The cruciferin gene family in radish.

Abstract:

:In order to analyse the cruciferin gene family in radish a cDNA library was screened either with heterologous rapeseed probes or by differential screening and sequencing. We could identify six partial cDNA clones belonging to two different groups of cruciferin genes which do not cross-hybridize, and probably three distinct subfamilies. One of these classes corresponds to the previously described cruciferin from rapeseed and Arabidopsis. A gene corresponding to the second group, as well as its border sequences, was isolated from a radish genomic library and analysed in more detail. The cruciferin gene (cruRS) contains three introns and encodes a 479 amino acid protein. The transcription initiation site was determined. The expression of the different group of genes was studied by northern blot analysis: genes of both classes are expressed simultaneously and roughly at the same level between 25 and 35 days after flowering. Cruciferin gene copy number was estimated by Southern blot analysis. There appear to be seven or eight genes in one class and three in the other, located at different loci.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Depigny-This D,Raynal M,Aspart L,Delseny M,Grellet F

doi

10.1007/BF00040606

subject

Has Abstract

pub_date

1992-11-01 00:00:00

pages

467-79

issue

3

eissn

0167-4412

issn

1573-5028

journal_volume

20

pub_type

杂志文章
  • Specificity of auxin regulation of gibberellin 20-oxidase gene expression in pea pericarp.

    abstract::During early pea fruit growth, the physiological roles of 4-chloroindole-3-acetic acid (4-Cl-IAA) and IAA, natural pea auxins, in regulating gibberellin (GA) 20-oxidase gene expression (PsGA20ox1) were tested with 4-position, ring-substituted auxins that have a range of biological activities (fruit growth). The effect...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1015522404586

    authors: Ngo P,Ozga JA,Reinecke DM

    更新日期:2002-07-01 00:00:00

  • A novel TaMYB4 transcription factor involved in the defence response against Puccinia striiformis f. sp. tritici and abiotic stresses.

    abstract::MYB transcription factors are a large family of proteins involved in the regulation of secondary metabolism and cell shape, the enhancement of disease resistance and the response to different stresses. In this study, the role of TaMYB4 in wheat against biotic and abiotic stresses was investigated. TaMYB4 was cloned fr...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-013-0156-7

    authors: Al-Attala MN,Wang X,Abou-Attia MA,Duan X,Kang Z

    更新日期:2014-03-01 00:00:00

  • An ordered EST catalogue and gene expression profiles of cassava (Manihot esculenta) at key growth stages.

    abstract::A cDNA library was constructed from the root tissues of cassava variety Huanan 124 at the root bulking stage. A total of 9,600 cDNA clones from the library were sequenced with single-pass from the 5'-terminus to establish a catalogue of expressed sequence tags (ESTs). Assembly of the resulting EST sequences resulted i...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-010-9698-0

    authors: Li YZ,Pan YH,Sun CB,Dong HT,Luo XL,Wang ZQ,Tang JL,Chen B

    更新日期:2010-12-01 00:00:00

  • Cloning by pathway activation in yeast: identification of an Arabidopsis thaliana F-box protein that can turn on glucose repression.

    abstract::We describe a method for identifying signal transducing proteins from other organisms by their ability to turn on a signalling pathway when they are expressed at high level in yeast. The method was tested on a cDNA library from Arabidopsis thaliana, which was screened for clones that can activate glucose repression in...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1014440531842

    authors: Thelander M,Fredriksson D,Schouten J,Hoge JH,Ronne H

    更新日期:2002-05-01 00:00:00

  • Functional analysis of the grapevine paralogs of the SMG7 NMD factor using a heterolog VIGS-based gene depletion-complementation system.

    abstract::Nonsense-mediated mRNA decay (NMD) is a eukaryotic quality control system that identifies and eliminates transcripts having a premature translation termination codon (PTC). NMD is also involved in the control of several wild-type mRNAs. The NMD core machinery consists of three highly conserved NMD factors (UPF1, UPF2 ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-010-9726-0

    authors: Benkovics AH,Nyikó T,Mérai Z,Silhavy D,Bisztray GD

    更新日期:2011-02-01 00:00:00

  • Arabidopsis E2F1 binds a sequence present in the promoter of S-phase-regulated gene AtCDC6 and is a member of a multigene family with differential activities.

    abstract::Mammalian E2F transcription factors are composed of E2F and DP subunits, and with their negative regulators, Rb-related proteins, govern expression of cell-division-related genes. Six E2Fs and two DPs are present in mammals, but only single E2F genes are known from wheat, tobacco and carrot. Here we show that E2Fs are...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1011848528377

    authors: de Jager SM,Menges M,Bauer UM,Murra JA

    更新日期:2001-11-01 00:00:00

  • Phytohormone-regulated beta-amylase gene expression in rice.

