Mutational analysis of the conserved domains of a T-region border repeat of Agrobacterium tumefaciens.

abstract：:The expression of the mat-r locus (mat-r-nad1e-nad5III) was studied in wheat mitochondria. Transcription initiation sites were mapped by S1 protection, primer extension and capping experiments. Two different transcription initiation sites were found. One, non-canonical promoter of low expression level generates a tran...

journal_title：Plant molecular biology

authors： Farré JC,Araya A

更新日期：1999-08-01 00:00:00

abstract：:We have constructed a chimaeric gene consisting of the promoter of the soybean heat shock (hs) gene Gmhsp17, 6-L, the coding region of a hygromycin phosphotransferase (hpt) gene, and the termination sequence of the nopaline synthase (nos) gene. This gene fusion was introduced into tobacco by Agrobacterium-mediated gen...

journal_title：Plant molecular biology

authors： Severin K,Schöffl F

更新日期：1990-12-01 00:00:00

abstract：:The nucleotide sequence for the Photosystem II gene psbC has been determined for the cyanobacterium Synechocystis 6803. The gene overlaps the last 50 bases of the psbD gene, and both genes are transcribed in the same direction, but read in different frames. This arrangement is identical to that found in all chloroplas...

journal_title：Plant molecular biology

authors： Chisholm D,Williams JG

更新日期：1988-07-01 00:00:00

abstract：:Retroviruses and retrotransposons make up the broad class of retroelements replicating and transposing via reverse transcriptase. Retroelements have recently been found to be ubiquitous in the plants. We report here the isolation, sequence and analysis of a retroelement from barley (Hordeum vulgare L.) with all the fe...

journal_title：Plant molecular biology

authors： Manninen I,Schulman AH

更新日期：1993-08-01 00:00:00

abstract：:The High Mobility Group (HMG) proteins of vertebrate animals have been the subject of intensive study because of evidence that they may be structural proteins of transcriptionally active chromatin. Organisms other than vertebrate animals have chromatin proteins which meet the operational criteria of salt extractabilit...

journal_title：Plant molecular biology

authors： Spiker S,Everett KM

更新日期：1987-09-01 00:00:00

abstract：:Gibberellin (GA) 20-oxidase (GA20ox) is a key enzyme that normally catalyzes the penultimate steps in GA biosynthesis. One of the GA20ox genes in rice (Oryza sativa L.), OsGA20ox2 ( SD1 ), is well known as the "Green Revolution gene", and loss-of function mutation in this locus causes semi-dwarfism. Another GA20ox gen...

journal_title：Plant molecular biology

authors： Oikawa T,Koshioka M,Kojima K,Yoshida H,Kawata M

更新日期：2004-07-01 00:00:00

abstract：:As part of a strategy to define differences in genome organization and expression between cytoplasmic male-sterile (CMS) and male-fertile (MF) sugar-beet mitochondria, cDNA libraries from both mitochondrial genotypes were constructed. Preliminary screening with ribosomal RNA gene probes identified candidate cDNA clone...

journal_title：Plant molecular biology

authors： Xue Y,Collin S,Davies DR,Thomas CM

更新日期：1994-04-01 00:00:00

abstract：:Damage to DNA induced by ultraviolet light can be reversed by a blue light-dependent reaction catalyzed by enzymes called DNA photolyases. Chlamydomonas has been shown to have DNA photolyase activity in both the nucleus and the chloroplast. Here we report the cloning and sequencing of a gene, PHR2, from Chlamydomonas ...

journal_title：Plant molecular biology

authors： Petersen JL,Lang DW,Small GD

更新日期：1999-08-01 00:00:00

abstract：:Tomato golden mosaic virus (TGMV), a member of the geminivirus group, has a genome consisting of two DNA molecules designated the A and B components. Both are required for infectivity in healthy plants, although the former has been shown to replicate independently in transgenic plants containing tandem direct repeats ...

journal_title：Plant molecular biology

authors： Sunter G,Gardiner WE,Rushing AE,Rogers SG,Bisaro DM

更新日期：1987-11-01 00:00:00

abstract：:An antisense approach was used to attempt to determine the function of the highly abundant, tapetum-specific A9 transcript in microsporogenesis. A Brassica napus A9 cDNA clone was linked in sense and antisense orientations to the Arabidopsis thaliana A9 promoter and the resulting chimaeric genes introduced into B. nap...

