Osmium tetroxide footprinting of a scaffold attachment region in the maize Adh1 promoter.

Abstract:

:Osmium tetroxide (OsO4) reacts with the thymine residues of double-stranded DNA, but thymines that are unpaired or under torsional stress are hyperreactive. Although OsO4 hyperreactivity has been primarily utilized to identify Z-DNA structures in supercoiled plasmids, OsO4 will also identify other torsional perturbations of DNA. In this study, OsO4 was used to footprint an AT-rich region (between -780 and -500) of the maize Adh1 promoter. Hyperreactive sites were identified both in vitro and in vivo in an area that coincides with AT motifs similar to those found in scaffold attachment regions. Further, the region of OsO4 hyperreactivity lies within a fragment of DNA that is associated with the nuclear scaffold in histone-depleted nuclei.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Paul AL,Ferl RJ

doi

10.1007/BF00028983

subject

Has Abstract

pub_date

1993-09-01 00:00:00

pages

1145-51

issue

6

eissn

0167-4412

issn

1573-5028

journal_volume

22

pub_type

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