Abstract：
:Approximately two-thirds of the 55 to 60 plastid ribosomal proteins are encoded in the nucleus. Since the protein products of each of these genes are needed in equal amounts for ribosome assembly, their expression may be coordinately regulated by common mechanisms. To begin to understand how the expression of these genes is regulated, we have isolated cDNA and genomic clones for three plastid ribosomal protein genes from an Arabidopsis thaliana library. The genes rps17, rpl9 and rpl15, encoding plastid ribosomal proteins CS17, CL9 and CL15, respectively, are located in the nuclear genome and Southern blot data suggest that each is a single copy gene in A. thaliana. Northern blot data show that transcripts from rps17, rpl9 and rpl15 are much more abundant in leaves and stems than they are in roots. The nucleotide sequences of each of these three genes were determined and their transcriptional initiation sites identified. rps17 transcripts have multiple 5' ends suggesting that they are initiated at multiple sites or are post-transcriptionally processed at their 5' end. rpl9 and rpl15 apparently have unique transcriptional initiation sites but are post-transcriptionally processed to remove six and three introns, respectively, from their primary transcripts. We have examined the genomic sequences for motifs that may be important for the proper expression of these genes. A 7 bp sequence motif, whose consensus is 5'-AGGCCCA-3', flanked by AT-rich regions was identified between 38 and 73 nucleotides upstream of the rps17, rpl9 and rpl15 transcriptional initiation sites.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Thompson MD,Jacks CM,Lenvik TR,Gantt JSdoi
10.1007/BF00019207subject
Has Abstractpub_date
1992-03-01 00:00:00pages
931-44issue
5eissn
0167-4412issn
1573-5028journal_volume
18pub_type
杂志文章abstract：:The location on the wheat chloroplast DNA map and the nucleotide sequences of the genes coding for tRNA GCC (Gly) (trnG-GCC), tRNA GUC (Asp) (trnD-GUC) and tRNA GCA (Cys) (trnC-GCA) have been determined. These three genes are located in the large single copy region of the chloroplast genome, about half-way between one...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF02418249
更新日期：1985-09-01 00:00:00
abstract：:We have isolated full-size cDNA sequences encoding the photosynthetic isoform of pyruvate orthophosphate dikinase (PPDK) of the C3 plant Flaveria pringlei. The encoded protein shares 96% identical amino acid residues with the C4 isoform of PPDK in the C4 species F. trinervia. The differing amino acid residues are even...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00013761
更新日期：1994-10-01 00:00:00
abstract：:In animals and fungi, small cysteine-rich proteins called metallothioneins (MTs) play a role in heavy metal tolerance. MT genes have been isolated in plants, but their function remains to be elucidated. We have isolated two distinct Vicia faba MT genes that belong to the type 1 group of plant MT genes in contrast to a...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005790927581
更新日期：1997-03-01 00:00:00
abstract：:Plants that utilize the highly efficient C4 photosynthetic pathway possess two types of specialized leaf cells, the mesophyll and bundle sheath. In mature leaves of amaranth, a dicotyledonous C4 plant, ribulose 1,5-bisphosphate carboxylase (Rubisco) is localized specifically to the chloroplasts of bundle sheath cells,...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00015971
更新日期：1993-06-01 00:00:00
abstract：:Cyclins are involved in the regulation of cell cycle progression in eukaryotes. We have isolated a cyclin cDNA clone, cycZm2w, from maize root tip cells, which fits best into group A2 of current plant cyclin gene classification schemes. The cDNA encodes a protein with a domain homologous to the cyclin box of mitotic c...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005968901298
更新日期：1998-05-01 00:00:00
abstract：:This study describes the expression of a sucrose carrier at various developmental stages in Ricinus communis. A partial-length cDNA clone, RcSUT1, was isolated by RT-PCR from Ricinus seedling RNA. This is almost identical to a sucrose carrier cDNA, Rscr1, which has previously been isolated by library screening. Howeve...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006040306581
更新日期：1998-10-01 00:00:00
abstract：:We have characterized a gene (Ltp1) encoding a barley lipid transfer protein. Northern blot analysis showed that Ltp1 mRNA accumulates specifically in the aleurone layer of developing and germinating seeds. Southern blot analysis indicated that LTP1 protein is encoded by a single gene in barley. Sequence analysis of L...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040674
更新日期：1992-02-01 00:00:00
