A novel zinc-finger protein with a proline-rich domain mediates ABA-regulated seed dormancy in Arabidopsis.

abstract：:We have cloned and sequenced a full-length cDNA for uroporphyrinogen decarboxylase (UROD, EC 4.1.1.37) from tobacco (Nicotiana tabacum L.) and a partial cDNA clone from barley (Hordeum vulgare L.). The cDNA of tobacco encodes a protein of 43 kDa, which has 33% overall similarity to UROD sequences determined from other...

journal_title：Plant molecular biology

authors： Mock HP,Trainotti L,Kruse E,Grimm B

更新日期：1995-05-01 00:00:00

abstract：:Accumulation and stability of psbA and rbcL-S transcripts in Synechocystis 6803 was followed in the presence and absence of the photosynthesis inhibitors DCMU and methylviologen. Our results demonstrate that both transcript production and transcript stability are important regulatory elements for psbA gene expression ...

journal_title：Plant molecular biology

authors： Mohamed A,Jansson C

更新日期：1991-05-01 00:00:00

abstract：:Gibberellins (GAs) are important plant growth regulators, regulating many plant developmental processes, including seed germination, root and stem elongation, rosette expansion, floral induction and anther development. The diverse effects of GAs on plant development make it critical to maintain an appropriate endogeno...

journal_title：Plant molecular biology

authors： Zhang Y,Schwarz S,Saedler H,Huijser P

更新日期：2007-02-01 00:00:00

abstract：:Considerable progress in tomato molecular biology has been made over the past five years. At least 19 different mRNAs which increase in amount during tomato fruit ripening have been cloned and genes for enzymes involved in cell wall degradation (polygalacturonase and pectinesterase) and ethylene synthesis (ACC synthas...

journal_title：Plant molecular biology

authors： Gray J,Picton S,Shabbeer J,Schuch W,Grierson D

更新日期：1992-05-01 00:00:00

abstract：:The DNA sequence composition of 526 dicot and 345 monocot intron sequences have been characterized using computational methods. Splice site information content and bulk intron and exon dinucleotide composition were determined. Positions 4 and 5 of 5' splice sites contain different statistically significant levels of i...

journal_title：Plant molecular biology

authors： White O,Soderlund C,Shanmugan P,Fields C

更新日期：1992-09-01 00:00:00

abstract：:Growth in the apical elongation zone of plant roots is central to the development of functional root systems. Rates of root segmental elongation change from accelerating to decelerating as cell development proceeds from newly formed to fully elongated status. One of the primary variables regulating these changes in el...

journal_title：Plant molecular biology

authors： Bassani M,Neumann PM,Gepstein S

更新日期：2004-10-01 00:00:00

abstract：KEY MESSAGE:Lack of structural components results in inflorescence stem bending. Differentially expressed genes involved in lignin and hemicellulose biosynthesis are vital; genes involved in cellulose and glycan biosynthesis are also relevant. An erect inflorescence stem is essential for high-quality cut herbaceous peo...

journal_title：Plant molecular biology

authors： Wan Y,Zhang M,Hong A,Lan X,Yang H,Liu Y

更新日期：2020-02-01 00:00:00

abstract：:Unilateral incompatibility often occurs between self-incompatible (SI) species and their self-compatible (SC) relatives. For example, SI Nicotiana alata rejects pollen from SC N. plumbaginifolia, but the reciprocal pollination is compatible. This interspecific pollen rejection system closely resembles intraspecific S-...

journal_title：Plant molecular biology

authors： Beecher B,Murfett J,McClure BA

更新日期：1998-03-01 00:00:00

abstract：:Isolation of cDNAs encoding individual members of a gene family is essential for assessing their role in a biological phenomenon. However, this process is often laborious and slow due to highly conserved protein-coding region that interferes with the isolation of the individual members. Identification of gene-specific...

journal_title：Plant molecular biology

authors： Joshi CP,Nguyen HT

更新日期：1996-06-01 00:00:00

abstract：:DNA amplification fingerprinting (DAF) using a high primer-to-template ratio and single, very short arbitrary primers, was used to generate amplified fragment length polymorphic markers (AFLP) in soybean (Glycine max (L.) Merr.). The inheritance of AFLPs was studied using a cross between the ancestral Glycine soja PI4...

journal_title：Plant molecular biology

authors： Prabhu RR,Gresshoff PM

更新日期：1994-10-01 00:00:00

abstract：:Differential screening of cDNA libraries of unfertilized egg cells and in vitro zygotes of maize resulted in the isolation of more than 50 different genes whose expression is up- or down-regulated after in vitro fertilization (IVF). Among these genes, we identified a cDNA encoding the eukaryotic translation initiation...

