Import of the precursor of the chloroplast Rieske iron-sulphur protein by pea chloroplasts.

Abstract:

:cDNA clones encoding the precursor of the Rieske FeS protein of the chloroplast thylakoid membrane have been isolated from a pea leaf cDNA library in lambda gt 11, following screening with antibodies to purified pea chloroplast Rieske FeS protein. The longest cDNA insert of 880 bp encodes a polypeptide of 230 amino acid residues, of which 50 residues constitute an N-terminal cleavable presequence and 180 residues make up the mature protein. Transcription and translation of the cDNA in vitro produced a polypeptide of 26 kDa which was efficiently imported by isolated pea chloroplasts and processed to the mature 20 kDa protein. Southern hybridisation to pea genomic DNA indicated the presence of a single gene encoding the Rieske FeS protein in the haploid genome.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Salter AH,Newman BJ,Napier JA,Gray JC

doi

10.1007/BF00040617

subject

Has Abstract

pub_date

1992-11-01 00:00:00

pages

569-74

issue

3

eissn

0167-4412

issn

1573-5028

journal_volume

20

pub_type

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