    abstract::The expression of beta-amylase genes in rice (Oryza sativa) and its regulation by phytohormones gibberellic acid (GA) and abscisic acid (ABA) were examined. Upon germination beta-amylase is synthesized de novo in aleurone cells and (GA) is not required. Exogenous addition of GA does not enhance the beta-amylase activi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00040716

    authors: Wang SM,Lue WL,Eimert K,Chen J

    更新日期:1996-08-01 00:00:00

  • OsPRP3, a flower specific proline-rich protein of rice, determines extracellular matrix structure of floral organs and its overexpression confers cold-tolerance.

    abstract::Proline-rich protein (PRP), a cell wall protein of plant, has been studied in many plant species. Yet, none of the PRPs has been functionally elucidated. Here we report a novel flower-specific PRP designated OsPRP3 from rice. Expression analysis showed that the OsPRP3 transcript was mainly present in rice flower and a...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-009-9557-z

    authors: Gothandam KM,Nalini E,Karthikeyan S,Shin JS

    更新日期:2010-01-01 00:00:00

  • ZmGOLS2, a target of transcription factor ZmDREB2A, offers similar protection against abiotic stress as ZmDREB2A.

    abstract::GALACTINOL SYNTHASE is the first committed enzyme in the raffinose biosynthetic pathway. We have previously characterized the maize (Zea mays) GALACTINOL SYNTHASE2 gene (ZmGOLS2) as abiotic stress induced. To further investigate the regulation of ZmGOLS2 gene expression, individual luciferase expression vectors,in whi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-015-0403-1

    authors: Gu L,Zhang Y,Zhang M,Li T,Dirk LM,Downie B,Zhao T

    更新日期:2016-01-01 00:00:00

  • Fractionation and characterization of polyadenylated RNA from broad bean meristematic root cells.

    abstract::Several populations of polyadenylated RNA from Vicia faba méristematic root cells were fractionated by stepwise thermal elution from poly(U)-Sepharose following sequential phenol extraction. Analysis of these fractions showed that the size of the poly(A) segment could influence this fractionation, but in some cases ot...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00023013

    authors: Trapy G,Favre A,Esnault R

    更新日期:1981-03-01 00:00:00

  • Phragmoplastin dynamics: multiple forms, microtubule association and their roles in cell plate formation in plants.

    abstract::We have characterized 4 of the 16 members of the family of dynamin-related proteins (DRP) in Arabidopsis. Three members, DRP1A (previously referred as ADL1), DRP1C and DRP1E, belong to the largest group of phragmoplastin-like proteins. DRP2A (ADL6) is one of the two members that contain a pleckstrin homology (PH) doma...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/b:plan.0000006936.50532.3a

    authors: Hong Z,Geisler-Lee CJ,Zhang Z,Verma DP

    更新日期:2003-10-01 00:00:00

  • Do 14-3-3 proteins and plasma membrane H+-AtPases interact in the barley epidermis in response to the barley powdery mildew fungus?

    abstract::14-3-3 proteins form a family of highly conserved proteins with central roles in many eukaryotic signalling networks. In plants, they bind to and activate the plasma membrane H+-ATPase, creating a binding site for the phytotoxin fusicoccin. Barley 14-3-3 transcripts accumulate in the epidermis upon inoculation with th...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1014938417267

    authors: Finni C,Andersen CH,Borch J,Gjetting S,Christensen AB,de Boer AH,Thordal-Christensen H,Collinge DB

    更新日期:2002-05-01 00:00:00

  • Stress responses in alfalfa (Medicago sativa L.) 11. Molecular cloning and expression of alfalfa isoflavone reductase, a key enzyme of isoflavonoid phytoalexin biosynthesis.

    abstract::The major phytoalexin in alfalfa is the isoflavonoid (-)-medicarpin (or 6aR, 11aR)-medicarpin. Isoflavone reductase (IFR), the penultimate enzyme in medicarpin biosynthesis, is responsible for introducing one of two chiral centers in (-)-medicarpin. We have isolated a 1.18 kb alfalfa cDNA (pIFRalf1) which, when expres...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00037051

    authors: Paiva NL,Edwards R,Sun YJ,Hrazdina G,Dixon RA

    更新日期:1991-10-01 00:00:00

  • A novel chloroplast localized Rab GTPase protein CPRabA5e is involved in stress, development, thylakoid biogenesis and vesicle transport in Arabidopsis.

    abstract::A novel Rab GTPase protein in Arabidopsis thaliana, CPRabA5e (CP = chloroplast localized) is located in chloroplasts and has a role in transport. Transient expression of CPRabA5e:EGFP fusion protein in tobacco (Nicotiana tabacum) leaves, and immunoblotting using Arabidopsis showed localization of CPRabA5e in chloropla...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-013-0161-x

    authors: Karim S,Alezzawi M,Garcia-Petit C,Solymosi K,Khan NZ,Lindquist E,Dahl P,Hohmann S,Aronsson H

