Tomato protoplast DNA transformation: physical linkage and recombination of exogenous DNA sequences.

abstract：:Osmium tetroxide (OsO4) reacts with the thymine residues of double-stranded DNA, but thymines that are unpaired or under torsional stress are hyperreactive. Although OsO4 hyperreactivity has been primarily utilized to identify Z-DNA structures in supercoiled plasmids, OsO4 will also identify other torsional perturbati...

journal_title：Plant molecular biology

authors： Paul AL,Ferl RJ

更新日期：1993-09-01 00:00:00

abstract：:We identified a novel nucleoplasm localized protein in Arabidopsis called AT-hook motif nuclear localized protein 1 (AHL1), which was isolated by visual screening of transformants using random GFP::cDNA fusions. AHL1 contains an AT-hook motif and unknown conserved PPC (plants and prokaryotes conserved) domain that inc...

journal_title：Plant molecular biology

authors： Fujimoto S,Matsunaga S,Yonemura M,Uchiyama S,Azuma T,Fukui K

更新日期：2004-09-01 00:00:00

abstract：:A gene family of at least five members encodes the tobacco mitochondrial Rieske Fe-S protein (RISP). To determine whether all five RISPs are translocated to mitochondria, fusion proteins containing the putative presequences of tobacco RISPs and Escherichia coli beta-glucuronidase (GUS) were expressed in transgenic tob...

journal_title：Plant molecular biology

authors： Huang J,Levings CS 3rd

更新日期：1995-11-01 00:00:00

abstract：:Unicellular algae grow well under limiting CO2 conditions, aided by a carbon concentrating mechanism (CCM). In C. reinhardtii, this mechanism is inducible and is present only in cells grown under low CO2 conditions. We constructed a cDNA library from cells adapting to low CO2, and screened the library for cDNAs specif...

journal_title：Plant molecular biology

authors： Burow MD,Chen ZY,Mouton TM,Moroney JV

更新日期：1996-05-01 00:00:00

abstract：:Transfer RNA genes have been mapped to at least nine different loci on the physical map of the Euglena gracilis chloroplast genome. One of these loci in the ribosomal RNA operons is present three times per genome. The DNA sequences of six of the nine different loci, containing 21 different tRNA genes, have been determ...

journal_title：Plant molecular biology

authors： Hallick RB,Hollingsworth MJ,Nickoloff JA

更新日期：1984-05-01 00:00:00

abstract：:Plants are capable to de novo synthesize the essential amino acids leucine, isoleucine and valine. Studies in recent years, however, also revealed that plants have the potential to degrade leucine or may be all of the branched-chain amino acids. One of the enzymes participating in both biosynthesis and degradation is ...

journal_title：Plant molecular biology

authors： Schuster J,Binder S

更新日期：2005-01-01 00:00:00

abstract：:Acyl carrier protein (ACP) is a key component of the fatty acid biosynthetic machinery in plants. A 1.4 kb 5' flanking region of a Brassica napus ACP gene (ACP05) was transcriptionally fused to the reporter gene beta-glucuronidase (GUS), and expression of the chimeric gene monitored in transgenic tobacco. GUS activity...

journal_title：Plant molecular biology

authors： de Silva J,Robinson SJ,Safford R

更新日期：1992-04-01 00:00:00

abstract：:It has been known for more than a decade that increasing the gene copy number does not necessarily lead to increased gene activity. Plants have developed efficient mechanisms such as post-transcriptional gene silencing (PTGS) to regulate abnormal gene expression in a sequence-specific fashion. PTGS of (trans)genes can...

journal_title：Plant molecular biology

authors： Morel JB,Vaucheret H

更新日期：2000-06-01 00:00:00

abstract：KEY MESSAGE:The crop yield losses induced by phytoviruses are mainly associated with the symptoms of the disease. DNA modifications as methylation can modulate the information coded by the sequence, process named epigenetics. Viral infection can change the expression patterns of different genes linked to defenses and s...

journal_title：Plant molecular biology

authors： Leone M,Zavallo D,Venturuzzi A,Asurmendi S

更新日期：2020-11-01 00:00:00

abstract：:In yeast, Rad21/Scc1 and its meiotic variant Rec8 are key players in the establishment and subsequent dissolution of sister chromatid cohesion for mitosis and meiosis, respectively, which are essential for chromosome segregation. Unlike yeast, our identification revealed that the rice genome has 4 RAD21-like genes tha...

journal_title：Plant molecular biology

authors： Zhang L,Tao J,Wang S,Chong K,Wang T

更新日期：2006-03-01 00:00:00

abstract：:cDNA clones encoding the precursor of the Rieske FeS protein of the chloroplast thylakoid membrane have been isolated from a pea leaf cDNA library in lambda gt 11, following screening with antibodies to purified pea chloroplast Rieske FeS protein. The longest cDNA insert of 880 bp encodes a polypeptide of 230 amino ac...

