当前位置： SCI文献检索 > PLANT MOLECULAR BIOLOGY期刊下所有文献 > Identification of Mutator insertional mutants of starch-branching enzyme 1 (sbe1) in Zea mays L.

Identification of Mutator insertional mutants of starch-branching enzyme 1 (sbe1) in Zea mays L.

Abstract：

:Starch-branching enzymes (SBE) alter starch structure by breaking an alpha-1,4 linkage and attaching the reducing end of the new chain to a glucan chain by an alpha 1,6 bond. In maize, three isoforms of SBE have been identified. In order to examine the function of the SBEI isoform, a reverse-genetics PCR-based screen was used to identify a mutant line segregating for a Mutator transposon within Sbe1. Compared to wild-type controls, Sbe1 transcripts accumulate at extremely low levels in leaves of the homozygous mutant. Antibodies failed to detect SBEI in leaf tissue of mutants or wild-type controls. In contrast, the level of SBEI in endosperm is undetectable in homozygous mutants while easily detected in wild-type controls. Starches extracted from mutant leaves and endosperm have structures indistinguishable from starches of wild-type controls as determined by size-exclusion chromatography (SEC) of intact starch and high-performance SEC of debranched starch. To investigate the possibility of compensation for the lack of SBEI by expression of the homologous sequence reported by Kim etal. (1998), agenomic fragment (Sbe1b) of this sequence was cloned. Northern hybridizations of mutant leaf, root, tassel, endosperm and embryo tissues with non-specific Sbelb probes failed to reveal expression of the homologous sequence. These results suggest that the homologous sequence is not compensating for a lack of SBEI in sbe1::Mu mutants. Further study of this sbel mutation in the presence of other genetic mutations may help to understand the role of SBEI in determining starch structure in leaves and endosperm.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Blauth SL,Kim KN,Klucinec J,Shannon JC,Thompson D,Guiltinan M

doi

10.1023/a:1013335217744

subject

Has Abstract

pub_date

2002-02-01 00:00:00

pages

287-97

issue

3

eissn

0167-4412

issn

1573-5028

journal_volume

48

pub_type

杂志文章

相关文献

PLANT MOLECULAR BIOLOGY文献大全
  • The highly expressed tapetum-specific A9 gene is not required for male fertility in Brassica napus.

    abstract：:An antisense approach was used to attempt to determine the function of the highly abundant, tapetum-specific A9 transcript in microsporogenesis. A Brassica napus A9 cDNA clone was linked in sense and antisense orientations to the Arabidopsis thaliana A9 promoter and the resulting chimaeric genes introduced into B. nap...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00040577

    authors： Turgut K,Barsby T,Craze M,Freeman J,Hodge R,Paul W,Scott R

    更新日期：1994-01-01 00:00:00

  • CYP71Z18 overexpression confers elevated blast resistance in transgenic rice.

    abstract：KEY MESSAGE:CYP71Z18 exhibited plastic substrate specificity to catalyze oxidation of multiple rice diterpenes and elevated chemical defense against the blast fungus in transgenic rice. Diversified plant specialized metabolism relies on corresponding biosynthetic enzymes with differential substrate specificity. CYP71Z1...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-019-00881-3

    authors： Shen Q,Pu Q,Liang J,Mao H,Liu J,Wang Q

    更新日期：2019-08-01 00:00:00

  • cDNA cloning of two isoforms of ornithine carbamoyltransferase from Canavalia lineata leaves and the effect of site-directed mutagenesis of the carbamoyl phosphate binding site.

    abstract：:The immunoscreening method was used to isolate cDNAs of 1323 bp (ClOCT1) and 1433 bp (ClOCT2) encoding two ornithine carbamoyltransferases (OCT, EC 2.1.3.3) from the cDNA expression library of Canavalia lineata leaves constructed in a lambdaZAP Express vector. ClOCT1 and ClOCT2 encode 359 and 369 amino acids, respecti...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1023/a:1011632927541

    authors： Lee Y,Choi YA,Hwang ID,Kim SG,Kwon YM

    更新日期：2001-08-01 00:00:00

  • Identification of cis-regulatory elements required for endosperm expression of the rice storage protein glutelin gene GluB-1.

