Abstract：
:Unilateral incompatibility often occurs between self-incompatible (SI) species and their self-compatible (SC) relatives. For example, SI Nicotiana alata rejects pollen from SC N. plumbaginifolia, but the reciprocal pollination is compatible. This interspecific pollen rejection system closely resembles intraspecific S-allele-specific pollen rejection. However, the two systems differ in degree of specificity. In SI, rejection is S-allele-specific, meaning that only a single S-RNase causes rejection of pollen with a specific S genotype. Rejection of N. plumbaginifolia pollen is less specific, occurring in response to almost any S-RNase. Here, we have tested whether a non-S-RNase can cause rejection of N. plumbaginifolia pollen. The Escherichia coli rna gene encoding RNAseI was engineered for expression in transgenic (N. plumbaginifolia x SC N. alata) hybrids. Expression levels and pollination behavior of hybrids expressing E. coli RNaseI were compared to controls expressing SA2-RNase from N. alata. Immunoblot analysis and RNase activity assays showed that RNaseI and SA2-RNase were expressed at comparable levels. However, expression of SA2-RNase caused rejection of N. plumbaginifolia pollen, whereas expression of RNaseI did not. Thus, in this system, RNase activity alone is not sufficient for rejection of N. plumbaginifolia pollen. The results suggest that S-RNases may be specially adapted to function in pollen rejection.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Beecher B,Murfett J,McClure BAdoi
10.1023/a:1005904230755subject
Has Abstractpub_date
1998-03-01 00:00:00pages
553-63issue
4eissn
0167-4412issn
1573-5028journal_volume
36pub_type
杂志文章abstract：:In higher eukaryotes, the proliferating cell nuclear antigen (PCNA) can be found associated to Cyclin D and Cdk4/6, the kinase complex responsible for cell cycle commitment in response to growth and mitogenic signals. During maize germination, PCNA can be found in protein complexes between 131 and 163 kDa. The sizes o...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1016029001537
更新日期：2002-09-01 00:00:00
abstract：:A rice gene, OsAPT2, which encodes a putative adenine phosphoribosyl transferase (APRT), was cloned and characterized. Analysis of the cDNA and genomic sequences revealed seven exons and six introns in the OsAPT2. The deduced amino acid sequence of OsAPT2 is highly homologous to those of previously isolated APRTs. RT-...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-005-4208-5
更新日期：2006-02-01 00:00:00
abstract：KEY MESSAGE:Related motifs strongly increase gene expression when added to an intron located in coding sequences. Many introns greatly increase gene expression through a mechanism that remains elusive. An obstacle to understanding intron-mediated enhancement (IME) has been the difficulty of locating the specific intron...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0516-1
更新日期：2016-10-01 00:00:00
abstract：:To manipulate the quantity and quality of storage components in Brassica napus seeds, we have constructed an antisense gene for the storage protein napin. The antisense gene was driven by the 5'-flanking region of the B. napus napin gene to express antisense RNA in a seed-specific manner. Seeds of transgenic plants wi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040693
更新日期：1994-11-01 00:00:00
abstract：:In angiosperms the interactions between the secretory matrix of the stylar transmitting tract and the growing pollen tubes have central roles in determining a successful fertilization. Sp41 is a major glycosylated component of the soluble proteins of the transmitting tract matrix and exhibits (1-3)-beta-glucanase acti...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00014970
更新日期：1995-12-01 00:00:00
abstract：:The Guillardia theta chloroplast hlpA gene encodes a protein resembling bacterial histone-like protein HU. This gene was cloned and overexpressed in Escherichia coli cells, and the resulting protein product, HlpA, was purified and characterized in vitro. In addition to exhibiting a general DNA-binding activity, the ch...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005867215258
更新日期：1997-05-01 00:00:00
abstract：:Two hevein-like peptides from the seed of Pharbitis nil, designated Pharbitis nil antimicrobial peptide 1 (Pn-AMP1) and Pn-AMP2, had been purified previously. Both exhibit potent in vitro antifungal activity against a broad spectrum of phytopathogenic fungi. We now report the isolation of two cDNA clones, designated p...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1019864222515
更新日期：2002-10-01 00:00:00
