Generation of Arabidopsis protein chips for antibody and serum screening.

abstract：:The cis-regulatory elements that confer cell cycle-dependent expression to the wheat histone H3 gene were investigated in rice cells (Oc strain) transformed with H3/GUS chimeric genes. 5' deletion mutants of the H3 promoter region (from -1711, -908 or -185 to +57 relative to the transcription start site) were joined t...

journal_title：Plant molecular biology

authors： Ohtsubo N,Nakayama T,Terada R,Shimamoto K,Iwabuchi M

更新日期：1993-11-01 00:00:00

abstract：:We have characterized a gene specifically expressed in the floral nectaries of Brassica campestris L. ssp. pekinensis. Differential screening led to the isolation of a floral nectary-specific cDNA clone. Northern hybridization indicated that its mRNA transcript is 1450 nucleotides long and specific to the flower base....

journal_title：Plant molecular biology

authors： Song JT,Seo HS,Song SI,Lee JS,Choi YD

更新日期：2000-03-01 00:00:00

abstract：:Miniature inverted-repeat transposable elements (MITEs) are short, non autonomous DNA elements that are widespread and abundant in plant genomes. The high sequence and size conservation observed in many MITE families suggest that they have spread recently throughout their respective host genomes. Here we present a mai...

journal_title：Plant molecular biology

authors： Zerjal T,Joets J,Alix K,Grandbastien MA,Tenaillon MI

更新日期：2009-09-01 00:00:00

abstract：:Arabinogalactan-proteins (AGPs) are a family of complex proteoglycans found in all higher plants. Although the precise function(s) of any single AGP is unknown, they are implicated in diverse developmental roles such as differentiation, cell-cell recognition, embryogenesis and programmed cell death. DNA sequencing pro...

journal_title：Plant molecular biology

authors： Gaspar Y,Johnson KL,McKenna JA,Bacic A,Schultz CJ

更新日期：2001-09-01 00:00:00

abstract：:A CDNA encoding a 47 kDa nucleoside triphosphatase (NTPase) that is associated with the chromatin of pea nuclei has been cloned and sequenced. The translated sequence of the cDNA includes several domains predicted by known biochemical properties of the enzyme, including five motifs characteristic of the ATP-binding do...

journal_title：Plant molecular biology

authors： Hsieh HL,Tong CG,Thomas C,Roux SJ

更新日期：1996-01-01 00:00:00

abstract：:Coordinated cell growth and differentiation is crucial for the development of higher plants. Using the elongation defective 1-1 (eld1-1) mutant, we cloned the ELD1 gene, which encodes a serine-rich protein. Genes homologous to ELD1 can be found in plants, including Arabidopsis, rice, and tobacco, but not in other orga...

journal_title：Plant molecular biology

authors： Lertpiriyapong K,Sung ZR

更新日期：2003-11-01 00:00:00

abstract：:Alpha-dioxygenases (α-DOX) catalyzing the primary oxygenation of fatty acids to oxylipins were recently found in plants. Here, the biological roles of the pepper α-DOX (Ca-DOX) gene, which is strongly induced during non-host pathogen infection in chili pepper, were examined. Virus-induced gene silencing demonstrated t...

journal_title：Plant molecular biology

authors： Hong CE,Ha YI,Choi H,Moon JY,Lee J,Shin AY,Park CJ,Yoon GM,Kwon SY,Jo IH,Park JM

更新日期：2017-03-01 00:00:00

abstract：:The relative amounts of some chloroplast polypeptides were followed during greening of leaves from three different plant families. Oat, bean and spinach were the representatives of the Gramineae, Leguminosae and Chenopodiaceae, respectively. By using specific antibodies against subunits of the chloroplast protein comp...

journal_title：Plant molecular biology

authors： Nechushtai R,Nelson N

更新日期：1985-11-01 00:00:00

abstract：:Complex signal transduction pathways underlie the myriad plant responses to attack by pathogens. Ca(2+) is a universal second messenger in eukaryotes that modulates various signal transduction pathways through stimulus-specific changes in its intracellular concentration. Ca(2+)-binding proteins such as calmodulin (CaM...

journal_title：Plant molecular biology

authors： Chiasson D,Ekengren SK,Martin GB,Dobney SL,Snedden WA

更新日期：2005-08-01 00:00:00

abstract：:In this report, we describe the efficient expression and glycosylation, in insect cells, of β-phaseolin polypeptides (M r 45 and 48 kDa) from Phaseolus vulgaris, by means of a baculovirus expression vector. N-terminal sequence analysis demonstrated that the signal peptide was efficiently processed. Tunicamycin treatme...

journal_title：Plant molecular biology

authors： Bustos MM,Luckow VA,Griffing LR,Summers MD,Hall TC

更新日期：1988-11-01 00:00:00

abstract：:Microtubules are thought to be major determinants of plant morphogenesis, through effects on planes of cell division and on directions of differential cell expansion. In differentiation and redifferentiation processes, tubulin expression may prove a useful early indicator of cell activity. We examined the expression a...

journal_title：Plant molecular biology

authors： Stotz HU,Long SR

更新日期：1999-11-01 00:00:00

abstract：:RNA b is the most abundant member of a family of autonomously replicating single- and double-stranded RNA plasmids found in maize mitochondria. The extent to which this molecule is associated with proteins was investigated by rate zonal and CsCl equilibrium density gradient centrifugation of clarified lysates of S cyt...

journal_title：Plant molecular biology

authors： Formanová N,Brown GG

更新日期：1997-06-01 00:00:00

abstract：:Expression of the light-harvesting complex protein genes (Lhc) is under the control of a circadian clock. To dissect the molecular regulatory components of the circadian clock a promoter deletion analysis of four tomato Lhc genes was performed in transgenic tobacco plants. The important 5'-upstream promoter regions ar...

