Abstract:
:Rice provides a staple source of energy, protein and other nutrients to half of the world population. Over 90 of the rice seeds consists of starch and protein by dry weight. The quantity and property of starch and protein thus play a dominant role in the yield and quality of rice. The amylase content of starch is a determining factor in the eating and cooking quality while the amount and essential amino acids balance of storage proteins affect the nutritional quality of rice. In China, the super-hybrid rice currently under the last phase of development has a 35 yield advantage over the best inbred rice varieties. However, its grain quality needs further improvement. This study reported the expression patterns of 44 genes participating in starch, storage protein, and lysine synthesis in the developing rice grain. Field grown rice cultivar 9311, the paternal line of an elite super-hybrid rice LYP9 with its draft genomic sequence released, was used as plant material. Results revealed diverse yet coordinated expression profiles of the genes involved in the three pathways which lead to the final composition and property of starch, protein and lysine that determine the quality of rice, providing useful information for rice quality improvement.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Duan M,Sun SSdoi
10.1007/s11103-004-7507-3subject
Has Abstractpub_date
2005-09-01 00:00:00pages
165-78issue
1eissn
0167-4412issn
1573-5028journal_volume
59pub_type
杂志文章abstract::During maize seed development, endosperm cells synthesize large amounts of storage proteins, alpha-, beta-, and gamma-zeins, which accumulate within endoplasmic reticulum (ER)-derived protein bodies. The absence of lysine in all zein polypeptides results in an imbalance in the amino acid composition of maize seeds. We...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005889314967
更新日期:1997-05-01 00:00:00
abstract::A partial cDNA clone (PSnir) encoding the C-terminal region of nitrite reductase was isolated from a lambda gt11 library of the gymnosperm Pimus sylvestris (L.). Nucleotide sequence analysis showed that PSnir contains a reading frame encoding 105 amino acid residues. The amino acid sequence revealed a homology to NiR ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00043873
更新日期:1994-06-01 00:00:00
abstract::Heat shock proteins (hsps) are synthesized at higher temperatures in soybean seedlings exposed to a gradual temperature increase as compared to a rapid heat shock (hs). There are differences in the pattern of synthesis of different hsps depending on whether the temperature exposure is rapid or gradual. The synthesis o...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020626
更新日期:1985-09-01 00:00:00
abstract::Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor composed of NF-YA, NF-YB and NF-YC proteins. In this study, we identified and characterized a gene, GmNFYA3, which encodes the NF-YA subunit of the NF-Y complex in soybeans (Glycine max L.). Real time RT-PCR analysis indicated that GmNFYA3 was induced by...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0040-5
更新日期:2013-05-01 00:00:00
abstract::The characterization of the operon encoding the alpha and beta subunits of rhodophytan (R)-phycoerythrin (PE) from the macrophytic red alga Polysiphonia boldii is reported. This plastid-encoded operon was cloned, its nucleotide sequence determined, and its expression characterized by northern and primer extension anal...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00039617
更新日期:1993-01-01 00:00:00
abstract::RNA b is the most abundant member of a family of autonomously replicating single- and double-stranded RNA plasmids found in maize mitochondria. The extent to which this molecule is associated with proteins was investigated by rate zonal and CsCl equilibrium density gradient centrifugation of clarified lysates of S cyt...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005818631401
更新日期:1997-06-01 00:00:00
abstract::In order to optimise expression of a foreign protein in transgenic plants we investigated the potential benefits of including a viral untranslated leader sequence within a plant transformation vector. A variety of 5 leaders, including the tobacco mosaic virus (TMV) leader sequence and 31 nucleotides of the cauliflower...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00021423
更新日期:1993-10-01 00:00:00
abstract::Arabidopsis thaliana encodes four Dicer-like (DCL) proteins and five dsRNA-binding (DRB) proteins. We have previously demonstrated that DCL4 specifically interacts with DRB4 in vitro. Here we describe the interaction between DCL4 and DRB4 in vivo. The phenotype of a mutant with a defect in DCL4 (dcl4-2) was similar to...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-9125-8