journal_title：Plant molecular biology

authors： Dresselhaus T,Cordts S,Lörz H

更新日期：1999-03-01 00:00:00

abstract：:An antisense approach was used to attempt to determine the function of the highly abundant, tapetum-specific A9 transcript in microsporogenesis. A Brassica napus A9 cDNA clone was linked in sense and antisense orientations to the Arabidopsis thaliana A9 promoter and the resulting chimaeric genes introduced into B. nap...

journal_title：Plant molecular biology

authors： Turgut K,Barsby T,Craze M,Freeman J,Hodge R,Paul W,Scott R

更新日期：1994-01-01 00:00:00

abstract：:Agrobacterium tumefaciens was used to genetically transform sorghum. Immature embryos of a public (P898012) and a commercial line (PHI391) of sorghum were used as the target explants. The Agrobacterium strain used was LBA4404 carrying a 'Super-binary' vector with a bar gene as a selectable marker for herbicide resista...

journal_title：Plant molecular biology

authors： Zhao ZY,Cai T,Tagliani L,Miller M,Wang N,Pang H,Rudert M,Schroeder S,Hondred D,Seltzer J,Pierce D

更新日期：2000-12-01 00:00:00

abstract：:RNA b is the most abundant member of a family of autonomously replicating single- and double-stranded RNA plasmids found in maize mitochondria. The extent to which this molecule is associated with proteins was investigated by rate zonal and CsCl equilibrium density gradient centrifugation of clarified lysates of S cyt...

journal_title：Plant molecular biology

authors： Formanová N,Brown GG

更新日期：1997-06-01 00:00:00

abstract：:The mitochondrial DNA molecules of two interfertile algal species, Chlamydomonas smithii and C. reinhardtii, are co-linear except for a 1075 bp intron (the alpha-insert) that is present in the cob gene of C. smithii. The alpha-insert, a group I intron (Cs cob.1) containing an open reading frame (ORF) which encodes a b...

journal_title：Plant molecular biology

authors： Ma DP,King YT,Kim Y,Luckett WS Jr

更新日期：1992-03-01 00:00:00

abstract：:T-DNA tagging is a high throughput strategy for identifying and cloning functional genes in plants. In this study, we screened 4416 lab-created T(1) rice T-DNA tagged lines and identified a mutant, designated dh1 (degenerated hull1), with phenotype of degenerated hull and naked pistils and stamens. Approximately 60% f...

journal_title：Plant molecular biology

authors： Li A,Zhang Y,Wu X,Tang W,Wu R,Dai Z,Liu G,Zhang H,Wu C,Chen G,Pan X

更新日期：2008-03-01 00:00:00

abstract：:The function of the secretory pathway is dependent on multiple protein-protein interactions at various stages. Currently, such interactions are mainly studied using physical methods that document direct contact or affinity in vitro. The development of vital fluorescence imaging as well as quantitative protein transpor...

journal_title：Plant molecular biology

authors： Pimpl P,Denecke J

更新日期：2002-12-01 00:00:00

abstract：:Telomere homeostasis is regulated at multiple levels, including the local chromatin structure of telomeres and subtelomeres. Recent reports demonstrated that a decrease in repressive chromatin marks, such as levels of cytosine methylation in subtelomeric regions, results in telomere elongation in mouse cells. Here we ...

journal_title：Plant molecular biology

authors： Majerová E,Fojtová M,Mozgová I,Bittová M,Fajkus J

更新日期：2011-11-01 00:00:00

abstract：:Cistus creticus subsp. creticus is a plant of intrinsic scientific interest due to the distinctive pharmaceutical properties of its resin. Labdane-type diterpenes, the main constituents of the resin, exhibit considerable antibacterial and cytotoxic activities. In this study chemical analysis of isolated trichomes from...

journal_title：Plant molecular biology

authors： Falara V,Fotopoulos V,Margaritis T,Anastasaki T,Pateraki I,Bosabalidis AM,Kafetzopoulos D,Demetzos C,Pichersky E,Kanellis AK

更新日期：2008-12-01 00:00:00

abstract：:Using a 2,4-dichlorophenoxyacetic acid (2,4-D)-dependent tobacco cell-suspension culture we analyzed early hormone-induced molecular events preceding cell division. By differential screening of a cDNA library to mRNAs derived from hormone-starved cells treated with 2,4-D for 4 h, seven non-cross hybridizing cDNA clone...

journal_title：Plant molecular biology

authors： van der Zaal EJ,Memelink J,Mennes AM,Quint A,Libbenga KR

更新日期：1987-03-01 00:00:00

abstract：:The promoter region (-309 to +44) of the Brassica napus storage protein gene napA was studied in transgenic tobacco by successive 5' as well as internal deletions fused to the reporter gene GUS (beta-glucuronidase). The expression in the two main tissues of the seed, the endosperm and the embryo, was shown to be diffe...

