Rice alcohol dehydrogenase genes: anaerobic induction, organ specific expression and characterization of cDNA clones.

abstract：:The expression of beta-amylase genes in rice (Oryza sativa) and its regulation by phytohormones gibberellic acid (GA) and abscisic acid (ABA) were examined. Upon germination beta-amylase is synthesized de novo in aleurone cells and (GA) is not required. Exogenous addition of GA does not enhance the beta-amylase activi...

journal_title：Plant molecular biology

authors： Wang SM,Lue WL,Eimert K,Chen J

更新日期：1996-08-01 00:00:00

abstract：:It has been known for more than a decade that increasing the gene copy number does not necessarily lead to increased gene activity. Plants have developed efficient mechanisms such as post-transcriptional gene silencing (PTGS) to regulate abnormal gene expression in a sequence-specific fashion. PTGS of (trans)genes can...

journal_title：Plant molecular biology

authors： Morel JB,Vaucheret H

更新日期：2000-06-01 00:00:00

abstract：:Cosmid binary vectors for the introduction of DNA into plant cells have been constructed. These vectors are derived from the replicon of the broad host range plasmid pRK2 and contain the T-DNA border regions between which have been placed a chimaeric gene conferring resistance to kanamycin in plant cells. Appropriate ...

journal_title：Plant molecular biology

authors： van den Elzen P,Lee KY,Townsend J,Bedbrook J

更新日期：1985-05-01 00:00:00

abstract：KEY MESSAGE:Cloning of two isoamylase genes, MeISA1 and MeISA2, from cassava (Manihot esculenta Crantz) tubers, accompanied by their co-expression in E. coli demonstrates a requirement for heteromeric complex formation to achieve debranching activity. Starch debranching enzyme (DBE) or isoamylase (ISA) (EC.3.2.1.68), a...

journal_title：Plant molecular biology

authors： Panpetch P,Field RA,Limpaseni T

更新日期：2018-03-01 00:00:00

abstract：:The mitochondrial DNA molecules of two interfertile algal species, Chlamydomonas smithii and C. reinhardtii, are co-linear except for a 1075 bp intron (the alpha-insert) that is present in the cob gene of C. smithii. The alpha-insert, a group I intron (Cs cob.1) containing an open reading frame (ORF) which encodes a b...

journal_title：Plant molecular biology

authors： Ma DP,King YT,Kim Y,Luckett WS Jr

更新日期：1992-03-01 00:00:00

abstract：:Leaves develop as flat lateral organs from the indeterminate shoot apical meristem. The establishment of polarity along three-dimensional axes, proximal-distal, medial-lateral, and adaxial-abaxial axes, is crucial for the growth of normal leaves. The mutations of ASYMMETRIC LEAVES1 (AS1) and AS2 of Arabidopsis thalian...

journal_title：Plant molecular biology

authors： Nakagawa A,Takahashi H,Kojima S,Sato N,Ohga K,Cha BY,Woo JT,Nagai K,Horiguchi G,Tsukaya H,Machida Y,Machida C

更新日期：2012-08-01 00:00:00

abstract：:In previous studies we have identified several mRNAs which accumulate after addition of 2,4-dichlorophenoxyacetic-acid (2,4-D) to auxin-starved tobacco cells. The mRNAs corresponding to cDNA clone pCNT103 were found to accumulate transiently prior to the cell division response due to auxin treatment. In this study we ...

journal_title：Plant molecular biology

authors： van der Zaal EJ,Droog FN,Boot CJ,Hensgens LA,Hoge JH,Schilperoort RA,Libbenga KR

更新日期：1991-06-01 00:00:00

abstract：KEY MESSAGE:Lack of structural components results in inflorescence stem bending. Differentially expressed genes involved in lignin and hemicellulose biosynthesis are vital; genes involved in cellulose and glycan biosynthesis are also relevant. An erect inflorescence stem is essential for high-quality cut herbaceous peo...

journal_title：Plant molecular biology

authors： Wan Y,Zhang M,Hong A,Lan X,Yang H,Liu Y

更新日期：2020-02-01 00:00:00

abstract：:The relative amounts of some chloroplast polypeptides were followed during greening of leaves from three different plant families. Oat, bean and spinach were the representatives of the Gramineae, Leguminosae and Chenopodiaceae, respectively. By using specific antibodies against subunits of the chloroplast protein comp...

journal_title：Plant molecular biology

authors： Nechushtai R,Nelson N

更新日期：1985-11-01 00:00:00

abstract：:Rice provides a staple source of energy, protein and other nutrients to half of the world population. Over 90 of the rice seeds consists of starch and protein by dry weight. The quantity and property of starch and protein thus play a dominant role in the yield and quality of rice. The amylase content of starch is a de...

