mosaic virus 35S promoter. FLP activity was monitored by the glucuronidase activity resulting from inversion of an antisense-oriented GUS reporter gene flanked b...

journal_title：Plant molecular biology

authors： Sonti RV,Tissier AF,Wong D,Viret JF,Signer ER

更新日期：1995-09-01 00:00:00

abstract：:Cyclin-dependent protein kinases are involved in many crucial cellular processes and aspects of plant growth and development, but their precise roles in abiotic stress responses are largely unknown. Here, Arabidopsis thaliana CYCLIN-DEPENDENT KINASE G2 (CDKG2) was shown to act as a negative regulator of the salinity s...

journal_title：Plant molecular biology

authors： Ma X,Qiao Z,Chen D,Yang W,Zhou R,Zhang W,Wang M

更新日期：2015-06-01 00:00:00

abstract：:Complex signal transduction pathways underlie the myriad plant responses to attack by pathogens. Ca(2+) is a universal second messenger in eukaryotes that modulates various signal transduction pathways through stimulus-specific changes in its intracellular concentration. Ca(2+)-binding proteins such as calmodulin (CaM...

journal_title：Plant molecular biology

authors： Chiasson D,Ekengren SK,Martin GB,Dobney SL,Snedden WA

更新日期：2005-08-01 00:00:00

abstract：:To study the pattern of gene regulation of the plastid chaperonin 60 beta gene family a chimaeric gene was constructed fusing the 5'-flanking region of the chaperonin 60 beta B3 gene to the beta-glucuronidase reporter gene. Histochemical and fluorometric analysis of the GUS activity present in transgenic plants harbou...

journal_title：Plant molecular biology

authors： Zabaleta E,Assad N,Oropeza A,Salerno G,Herrera-Estrella L

更新日期：1994-01-01 00:00:00

abstract：:The sequence of the S-adenosyl-L-methionine:trans-caffeoyl-CoA O-methyltransferase (CCoAOMT, EC2.1.1.104) gene, including the 5'-flanking region of 5 kb, was determined from parsley (Petroselinum crispum) plants. The enzyme appears to be encoded by one or two genes, and the ORF is arranged in five exons spaced by intr...

journal_title：Plant molecular biology

authors： Grimmig B,Matern U

更新日期：1997-01-01 00:00:00

abstract：:Homologous recombination is an essential process for the maintenance and variability of the genome. In eukaryotes, the Rad52 epistasis group proteins serve the main role for meiotic recombination and/or homologous recombinational repair. Rad51-like proteins, such as Rad55 and Rad57 in yeast, play a role in assembly or...

journal_title：Plant molecular biology

authors： Osakabe K,Yoshioka T,Ichikawa H,Toki S

更新日期：2002-09-01 00:00:00

abstract：:Protein array technology has emerged as a new tool to enable ordered screening of proteins for expression and molecular interactions in high throughput. Besides classical solid-phase substrates, such as micro-titre plates and membrane filters, protein arrays have recently been devised with chip-sized supports. Several...

journal_title：Plant molecular biology

authors： Kersten B,Feilner T,Kramer A,Wehrmeyer S,Possling A,Witt I,Zanor MI,Stracke R,Lueking A,Kreutzberger J,Lehrach H,Cahilll DJ

更新日期：2003-07-01 00:00:00

abstract：:The formation and the development of the floral organs require an intercalate expression of organ-specific genes. At the same time, meristem-specific genes are repressed to complete the differentiation of the organs in the floral whorls. In an Arabidopsis activation tagging population, a mutant affected in inflorescen...

journal_title：Plant molecular biology

authors： Chalfun-Junior A,Franken J,Mes JJ,Marsch-Martinez N,Pereira A,Angenent GC

更新日期：2005-03-01 00:00:00

abstract：:Exocarp color of sand pear is an important trait for the fruit production and has caused our concern for a long time. Our previous study explored the different expression genes between the two genotypes contrasting for exocarp color, which indicated the different suberin, cutin, wax and lignin biosynthesis between the...

journal_title：Plant molecular biology

authors： Wang YZ,Zhang S,Dai MS,Shi ZB

更新日期：2014-05-01 00:00:00

abstract：:A cDNA library was made from poly(A)+ RNA isolated from developing Douglas fir (Pseudotsuga menziesii) embryo and megagametophytic tissue, and the cDNA clones were identified by immunoscreening with polyclonal antiserum against the crystalloid storage protein complex of Douglas fir. The nucleotide sequence of the long...

