Microsequencing and cDNA cloning of the Calvin cycle/OPPP enzyme ribose-5-phosphate isomerase (EC 5.3.1.6) from spinach chloroplasts.

abstract：:In wild-type Arabidopsis seeds the 2S albumin seed protein gene family members are differentially expressed. In this work it is shown that as predicted by the wild type situation, the at2S2 promoter is much more effective than that of the at2S1 gene in the expression of a transgene. However, unexpectedly high expressi...

journal_title：Plant molecular biology

authors： Conceição Ada S,Van Vliet A,Krebbers E

更新日期：1994-11-01 00:00:00

abstract：KEY MESSAGE:The expression of MdBBX10 was significantly induced by different stresses and ABA treatments. Overexpression of MdBBX10 in Arabidopsis significantly enhanced abiotic stresses tolerance by ABA signalling. The roles of B-box domain(s) containing proteins (BBXs) in regulation of flowering and light morphogenes...

journal_title：Plant molecular biology

authors： Liu X,Li R,Dai Y,Yuan L,Sun Q,Zhang S,Wang X

更新日期：2019-03-01 00:00:00

abstract：:When subjected to salt stress or drought, some vascular plants such as barley respond with an increased accumulation of the osmoprotectant glycine betaine (betaine), being the last step of betaine synthesis catalyzed by betaine aldehyde dehydrogenase (BADH). We report here cloning and characterization of BADH cDNA fro...

journal_title：Plant molecular biology

authors： Ishitani M,Nakamura T,Han SY,Takabe T

更新日期：1995-01-01 00:00:00

abstract：:Homospermidine synthase is the first specific enzyme in the biosynthesis of pyrrolizidine alkaloids. Whereas the substrates putrescine and spermidine are part of the highly dynamic polyamine pool of plants, the product homospermidine is incorporated exclusively into the necine base moiety of pyrrolizidine alkaloids. R...

journal_title：Plant molecular biology

authors： Abdelhady MI,Beuerle T,Ober D

更新日期：2009-09-01 00:00:00

abstract：:A chimeric gene consisting of the 5' flanking sequences of a rice glutelin gene (Gt3) linked to the chloramphenicol acetyltransferase (CAT) coding segment was introduced into tobacco via Agrobacterium tumefaciens-mediated transformation. CAT enzyme activity could be detected in extracts from seeds as early as 8 days a...

journal_title：Plant molecular biology

authors： Leisy DJ,Hnilo J,Zhao Y,Okita TW

更新日期：1990-01-01 00:00:00

abstract：:Two dimensional gel electrophoresis was used to examine differences in steady state total poly(A) RNA from untransformed potato (Solanum tuberosum cv. Maris Bard) and potato transformed with shoot-inducing TL-DNA from A. tumefaciens. RNA was compared from phenotypically very distinct in vitro cultured shoots, more sim...

journal_title：Plant molecular biology

authors： Burrell MM,Temple S,Ooms G

更新日期：1986-07-01 00:00:00

abstract：:Arabidopsis thaliana encodes four Dicer-like (DCL) proteins and five dsRNA-binding (DRB) proteins. We have previously demonstrated that DCL4 specifically interacts with DRB4 in vitro. Here we describe the interaction between DCL4 and DRB4 in vivo. The phenotype of a mutant with a defect in DCL4 (dcl4-2) was similar to...

journal_title：Plant molecular biology

authors： Nakazawa Y,Hiraguri A,Moriyama H,Fukuhara T

更新日期：2007-04-01 00:00:00

abstract：:Anaerobiosis rapidly induces alcohol dehydrogenase (ADH), an enzyme of the fermentation pathway, in different parts of rice seedlings. After initiation of anaerobiosis, the activity of the enzyme increases linearly for 3 days or more. The ADH activity is anaerobically inducible even in mature rice leaves in contrast t...

journal_title：Plant molecular biology

authors： Xie Y,Wu R

更新日期：1989-07-01 00:00:00

abstract：:We present evidence for a unique covalent modification of a nuclear-encoded precursor protein targeted to plant mitochondria. We investigated the early events of in vitro import for the mitochondrial precursor of the ATP synthase F1beta subunit from Nicotiana plumbaginifolia (pF1beta) into plant mitochondria. When pF1...

journal_title：Plant molecular biology

authors： von Stedingk E,Pavlov PF,Grinkevich VA,Glaser E

更新日期：1999-11-01 00:00:00

abstract：:Despite the presence in nature of many functional gene families that contain several to many highly similar sequences, the presence of identical DNA sequence repeats is widely thought to predispose transgene inserts to homology dependent gene silencing (HDGS). The induction of transcriptional gene silencing (TGS) by R...