更新日期:2007-04-01 00:00:00
abstract::The full-length nucleotide sequence of a barley (Hordeum vulgare L.) leaf mRNA, found to increase rapidly in amount during infection attempts by the powdery mildew fungus (Erysiphe graminis DC. ex Mérat), is reported. The mRNA encodes a polypeptide of 809 amino acid residues which, by sequence comparison, was identifi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023606
更新日期:1993-03-01 00:00:00
abstract::A full-length cDNA encoding glutamine synthetase was isolated from a lambda gt11 library constructed from the poly(A)+ RNA isolated from lettuce seeds incubated under red light. The nucleotide sequence of the cDNA and the deduced sequence of amino acids showed a high degree of homology to those of the cytosol-type glu...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00036917
更新日期:1990-08-01 00:00:00
abstract::Using a 2,4-dichlorophenoxyacetic acid (2,4-D)-dependent tobacco cell-suspension culture we analyzed early hormone-induced molecular events preceding cell division. By differential screening of a cDNA library to mRNAs derived from hormone-starved cells treated with 2,4-D for 4 h, seven non-cross hybridizing cDNA clone...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00016152
更新日期:1987-03-01 00:00:00
abstract::The expression of a 25 kDa protein, encoded by the fused mitochondrial pcf gene, is associated with cytoplasmic male sterility (CMS) in petunia. To investigate the role of the 25 kDa protein in CMS we have transformed petunia and tobacco plants with constructs expressing a portion of the urfS sequence of the pcf cDNA ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00042040
更新日期:1995-04-01 00:00:00
abstract::The coding sequence for FLP recombinase, originally from the 2 mu plasmid of Saccharomyces cerevisiae, was introduced into Arabidopsis behind the cauliflower mosaic virus 35S promoter. FLP activity was monitored by the glucuronidase activity resulting from inversion of an antisense-oriented GUS reporter gene flanked b...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00032673
更新日期:1995-09-01 00:00:00
abstract:KEY MESSAGE:In response to an ultrasound pulse, several hundred DEGs, including in response to stress, were up- or down-regulated in in vitro potato plantlets. Despite this abiotic stress, plantlets survived. Ultrasound (US) can influence plant growth and development. To better understand the genetic mechanism underlyi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00876-0
更新日期:2019-07-01 00:00:00
abstract::We have cloned and sequenced a wound-inducible cDNA clone designated WIP1 (for wound-induced protein) from maize coleoptiles. It was isolated by differential screening of a cDNA library prepared from excised maize coleoptile segments. The deduced amino acid sequence predicts a secretory, cysteine-rich protein of 102 r...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027365
更新日期:1993-08-01 00:00:00
abstract::Several signal transduction pathways play important roles in the sexual life cycle of Chlamydomonas. Nitrogen deprivation, perhaps sensed as a drop in intracellular [NH4+], triggers a signal transduction pathway that results in altered gene expression and the induction of the gametogenic pathway. Blue light triggers a...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/BF00016474
更新日期:1994-12-01 00:00:00
abstract::Plants count on a wide variety of metabolic, physiological, and developmental responses to adapt their growth to variations in mineral nutrient availability. To react to such variations plants have evolved complex sensing and signaling mechanisms that allow them to monitor the external and internal concentration of ea...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-008-9380-y
更新日期:2009-03-01 00:00:00
abstract::Retroviruses and retrotransposons make up the broad class of retroelements replicating and transposing via reverse transcriptase. Retroelements have recently been found to be ubiquitous in the plants. We report here the isolation, sequence and analysis of a retroelement from barley (Hordeum vulgare L.) with all the fe...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027369
更新日期:1993-08-01 00:00:00
abstract::Two hevein-like peptides from the seed of Pharbitis nil, designated Pharbitis nil antimicrobial peptide 1 (Pn-AMP1) and Pn-AMP2, had been purified previously. Both exhibit potent in vitro antifungal activity against a broad spectrum of phytopathogenic fungi. We now report the isolation of two cDNA clones, designated p...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1019864222515
