New potential markers of in vitro tomato morphogenesis identified by mRNA differential display.

Abstract:

:The identification of plant genes involved in early phases of in vitro morphogenesis can not only contribute to our understanding of the processes underlying growth regulator-controlled determination, but also provide novel markers for evaluating the outcome of in vitro regeneration experiments. To search for such genes and to monitor changes in gene expression accompanying in vitro regeneration, we have adapted the mRNA differential display technique to the comparative analysis of a model system of tomato cotyledons that can be driven selectively toward either shoot or callus formation by means of previously determined growth regulator supplementations. Hormone-independent transcriptional modulation (mainly down-regulation) has been found to be the most common event, indicating that a non-specific reprogramming of gene expression quantitatively predominates during the early phases of in vitro culture. However, cDNA fragments representative of genes that are either down-regulated or induced in a programme-specific manner could also be identified, and two of them (G35, G36) were further characterized. One of these cDNA fragments, G35, corresponds to an mRNA that is down-regulated much earlier in callus- (day 2) than in shoot-determined explants (day 6). The other, G36, identifies an mRNA that is transiently expressed in shoot-determined explants only, well before any macroscopic signs of differentiation become apparent, and thus exhibits typical features of a morphogenetic marker.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Torelli A,Soragni E,Bolchi A,Petrucco S,Ottonello S,Branca C

doi

10.1007/BF00020486

subject

Has Abstract

pub_date

1996-12-01 00:00:00

pages

891-900

issue

5

eissn

0167-4412

issn

1573-5028

journal_volume

32

pub_type

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