abstract：:Gibberellins (GAs) are important plant growth regulators, regulating many plant developmental processes, including seed germination, root and stem elongation, rosette expansion, floral induction and anther development. The diverse effects of GAs on plant development make it critical to maintain an appropriate endogeno...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-006-9099-6
更新日期：2007-02-01 00:00:00
abstract：:Previous study showed that the magnesium-protoporphyrin IX chelatase H subunit (CHLH/ABAR) positively regulates abscisic acid (ABA) signaling. Here, we investigated the functions of a CHLH/ABAR interaction protein, the chloroplast co-chaperonin 20 (CPN20) in ABA signaling in Arabidopsis thaliana. We showed that down-e...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-013-0082-8
更新日期：2013-10-01 00:00:00
abstract：:One hundred maize zygotic embryos microdissected at the transition stage were used to construct a cDNA library after non-selective PCR (NS-PCR) amplification of whole cDNA populations. The library contains 2.3 x 10(5) recombinants and two different calmodulin cDNAs were cloned using a heterologous probe from petunia. ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019182
更新日期：1995-01-01 00:00:00
abstract：:Binary Ti vectors are the plasmid vectors of choice in Agrobacterium-mediated plant transformation protocols. The pGreen series of binary Ti vectors are configured for ease-of-use and to meet the demands of a wide range of transformation procedures for many plant species. This plasmid system allows any arrangement of ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006496308160
更新日期：2000-04-01 00:00:00
abstract：:A cDNA with sequence similarity to isocitrate lyase (ICL) genes was isolated from the unicellular eukaryotic green alga Chlamydomonas reinhardtii as a light-induced mRNA in the carotenoid biosynthetic mutant strain FN68. The 416 amino acid open reading frame shows significant sequence similarity to isocitrate lyases o...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005728411921
更新日期：1997-02-01 00:00:00
abstract：:A cDNA encoding CYP79B1 has been isolated from Sinapis alba. CYP79B1 from S. alba shows 54% sequence identity and 73% similarity to sorghum CYP79A1 and 95% sequence identity to the Arabidopsis T42902, assigned CYP79B2. The high identity and similarity to sorghum CYP79A1, which catalyses the conversion of tyrosine to p...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006064202774
更新日期：1998-11-01 00:00:00
abstract：:Transposable elements were first discovered in plants because they can have tremendous effects on genome structure and gene function. Although only a few or no elements may be active within a genome at any time in any individual, the genomic alterations they cause can have major outcomes for a species. All major eleme...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：
更新日期：2000-01-01 00:00:00
abstract：:The LI818 gene from Chlamydomonas encodes a polypeptide that is related to the chlorophyll a/b-binding proteins (CAB) of higher plants and green algae. However, despite this relatedness, LI818 gene expression is not coordinated with that of cab genes and is regulated differently by light, suggesting a different role f...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006340308077
更新日期：2000-01-01 00:00:00
abstract：:A cDNA clone was isolated by differential hybridization from a library prepared from barley leaves inoculated with the fungus Blumeria graminis f.sp. hordei (Bgh). The open reading frame of the insert (designated HvCHS2) encoded a polypeptide with 72-79% identity to chalcone synthases (CHS) and 65-68% identity to stil...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006031822141
更新日期：1998-07-01 00:00:00
abstract：:The expression of a number of plant genes is regulated by an endogenous circadian clock. We report that the Arabidopsis NIA2 (nitrate reductase) gene shows robust circadian oscillations in mRNA accumulation which persist for at least 5 days in plants that have been grown in a light-dark (LD) cycle and then transformed...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00029010
更新日期：1993-10-01 00:00:00
abstract：:Mitochondrial maturation during imbibition of rice embryos follows the transition of unstructured double membrane bound pro-mitochondria to the typical cristae-rich mitochondrial structures observed in mature plant cells. During the first 48 h following imbibition, an ordered increase in the abundance of transcripts e...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-005-3688-7
更新日期：2006-01-01 00:00:00
abstract：:In higher eukaryotes, the proliferating cell nuclear antigen (PCNA) can be found associated to Cyclin D and Cdk4/6, the kinase complex responsible for cell cycle commitment in response to growth and mitogenic signals. During maize germination, PCNA can be found in protein complexes between 131 and 163 kDa. The sizes o...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1016029001537