    更新日期:2014-04-01 00:00:00

  • Expression of lorelei-like genes in aposporous and sexual Paspalum notatum plants.

    abstract::Gametophytic apomictic plants form non-reduced embryo sacs that generate clonal embryos by parthenogenesis, in the absence of both meiosis and egg-cell fertilization. Here we report the sequence and expression analysis of a lorelei-like Paspalum notatum gene, n20gap-1, which encodes a GPI-anchored protein previously a...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-011-9814-9

    authors: Felitti SA,Seijo JG,González AM,Podio M,Laspina NV,Siena L,Ortiz JP,Pessino SC

    更新日期:2011-11-01 00:00:00

  • Nucleotide sequence of two cDNAs encoding fucoxanthin chlorophyll a/c proteins in the diatom Odontella sinensis.

    abstract::Two cDNA clones encoding fucoxanthin chlorophyll a/c-binding proteins (FCP) in the diatom Odontella sinensis have been cloned and sequenced. The derived amino acid sequences of both clones are identical, comparison of the corresponding nucleic acids reveals differences only in the third codon position, suggesting a re...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00020236

    authors: Kroth-Pancic PG

    更新日期:1995-02-01 00:00:00

  • Lysine-rich modified gamma-zeins accumulate in protein bodies of transiently transformed maize endosperms.

    abstract::During maize seed development, endosperm cells synthesize large amounts of storage proteins, alpha-, beta-, and gamma-zeins, which accumulate within endoplasmic reticulum (ER)-derived protein bodies. The absence of lysine in all zein polypeptides results in an imbalance in the amino acid composition of maize seeds. We...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1005889314967

    authors: Torrent M,Alvarez I,Geli MI,Dalcol I,Ludevid D

    更新日期:1997-05-01 00:00:00

  • Identification of cis-acting elements involved in the regulation of the pathogenesis-related gene STH-2 in potato.

    abstract::We have characterized a genomic clone containing the potato pathogenesis-related genes STH-2 and STH-21. The two genes are found 4 kb apart on the same chromosome and their sequences are highly similar. They present the same transcriptional orientation and are both interrupted by a single intron. A chimaeric gene cons...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00014935

    authors: Matton DP,Prescott G,Bertrand C,Camirand A,Brisson N

    更新日期:1993-05-01 00:00:00

  • Identification of Mutator insertional mutants of starch-branching enzyme 1 (sbe1) in Zea mays L.

    abstract::Starch-branching enzymes (SBE) alter starch structure by breaking an alpha-1,4 linkage and attaching the reducing end of the new chain to a glucan chain by an alpha 1,6 bond. In maize, three isoforms of SBE have been identified. In order to examine the function of the SBEI isoform, a reverse-genetics PCR-based screen ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1013335217744

    authors: Blauth SL,Kim KN,Klucinec J,Shannon JC,Thompson D,Guiltinan M

    更新日期:2002-02-01 00:00:00

  • Metabolic and transcriptional response of central metabolism affected by root endophytic fungus Piriformospora indica under salinity in barley.

    abstract::The root endophytic fungus Piriformospora indica enhances plant adaptation to environmental stress based on general and non-specific plant species mechanisms. In the present study, we integrated the ionomics, metabolomics, and transcriptomics data to identify the genes and metabolic regulatory networks conferring salt...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-016-0461-z

    authors: Ghaffari MR,Ghabooli M,Khatabi B,Hajirezaei MR,Schweizer P,Salekdeh GH

    更新日期:2016-04-01 00:00:00

  • The expression of Brostm, a KNOTTED1-like gene, marks the cell type and timing of in vitro shoot induction in Brassica oleracea.

    abstract::We studied the early events of de novo formation of adventitious shoot meristems in stem segments of Brassica oleracea. A regeneration system was used that is efficient, rapid, highly responsive to cytokinins and does not involve callus formation, thus allowing studies on a direct developmental switch of cells in the ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1010686931889

    authors: Teo WL,Kumar P,Goh CJ,Swarup S

    更新日期:2001-07-01 00:00:00

  • Unraveling the functions of type II-prohibitins in Arabidopsis mitochondria.

    abstract::In yeast and mammals, prohibitins (PHBs) are considered as structural proteins that form a scaffold-like structure for interacting with a set of proteins involved in various processes occurring in the mitochondria. The role of PHB in plant mitochondria is poorly understood. In the study, the model organism Arabidopsis...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-015-0320-3

    authors: Piechota J,Bereza M,Sokołowska A,Suszyński K,Lech K,Jańska H

    更新日期:2015-06-01 00:00:00

  • Mutational analysis of the conserved domains of a T-region border repeat of Agrobacterium tumefaciens.