journal_title：Plant molecular biology

authors： Leal I,Misra S

更新日期：1993-02-01 00:00:00

abstract：:Phosphorus (P) is one of the essential nutrient elements for plant development. In this work, BnPht1;4 gene, encoding a phosphate transporter of PHT1 family, was isolated from Brassica napus. BnPht1;4 possesses the major characteristic of PHT1 high-affinity Pi transporters in plants, such as plasma-membrane localizati...

journal_title：Plant molecular biology

authors： Ren F,Zhao CZ,Liu CS,Huang KL,Guo QQ,Chang LL,Xiong H,Li XB

更新日期：2014-12-01 00:00:00

abstract：:APETALA2 (AP2) transcription factors (TFs) play very important roles in plant growth and development and in defense response. Here, we report functional characterization of four AP2 TF family genes [(RAP2.6 (At1g43160), RAP2.6L (At5g13330), DREB 26 (At1g21910) and DREB19 (At2g38340)] that were identified among NaCl in...

journal_title：Plant molecular biology

authors： Krishnaswamy S,Verma S,Rahman MH,Kav NN

更新日期：2011-01-01 00:00:00

abstract：:The full-length nucleotide sequence of a barley (Hordeum vulgare L.) leaf mRNA, found to increase rapidly in amount during infection attempts by the powdery mildew fungus (Erysiphe graminis DC. ex Mérat), is reported. The mRNA encodes a polypeptide of 809 amino acid residues which, by sequence comparison, was identifi...

journal_title：Plant molecular biology

authors： Walther-Larsen H,Brandt J,Collinge DB,Thordal-Christensen H

更新日期：1993-03-01 00:00:00

abstract：:The molecular mechanism of signal transduction pathways which mediate the action of phytohormones are poorly understood. Recently, we and others have shown that the as -1 type cis-acting elements can respond to auxin and salicylic acid, two well-characterized signaling molecules in plants. In the present work, we have...

journal_title：Plant molecular biology

authors： Xiang C,Miao ZH,Lam E

更新日期：1996-11-01 00:00:00

abstract：:A polymerase chain reaction product (PKIN503) was amplified from potato (Solanum tuberosum) cv. Désirée using oligonucleotide primers with sequences which are highly conserved in the plant sucrose non-fermenting 1 (SNF1)-related protein kinase gene family. Southern blot analysis showed the presence of 5-10 SNF1-relate...

journal_title：Plant molecular biology

authors： Man AL,Purcell PC,Hannappel U,Halford NG

更新日期：1997-05-01 00:00:00

abstract：:Despite the presence in nature of many functional gene families that contain several to many highly similar sequences, the presence of identical DNA sequence repeats is widely thought to predispose transgene inserts to homology dependent gene silencing (HDGS). The induction of transcriptional gene silencing (TGS) by R...

journal_title：Plant molecular biology

authors： Yang G,Lee YH,Jiang Y,Kumpatla SP,Hall TC

更新日期：2005-06-01 00:00:00

abstract：:We have constructed a yeast artificial chromosome (YAC) library using high-molecular-weight DNA prepared from agarose-embedded leaf protoplasts of the maize inbred line UE95. This library contains 79,000 clones with an average insert size of 145 kb and should therefore represent approximately three haploid genome equi...

journal_title：Plant molecular biology

authors： Edwards KJ,Thompson H,Edwards D,de Saizieu A,Sparks C,Thompson JA,Greenland AJ,Eyers M,Schuch W

更新日期：1992-05-01 00:00:00

abstract：:We describe a non-LTR retrotransposon family,BvL, of the long interspersed nuclear elements L1 clade isolated from sugar beet (Beta vulgaris). Characteristic molecular domains of three full-length BvL elements were determined in detail, showing that coding sequences are interrupted and most likely non-functionally. In...

journal_title：Plant molecular biology

authors： Wenke T,Holtgräwe D,Horn AV,Weisshaar B,Schmidt T

更新日期：2009-12-01 00:00:00

abstract：KEY MESSAGE:During abiotic stress low abundant amino acids are not synthesized but they accumulate due to increased protein turnover under conditions inducing carbohydrate starvation (dehydration, salt stress, darkness) and are degraded. Metabolic adaptation is crucial for abiotic stress resistance in plants, and accum...

journal_title：Plant molecular biology

authors： Hildebrandt TM

更新日期：2018-09-01 00:00:00

abstract：:Ustilago maydis killer toxins are small polypeptides (7-14 kDa) which kill susceptible cells of closely related fungal species. The KP4 toxin is a single polypeptide subunit with a molecular weight of 11.1 kDa. In this work, a transgenic tobacco plant was constructed which secretes the KP4 toxin at a high level. The K...