journal_title：Plant molecular biology

authors： Yang G,Lee YH,Jiang Y,Kumpatla SP,Hall TC

更新日期：2005-06-01 00:00:00

abstract：:In this report we characterized the Arabidopsis ABI1 gene orthologue and Brassica napus gene paralogues encoding protein phosphatase 2C (PP2C, group A), which is known to be a negative regulator of the ABA signaling pathway. Six homologous B. napus sequences were identified and characterized as putative PP2C group A m...

journal_title：Plant molecular biology

authors： Babula-Skowrońska D,Ludwików A,Cieśla A,Olejnik A,Cegielska-Taras T,Bartkowiak-Broda I,Sadowski J

更新日期：2015-07-01 00:00:00

abstract：:We describe here the isolation and characterization of OsiSAP8, a member of stress Associated protein (SAP) gene family from rice characterized by the presence of A20 and AN1 type Zinc finger domains. OsiSAP8 is a multiple stress inducible gene, induced by various stresses, namely heat, cold, salt, desiccation, submer...

journal_title：Plant molecular biology

authors： Kanneganti V,Gupta AK

更新日期：2008-03-01 00:00:00

abstract：:Acyl-ACP thioesterases are involved in regulating chain termination of fatty acid biosynthesis in plant systems. Previously, acyl-ACP thioesterase purified from Brassica napus seed tissue has been shown to have a high preference for hydrolysing oleoyl-ACP. Here, oligonucleotides derived from B. napus oleoyl-ACP thioes...

journal_title：Plant molecular biology

authors： Loader NM,Woolner EM,Hellyer A,Slabas AR,Safford R

更新日期：1993-11-01 00:00:00

abstract：:Salicylic acid (SA) is a stress-induced hormone involved in the activation of defense genes. Here we analyzed the early genetic responses to SA of wild type and npr1-1 mutant Arabidopsis seedlings, using Complete Arabidopsis Transcriptome MicroArray (CATMAv2) chip. We identified 217 genes rapidly induced by SA (early ...

journal_title：Plant molecular biology

authors： Blanco F,Salinas P,Cecchini NM,Jordana X,Van Hummelen P,Alvarez ME,Holuigue L

更新日期：2009-05-01 00:00:00

abstract：:Tobacco and tomato plants were generated exhibiting insect resistance due to the introduction of modified cryIA(b) and cryIC genes of Bacillus thuringiensis. Limited modifications at selected regions of the coding sequences of both genes are sufficient to obtain resistance against Spodoptera exigua, Heliothis virescen...

journal_title：Plant molecular biology

authors： van der Salm T,Bosch D,Honée G,Feng L,Munsterman E,Bakker P,Stiekema WJ,Visser B

更新日期：1994-10-01 00:00:00

abstract：:Cistus creticus subsp. creticus is a plant of intrinsic scientific interest due to the distinctive pharmaceutical properties of its resin. Labdane-type diterpenes, the main constituents of the resin, exhibit considerable antibacterial and cytotoxic activities. In this study chemical analysis of isolated trichomes from...

journal_title：Plant molecular biology

authors： Falara V,Fotopoulos V,Margaritis T,Anastasaki T,Pateraki I,Bosabalidis AM,Kafetzopoulos D,Demetzos C,Pichersky E,Kanellis AK

更新日期：2008-12-01 00:00:00

abstract：:Plant responses to abiotic stresses are coordinated by arrays of growth and developmental programs. Phytohormones such as abscisic acid (ABA) and indole-3-acetic acid (IAA) play critical roles in developmental progresses and environmental responses through complex signalling networks. However, crosstalk between the tw...

journal_title：Plant molecular biology

authors： Du H,Wu N,Chang Y,Li X,Xiao J,Xiong L

更新日期：2013-11-01 00:00:00

abstract：:A novel rice genomic sequence encoding coding segments homologous to other metallothionein-like genes was isolated from Oryza sativa genomic library. This sequence, hereby designated as rgMT (rice genomic metallothionein-like gene), consists of two exons and one intron. From the coding sequence, it is predicted that r...

journal_title：Plant molecular biology

authors： Hsieh HM,Liu WK,Huang PC

更新日期：1995-06-01 00:00:00

abstract：:A cDNA clone was isolated by differential hybridization from a library prepared from barley leaves inoculated with the fungus Blumeria graminis f.sp. hordei (Bgh). The open reading frame of the insert (designated HvCHS2) encoded a polypeptide with 72-79% identity to chalcone synthases (CHS) and 65-68% identity to stil...

journal_title：Plant molecular biology

authors： Christensen AB,Gregersen PL,Schröder J,Collinge DB

更新日期：1998-07-01 00:00:00

abstract：:Repetitive DNA sequences comprise a large percentage of plant genomes, and their characterization provides information about both species and genome evolution. We have isolated a recombinant clone containing a highly repeated DNA element (SB92) that is homologous to ca. 0.9% of the soybean genome or about 10(5) copies...