journal_title：Plant molecular biology

authors： Duan M,Sun SS

更新日期：2005-09-01 00:00:00

abstract：:The development of an efficient transformation system is a prerequisite for the molecular analysis of gene expression in plants. In crop plants, this development has been hindered by difficulties encountered both in whole plant regeneration from protoplasts and in the general insusceptibility of monocots to Agrobacter...

journal_title：Plant molecular biology

authors： Wang YC,Klein TM,Fromm M,Cao J,Sanford JC,Wu R

更新日期：1988-07-01 00:00:00

abstract：:The identification of some nodule-specific host proteins (nodulins) from common bean (Phaseolus vulgaris L.), a tropical ureide-transporting legume, is described. Particularly, the existence and developmental expression of several abundant nodule-specific transcripts of P. vulgaris are shown, including leghemoglobin, ...

journal_title：Plant molecular biology

authors： Campos F,Padilla J,Vázquez M,Ortega JL,Enríquez C,Sánchez F

更新日期：1987-11-01 00:00:00

abstract：:As part of a strategy to determine the precise role of pea (Pisum sativum) lectin, Psl, in nodulation of pea by Rhizobium leguminosarum, mutations were introduced into the genetic determinant for pea lectin by site-directed mutagenesis using PCR. Introduction of a specific mutation, N125D, into a central area of the s...

journal_title：Plant molecular biology

authors： van Eijsden RR,Hoedemaeker FJ,Díaz CL,Lugtenberg BJ,de Pater BS,Kijne JW

更新日期：1992-12-01 00:00:00

abstract：:A cDNA clone encoding Arabidopsis thaliana galactokinase was fortuitously isolated during the course of a screen for plant homologues of a Saccharomyces cerevisiae peroxisome assembly gene, PAS9. Clones were sought which restored the ability of pas9 cells to grow on oleate as a sole carbon source, as oleate metabolism...

journal_title：Plant molecular biology

authors： Kaplan CP,Tugal HB,Baker A

更新日期：1997-06-01 00:00:00

abstract：:As part of a strategy to define differences in genome organization and expression between cytoplasmic male-sterile (CMS) and male-fertile (MF) sugar-beet mitochondria, cDNA libraries from both mitochondrial genotypes were constructed. Preliminary screening with ribosomal RNA gene probes identified candidate cDNA clone...

journal_title：Plant molecular biology

authors： Xue Y,Collin S,Davies DR,Thomas CM

更新日期：1994-04-01 00:00:00

abstract：:Chimeric genes composed of the beta-phaseolin promoter, an alpha-zein coding sequence and its modified versions containing lysine codons, and a beta-zein polyadenylation signal were inserted into the genome of tobacco by Agrobacterium-mediated transformation. alpha-Zein mRNA levels in the transgenic tobacco seeds 20 d...

journal_title：Plant molecular biology

authors： Ohtani T,Galili G,Wallace JC,Thompson GA,Larkins BA

更新日期：1991-01-01 00:00:00

abstract：:An approximately 247-kb genomic region from FF genome of wild rice Oryza brachyantha, possessing the smallest Oryza genome, was compared to the orthologous approximately 450-kb region from AA genome, O. sativa L. ssp. japonica. 37 of 38 genes in the orthologous regions are shared between japonica and O. brachyantha. A...

journal_title：Plant molecular biology

authors： Zhang S,Gu YQ,Singh J,Coleman-Derr D,Brar DS,Jiang N,Lemaux PG

更新日期：2007-07-01 00:00:00

abstract：:Rice has developed several morphological and physiological strategies to adapt to phosphate starvation in the soil. In order to elucidate the molecular basis of response to phosphate starvation, we performed mRNA sequencing of 4 rice cultivars with variation in growth response to Pi starvation as indicated by the shoo...

journal_title：Plant molecular biology

authors： Oono Y,Kawahara Y,Yazawa T,Kanamori H,Kuramata M,Yamagata H,Hosokawa S,Minami H,Ishikawa S,Wu J,Antonio B,Handa H,Itoh T,Matsumoto T

更新日期：2013-12-01 00:00:00

abstract：:A 7.4 kb segment of a Euglena nuclear gene encoding a portion of the polyprotein precursor to the light-harvesting chlorophyll a/b-binding protein of photosystem II (LHCPII) has been isolated and sequenced. Nine exons can be assembled into a continuous open reading frame encoding 113 amino acids of the C-terminus of a...

journal_title：Plant molecular biology

authors： Muchhal US,Schwartzbach SD

更新日期：1992-01-01 00:00:00

abstract：:Chimeric genes containing the coding sequence for bacterial chloramphenicol acetyl transferase (CAT) have been introduced by electroporation into maize protoplasts (Black Mexican Sweet) and transient expression monitored by enzyme assays. Levels of CAT expression were enhanced 12-fold and 20-fold respectively by the i...

