New insights into Oryza genome evolution: high gene colinearity and differential retrotransposon amplification.

abstract：:Chemically inducible gene switches can provide precise control over gene expression, enabling more specific analyses of gene function and expanding the plant biotechnology toolkit beyond traditional constitutive expression systems. The alc gene expression system is one of the most promising chemically inducible gene s...

journal_title：Plant molecular biology

authors： Kinkema M,Geijskes RJ,Shand K,Coleman HD,De Lucca PC,Palupe A,Harrison MD,Jepson I,Dale JL,Sainz MB

更新日期：2014-03-01 00:00:00

abstract：KEY MESSAGE:Cloning of two isoamylase genes, MeISA1 and MeISA2, from cassava (Manihot esculenta Crantz) tubers, accompanied by their co-expression in E. coli demonstrates a requirement for heteromeric complex formation to achieve debranching activity. Starch debranching enzyme (DBE) or isoamylase (ISA) (EC.3.2.1.68), a...

journal_title：Plant molecular biology

authors： Panpetch P,Field RA,Limpaseni T

更新日期：2018-03-01 00:00:00

abstract：:This study describes the expression of a sucrose carrier at various developmental stages in Ricinus communis. A partial-length cDNA clone, RcSUT1, was isolated by RT-PCR from Ricinus seedling RNA. This is almost identical to a sucrose carrier cDNA, Rscr1, which has previously been isolated by library screening. Howeve...

journal_title：Plant molecular biology

authors： Bick JA,Neelam A,Smith E,Nelson SJ,Hall JL,Williams LE

更新日期：1998-10-01 00:00:00

abstract：:Glutelin is the most abundant storage protein in rice, which is expressed specifically in the endosperm of maturing seed. Glutelin is encoded by about 10 genes per haploid genome, which are clearly divided into two subfamilies (GluA and GluB). Most of them are coordinately expressed during seed maturation in spite of ...

journal_title：Plant molecular biology

authors： Takaiwa F,Yamanouchi U,Yoshihara T,Washida H,Tanabe F,Kato A,Yamada K

更新日期：1996-03-01 00:00:00

abstract：:Primer extension was used to characterize alpha-amylase mRNAs from aleurone tissue of barley (Hordeum vulgare L. cv. Himalaya) grains. Two synthetic oligonucleotides, specific for the low-pI and high-pI alpha-amylase groups, were used as primers for synthesis of cDNA from total RNA preparations. Between them, these tw...

journal_title：Plant molecular biology

authors： Chandler PM,Huiet L

更新日期：1991-04-01 00:00:00

abstract：:Most plant cells retain the capacity to differentiate into all the other cell and organ types that constitute a plant. However, genome-wide transcriptional activities underlying the process of cell differentiation are poorly understood, especially in monocot plants. Here we used a rice (Oryza sativa) cell culture syst...

journal_title：Plant molecular biology

authors： Su N,He K,Jiao Y,Chen C,Zhou J,Li L,Bai S,Li X,Deng XW

更新日期：2007-02-01 00:00:00

abstract：:Porphobilinogen deaminase (PBG deaminase) is an early enzyme of the pathway for chlorophyll and heme synthesis. Using degenerate oligonucleotide primers, based on amino acid sequence data for purified PBG deaminase from pea, a fragment was amplified from Arabidopsis genomic DNA by PCR, and then used to isolate both a ...

journal_title：Plant molecular biology

authors： Lim SH,Witty M,Wallace-Cook AD,Ilag LI,Smith AG

更新日期：1994-11-01 00:00:00

abstract：:The expression of a 25 kDa protein, encoded by the fused mitochondrial pcf gene, is associated with cytoplasmic male sterility (CMS) in petunia. To investigate the role of the 25 kDa protein in CMS we have transformed petunia and tobacco plants with constructs expressing a portion of the urfS sequence of the pcf cDNA ...

journal_title：Plant molecular biology

authors： Wintz H,Chen HC,Sutton CA,Conley CA,Cobb A,Ruth D,Hanson MR

更新日期：1995-04-01 00:00:00

abstract：:cDNA clones were isolated and sequenced that encode two related but distinct rice cyclophilins, Cyp1 and Cyp2. The predicted amino acid sequences of each are 72% identical to human T-cell cyclophilin. Genomic DNA gel blot analysis suggests cyclophilins in rice are encoded by a small, 6-10-member gene family. Both Cyp1...

journal_title：Plant molecular biology

authors： Buchholz WG,Harris-Haller L,DeRose RT,Hall TC

更新日期：1994-08-01 00:00:00

abstract：:The gene encoding nitrite reductase (nir) from the cyanobacterium Synechococcus sp. PCC 7942 has been identified and sequenced. This gene comprises 1536 nucleotides and would encode a polypeptide of 56,506 Da that shows similarity to nitrite reductase from higher plants and to the sulfite reductase hemoprotein from en...