    abstract::Left- and right-border repeats, which surround the T-region, contain two conserved regions separated by 5 bp that are not conserved. At the onset of T-DNA processing virD-encoded proteins introduce a nick in the largest of these conserved regions (12 bp) at a specific position in the bottom strand between a guanine an...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00027312

    authors: van Haaren MJ,Sedee NJ,de Boer HA,Schilperoort RA,Hooykaas PJ

    更新日期:1989-11-01 00:00:00

  • Isolation and characterization of a fruit-specific cDNA and the corresponding genomic clone from tomato.

    abstract::Differential screening of a cDNA bank constructed from ripe tomato fruit mRNA allowed the isolation of cDNA clone 2A11 which is entirely fruit-specific, is expressed at steadily increasing levels from anthesis to breaker, and accounts for approximately 1% of the messenger RNA in mature tomato fruit. A genomic clone co...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00016019

    authors: Pear JR,Ridge N,Rasmussen R,Rose RE,Houck CM

    更新日期:1989-12-01 00:00:00

  • Gene expression during anthesis and senescence in Iris flowers.

    abstract::We investigated changes in gene expression in Iris hollandica flowers by microarray technology. Flag tepals were sampled daily, from three days prior to flower opening to the onset of visible senescence symptoms. Gene expression profiles were compared with biochemical data including lipid and protein degradation and D...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/B:PLAN.0000023670.61059.1d

    authors: van Doorn WG,Balk PA,van Houwelingen AM,Hoeberichts FA,Hall RD,Vorst O,van der Schoot C,van Wordragen MF

    更新日期:2003-12-01 00:00:00

  • Stress responses in alfalfa (Medicago sativa L.) XIX. Transcriptional activation of oxidative pentose phosphate pathway genes at the onset of the isoflavonoid phytoalexin response.

    abstract::We have isolated cDNA clones encoding the pentose phosphate pathway enzymes 6-phosphogluconate dehydrogenase (6PGDH, EC 1.1.1.44) and glucose 6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) from alfalfa (Medicago sativa L.). These exhibit extensive nucleotide and amino acid sequence similarity to the corresponding gene...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00042073

    authors: Fahrendorf T,Ni W,Shorrosh BS,Dixon RA

    更新日期:1995-08-01 00:00:00

  • Zrp2: a novel maize gene whose mRNA accumulates in the root cortex and mature stems.

    abstract::A near full-length cDNA clone (pZRP2) was isolated from a cDNA library constructed from maize root mRNAs. The predicted polypeptide has a calculated molecular mass of 66,975 Da, is largely hydrophilic, and contains 26 repeats of a motif the consensus sequence of which is RKATTSYG[S][D/E][D/E][D/E][D/E][P]. The functio...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1005830313272

    authors: Held BM,John I,Wang H,Moragoda L,Tirimanne TS,Wurtele ES,Colbert JT

    更新日期:1997-10-01 00:00:00

  • Molecular characterization of rice hsp101: complementation of yeast hsp104 mutation by disaggregation of protein granules and differential expression in indica and japonica rice types.

    abstract::HSP100 protein is an important component of the heat-shock response in diverse organisms. Using specific primers based on cDNA sequence, rice hsp101 gene was PCR-amplified and sequenced. Southern analysis revealed that there appears to be a single gene per haploid genome coding for HSP101 protein in rice. Northern ana...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1022324920316

    authors: Agarwal M,Sahi C,Katiyar-Agarwal S,Agarwal S,Young T,Gallie DR,Sharma VM,Ganesan K,Grover A

    更新日期:2003-03-01 00:00:00

  • Chloroplast and cytosolic triosephosphate isomerases from spinach: purification, microsequencing and cDNA cloning of the chloroplast enzyme.

    abstract::Chloroplast and cytosolic triosephosphate isomerases from spinach were separated and purified to homogeneity. Both enzymes were partially sequenced by Edman degradation. Using degenerate primers designed against the amino acid sequences, a homologous probe for the chloroplast enzyme was amplified and used to isolate s...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00019506

    authors: Henze K,Schnarrenberger C,Kellermann J,Martin W

    更新日期:1994-12-01 00:00:00

  • A role of OsGA20ox1 , encoding an isoform of gibberellin 20-oxidase, for regulation of plant stature in rice.

    abstract::Gibberellin (GA) 20-oxidase (GA20ox) is a key enzyme that normally catalyzes the penultimate steps in GA biosynthesis. One of the GA20ox genes in rice (Oryza sativa L.), OsGA20ox2 ( SD1 ), is well known as the "Green Revolution gene", and loss-of function mutation in this locus causes semi-dwarfism. Another GA20ox gen...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-004-1692-y

    authors: Oikawa T,Koshioka M,Kojima K,Yoshida H,Kawata M

    更新日期:2004-07-01 00:00:00