    abstract：:Rice storage protein glutelin genes are coordinately regulated during seed development. A previous 5' deletion analysis using transgenic tobacco revealed that the minimum 5' region necessary for endosperm specificity was within -245 bp of the transcription start site, and included the AACA and GCN4 motifs that are hig...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1023/a:1026459229671

    authors： Washida H,Wu CY,Suzuki A,Yamanouchi U,Akihama T,Harada K,Takaiwa F

    更新日期：1999-05-01 00:00:00

  • Expression of the Pib rice-blast-resistance gene family is up-regulated by environmental conditions favouring infection and by chemical signals that trigger secondary plant defences.

    abstract：:The rice blast resistance gene Pib is a member of the nucleotide binding site (NBS) and leucine-rich repeat (LRR) class of plant disease resistance (R) genes and belongs to a small gene family. We describe here the isolation and characterization of a Pib homologue (PibH8), and extensive investigation of the expression...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1023/a:1012457113700

    authors： Wang ZX,Yamanouchi U,Katayose Y,Sasaki T,Yano M

    更新日期：2001-11-01 00:00:00

  • An ordered EST catalogue and gene expression profiles of cassava (Manihot esculenta) at key growth stages.

    abstract：:A cDNA library was constructed from the root tissues of cassava variety Huanan 124 at the root bulking stage. A total of 9,600 cDNA clones from the library were sequenced with single-pass from the 5'-terminus to establish a catalogue of expressed sequence tags (ESTs). Assembly of the resulting EST sequences resulted i...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-010-9698-0

    authors： Li YZ,Pan YH,Sun CB,Dong HT,Luo XL,Wang ZQ,Tang JL,Chen B

    更新日期：2010-12-01 00:00:00

  • Characterization of DRGs, developmentally regulated GTP-binding proteins, from pea and Arabidopsis.

    abstract：:Developmentally regulated GTP-binding proteins (DRGs) from animals and fungi are highly conserved but have no known function. Here we characterize DRGs from pea (PsDRG) and Arabidopsis (AtDRG). Amino acid sequences of AtDRG and PsDRG were 90% identical to each other and about 65% identical to human DRG. Genomic Southe...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1023/a:1006178710443

    authors： Devitt ML,Maas KJ,Stafstrom JP

    更新日期：1999-01-01 00:00:00

  • Calmodulin-like proteins from Arabidopsis and tomato are involved in host defense against Pseudomonas syringae pv. tomato.

    abstract：:Complex signal transduction pathways underlie the myriad plant responses to attack by pathogens. Ca(2+) is a universal second messenger in eukaryotes that modulates various signal transduction pathways through stimulus-specific changes in its intracellular concentration. Ca(2+)-binding proteins such as calmodulin (CaM...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-005-8395-x

    authors： Chiasson D,Ekengren SK,Martin GB,Dobney SL,Snedden WA

    更新日期：2005-08-01 00:00:00

  • A Brassica napus PHT1 phosphate transporter, BnPht1;4, promotes phosphate uptake and affects roots architecture of transgenic Arabidopsis.

    abstract：:Phosphorus (P) is one of the essential nutrient elements for plant development. In this work, BnPht1;4 gene, encoding a phosphate transporter of PHT1 family, was isolated from Brassica napus. BnPht1;4 possesses the major characteristic of PHT1 high-affinity Pi transporters in plants, such as plasma-membrane localizati...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-014-0249-y

    authors： Ren F,Zhao CZ,Liu CS,Huang KL,Guo QQ,Chang LL,Xiong H,Li XB

    更新日期：2014-12-01 00:00:00

  • OsYSL18 is a rice iron(III)-deoxymugineic acid transporter specifically expressed in reproductive organs and phloem of lamina joints.