journal_title：Plant molecular biology

authors： Luque I,Flores E,Herrero A

更新日期：1993-03-01 00:00:00

abstract：:Two types of structurally distinct O-methyltransferases mediate the methylation of hydroxylated monomeric lignin precursors in angiosperms. Caffeate 3-O-methyltransferase (COMT; EC 2.1.1.68) methylates the free acids and caffeoyl CoA 3-O-methyltransferase (CCoAOMT; EC 2.1.1.104) methylates coenzyme A esters. Recently,...

journal_title：Plant molecular biology

authors： Li L,Osakabe Y,Joshi CP,Chiang VL

更新日期：1999-07-01 00:00:00

abstract：:A partial cDNA clone (PSnir) encoding the C-terminal region of nitrite reductase was isolated from a lambda gt11 library of the gymnosperm Pimus sylvestris (L.). Nucleotide sequence analysis showed that PSnir contains a reading frame encoding 105 amino acid residues. The amino acid sequence revealed a homology to NiR ...

journal_title：Plant molecular biology

authors： Neininger A,Seith B,Hoch B,Mohr H

更新日期：1994-06-01 00:00:00

abstract：:Cold acclimation in plants is a polygenic phenomenon involving increased expression of several genes. The gene products participate either directly or indirectly towards increasing cold tolerance. Evidence of proteins having a direct effect on cold tolerance is emerging but limited. With isolated protoplasts from warm...

journal_title：Plant molecular biology

authors： Newton SS,Duman JG

更新日期：2000-11-01 00:00:00

abstract：:We have isolated and characterized a cDNA from a strawberry fruit subtractive library that shows homology to class-I low-molecular-weight (LMW) heat-shock protein genes from other higher plants. The strawberry cDNA (clone njjs4) was a 779 bp full-length cDNA with a single open reading frame of 468 bp that is expected ...

journal_title：Plant molecular biology

authors： Medina-Escobar N,Cárdenas J,Muñoz-Blanco J,Caballero JL

更新日期：1998-01-01 00:00:00

abstract：:A 47 kDa glycoprotein, termed EP4, was purified from carrot cell suspension culture medium. An antiserum raised against EP4 also recognized a protein of 45 kDa that was ionically bound to the cell wall. EP4 was detected in culture media from both embryogenic and non-embryogenic cell lines and was found to be secreted ...

journal_title：Plant molecular biology

authors： van Engelen FA,de Jong AJ,Meijer EA,Kuil CW,Meyboom JK,Dirkse WG,Booij H,Hartog MV,Vandekerckhove J,de Vries SC

更新日期：1995-03-01 00:00:00

abstract：:Despite recent progress auxin signal transduction remains largely scetchy and enigmatic. A good body of evidence supports the notion that the ABP1 could be a functional receptor or part of a receptor, respectively, but this is not generally accepted. Evidence for other functional receptors is lacking, as is any clearc...

journal_title：Plant molecular biology

authors： Scherer GF

更新日期：2002-06-01 00:00:00

abstract：:Bacteroids of Rhizobium leguminosarum in root nodules of Pisum sativum are enclosed by a plant-derived peribacteriod membrane (PBM). The contents of the interstitial peribacteroid space (PBS) between bacteroid membrane and PBM were isolated by a controlled osmotic shock of PBM-enclosed bacteroids and analysed by two-d...

journal_title：Plant molecular biology

authors： Katinakis P,Lankhorst RM,Louwerse J,van Kammen A,van den Bos RC

更新日期：1988-03-01 00:00:00

abstract：:The expression of beta-amylase genes in rice (Oryza sativa) and its regulation by phytohormones gibberellic acid (GA) and abscisic acid (ABA) were examined. Upon germination beta-amylase is synthesized de novo in aleurone cells and (GA) is not required. Exogenous addition of GA does not enhance the beta-amylase activi...

journal_title：Plant molecular biology

authors： Wang SM,Lue WL,Eimert K,Chen J

更新日期：1996-08-01 00:00:00

abstract：:Telomeres, the complex nucleoprotein structures at the ends of linear eukaryotic chromosomes, along with telomerase, the enzyme that synthesizes telomeric DNA, are required to maintain a stable genome. Together, the enzyme and substrate perform this essential service by protecting chromosomes from exonucleolytic degra...

journal_title：Plant molecular biology

authors： McKnight TD,Riha K,Shippen DE

更新日期：2002-03-01 00:00:00

abstract：:Acyl-CoA binding proteins (ACBPs) are small (ca. 10 kDa) highly-conserved cytosolic proteins that bind long-chain acyl-CoAs. A novel cDNA encoding ACBP1, a predicted membrane protein of 24.1 kDa with an acyl-CoA binding protein domain at its carboxy terminus, was cloned from Arabidopsis thaliana. At this domain, ACBP1...