更新日期:2002-10-01 00:00:00
abstract::Glutelin is the most abundant storage protein in rice, which is expressed specifically in the endosperm of maturing seed. Glutelin is encoded by about 10 genes per haploid genome, which are clearly divided into two subfamilies (GluA and GluB). Most of them are coordinately expressed during seed maturation in spite of ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019553
更新日期:1996-03-01 00:00:00
abstract::GRAS proteins belong to a plant-specific transcription factor family. Currently, 33 GRAS members including a putative expressed pseudogene have been identified in the Arabidopsis genome. With a reverse genetic approach, we have constructed a "phenome-ready unimutant collection" of the GRAS genes in Arabidopsis thalian...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9345-1
更新日期:2008-08-01 00:00:00
abstract::Silicon (Si) confers several benefits to many plant species when absorbed as silicic acid through nodulin 26-like intrinsic proteins (NIPs). The NIPs belong to major intrinsic protein (MIP) family, members of which form channels with high selectivity to control transport of water and different solutes. Here, comparati...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0087-3
更新日期:2013-11-01 00:00:00
abstract::The identification of plant genes involved in early phases of in vitro morphogenesis can not only contribute to our understanding of the processes underlying growth regulator-controlled determination, but also provide novel markers for evaluating the outcome of in vitro regeneration experiments. To search for such gen...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020486
更新日期:1996-12-01 00:00:00
abstract:KEY MESSAGE:TaPSK5 is a less conserved target of miR164 in wheat encoding a positive regulator of root growth and yield traits that could be used for crop improvement. MicroRNAs (miRNAs) play key roles in regulating plant growth and development by targeting the mRNAs of conserved genes. However, little is known about t...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01064-1
更新日期:2020-12-01 00:00:00
abstract::Members of the plant-specific GRAS family of putative transcription factors are involved in various aspects of plant development. SCARECROW (SCR) is a member of this protein family and plays a significant role in the radial patterning of both roots and shoots. However, little is known about the regulation of SCR expre...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-0892-9
更新日期:2004-05-01 00:00:00
abstract::The sesame 2S albumin (2Salb) promoter was evaluated for its capacity to express the reporter gusA gene encoding β-glucuronidase in transgenic tobacco seeds relative to the soybean fad3C gene promoter element. Results revealed increased expression of gusA gene in tobacco seed tissue when driven by sesame 2S albumin pr...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0233-6
更新日期:2014-11-01 00:00:00
abstract::A cDNA library was made from poly(A)+ RNA isolated from developing Douglas fir (Pseudotsuga menziesii) embryo and megagametophytic tissue, and the cDNA clones were identified by immunoscreening with polyclonal antiserum against the crystalloid storage protein complex of Douglas fir. The nucleotide sequence of the long...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00014555
更新日期:1993-02-01 00:00:00
abstract::Free Air [CO(2)] Enrichment (FACE) allows for plant growth under fully open-air conditions of elevated [CO(2)] at concentrations expected to be reached by mid-century. We used Arabidopsis thaliana ecotypes Col-0, Cvi-0, and WS to analyze changes in gene expression and metabolite profiles of plants grown in "SoyFACE" (...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-9041-y
更新日期:2006-11-01 00:00:00
abstract::The stress-induced expression of four different ubiquitin-encoding cDNAs was characterized in potato tuber tissue. The four clones exhibited differences in both structure and expression. The first cDNA encoded a single ubiquitin unit fused to an 80 amino acid ribosomal extension protein identical to the extension prot...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00014491
更新日期:1992-10-01 00:00:00
abstract::We have cloned, sequenced, and examined the expression of genes from pine trees that appear to encode extracellular class II chitinase. Nucleotide sequence analysis indicates a coding sequence composed of three exons interrupted by two introns at locations identical to those found in other chitinase genes that possess...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005708611020
更新日期:1997-04-01 00:00:00