Methyl-coenzyme M reductase-dependent endogenous methane enhances plant tolerance against abiotic stress and alters ABA sensitivity in Arabidopsis thaliana.

Abstract:

KEY MESSAGE:Our study firstly elaborated the underlying mechanism of endogenous CH4-induced abiotic tolerance, along with an alteration of ABA sensitivity by mimicking the endogenous CH4 production in MtMCR transgenic Arabidopsis. Endogenous methane (CH4) production and/or emission have been ubiquitously observed in stressed plants. However, their physiological roles remain unclear. Here, the methyl-coenzyme M reductase gene from Methanobacterium thermoautotrophicum (MtMCR), encoding the enzyme of methanogenesis, was expressed in Arabidopsis thaliana, to mimic the production of endogenous CH4. In response to salinity and osmotic stress, MtMCR expression was up-regulated in transgenic plants, resulting in significant increase of endogenous CH4 levels. Similar results were observed in abscisic acid (ABA) treatment. The functions of endogenous CH4 were characterized by the changes in plant phenotypes related to stress and ABA sensitivity during the germination and post-germination periods. When challenged with osmotic stress, a reduction in water loss and stomatal closure, were observed. Redox homeostasis was reestablished during osmotic and salinity stress, and ion imbalance was also restored in salinity conditions. The expression of several stress/ABA-responsive genes was up-regulated, and ABA sensitivity, in particularly, was significantly altered in the MtMCR transgenic plants. Together, our genetic study for the first time elaborated the possible mechanism of endogenous CH4-enhanced salinity and osmotic tolerance, along with an alteration of ABA sensitivity. These findings thus provided novel cues for understanding the possible roles of endogenous CH4 in plants.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Su J,Yang X,He J,Zhang Y,Duan X,Wang R,Shen W

doi

10.1007/s11103-019-00914-x

subject

Has Abstract

pub_date

2019-11-01 00:00:00

pages

439-454

issue

4-5

eissn

0167-4412

issn

1573-5028

pii

10.1007/s11103-019-00914-x

journal_volume

101

pub_type

杂志文章
  • Markers for hypersensitive response and senescence show distinct patterns of expression.

    abstract::Controlled cellular suicide is an important process that can be observed in various organs during plant development. From the generation of proper sexual organs in monoecious plants to the hypersensitive response (HR) that occurs during incompatible pathogen interactions, programmed cell death (PCD) can be readily obs...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006133311402

    authors: Pontier D,Gan S,Amasino RM,Roby D,Lam E

    更新日期:1999-04-01 00:00:00

  • Synthesis versus degradation: directions of amino acid metabolism during Arabidopsis abiotic stress response.

    abstract:KEY MESSAGE:During abiotic stress low abundant amino acids are not synthesized but they accumulate due to increased protein turnover under conditions inducing carbohydrate starvation (dehydration, salt stress, darkness) and are degraded. Metabolic adaptation is crucial for abiotic stress resistance in plants, and accum...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-018-0767-0

    authors: Hildebrandt TM

    更新日期:2018-09-01 00:00:00

  • Characterisation of transcript initiation sites in ribosome-deficient barley plastids.

    abstract::Transcription of plastid genes in higher plants is driven by two RNA polymerases. One is encoded in the chloroplast, the other is encoded in the nucleus. RNA synthesis in ribosome-deficient plastids is performed exclusively by the nuclear-encoded enzyme. In vitro capping was used to identify the transcriptional start ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1005924502336

    authors: Hübschmann T,Börner T

    更新日期:1998-02-01 00:00:00

  • Characterization of topoisomerase I and II activities in nuclear extracts during callogenesis in immature embryos of Zea mays.

    abstract::We have characterized the topoisomerase I and II activities in nuclear extracts from immature embryos of Zea mays and the effect of the treatment with 2,4-dichlorophenoxyacetic acid (2,4-D) and abscisic acid (ABA). These extracts were shown to be essentially devoid of protease and nuclease activities and they were tes...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00017917

    authors: Carballo M,Giné R,Santos M,Puigdomènech P

    更新日期:1991-01-01 00:00:00

  • Large-scale analysis of the GRAS gene family in Arabidopsis thaliana.

    abstract::GRAS proteins belong to a plant-specific transcription factor family. Currently, 33 GRAS members including a putative expressed pseudogene have been identified in the Arabidopsis genome. With a reverse genetic approach, we have constructed a "phenome-ready unimutant collection" of the GRAS genes in Arabidopsis thalian...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-008-9345-1

    authors: Lee MH,Kim B,Song SK,Heo JO,Yu NI,Lee SA,Kim M,Kim DG,Sohn SO,Lim CE,Chang KS,Lee MM,Lim J