journal_title：Plant molecular biology

authors： Mascarenhas D,Mettler IJ,Pierce DA,Lowe HW

更新日期：1990-12-01 00:00:00

abstract：:The Arabidopsis ga1 mutant has very low levels of endogenous, active gibberellins and thus has an extreme dwarf phenotype; application of GA3 induces stem elongation and flower development. To test the hypothesis that GA action in this system involves changes in gene expression, we have cloned mRNAs whose abundance ch...

journal_title：Plant molecular biology

authors： Phillips AL,Huttly AK

更新日期：1994-02-01 00:00:00

abstract：:Plants that utilize the highly efficient C4 photosynthetic pathway possess two types of specialized leaf cells, the mesophyll and bundle sheath. In mature leaves of amaranth, a dicotyledonous C4 plant, ribulose 1,5-bisphosphate carboxylase (Rubisco) is localized specifically to the chloroplasts of bundle sheath cells,...

journal_title：Plant molecular biology

authors： Boinski JJ,Wang JL,Xu P,Hotchkiss T,Berry JO

更新日期：1993-06-01 00:00:00

abstract：:We report the successful combination of mRNA differential-display reverse-transcription PCR (DDRT-PCR) and 5'-rapid amplification of cDNA ends (5'-RACE) in order to isolate full-length cDNAs corresponding to genes activated in tobacco cells treated with cryptogein within 60 min. Cloning and sequencing of two cDNAs, ca...

journal_title：Plant molecular biology

authors： Petitot AS,Blein JP,Pugin A,Suty L

更新日期：1997-10-01 00:00:00

abstract：:Salicylic acid (SA) is known to play an important role in the interaction between plant and micro-organisms, both symbiotic and pathogen. In particular, high levels of SA block nodule formation and mycorrhizal colonization in plants. A mutant of Lotus japonicus, named Ljsym4-2, was characterized as unable to establish...

journal_title：Plant molecular biology

authors： Bastianelli F,Costa A,Vescovi M,D'Apuzzo E,Zottini M,Chiurazzi M,Lo Schiavo F

更新日期：2010-03-01 00:00:00

abstract：:Left- and right-border repeats, which surround the T-region, contain two conserved regions separated by 5 bp that are not conserved. At the onset of T-DNA processing virD-encoded proteins introduce a nick in the largest of these conserved regions (12 bp) at a specific position in the bottom strand between a guanine an...

journal_title：Plant molecular biology

authors： van Haaren MJ,Sedee NJ,de Boer HA,Schilperoort RA,Hooykaas PJ

更新日期：1989-11-01 00:00:00

abstract：:Due to an unfortunate turn of events, an incorrect note was provided in the original publication as it should have read. ...

journal_title：Plant molecular biology

authors： Nugroho ABD,Han N,Pervitasari AN,Kim DH,Kim J

更新日期：2020-03-01 00:00:00

abstract：:In this survey of 5074 plant genes for their AUG context sequences, purines are present at the -3 and +4 positions in about 80% of the sequences. Although this observation is similar to the vertebrate consensus sequence, the number of plant mRNAs with purines at the -3 position is lower and at the +4 position is highe...

journal_title：Plant molecular biology

authors： Joshi CP,Zhou H,Huang X,Chiang VL

更新日期：1997-12-01 00:00:00

abstract：KEY MESSAGE:Degradation of nitrogen-rich purines is tightly and oppositely regulated under drought and low nitrogen supply in bread wheat. Allantoin is a key target metabolite for improving nitrogen homeostasis under stress. The metabolite allantoin is an intermediate of the catabolism of purines (components of nucleot...

journal_title：Plant molecular biology

authors： Casartelli A,Melino VJ,Baumann U,Riboni M,Suchecki R,Jayasinghe NS,Mendis H,Watanabe M,Erban A,Zuther E,Hoefgen R,Roessner U,Okamoto M,Heuer S

更新日期：2019-03-01 00:00:00

abstract：:Homospermidine synthase is the first specific enzyme in the biosynthesis of pyrrolizidine alkaloids. Whereas the substrates putrescine and spermidine are part of the highly dynamic polyamine pool of plants, the product homospermidine is incorporated exclusively into the necine base moiety of pyrrolizidine alkaloids. R...

journal_title：Plant molecular biology

authors： Abdelhady MI,Beuerle T,Ober D

更新日期：2009-09-01 00:00:00

abstract：:The multiple copies of the chloroplast genome (plastome) are condensed and organized into nucleoids by a set of proteins. One of these, the DNA-binding protein DCP68 from soybean, has previously been shown to compact DNA and to inhibit DNA synthesis in vitro. N-terminal amino acid analysis and the absorption spectrum ...

journal_title：Plant molecular biology

authors： Chi-Ham CL,Keaton MA,Cannon GC,Heinhorst S

更新日期：2002-08-01 00:00:00