journal_title：Plant molecular biology

authors： Turgut K,Barsby T,Craze M,Freeman J,Hodge R,Paul W,Scott R

更新日期：1994-01-01 00:00:00

abstract：:We have identified 39 Arabidopsis thaliana ESTs encoding germin-like proteins (GLPs) and have completely sequenced 25 of these cDNAs. Our analysis demonstrates that the Arabidopsis genome contains a gene family with at least 12 GLP genes. Comparisons with other known germins and germin-like proteins indicate that thes...

journal_title：Plant molecular biology

authors： Carter C,Graham RA,Thornburg RW

更新日期：1998-12-01 00:00:00

abstract：:Drought is one of the critical factors limiting reproductive yields of rice and other crops globally. However, little is known about the molecular mechanism underlying reproductive development under drought stress in rice. To explore the potential gene function for improving rice reproductive development under drought...

journal_title：Plant molecular biology

authors： Guo C,Ge X,Ma H

更新日期：2013-06-01 00:00:00

abstract：:The effect of red light and far-red light on the appearance of mRNA for the small and large subunit of ribulose-1,5-bisphosphate carboxylase in rye seedlings has been analysed. Irradiation of etiolated seedlings with a pulse of red light increased the mRNA level of the small subunit by a factor of 7-10 and that of the...

journal_title：Plant molecular biology

authors： Ernst D,Pfeiffer F,Schefbeck K,Weyrauch C,Oesterhelt D

更新日期：1987-01-01 00:00:00

abstract：:We used a functional screening method to isolate genes whose products elicit the hypersensitive response (HR) pathway of defense against plant pathogens. A cDNA library derived from tobacco leaves undergoing the HR was cloned into a tobacco mosaic virus (TMV)-based expression vector. Infectious transcripts were genera...

journal_title：Plant molecular biology

authors： Karrer EE,Beachy RN,Holt CA

更新日期：1998-03-01 00:00:00

abstract：:In yeast, Rad21/Scc1 and its meiotic variant Rec8 are key players in the establishment and subsequent dissolution of sister chromatid cohesion for mitosis and meiosis, respectively, which are essential for chromosome segregation. Unlike yeast, our identification revealed that the rice genome has 4 RAD21-like genes tha...

journal_title：Plant molecular biology

authors： Zhang L,Tao J,Wang S,Chong K,Wang T

更新日期：2006-03-01 00:00:00

abstract：:We investigated the effects of expressing a cDNA of cdc25, a mitotic inducer gene of Schizosaccharomyces pombe, on the development of transgenic tobacco plants (Nicotiana tabacum cv. Samsun). Nine independent primary transformants were regenerated containing the cdc25 sequence under the control of a cauliflower mosaic...

journal_title：Plant molecular biology

authors： Bell MH,Halford NG,Ormrod JC,Francis D

更新日期：1993-11-01 00:00:00

abstract：:During early pea fruit growth, the physiological roles of 4-chloroindole-3-acetic acid (4-Cl-IAA) and IAA, natural pea auxins, in regulating gibberellin (GA) 20-oxidase gene expression (PsGA20ox1) were tested with 4-position, ring-substituted auxins that have a range of biological activities (fruit growth). The effect...

journal_title：Plant molecular biology

authors： Ngo P,Ozga JA,Reinecke DM

更新日期：2002-07-01 00:00:00

abstract：:Plant microRNAs (miRNAs) act as negative regulators of gene expression by slicing target transcripts or inhibiting translation. A number of miRNAs play important roles in development. In order to investigate the potential function of miRNAs during male gametogenesis in rice, we obtained both gene and small RNA express...

journal_title：Plant molecular biology

authors： Peng H,Chun J,Ai TB,Tong YA,Zhang R,Zhao MM,Chen F,Wang SH

更新日期：2012-09-01 00:00:00

abstract：:A novel rice genomic sequence encoding coding segments homologous to other metallothionein-like genes was isolated from Oryza sativa genomic library. This sequence, hereby designated as rgMT (rice genomic metallothionein-like gene), consists of two exons and one intron. From the coding sequence, it is predicted that r...

journal_title：Plant molecular biology

authors： Hsieh HM,Liu WK,Huang PC

更新日期：1995-06-01 00:00:00

abstract：KEY MESSAGE:Plant bioregulators play an important role in managing oxidative stress tolerance in plants. Utilizing their ability in stress sensitive crops through genetic engineering will be a meaningful approach to manage food production under the threat of climate change. Exploitation of the plant defense system agai...

journal_title：Plant molecular biology

authors： Zulfiqar F,Ashraf M

更新日期：2021-01-01 00:00:00

abstract：:In this paper we report on the organization and expression of the two chalcone flavanone isomerase (CHI) genes A and B from thePetunia hybrida inbred line V30. From a combination of sequence data, primer extension and RNAse protection experiments we infer the presence of two promoters PA1 and PA2 upstream of the CHI g...