    更新日期:2008-08-01 00:00:00

  • Clones from a shooty tobacco crown gall tumor II: irregular T-DNA structures and organization, T-DNA methylation and conditional expression of opine genes.

    abstract::Transformed clones from a shooty tobacco crown gall tumor, induced byAgrobacterium tumefaciens strain LBA1501, having the auxin locus of the TL-region inactivated by a Tn1831 insertion, were investigated for their T-DNA structure and expression. It has been described previously (28) that in addition to clones with an ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00752901

    authors: Peerbolte R,Leenhouts K,Hooykaas-van Slogteren GM,Wullems GJ,Schilperoort RA

    更新日期:1986-07-01 00:00:00

  • RNA-Seq analysis and de novo transcriptome assembly of Hevea brasiliensis.

    abstract::Hevea brasiliensis, being the only source of commercial natural rubber, is an extremely economically important crop. In an effort to facilitate biological, biochemical and molecular research in rubber biosynthesis, here we report the use of next-generation massively parallel sequencing technologies and de novo transcr...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-011-9811-z

    authors: Xia Z,Xu H,Zhai J,Li D,Luo H,He C,Huang X

    更新日期:2011-10-01 00:00:00

  • NTR1 encodes a floral nectary-specific gene in Brassica campestris L. ssp. pekinensis.

    abstract::We have characterized a gene specifically expressed in the floral nectaries of Brassica campestris L. ssp. pekinensis. Differential screening led to the isolation of a floral nectary-specific cDNA clone. Northern hybridization indicated that its mRNA transcript is 1450 nucleotides long and specific to the flower base....

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006381625421

    authors: Song JT,Seo HS,Song SI,Lee JS,Choi YD

    更新日期:2000-03-01 00:00:00

  • Tomato protoplast DNA transformation: physical linkage and recombination of exogenous DNA sequences.

    abstract::Tomato protoplasts have been transformed with plasmid DNA's, containing a chimeric kanamycin resistance gene and putative tomato origins of replication. A calcium phosphate-DNA mediated transformation procedure was employed in combination with either polyethylene glycol or polyvinyl alcohol. There were no indications ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00015816

    authors: Jongsma M,Koornneef M,Zabel P,Hille J

    更新日期:1987-09-01 00:00:00

  • A novel E-type endo-beta-1,4-glucanase with a putative cellulose-binding domain is highly expressed in ripening strawberry fruits.

    abstract::Two full-length cDNA clones (faEG1 and faEG3, respectively) have been isolated by screening a cDNA library representing transcripts from red strawberry fruits. Southern blot analysis of genomic DNA suggests that the strawberry endo-beta-1,4-glucanases (EGases) are encoded by a multigene family. The cognate genes are p...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006299821980

    authors: Trainotti L,Spolaore S,Pavanello A,Baldan B,Casadoro G

    更新日期:1999-05-01 00:00:00

  • A pathogenesis related-10 protein CaARP functions as aldo/keto reductase to scavenge cytotoxic aldehydes.

    abstract::Pathogenesis related-10 (PR-10) proteins are present as multigene family in most of the higher plants. The role of PR-10 proteins in plant is poorly understood. A sequence analysis revealed that a large number of PR-10 proteins possess conserved motifs found in aldo/keto reductases (AKRs) of yeast and fungi. We took t...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-015-0405-z

    authors: Jain D,Khandal H,Khurana JP,Chattopadhyay D

    更新日期:2016-01-01 00:00:00

  • New potential markers of in vitro tomato morphogenesis identified by mRNA differential display.

    abstract::The identification of plant genes involved in early phases of in vitro morphogenesis can not only contribute to our understanding of the processes underlying growth regulator-controlled determination, but also provide novel markers for evaluating the outcome of in vitro regeneration experiments. To search for such gen...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00020486

    authors: Torelli A,Soragni E,Bolchi A,Petrucco S,Ottonello S,Branca C

    更新日期:1996-12-01 00:00:00

  • Pea lectin receptor-like kinase functions in salinity adaptation without yield penalty, by alleviating osmotic and ionic stresses and upregulating stress-responsive genes.

    abstract::Lectin receptor-like kinases (LecRLKs) are members of RLK family composed of lectin-like extracellular recognition domain, transmembrane domain and cytoplasmic kinase domain. LecRLKs are plasma membrane proteins believed to be involved in signal transduction. However, most of the members of the protein family even in ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-015-0319-9

    authors: Vaid N,Pandey P,Srivastava VK,Tuteja N

    更新日期:2015-05-01 00:00:00

  • Profiling methyl jasmonate-responsive transcriptome for understanding induced systemic resistance in whitebark pine (Pinus albicaulis).