journal_title：Plant molecular biology

authors： Salter AH,Newman BJ,Napier JA,Gray JC

更新日期：1992-11-01 00:00:00

abstract：:The often rapid and synchronous programmed death of petal cells provides a model system to study molecular aspects of organ senescence. The death of petal cells is preceded by a loss of membrane permeability, due in part to increases in reactive oxygen species that are in turn related to up-regulation of oxidative enz...

journal_title：Plant molecular biology

authors： Rubinstein B

更新日期：2000-10-01 00:00:00

abstract：:A novel rice genomic sequence encoding coding segments homologous to other metallothionein-like genes was isolated from Oryza sativa genomic library. This sequence, hereby designated as rgMT (rice genomic metallothionein-like gene), consists of two exons and one intron. From the coding sequence, it is predicted that r...

journal_title：Plant molecular biology

authors： Hsieh HM,Liu WK,Huang PC

更新日期：1995-06-01 00:00:00

abstract：:The maize (Zea mays L.) caffeic acid O-methyl-transferase (COMT) is a key enzyme in the biosynthesis of lignin. In this work we have characterized the involvement of COMT in the lignification process through the study of the molecular mechanisms involved in its regulation. The examination of the maize COMT gene promot...

journal_title：Plant molecular biology

authors： Fornalé S,Sonbol FM,Maes T,Capellades M,Puigdomènech P,Rigau J,Caparrós-Ruiz D

更新日期：2006-12-01 00:00:00

abstract：:An antisense approach was used to attempt to determine the function of the highly abundant, tapetum-specific A9 transcript in microsporogenesis. A Brassica napus A9 cDNA clone was linked in sense and antisense orientations to the Arabidopsis thaliana A9 promoter and the resulting chimaeric genes introduced into B. nap...

journal_title：Plant molecular biology

authors： Turgut K,Barsby T,Craze M,Freeman J,Hodge R,Paul W,Scott R

更新日期：1994-01-01 00:00:00

abstract：:In the wake of recent progress of high throughput transcriptome profiling technologies, extensive housekeeping gene mining has been conducted in humans. However, very few studies have been reported in maize (Zea mays L.), an important crop plant, and none were conducted on a genome -wide level. In this study, we surve...

journal_title：Plant molecular biology

authors： Lin F,Jiang L,Liu Y,Lv Y,Dai H,Zhao H

更新日期：2014-11-01 00:00:00

abstract：:The full-length nucleotide sequence of a barley (Hordeum vulgare L.) leaf mRNA, found to increase rapidly in amount during infection attempts by the powdery mildew fungus (Erysiphe graminis DC. ex Mérat), is reported. The mRNA encodes a polypeptide of 809 amino acid residues which, by sequence comparison, was identifi...

journal_title：Plant molecular biology

authors： Walther-Larsen H,Brandt J,Collinge DB,Thordal-Christensen H

更新日期：1993-03-01 00:00:00

abstract：:In this study, we report the cloning of the SsCut gene encoding cutinase from Sclerotinia sclerotiorum. We isolated a 609-bp cDNA encoding a polypeptide of 202 amino acids with a molecular weight of 20.4 kDa. Heterologous expression of SsCut in Escherichia coli (His-SsCut) caused the formation of lesions in tobacco th...

journal_title：Plant molecular biology

authors： Zhang H,Wu Q,Cao S,Zhao T,Chen L,Zhuang P,Zhou X,Gao Z

更新日期：2014-11-01 00:00:00

abstract：:RNA editing of T-urf13, a gene specific to the mitochondria of cytoplasmic male-sterile, type-T (cms-T) maize, and an adjacent, cotranscribed gene orf221, have been studied by cDNA sequencing. No editing was detected in 22 cDNA clones. This is the only report of a polypeptide-encoding gene in higher-plant mitochondria...

journal_title：Plant molecular biology

authors： Ward GC,Levings CS 3rd

更新日期：1991-11-01 00:00:00

abstract：:Tomato plants stably expressing the tetR gene were transformed with the beta-glucuronidase (GUS) gene and an le20 antisense construct both under the control of the Triple-Op promoter. Induction of GUS mRNA and le20 antisense RNA in leaves was demonstrated in response to tetracycline. le20 antisense induction was also ...

journal_title：Plant molecular biology

authors： Thompson AJ,Myatt SC

更新日期：1997-07-01 00:00:00

abstract：:To understand the transcriptional responses to flooding stress in roots including hypocotyl of soybean seedlings, genome-wide changes in gene expression were analyzed using a soybean microarray chip containing 42,034 60-mer oligonucleotide probes. More than 6,000 of flooding-responsive genes in the roots including hyp...