journal_title：Plant molecular biology

authors： Park CM,Berry JO,Bruenn JA

更新日期：1996-01-01 00:00:00

abstract：:We have cloned and sequenced a gene, pds, from the cyanobacterium Synechococcus PCC7942 that is responsible for resistance to the bleaching herbicide norflurazon. A point mutation in that gene, leading to an amino acid substitution from valine to glycine in its polypeptide product, was found to confer this resistance....

journal_title：Plant molecular biology

authors： Chamovitz D,Pecker I,Hirschberg J

更新日期：1991-06-01 00:00:00

abstract：:In order to analyse the cruciferin gene family in radish a cDNA library was screened either with heterologous rapeseed probes or by differential screening and sequencing. We could identify six partial cDNA clones belonging to two different groups of cruciferin genes which do not cross-hybridize, and probably three dis...

journal_title：Plant molecular biology

authors： Depigny-This D,Raynal M,Aspart L,Delseny M,Grellet F

更新日期：1992-11-01 00:00:00

abstract：:The gene for the membrane polypeptide D2 has been mapped on the pea (Pisum sativum) chloroplast genome. The nucleotide sequence of the gene and its flanking regions is presented. The only large open reading frame in the sequence codes for a protein of MW 39.5 kD. A potential ribosome binding site is located 6 nucleoti...

journal_title：Plant molecular biology

authors： Rasmussen OF,Bookjans G,Stummann BM,Henningsen KW

更新日期：1984-07-01 00:00:00

abstract：:Histone acetylation is modulated through the action of histone acetyltransferase and deacetylase, which play key roles in the regulation of eukaryotic gene expression. We have screened the expressed sequence tag database with the yeast histone deacetylase RPD3 sequence and identified two Arabidopsis homologues, AtRPD3...

journal_title：Plant molecular biology

authors： Wu K,Malik K,Tian L,Brown D,Miki B

更新日期：2000-09-01 00:00:00

abstract：:Glycinebetaine is an important osmoprotectant in bacteria, plants, and animals, but only little information is available on the synthesis of glycinebetaine in tree plants. Among four mangrove species, glycinebetaine could be detected only in Avicennia marina. Pinitol was the main osmoprotectant in the other three spec...

journal_title：Plant molecular biology

authors： Hibino T,Meng YL,Kawamitsu Y,Uehara N,Matsuda N,Tanaka Y,Ishikawa H,Baba S,Takabe T,Wada K,Ishii T,Takabe T

更新日期：2001-02-01 00:00:00

abstract：KEY MESSAGE:The design and use of existing VIGS vectors for revealing monocot gene functions are described and potential new vectors are discussed, which may expand their repertoire. Virus induced gene silencing (VIGS) is a method of transient gene silencing in plants, triggered by the use of modified viral vectors. VI...

journal_title：Plant molecular biology

authors： Kant R,Dasgupta I

更新日期：2019-05-01 00:00:00

abstract：KEY MESSAGE:An exhaustive analysis of a group of closely related parasitic plants shows a predominantly gradual reduction in plastid genome composition and provides the most reduced plastomes in the genus Cuscuta. Parasitic plants have a diminished to completely absent reliance on photosynthesis, and are characterized ...

journal_title：Plant molecular biology

authors： Banerjee A,Stefanović S

更新日期：2019-08-01 00:00:00

abstract：:We describe here the isolation and characterization of OsiSAP8, a member of stress Associated protein (SAP) gene family from rice characterized by the presence of A20 and AN1 type Zinc finger domains. OsiSAP8 is a multiple stress inducible gene, induced by various stresses, namely heat, cold, salt, desiccation, submer...

journal_title：Plant molecular biology

authors： Kanneganti V,Gupta AK

更新日期：2008-03-01 00:00:00

abstract：:StCDPK1 is a calcium dependent protein kinase expressed in tuberizing potato stolons and in sprouting tubers. StCDPK1 genomic sequence contains eight exons and seven introns, the gene structure is similar to Arabidopsis, rice and wheat CDPKs belonging to subgroup IIa. There is one copy of the gene per genome and it is...

journal_title：Plant molecular biology

authors： Gargantini PR,Giammaria V,Grandellis C,Feingold SE,Maldonado S,Ulloa RM

更新日期：2009-05-01 00:00:00