    abstract:KEY MESSAGE:RNA-seq analysis on whitebark pine needles demonstrated that methyl jasmonate (MeJA)-triggered transcriptome re-programming substantially overlapped with defense responses against insects and fungal pathogens in Pinus species, increasing current knowledge regarding induced systemic resistance (ISR) to patho...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-017-0655-z

    authors: Liu JJ,Williams H,Li XR,Schoettle AW,Sniezko RA,Murray M,Zamany A,Roke G,Chen H

    更新日期:2017-11-01 00:00:00

  • The monomeric GTPase RabA2 is required for progression and maintenance of membrane integrity of infection threads during root nodule symbiosis.

    abstract:KEY MESSAGE:Progression of the infection canal that conducts rhizobia to the nodule primordium requires a functional Rab GTPase located in Golgi/trans-Golgi that also participate in root hair polar growth. Common bean (Phaseolus vulgaris) symbiotically associates with its partner Rhizobium etli, resulting in the format...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-016-0581-5

    authors: Dalla Via V,Traubenik S,Rivero C,Aguilar OM,Zanetti ME,Blanco FA

    更新日期:2017-04-01 00:00:00

  • Heterologous co-expression in E. coli of isoamylase genes from cassava Manihot esculenta Crantz 'KU50' achieves enzyme-active heteromeric complex formation.

    abstract:KEY MESSAGE:Cloning of two isoamylase genes, MeISA1 and MeISA2, from cassava (Manihot esculenta Crantz) tubers, accompanied by their co-expression in E. coli demonstrates a requirement for heteromeric complex formation to achieve debranching activity. Starch debranching enzyme (DBE) or isoamylase (ISA) (EC.3.2.1.68), a...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-018-0707-z

    authors: Panpetch P,Field RA,Limpaseni T

    更新日期:2018-03-01 00:00:00

  • Cloning and sequence analysis of a signal peptidase I from the thermophilic cyanobacterium Phormidium laminosum.

    abstract::Type I signal peptidases are a widespread family of enzymes which remove the presequences from proteins translocated across cell membranes, including thylakoid and cytoplasmic membranes of cyanobacteria and thylakoid membranes of chloroplasts. We have cloned and sequenced a signal peptidase gene from the thermophilic ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00019191

    authors: Packer JC,André D,Howe CJ

    更新日期:1995-01-01 00:00:00

  • Subtractive cDNA libraries identify differentially expressed genes in dormant and growing buds of leafy spurge (Euphorbia esula).

    abstract::Two subtractive cDNA libraries were developed to study genes associated with bud dormancy (reverse library) and initiation of shoot growth (forward library) in leafy spurge. To identify unique sequences represented in each library, 15744 clones were screened to reduce the level of redundancy within both libraries. A t...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-006-0015-x

    authors: Jia Y,Anderson JV,Horvath DP,Gu YQ,Lym RG,Chao WS

    更新日期:2006-05-01 00:00:00

  • Regulation of transcription by the Arabidopsis UVR8 photoreceptor involves a specific histone modification.

    abstract::The photoreceptor UV RESISTANCE LOCUS 8 (UVR8) specifically mediates photomorphogenic responses to UV-B wavelengths. UVR8 acts by regulating transcription of a set of genes, but the underlying mechanisms are unknown. Previous research indicated that UVR8 can associate with chromatin, but the specificity and functional...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-016-0522-3

    authors: Velanis CN,Herzyk P,Jenkins GI

    更新日期:2016-11-01 00:00:00

  • Specificity of auxin regulation of gibberellin 20-oxidase gene expression in pea pericarp.

    abstract::During early pea fruit growth, the physiological roles of 4-chloroindole-3-acetic acid (4-Cl-IAA) and IAA, natural pea auxins, in regulating gibberellin (GA) 20-oxidase gene expression (PsGA20ox1) were tested with 4-position, ring-substituted auxins that have a range of biological activities (fruit growth). The effect...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1015522404586

    authors: Ngo P,Ozga JA,Reinecke DM

    更新日期:2002-07-01 00:00:00

  • New patterns of gene activity in plants detected using an Agrobacterium vector.

    abstract::A vector has been designed that contains a truncated CaMV (cauliflower mosaic virus) 35S promoter fused to a receptor gene encoding beta-glucuronidase (GUS), placed adjacent to the left border sequence of an Agrobacterium vector. In potato plants transformed with this vector, different patterns of transcription were d...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00020557

    authors: Goldsbrough A,Bevan M

    更新日期:1991-02-01 00:00:00

  • Unraveling the functions of type II-prohibitins in Arabidopsis mitochondria.