journal_title：Plant molecular biology

authors： Chye ML

更新日期：1998-11-01 00:00:00

abstract：:Galactomannans are hemicellulosic polysaccharides composed of a (1 → 4)-linked β-D-mannan backbone substituted with single-unit (1 → 6)-α-linked D-galactosyl residues. Developing fenugreek (Trigonella foenum-graecum) seeds are known to accumulate large quantities of galactomannans in the endosperm, and were thus used ...

journal_title：Plant molecular biology

authors： Wang Y,Alonso AP,Wilkerson CG,Keegstra K

更新日期：2012-06-01 00:00:00

abstract：:The location on the wheat chloroplast DNA map and the nucleotide sequences of the genes coding for tRNA GCC (Gly) (trnG-GCC), tRNA GUC (Asp) (trnD-GUC) and tRNA GCA (Cys) (trnC-GCA) have been determined. These three genes are located in the large single copy region of the chloroplast genome, about half-way between one...

journal_title：Plant molecular biology

authors： Quigley F,Grienenberger JM,Weil JH

更新日期：1985-09-01 00:00:00

abstract：:Heat shock protein 90 (Hsp90) is an abundant and highly conserved molecular chaperone. In Arabidopsis, the Hsp90 gene family consists of seven members. Here, we report that the AtHsp90-6 gene gives rise to two mRNA populations, termed AtHsp90-6L and AtHsp90-6S due to alternative initiation of transcription. The AtHsp9...

journal_title：Plant molecular biology

authors： Prassinos C,Haralampidis K,Milioni D,Samakovli D,Krambis K,Hatzopoulos P

更新日期：2008-07-01 00:00:00

abstract：:Tight control of cell/tissue identity is essential for a correct and functional organ patterning, an important component of overall fruit development and eventual maturation and ripening. Despite many investigations regarding the molecular determinants of cell identity in fruits of different species, a useful model ab...

journal_title：Plant molecular biology

authors： Botton A,Rasori A,Ziliotto F,Moing A,Maucourt M,Bernillon S,Deborde C,Petterle A,Varotto S,Bonghi C

更新日期：2016-05-01 00:00:00

abstract：KEY MESSAGE:OsHIRP1 is an E3 ligase that acts as a positive regulator in the plant response to heat stress, thus providing important information relating to adaptation and regulation under heat stress in plant. Extreme temperature adversely affects plant growth, development, and productivity. Here, we report the molecu...

journal_title：Plant molecular biology

authors： Kim JH,Lim SD,Jang CS

更新日期：2019-04-01 00:00:00

abstract：:Microtubules are thought to be major determinants of plant morphogenesis, through effects on planes of cell division and on directions of differential cell expansion. In differentiation and redifferentiation processes, tubulin expression may prove a useful early indicator of cell activity. We examined the expression a...

journal_title：Plant molecular biology

authors： Stotz HU,Long SR

更新日期：1999-11-01 00:00:00

abstract：:Arabidopsis thaliana encodes four Dicer-like (DCL) proteins and five dsRNA-binding (DRB) proteins. We have previously demonstrated that DCL4 specifically interacts with DRB4 in vitro. Here we describe the interaction between DCL4 and DRB4 in vivo. The phenotype of a mutant with a defect in DCL4 (dcl4-2) was similar to...

journal_title：Plant molecular biology

authors： Nakazawa Y,Hiraguri A,Moriyama H,Fukuhara T

更新日期：2007-04-01 00:00:00

abstract：:Brachypodium distachyon (Brachypodium) has been recently recognized as an emerging model system for both comparative and functional genomics in grass species. In this study, 55,221 repeat masked Brachypodium BAC end sequences (BES) were used for comparative analysis against the 12 rice pseudomolecules. The analysis re...

journal_title：Plant molecular biology

authors： Huo N,Vogel JP,Lazo GR,You FM,Ma Y,McMahon S,Dvorak J,Anderson OD,Luo MC,Gu YQ

更新日期：2009-05-01 00:00:00

abstract：:Cosmid binary vectors for the introduction of DNA into plant cells have been constructed. These vectors are derived from the replicon of the broad host range plasmid pRK2 and contain the T-DNA border regions between which have been placed a chimaeric gene conferring resistance to kanamycin in plant cells. Appropriate ...

journal_title：Plant molecular biology

authors： van den Elzen P,Lee KY,Townsend J,Bedbrook J

更新日期：1985-05-01 00:00:00

abstract：:To isolate genes which are expressed preferentially during embryogenesis, a Douglas-fir embryogenesis cDNA library was constructed and differentially screened with cDNA probes made with mRNA from developing and mature embryos, respectively. The cDNA clone PM 2.1 was isolated based on its abundance in developing seeds ...

journal_title：Plant molecular biology

authors： Chatthai M,Kaukinen KH,Tranbarger TJ,Gupta PK,Misra S

更新日期：1997-05-01 00:00:00