journal_title：Plant molecular biology

authors： Ellerström M,Stålberg K,Ezcurra I,Rask L

更新日期：1996-12-01 00:00:00

abstract：:A tapetum-specific gene, RTS, has been isolated by differential screening of a cDNA library from rice panicles. RTS is a unique gene in the rice genome. RNA blot analysis and in situ hybridization indicates that this gene is predominantly expressed in the anther's tapetum during meiosis and disappears before anthesis....

journal_title：Plant molecular biology

authors： Luo H,Lee JY,Hu Q,Nelson-Vasilchik K,Eitas TK,Lickwar C,Kausch AP,Chandlee JM,Hodges TK

更新日期：2006-10-01 00:00:00

abstract：:RNA silencing is a sequence-specific RNA degradation mechanism found in most eukaryotes, where small cleavage products (siRNAs) of double stranded RNA (dsRNA) mediate silencing of genes with sequence identity to the dsRNA inducer. In several systems, silencing has been found to spread from the dsRNA inducer sequence i...

journal_title：Plant molecular biology

authors： Petersen BO,Albrechtsen M

更新日期：2005-07-01 00:00:00

abstract：:The expression of beta-amylase genes in rice (Oryza sativa) and its regulation by phytohormones gibberellic acid (GA) and abscisic acid (ABA) were examined. Upon germination beta-amylase is synthesized de novo in aleurone cells and (GA) is not required. Exogenous addition of GA does not enhance the beta-amylase activi...

journal_title：Plant molecular biology

authors： Wang SM,Lue WL,Eimert K,Chen J

更新日期：1996-08-01 00:00:00

abstract：:Three polypeptides of 33 kD, 23 kD and 16 kD are released from maize photosystem II particles by alkaline Tris solution treatment and shown to cross-react with antisera to purified spinach oxygen-evolving (OE) polypeptides of 34 kD, 23 kD and 17 kD, respectively. They are not located exclusively in mesophyll cells but...

journal_title：Plant molecular biology

authors： Sheen JY,Sayre RT,Bogorad L

更新日期：1987-05-01 00:00:00

abstract：:In the post-genomic era, many useful tools have been developed to accelerate the investigation of gene functions. Fluorescent proteins have been widely used as protein tags for studying the subcellular localization of proteins in plants. Several fluorescent organelle marker lines have been generated in dicot plants; h...

journal_title：Plant molecular biology

authors： Wu TM,Lin KC,Liau WS,Chao YY,Yang LH,Chen SY,Lu CA,Hong CY

更新日期：2016-01-01 00:00:00

abstract：KEY MESSAGE:TaPSK5 is a less conserved target of miR164 in wheat encoding a positive regulator of root growth and yield traits that could be used for crop improvement. MicroRNAs (miRNAs) play key roles in regulating plant growth and development by targeting the mRNAs of conserved genes. However, little is known about t...

journal_title：Plant molecular biology

authors： Geng Y,Jian C,Xu W,Liu H,Hao C,Hou J,Liu H,Zhang X,Li T

更新日期：2020-12-01 00:00:00

abstract：:The sesame 2S albumin (2Salb) promoter was evaluated for its capacity to express the reporter gusA gene encoding β-glucuronidase in transgenic tobacco seeds relative to the soybean fad3C gene promoter element. Results revealed increased expression of gusA gene in tobacco seed tissue when driven by sesame 2S albumin pr...

journal_title：Plant molecular biology

authors： Bhunia RK,Chakraborty A,Kaur R,Gayatri T,Bhattacharyya J,Basu A,Maiti MK,Sen SK

更新日期：2014-11-01 00:00:00

abstract：:A leaf cDNA library from hybrid aspen, Populus tremula x tremuloides, was constructed. From this two different cDNA clones, denoted CA1a and CA1b, encoding a chloroplastic carbonic anhydrase (CA) were isolated and DNA sequenced. Analysis of the deduced amino acid sequences showed that the isolated CAs belong to the be...

journal_title：Plant molecular biology

authors： Larsson S,Björkbacka H,Forsman C,Samuelsson G,Olsson O

更新日期：1997-07-01 00:00:00

abstract：:Tremendous efforts have been taken worldwide to develop genome-wide genetic stocks for rice functional genomic (FG) research since the rice genome was completely sequenced. To facilitate FG research of complex polygenic phenotypes in rice, we report the development of over 20,000 introgression lines (ILs) in three eli...

journal_title：Plant molecular biology

authors： Li ZK,Fu BY,Gao YM,Xu JL,Ali J,Lafitte HR,Jiang YZ,Rey JD,Vijayakumar CH,Maghirang R,Zheng TQ,Zhu LH

更新日期：2005-09-01 00:00:00