journal_title：Plant molecular biology

authors： Vahedian M,Shi L,Zhu T,Okimoto R,Danna K,Keim P

更新日期：1995-11-01 00:00:00

abstract：:Osmium tetroxide (OsO4) reacts with the thymine residues of double-stranded DNA, but thymines that are unpaired or under torsional stress are hyperreactive. Although OsO4 hyperreactivity has been primarily utilized to identify Z-DNA structures in supercoiled plasmids, OsO4 will also identify other torsional perturbati...

journal_title：Plant molecular biology

authors： Paul AL,Ferl RJ

更新日期：1993-09-01 00:00:00

abstract：:A cDNA (WPRP1) encoding a wheat proline-rich protein has been isolated and sequenced. The amino acid composition shows 45% proline, with high levels of methionine, lysine and glutamic acid. The derived 378 residue amino acid sequence has a highly repetitive structure which is unlike those of other proline-rich protein...

journal_title：Plant molecular biology

authors： Raines CA,Lloyd JC,Chao SM,John UP,Murphy GJ

更新日期：1991-04-01 00:00:00

abstract：:The expression of a 25 kDa protein, encoded by the fused mitochondrial pcf gene, is associated with cytoplasmic male sterility (CMS) in petunia. To investigate the role of the 25 kDa protein in CMS we have transformed petunia and tobacco plants with constructs expressing a portion of the urfS sequence of the pcf cDNA ...

journal_title：Plant molecular biology

authors： Wintz H,Chen HC,Sutton CA,Conley CA,Cobb A,Ruth D,Hanson MR

更新日期：1995-04-01 00:00:00

abstract：:Despite recent progress auxin signal transduction remains largely scetchy and enigmatic. A good body of evidence supports the notion that the ABP1 could be a functional receptor or part of a receptor, respectively, but this is not generally accepted. Evidence for other functional receptors is lacking, as is any clearc...

journal_title：Plant molecular biology

authors： Scherer GF

更新日期：2002-06-01 00:00:00

abstract：:It has been demonstrated that insertion of foreign DNA into mammalian genome can profoundly alter the patterns of DNA methylation and transcription of the host genome. Introgression of alien DNA into plant genomes through sexual crossing and genetic engineering are commonly used in breeding, but it is not known if pla...

journal_title：Plant molecular biology

authors： Liu Z,Wang Y,Shen Y,Guo W,Hao S,Liu B

更新日期：2004-03-01 00:00:00

abstract：:Plants respond to phosphate (Pi) deficiency through adaptive mechanisms involving several morphological, biochemical and molecular changes. In this study, we have characterized the structure and expression of a tomato (Lycopersicon esculentum L.) phosphate starvation-induced gene (TPSI1). A 3.5 kb genomic fragment con...

journal_title：Plant molecular biology

authors： Liu C,Muchhal US,Raghothama KG

更新日期：1997-03-01 00:00:00

abstract：:A number of A/T-rich sequences and a CATGCAT/A sequence are contained in the 5'-upstream regions of the genes encoding concanavalin A (Con A) and canavalin, two major seed storage proteins of Canavalia gladiata D.C. To study the role of these sequences in the seed-specific gene expression, we constructed 5'-deletion m...

journal_title：Plant molecular biology

authors： Yamamoto S,Nishihara M,Morikawa H,Yamauchi D,Minamikawa T

更新日期：1995-02-01 00:00:00

abstract：:Ustilago maydis killer toxins are small polypeptides (7-14 kDa) which kill susceptible cells of closely related fungal species. The KP4 toxin is a single polypeptide subunit with a molecular weight of 11.1 kDa. In this work, a transgenic tobacco plant was constructed which secretes the KP4 toxin at a high level. The K...

journal_title：Plant molecular biology

authors： Park CM,Berry JO,Bruenn JA

更新日期：1996-01-01 00:00:00

abstract：KEY MESSAGE:This manuscript describes the functions of an Argonaute protein named AGO17 in rice. AGO17 is required for the development of rice reproductive tissues. Argonaute (AGO) proteins are a well-conserved multigene family of regulators mediating gene silencing across eukaryotes. Monocot plants have additional mem...

journal_title：Plant molecular biology

authors： Pachamuthu K,Swetha C,Basu D,Das S,Singh I,Sundar VH,Sujith TN,Shivaprasad PV

更新日期：2021-01-01 00:00:00

abstract：:Approximately two-thirds of the 55 to 60 plastid ribosomal proteins are encoded in the nucleus. Since the protein products of each of these genes are needed in equal amounts for ribosome assembly, their expression may be coordinately regulated by common mechanisms. To begin to understand how the expression of these ge...

journal_title：Plant molecular biology

authors： Thompson MD,Jacks CM,Lenvik TR,Gantt JS

更新日期：1992-03-01 00:00:00