journal_title：Plant molecular biology

authors： Nanjo Y,Maruyama K,Yasue H,Yamaguchi-Shinozaki K,Shinozaki K,Komatsu S

更新日期：2011-09-01 00:00:00

abstract：:The phytohormone abscisic acid is probably present in all higher plants. This hormone is necessary for regulation of several events during seed development and for the response to environmental stresses such as desiccation, salt and cold. An important part of the physiological response to abscisic acid is achieved thr...

journal_title：Plant molecular biology

authors： Busk PK,Pagès M

更新日期：1998-06-01 00:00:00

abstract：KEY MESSAGE:OsWRKY36 represses plant height and grain size by inhibiting gibberellin signaling. Plant height and grain size are important agronomic traits affecting yield in cereals, including rice. Gibberellins (GAs) are plant hormones that promote plant growth and developmental processions such as stem elongation and...

journal_title：Plant molecular biology

authors： Lan J,Lin Q,Zhou C,Ren Y,Liu X,Miao R,Jing R,Mou C,Nguyen T,Zhu X,Wang Q,Zhang X,Guo X,Liu S,Jiang L,Wan J

更新日期：2020-11-01 00:00:00

abstract：:Several clones corresponding to the gene encoding NADH-dependent hydroxypyruvate reductase have been isolated from a cucumber genomic library. Restriction mapping indicates the presence of two HPR genes, hpr-A and hpr-B, in the cucumber genome. Examination of the DNAs of individual plants suggests that hpr-A and hpr-B...

journal_title：Plant molecular biology

authors： Schwartz BW,Sloan JS,Becker WM

更新日期：1991-10-01 00:00:00

abstract：:Glycinebetaine is an important osmoprotectant in bacteria, plants, and animals, but only little information is available on the synthesis of glycinebetaine in tree plants. Among four mangrove species, glycinebetaine could be detected only in Avicennia marina. Pinitol was the main osmoprotectant in the other three spec...

journal_title：Plant molecular biology

authors： Hibino T,Meng YL,Kawamitsu Y,Uehara N,Matsuda N,Tanaka Y,Ishikawa H,Baba S,Takabe T,Wada K,Ishii T,Takabe T

更新日期：2001-02-01 00:00:00

abstract：:Knowledge of the genes in gymnosperms encoding the apoproteins of the plant photoreceptor phytochrome is currently scanty as for gymnosperm nuclear protein coding sequences in general. Here we report two complete cDNA-derived sequences which code for two different types of gymnosperm phytochrome. One sequence stems fr...

journal_title：Plant molecular biology

authors： Clapham DH,Kolukisaoglu HU,Larsson CT,Qamaruddin M,Ekberg I,Wiegmann-Eirund C,Schneider-Poetsch HA,von Arnold S

更新日期：1999-07-01 00:00:00

abstract：:Homologous recombination is an essential process for the maintenance and variability of the genome. In eukaryotes, the Rad52 epistasis group proteins serve the main role for meiotic recombination and/or homologous recombinational repair. Rad51-like proteins, such as Rad55 and Rad57 in yeast, play a role in assembly or...

journal_title：Plant molecular biology

authors： Osakabe K,Yoshioka T,Ichikawa H,Toki S

更新日期：2002-09-01 00:00:00

abstract：:This is the first report to describe the detailed analysis of TATA sequence requirements for the initiation of transcription for RNA polymerase II in a plant in vitro transcription system. We examined the transcriptional activities associated with 24 promoters, including wild-type (TATATATA), and the 21 point and two ...

journal_title：Plant molecular biology

authors： Yamaguchi Y,Itoh Y,Takeda Y,Yamazaki K

更新日期：1998-12-01 00:00:00

abstract：:The cytokinin gene, isopentenyl transferase (ipt), was placed under the control of a heat-inducible promoter from the Drosophila melanogaster hsp70 gene and introduced into Nicotiana plumbaginifolia by cocultivation with Agrobacterium tumefaciens. Transformants were analyzed for organ-specific expression, cytokinin le...

journal_title：Plant molecular biology

authors： Smigocki AC

更新日期：1991-01-01 00:00:00

abstract：:Salicylic acid (SA) is known to play an important role in the interaction between plant and micro-organisms, both symbiotic and pathogen. In particular, high levels of SA block nodule formation and mycorrhizal colonization in plants. A mutant of Lotus japonicus, named Ljsym4-2, was characterized as unable to establish...

journal_title：Plant molecular biology

authors： Bastianelli F,Costa A,Vescovi M,D'Apuzzo E,Zottini M,Chiurazzi M,Lo Schiavo F

更新日期：2010-03-01 00:00:00