    abstract：:Iron uptake and translocation in plants are important processes for both plant and human nutrition, whereas relatively little is known about the molecular mechanisms of iron transport within the plant body. Several reports have shown that yellow stripe 1 (YS1) and YS1-like (YSL) transporters mediate metal-phytosiderop...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-009-9500-3

    authors： Aoyama T,Kobayashi T,Takahashi M,Nagasaka S,Usuda K,Kakei Y,Ishimaru Y,Nakanishi H,Mori S,Nishizawa NK

    更新日期：2009-08-01 00:00:00

  • TGA3 is a distinct member of the TGA family of bZIP transcription factors in Arabidopsis thaliana.

    abstract：:TGA1a is a well-characterized transcription factor that may mediate the root-specific and auxin-responsive expression of some plant genes. In tobacco, Southern blot and genomic cloning analyses have shown that TGA1a consists of at least four closely related genes. Since TGA1a belongs to the bZIP class of transcription...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00024193

    authors： Miao ZH,Liu X,Lam E

    更新日期：1994-04-01 00:00:00

  • Ectopic expression of the Arabidopsis MINI ZINC FINGER1 and MIF3 genes induces shoot meristems on leaf margins.

    abstract：:A leaf undergoes determinate growth from a primordium on flank of the shoot apical meristem. Several intrinsic pathways restrict meristematic activity in the leaf of Arabidopsis; however, other factors remain to be defined. We report here that the overexpression of MINI ZINC FINGER1 (MIF1) or MIF3 disrupted the leaf d...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-011-9768-y

    authors： Hu W,Feng B,Ma H

    更新日期：2011-05-01 00:00:00

  • EGY1 plays a role in regulation of endodermal plastid size and number that are involved in ethylene-dependent gravitropism of light-grown Arabidopsis hypocotyls.

    abstract：:Egy1 was isolated as an ethylene-dependent gravitropism-deficient Arabidopsis mutant. Molecular studies reveal that EGY1 gene encodes a 59-kDa plastid-targeted metalloprotease. It is actively expressed in hypocotyl tissue and targets to endodermal and cortex plastid. Its protein level is up-regulated by both ethylene ...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-007-9273-5

    authors： Guo D,Gao X,Li H,Zhang T,Chen G,Huang P,An L,Li N

    更新日期：2008-03-01 00:00:00

  • Ethylene regulates the expression of a cysteine proteinase gene during germination of chickpea (Cicer arietinum L.).

    abstract：:Synthetic oligonucleotides corresponding to conserved regions of cysteine proteinases were used as primers in the RT-PCR amplification of a fragment of cDNA corresponding to a region of a cysteine proteinase gene expressed during germination of chickpea (cac for Cicer arietinum cysteine proteinase). The identity of th...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00023238

    authors： Cervantes E,Rodríguez A,Nicolás G

    更新日期：1994-05-01 00:00:00

  • Sequence-guided approach to genotyping plant clones and species using polymorphic NB-ARC-related genes.

    abstract：KEY MESSAGE:Leveraging the heightened levels of polymorphism in NB-ARC-related protein encoding genes in higher plants, a bioinformatic pipeline was created to identify regions in this gene family from sequenced plant genomes that exhibit fragment length or single nucleotide differences in different accessions of the s...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-018-0774-1

    authors： Chu P,Wilson GM,Michael TP,Vaiciunas J,Honig J,Lam E

    更新日期：2018-10-01 00:00:00

  • The Arabidopsis homeodomain-leucine zipper II gene family: diversity and redundancy.

    abstract：:The Arabidopsis genome contains 10 genes belonging to the HD-Zip II family including ATHB2 and HAT2. Previous work has shown that ATHB2 is rapidly and strongly induced by light quality changes that provoke the shade avoidance response whereas HAT2 expression responds to auxin. Here, we present a genome-wide analysis o...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-008-9383-8

    authors： Ciarbelli AR,Ciolfi A,Salvucci S,Ruzza V,Possenti M,Carabelli M,Fruscalzo A,Sessa G,Morelli G,Ruberti I

    更新日期：2008-11-01 00:00:00

  • Regulation of transcription by the Arabidopsis UVR8 photoreceptor involves a specific histone modification.