更新日期：2002-09-01 00:00:00
abstract：:Tremendous efforts have been taken worldwide to develop genome-wide genetic stocks for rice functional genomic (FG) research since the rice genome was completely sequenced. To facilitate FG research of complex polygenic phenotypes in rice, we report the development of over 20,000 introgression lines (ILs) in three eli...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-005-8519-3
更新日期：2005-09-01 00:00:00
abstract：:RNA editing is one of the post-transcriptional processes that commonly occur in plant plastids and mitochondria. In Arabidopsis, 34 C-to-U RNA editing events, affecting transcripts of 18 plastid genes, have been identified. Here, we examined the editing and expression of these transcripts in different organs, and in g...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-013-0069-5
更新日期：2013-07-01 00:00:00
abstract：:The Arabidopsis ga1 mutant has very low levels of endogenous, active gibberellins and thus has an extreme dwarf phenotype; application of GA3 induces stem elongation and flower development. To test the hypothesis that GA action in this system involves changes in gene expression, we have cloned mRNAs whose abundance ch...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00023557
更新日期：1994-02-01 00:00:00
abstract：:Pyruvate orthophosphate dikinase (PPDK) is a critical enzyme for C(4) photosynthesis, providing the primary acceptor for fixation of bicarbonate in mesophyll cells. Although first isolated in C(4) plants, it is also present in C(3) species. We report that the single gene encoding PPDK in Arabidopsis thaliana possesses...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-006-9023-0
更新日期：2006-10-01 00:00:00
abstract：KEY MESSAGE:The crop yield losses induced by phytoviruses are mainly associated with the symptoms of the disease. DNA modifications as methylation can modulate the information coded by the sequence, process named epigenetics. Viral infection can change the expression patterns of different genes linked to defenses and s...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-020-01051-6
更新日期：2020-11-01 00:00:00
abstract：:We isolated and characterized different classes of transposable DNA elements in oil palm (Elaeis guineensis) plants grown from seed, and plants regenerated from tissue culture that show mantling, an abnormality leading to flower abortion. Using PCR assays, reverse transcriptase fragments belonging to LINE-like and gyp...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1023942309092
更新日期：2003-05-01 00:00:00
abstract：:A 1023 bp fragment and truncated derivatives of the maize (Zea mays L.) histone H3C4 gene promoter were fused to the beta-glucuronidase (GUS) gene and introduced via Agrobacterium tumefaciens into the genome of Arabidopsis thaliana. GUS activity was found in various meristems of transgenic plants as for other plant hi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005909703600
更新日期：1998-05-01 00:00:00
abstract：:Elucidating the regulatory mechanisms of plant organ formation is an important component of plant developmental biology and will be useful for crop improvement applications. Plant organ formation, or organogenesis, occurs when a group of primordial cells differentiates into an organ, through a well-orchestrated series...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-006-0054-3
更新日期：2006-08-01 00:00:00
abstract：:Ferulate 5-hydroxylase (F5H) of the monolignol pathway catalyzes the hydroxylation of coniferyl alcohol, coniferaldehyde and ferulic acid to produce 5-hydroxyconiferyl moieties, which lead to the formation of sinapic acid and syringyl (S) lignin monomers. In contrast, guaiacyl (G) lignin, the other major type of ligni...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-020-00991-3
更新日期：2020-06-01 00:00:00
abstract：KEY MESSAGE:Degradation of nitrogen-rich purines is tightly and oppositely regulated under drought and low nitrogen supply in bread wheat. Allantoin is a key target metabolite for improving nitrogen homeostasis under stress. The metabolite allantoin is an intermediate of the catabolism of purines (components of nucleot...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-019-00831-z
更新日期：2019-03-01 00:00:00
abstract：:Eukaryotes express several cytoplasmic HSP70 genes, and their encoded proteins participate in diverse cellular processes. Three cDNAs encoding highly expressed cytoplasmic HSP70 homologues from Pisum sativum were cloned and characterized. They were designated PsHSP71.2, PsHSC71.0, and PsHSP70b. These HSP70 genes have ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019312
更新日期：1995-02-01 00:00:00