journal_title：Plant molecular biology

authors： van Tunen AJ,Hartman SA,Mur LA,Mol JN

更新日期：1989-05-01 00:00:00

abstract：:We have characterized the topoisomerase I and II activities in nuclear extracts from immature embryos of Zea mays and the effect of the treatment with 2,4-dichlorophenoxyacetic acid (2,4-D) and abscisic acid (ABA). These extracts were shown to be essentially devoid of protease and nuclease activities and they were tes...

journal_title：Plant molecular biology

authors： Carballo M,Giné R,Santos M,Puigdomènech P

更新日期：1991-01-01 00:00:00

abstract：:Chromosomal non-histone high-mobility-group (HMG) proteins represent essential components of eukaryotic chromatin and have also been isolated from a variety of plants. In maize, studies on structure and function of the two larger of the four major HMG proteins have recently been performed and are now extended by analy...

journal_title：Plant molecular biology

authors： Grasser KD,Hetz W,Feix G

更新日期：1994-06-01 00:00:00

abstract：:Seed dormancy is an important developmental process that prevents pre-harvest sprouting in many grains and other seeds. Abscisic acid (ABA), a plant hormone, plays a crucial role in regulating dormancy but the underlying molecular regulatory mechanisms are not fully understood. An Arabidopsis zinc-finger gene, MEDIATO...

journal_title：Plant molecular biology

authors： He Y,Gan S

更新日期：2004-01-01 00:00:00

abstract：:Cab-1 is a complex genetic locus in tomato consisting of four clustered genes encoding chlorophyll a/b-binding polypeptide. Southern blot analysis of total tomato DNA with genomic clones corresponding to the Cab-1 locus has revealed the presence of a repetitive element in the 3 kb spacer regions between two of these g...

journal_title：Plant molecular biology

authors： Bernatzky R,Pichersky E,Malik VS,Tanksley SD

更新日期：1988-09-01 00:00:00

abstract：:In yeast and mammals, prohibitins (PHBs) are considered as structural proteins that form a scaffold-like structure for interacting with a set of proteins involved in various processes occurring in the mitochondria. The role of PHB in plant mitochondria is poorly understood. In the study, the model organism Arabidopsis...

journal_title：Plant molecular biology

authors： Piechota J,Bereza M,Sokołowska A,Suszyński K,Lech K,Jańska H

更新日期：2015-06-01 00:00:00

abstract：:Developmentally regulated GTP-binding proteins (DRGs) from animals and fungi are highly conserved but have no known function. Here we characterize DRGs from pea (PsDRG) and Arabidopsis (AtDRG). Amino acid sequences of AtDRG and PsDRG were 90% identical to each other and about 65% identical to human DRG. Genomic Southe...

journal_title：Plant molecular biology

authors： Devitt ML,Maas KJ,Stafstrom JP

更新日期：1999-01-01 00:00:00

abstract：:The complex capitulum of Chrysanthemum morifolium is often comprised of bilaterally symmetrical ray florets and radially symmetrical disc florets. The TCP transcription factor clade CYCLOIDEA2 (CYC2) appears to play a vital role in determining floral symmetry and in regulating floral organ development in Asteraceae. O...

journal_title：Plant molecular biology

authors： Yuan C,Huang D,Yang Y,Sun M,Cheng T,Wang J,Pan H,Zhang Q

更新日期：2020-05-01 00:00:00

abstract：:An Arabidopsis thaliana gene (UBC6) encoding a homologue to ubiquitin-conjugating enzymes has been isolated which is capable of encoding a protein of 183 amino acids of ca. 21 kDa. Northern analysis indicates that the gene is expressed in flowers, seeds and, to a somewhat lesser extent, in 10-day seedlings but not in ...

journal_title：Plant molecular biology

authors： Watts FZ,Butt N,Layfield P,Machuka J,Burke JF,Moore AL

更新日期：1994-10-01 00:00:00

abstract：:cDNA clones for all enzymes of the prechorismate pathway of higher plants have previously been cloned, with the exception of the second enzyme of the pathway, 3-dehydroquinate synthase. Here we describe the isolation of a cDNA encoding a 3-dehydroquinate synthase from tomato which was identified by complementing a 3-d...

journal_title：Plant molecular biology

authors： Bischoff M,Rösler J,Raesecke HR,Görlach J,Amrhein N,Schmid J

更新日期：1996-04-01 00:00:00