    abstract：:The photoreceptor UV RESISTANCE LOCUS 8 (UVR8) specifically mediates photomorphogenic responses to UV-B wavelengths. UVR8 acts by regulating transcription of a set of genes, but the underlying mechanisms are unknown. Previous research indicated that UVR8 can associate with chromatin, but the specificity and functional...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-016-0522-3

    authors： Velanis CN,Herzyk P,Jenkins GI

    更新日期：2016-11-01 00:00:00

  • The protein-encoding gene T-urf13 is not edited in maize mitochondria.

    abstract：:RNA editing of T-urf13, a gene specific to the mitochondria of cytoplasmic male-sterile, type-T (cms-T) maize, and an adjacent, cotranscribed gene orf221, have been studied by cDNA sequencing. No editing was detected in 22 cDNA clones. This is the only report of a polypeptide-encoding gene in higher-plant mitochondria...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00037148

    authors： Ward GC,Levings CS 3rd

    更新日期：1991-11-01 00:00:00

  • The Arabidopsis thaliana GSK3/Shaggy like kinase AtSK3-2 modulates floral cell expansion.

    abstract：:The GSK3/Shaggy family of serine/threonine protein kinases is involved in a series of biological processes in animals, plants and yeast [Charrier et al. (2002) Plant Physiol 130:577-590; Jope and Johnson (2004) Trends Biochem Sci 29:95-102; Li and Nam (2002) Science 295:1299-1301; Piao et al. (2001) Plant J 27:305-314...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-007-9138-y

    authors： Claisse G,Charrier B,Kreis M

    更新日期：2007-05-01 00:00:00

  • A maize histone deacetylase and retinoblastoma-related protein physically interact and cooperate in repressing gene transcription.

    abstract：:In mammalian cells the product of the human retinoblastoma tumour suppressor gene (pRb) can recruit Rpd3-like histone deacetylases to repress transcription. In this study, we investigated whether this mechanism might also be relevant in plants and found both conserved and distinct features. The expression profiles of ...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1023/a:1022090916446

    authors： Rossi V,Locatelli S,Lanzanova C,Boniotti MB,Varotto S,Pipal A,Goralik-Schramel M,Lusser A,Gatz C,Gutierrez C,Motto M

    更新日期：2003-02-01 00:00:00

  • The reconstruction and biochemical characterization of ancestral genes furnish insights into the evolution of terpene synthase function in the Poaceae.

    abstract：KEY MESSAGE:Distinct catalytic features of the Poaceae TPS-a subfamily arose early in grass evolution and the reactions catalyzed have become more complex with time. The structural diversity of terpenes found in nature is mainly determined by terpene synthases (TPS). TPS enzymes accept ubiquitous prenyl diphosphates as...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-020-01037-4

    authors： Luck K,Chen X,Norris AM,Chen F,Gershenzon J,Köllner TG

    更新日期：2020-09-01 00:00:00

  • Quantitative variation in components of the maize mitochondrial genome between tissues and between plants with different male-sterile cytoplasms.

    abstract：:The amounts of a 1.9 kb mitochondrial plasmid relative to sequences in another mitochondrial DNA replicon and also to nuclear ribosomal DNA sequences have been compared in maize leaves and anthers. Similar comparisons have been made between plants with the same nuclear genotype but containing normal, S, or T cytoplasm...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF02418241

    authors： Abbott AG,O'Dell M,Flavell RB

    更新日期：1985-07-01 00:00:00

  • Isolation of rapeseed genes expressed early and specifically during development of the male gametophyte.

    abstract：:A cDNA subtraction and differential hybridization strategy was used to isolate cDNAs expressed early during male gametophyte development in the important crop species Brassica napus. Three cDNAs, corresponding to genes highly and specifically expressed at the tetrad and microspore stages, are presented here. The analy...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1023/a:1006282507095

    authors： Fourgoux-Nicol A,Drouaud J,Haouazine N,Pelletier G,Guerche P

    更新日期：1999-07-01 00:00:00

  • Zrp2: a novel maize gene whose mRNA accumulates in the root cortex and mature stems.