    abstract::In yeast and mammals, prohibitins (PHBs) are considered as structural proteins that form a scaffold-like structure for interacting with a set of proteins involved in various processes occurring in the mitochondria. The role of PHB in plant mitochondria is poorly understood. In the study, the model organism Arabidopsis...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-015-0320-3

    authors: Piechota J,Bereza M,Sokołowska A,Suszyński K,Lech K,Jańska H

    更新日期:2015-06-01 00:00:00

  • Cloning of the glutamine synthetase gene fromspirulina platensis.

    abstract::An 8 Kilobase-pair (Kbp) HindIII fragment containing the coding sequence forSpirulina platensis glutamine synthetase [EC 6.3.1.1.] has been identified utilizing a probe derived fromAnabaena 7120 and cloned in the vector pAT153. ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF02418760

    authors: Riccardi G,De Rossi E,Della Valle G,Ciferri O

    更新日期:1985-03-01 00:00:00

  • Characterization of a cDNA encoding the protein moiety of a putative arabinogalactan protein from Lycopersicon esculentum.

    abstract::The nucleotide sequence of a cDNA prepared from poly(A)+ RNA from Lycopersicon esculentum fruit codes for a protein, M(r) 20,812, with features representative of the protein core of arabinogalactan proteins. The deduced amino acid sequence resembles that of peptides of arabinogalactan proteins isolated from carrot and...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00020254

    authors: Pogson BJ,Davies C

    更新日期:1995-05-01 00:00:00

  • PtSVP, an SVP homolog from trifoliate orange (Poncirus trifoliata L. Raf.), shows seasonal periodicity of meristem determination and affects flower development in transgenic Arabidopsis and tobacco plants.

    abstract::A MADS-box gene was isolated using the suppressive subtractive hybridization library between early-flowering mutant and wild-type trifoliate orange (Poncirus trifoliata L. Raf.). This gene is highly homologous with Arabidopsis SHORT VEGETATIVE PHASE (SVP). Based on real-time PCR and in situ hybridization during bud di...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-010-9660-1

    authors: Li ZM,Zhang JZ,Mei L,Deng XX,Hu CG,Yao JL

    更新日期:2010-09-01 00:00:00

  • Down-regulation of specific members of the glutamine synthetase gene family in alfalfa by antisense RNA technology.

    abstract::Glutamine synthetase (GS) catalyzes the ATP-dependent condensation of NH3 with glutamate to produce glutamine. In plants GS is an octameric enzyme and is located either in the cytoplasm (GS1) or in the chloroplast (GS2). Two distinct classes of GS1 genes with unique 3'-untranslated region (3'UTR) have been identified ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006099512706

    authors: Temple SJ,Bagga S,Sengupta-Gopalan C

    更新日期:1998-06-01 00:00:00

  • Curculin, a sweet-tasting and taste-modifying protein, is a non-functional mannose-binding lectin.

    abstract::A three-dimensional model of curculin, a sweet-tasting and taste-modifying protein from the fruits of Curculigo latifolia, was built from the X-ray coordinates of GNA, a mannose-binding lectin from snowdrop (Galanthus nivalis). The three mannose-binding sites present in GNA were found in curculin but are devoid of man...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1005704616565

    authors: Barre A,Van Damme EJ,Peumans WJ,Rougé P

    更新日期:1997-03-01 00:00:00

  • Phylogenetic distribution and genetic mapping of a (GGC)n microsatellite from rice (Oryza sativa L.).

    abstract::DNA microsatellites are ubiquitously present in eukaryotic genomes and represent a vast source of highly informative markers. We describe in this article a (GGC)n microsatellite which is widely distributed in eukaryotic genomes. Using polymerase chain reaction (PCR) techniques and DNA sequencing, we demonstrated for t...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00014544

    authors: Zhao X,Kochert G

    更新日期:1993-02-01 00:00:00

  • Degradation of the stress-responsive enzyme formate dehydrogenase by the RING-type E3 ligase Keep on Going and the ubiquitin 26S proteasome system.

    abstract:KEY MESSAGE:KEG is involved in mediating the proteasome-dependent degradation of FDH, a stress-responsive enzyme. The UPS may function to suppress FDH mediated stress responses under favorable growth conditions. Formate dehydrogenase (FDH) has been studied in bacteria and yeasts for the purpose of industrial applicatio...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-017-0691-8

    authors: McNeilly D,Schofield A,Stone SL

    更新日期:2018-02-01 00:00:00

  • WIP1, a wound-inducible gene from maize with homology to Bowman-Birk proteinase inhibitors.

    abstract::We have cloned and sequenced a wound-inducible cDNA clone designated WIP1 (for wound-induced protein) from maize coleoptiles. It was isolated by differential screening of a cDNA library prepared from excised maize coleoptile segments. The deduced amino acid sequence predicts a secretory, cysteine-rich protein of 102 r...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00027365

    authors: Rohrmeier T,Lehle L

    更新日期:1993-08-01 00:00:00