abstract：:The beneficial root-colonizing fungus Piriformospora indica stimulates root development of Chinese cabbage (Brassica campestris subsp. Chinensis) and this is accompanied by the up-regulation of a τ-class glutathione (GSH)-S-transferase gene (BcGSTU) (Lee et al. 2011) in the roots. BcGSTU expression is further promoted...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0531-2
更新日期：2016-12-01 00:00:00
abstract：:A novel CBL-interacting protein kinase (CIPK) gene, ZmCIPK16, was isolated from maize (Zea mays), which has been certified to have two copies in the genome. The ZmCIPK16 is strongly induced in maize seedlings by PEG, NaCl, ABA, dehydration, heat and drought, but not by cold. A yeast two-hybrid assay demonstrated that ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9445-y
更新日期：2009-04-01 00:00:00
abstract：:The intrinsic chlorophyll-protein CP 47 is a component of photosystem II which functions in both light-harvesting and oxygen evolution. The large extrinsic loop E of this protein has been shown to interact with the oxygen-evolving site. Previously, Vermaas and coworkers have produced a number of deletions within loop ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00041405
更新日期：1996-12-01 00:00:00
abstract：:Approximately two-thirds of the 55 to 60 plastid ribosomal proteins are encoded in the nucleus. Since the protein products of each of these genes are needed in equal amounts for ribosome assembly, their expression may be coordinately regulated by common mechanisms. To begin to understand how the expression of these ge...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019207
更新日期：1992-03-01 00:00:00
abstract：:The bZIP transcription factor (TF) family plays an important role in the abscisic acid (ABA) signaling pathway of abiotic stress in plants. We here report the cloning and characterization of OsbZIP71, which encodes a rice bZIP TF. Functional analysis showed that OsbZIP71 is a nuclear-localized protein that specificall...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-013-0115-3
更新日期：2014-01-01 00:00:00
abstract：:Controlled cellular suicide is an important process that can be observed in various organs during plant development. From the generation of proper sexual organs in monoecious plants to the hypersensitive response (HR) that occurs during incompatible pathogen interactions, programmed cell death (PCD) can be readily obs...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006133311402
更新日期：1999-04-01 00:00:00
abstract：:A cDNA encoding the multifunctional cytochrome P450, CYP71E1, involved in the biosynthesis of the cyanogenic glucoside dhurrin from Sorghum bicolor (L.) Moench was isolated. A PCR approach based on three consensus sequences of A-type cytochromes P450- (V/I)KEX(L/F)R, FXPERF, and PFGXGRRXCXG-was applied. Three novel cy...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005915507497
更新日期：1998-02-01 00:00:00
abstract：:Starch-branching enzymes (SBE) alter starch structure by breaking an alpha-1,4 linkage and attaching the reducing end of the new chain to a glucan chain by an alpha 1,6 bond. In maize, three isoforms of SBE have been identified. In order to examine the function of the SBEI isoform, a reverse-genetics PCR-based screen ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1013335217744
更新日期：2002-02-01 00:00:00
abstract：:Micropropagated shoots of three forest tree species, poplar (Populus tremula x P. alba), wild cherry (Prunus avium L.) and walnut (Juglans nigra x J. regia), were inoculated each with six different wild-type Agrobacterium strains. Poplar and wild cherry developed tumors that grew hormone-independently, whereas on waln...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040638
更新日期：1991-09-01 00:00:00
abstract：:It has been demonstrated that insertion of foreign DNA into mammalian genome can profoundly alter the patterns of DNA methylation and transcription of the host genome. Introgression of alien DNA into plant genomes through sexual crossing and genetic engineering are commonly used in breeding, but it is not known if pla...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/B:PLAN.0000038270.48326.7a
更新日期：2004-03-01 00:00:00
abstract：:Plastids possess a bacteria-like sec apparatus that is involved in protein import into the thylakoid lumen. We have analyzed one of the genes essential for this process, secY. A secY gene from the unicellular red alga Cyanidium caldarium was found to be transcriptionally active, demonstrating for the first time that s...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020209
更新日期：1996-11-01 00:00:00
abstract：:The gene encoding nitrite reductase (nir) from the cyanobacterium Synechococcus sp. PCC 7942 has been identified and sequenced. This gene comprises 1536 nucleotides and would encode a polypeptide of 56,506 Da that shows similarity to nitrite reductase from higher plants and to the sulfite reductase hemoprotein from en...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00023618