    abstract：:A near full-length cDNA clone (pZRP2) was isolated from a cDNA library constructed from maize root mRNAs. The predicted polypeptide has a calculated molecular mass of 66,975 Da, is largely hydrophilic, and contains 26 repeats of a motif the consensus sequence of which is RKATTSYG[S][D/E][D/E][D/E][D/E][P]. The functio...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1023/a:1005830313272

    authors： Held BM,John I,Wang H,Moragoda L,Tirimanne TS,Wurtele ES,Colbert JT

    更新日期：1997-10-01 00:00:00

  • The small auxin-up RNA OsSAUR45 affects auxin synthesis and transport in rice.

    abstract：KEY MESSAGE:This research is the first to demonstrate that OsSAUR45 is involved in plant growth though affecting auxin synthesis and transport by repressing OsYUCCA and OsPIN gene expression in rice. Small auxin-up RNAs (SAURs) comprise a large multigene family and are rapidly activated as part of the primary auxin res...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-017-0595-7

    authors： Xu YX,Xiao MZ,Liu Y,Fu JL,He Y,Jiang DA

    更新日期：2017-05-01 00:00:00

  • Cloning and analysis of the gene for cystathionine gamma-synthase from Arabidopsis thaliana.

    abstract：:A cDNA clone, CGS1, encoding cystathionine gamma-synthase (CGS) from Arabidopsis thaliana was selected by complementation of CGS mutant strain of Escherichia coli (metB). Cells expressing CGS1 can grow on medium lacking Met and contain CGS enzyme activity. Genomic DNA blot analysis of A. thaliana revealed that there i...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00041395

    authors： Kim J,Leustek T

    更新日期：1996-12-01 00:00:00

  • Functional characterization of four APETALA2-family genes (RAP2.6, RAP2.6L, DREB19 and DREB26) in Arabidopsis.

    abstract：:APETALA2 (AP2) transcription factors (TFs) play very important roles in plant growth and development and in defense response. Here, we report functional characterization of four AP2 TF family genes [(RAP2.6 (At1g43160), RAP2.6L (At5g13330), DREB 26 (At1g21910) and DREB19 (At2g38340)] that were identified among NaCl in...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-010-9711-7

    authors： Krishnaswamy S,Verma S,Rahman MH,Kav NN

    更新日期：2011-01-01 00:00:00

  • The transcriptome of NaCl-treated Limonium bicolor leaves reveals the genes controlling salt secretion of salt gland.

    abstract：:Limonium bicolor, a typical recretohalophyte that lives in saline environments, excretes excessive salt to the environment through epidermal salt glands to avoid salt stress. The aim of this study was to screen for L. bicolor genes involved in salt secretion by high-throughput RNA sequencing. We established the experi...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-016-0460-0

    authors： Yuan F,Lyu MJ,Leng BY,Zhu XG,Wang BS

    更新日期：2016-06-01 00:00:00

  • Differential expression of TPS11, a phosphate starvation-induced gene in tomato.

    abstract：:Plants respond to phosphate (Pi) deficiency through adaptive mechanisms involving several morphological, biochemical and molecular changes. In this study, we have characterized the structure and expression of a tomato (Lycopersicon esculentum L.) phosphate starvation-induced gene (TPSI1). A 3.5 kb genomic fragment con...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1023/a:1005729309569

    authors： Liu C,Muchhal US,Raghothama KG

    更新日期：1997-03-01 00:00:00

  • The molecular and biochemical basis for varietal variation in sesquiterpene content in melon (Cucumis melo L.) rinds.

    abstract：:A combined chemical, biochemical and molecular study was conducted to understand the differential accumulation of volatile sesquiterpenes in melon fruits. Sesquiterpenes were present mainly in the rinds of climacteric varieties, and a great diversity in their composition was found among varieties. Sesquiterpenes were ...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-008-9296-6

    authors： Portnoy V,Benyamini Y,Bar E,Harel-Beja R,Gepstein S,Giovannoni JJ,Schaffer AA,Burger J,Tadmor Y,Lewinsohn E,Katzir N

    更新日期：2008-04-01 00:00:00