更新日期：1993-03-01 00:00:00
abstract：:Porphobilinogen deaminase (PBG deaminase) is an early enzyme of the pathway for chlorophyll and heme synthesis. Using degenerate oligonucleotide primers, based on amino acid sequence data for purified PBG deaminase from pea, a fragment was amplified from Arabidopsis genomic DNA by PCR, and then used to isolate both a ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00028854
更新日期：1994-11-01 00:00:00
abstract：:The tumor-associated glycoprotein 72 (TAG 72) has been shown to be expressed in the majority of human adenocarcinomas. In an effort to develop a technique for the safe and inexpensive production of large quantities of anti-TAG 72 humanized antibody fragments (hzAb) as a future source of clinical-grade proteins, we dev...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9379-4
更新日期：2008-11-01 00:00:00
abstract：:Transposable elements were first discovered in plants because they can have tremendous effects on genome structure and gene function. Although only a few or no elements may be active within a genome at any time in any individual, the genomic alterations they cause can have major outcomes for a species. All major eleme...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：
更新日期：2000-01-01 00:00:00
abstract：:The shoot apical meristem of higher plants is characterized by a conserved pattern of cell division, the functional significance of which is unclear. Although a causal role for cell division frequency and orientation in morphogenesis has been suggested, supporting data are limited. An alternative interpretation laying...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1007/s11103-005-7703-9
更新日期：2006-04-01 00:00:00
abstract：:Ribose-5-phosphate isomerase (RPI) catalyses the interconversion of ribose-5-phosphate and ribulose-5-phosphate in the reductive and oxidative pentose phosphate pathways in plants. RPI from spinach chloroplasts was purified and microsequenced. Via PCR with degenerate primers designed against microsequenced peptides, a...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019012
更新日期：1996-02-01 00:00:00
abstract：:Mitochondrial maturation during imbibition of rice embryos follows the transition of unstructured double membrane bound pro-mitochondria to the typical cristae-rich mitochondrial structures observed in mature plant cells. During the first 48 h following imbibition, an ordered increase in the abundance of transcripts e...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-005-3688-7
更新日期：2006-01-01 00:00:00
abstract：:Beyond the rewards of plant genome analysis and gene identification, characterisation of protein activities, post-translational modifications and protein complex composition remains a challenge for plant biologists. Ideally, methods should allow rapid isolation of proteins from plant material achieving a high degree o...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-004-0501-y
更新日期：2004-05-01 00:00:00
abstract：:Ustilago maydis killer toxins are small polypeptides (7-14 kDa) which kill susceptible cells of closely related fungal species. The KP4 toxin is a single polypeptide subunit with a molecular weight of 11.1 kDa. In this work, a transgenic tobacco plant was constructed which secretes the KP4 toxin at a high level. The K...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020122
更新日期：1996-01-01 00:00:00
abstract：:DNA methylation plays an important role in the gene-silencing network of higher eukaryotes. We have analyzed the 21.5-kb maintenance methyltransferase (M-MTase) gene, met1, of the multicellular green alga Volvox carteri. The met1 transcript was detected only during the period when DNA replication and cell division are...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-006-9091-1
更新日期：2007-02-01 00:00:00
abstract：:In bacterial and animal coenzyme A (CoA) biosynthesis, pantothenate kinase (PANK) activity is critical in regulating intracellular CoA levels. Less is known about the role of PANK in plants, although a single plant isozyme from Arabidopsis, AtPANK1, was previously cloned and analyzed in vitro. We report here the chara...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-006-0037-4
更新日期：2006-07-01 00:00:00
abstract：:We have used a reverse transcriptase polymerase chain reaction procedure (differential display) to isolate cDNAs corresponding to transcripts that accumulate in ozone-treated Arabidopsis thaliana. In this report we describe the characterization of an ozone-induced transcript, AtOZI1. AtOZI1 mRNA in untreated plants wa...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019121
更新日期：1995-10-01 00:00:00