journal_title：Plant molecular biology

authors： Piechulla B,Merforth N,Rudolph B

更新日期：1998-11-01 00:00:00

abstract：:Alignment of transcripts and genome sequences yielded a set of alternatively spliced transcripts in four angiosperm genomes: three dicotyledon species Medicago truncatula (Medicago), Populus trichocarpa (poplar) and Arabidopsis thaliana (Arabidopsis), and the monocotyledon Oryzae sativa (rice). Intron retention was th...

journal_title：Plant molecular biology

authors： Baek JM,Han P,Iandolino A,Cook DR

更新日期：2008-07-01 00:00:00

abstract：:Ustilago maydis killer toxins are small polypeptides (7-14 kDa) which kill susceptible cells of closely related fungal species. The KP4 toxin is a single polypeptide subunit with a molecular weight of 11.1 kDa. In this work, a transgenic tobacco plant was constructed which secretes the KP4 toxin at a high level. The K...

journal_title：Plant molecular biology

authors： Park CM,Berry JO,Bruenn JA

更新日期：1996-01-01 00:00:00

abstract：:Glycine betaine (betaine) has the highest cellular osmoprotective efficiency which does not accumulate in most glycophytes. The biosynthetic pathway for betaine in higher plants is derived from the oxidation of low-accumulating metabolite choline that limiting the ability of most plants to produce betaine. Halophilic ...

journal_title：Plant molecular biology

authors： Lai SJ,Lai MC,Lee RJ,Chen YH,Yen HE

更新日期：2014-07-01 00:00:00

abstract：:DNA microsatellites are ubiquitously present in eukaryotic genomes and represent a vast source of highly informative markers. We describe in this article a (GGC)n microsatellite which is widely distributed in eukaryotic genomes. Using polymerase chain reaction (PCR) techniques and DNA sequencing, we demonstrated for t...

journal_title：Plant molecular biology

authors： Zhao X,Kochert G

更新日期：1993-02-01 00:00:00

abstract：:The synthesis of both strands of CaMV-DNA has been studied in vitro using viral replication complexes obtained by hypotonic extraction of infected plant organelles. Hybridization of the DNA synthesized in vitro to single stranded CaMV DNA probes cloned in bacteriophage M 13 confirmed that the 35 S RNA served as a temp...

journal_title：Plant molecular biology

authors： Pfeiffer P,Laquel P,Hohn T

更新日期：1984-09-01 00:00:00

abstract：:We studied the early events of de novo formation of adventitious shoot meristems in stem segments of Brassica oleracea. A regeneration system was used that is efficient, rapid, highly responsive to cytokinins and does not involve callus formation, thus allowing studies on a direct developmental switch of cells in the ...

journal_title：Plant molecular biology

authors： Teo WL,Kumar P,Goh CJ,Swarup S

更新日期：2001-07-01 00:00:00

abstract：KEY MESSAGE:Hybrid abnormalities, severe growth abortion and grass-clump dwarfism, were found in the tetraploid wheat/Aegilops umbellulata hybrids, and the gene expression changes were conserved in the hybrids with those in other wheat synthetic hexaploids. Aegilops umbellulata Zhuk., a diploid goatgrass species with a...

journal_title：Plant molecular biology

authors： Okada M,Yoshida K,Takumi S

更新日期：2017-12-01 00:00:00

abstract：:The complete nucleotide sequence of the rDNA large spacer region was determined for two related legume plants, Vicia faba and Pisum sativum. In the large spacers of both plants, there were one or several tandem arrays of repeated sequences which account well for the length heterogeneity of the rDNA repeating units. Wh...

journal_title：Plant molecular biology

authors： Kato A,Nakajima T,Yamashita J,Yakura K,Tanifuji S

更新日期：1990-06-01 00:00:00

abstract：:A cDNA encoding the multifunctional cytochrome P450, CYP71E1, involved in the biosynthesis of the cyanogenic glucoside dhurrin from Sorghum bicolor (L.) Moench was isolated. A PCR approach based on three consensus sequences of A-type cytochromes P450- (V/I)KEX(L/F)R, FXPERF, and PFGXGRRXCXG-was applied. Three novel cy...

journal_title：Plant molecular biology

authors： Bak S,Kahn RA,Nielsen HL,Moller BL,Halkier BA

更新日期：1998-02-01 00:00:00

abstract：:H(+)-ATPase cDNAs were identified in a potato leaf library using an Arabidopsis gene as a probe. Based on their sequences, the clones could be grouped into at least two classes. A similar classification was obtained from the analysis of sequence data from four tobacco genes. Both potato genes are expressed in all tiss...

journal_title：Plant molecular biology

authors： Harms K,Wöhner RV,Schulz B,Frommer WB

更新日期：1994-11-01 00:00:00

abstract：:Using RNA from developing cotyledons and leaves of bean two products were synthesizedin vitro, with Mr 31000 and 31500 which are antigenically related to the subunit of phytoferritin (Mr 26500). The relative abundance of the two products is dependent on the type of tissue from which the RNA was derived. Evidence is pr...

journal_title：Plant molecular biology

authors： van der Mark F,Klerk H,Bienfait F

更新日期：1983-11-01 00:00:00

abstract：:A leaf undergoes determinate growth from a primordium on flank of the shoot apical meristem. Several intrinsic pathways restrict meristematic activity in the leaf of Arabidopsis; however, other factors remain to be defined. We report here that the overexpression of MINI ZINC FINGER1 (MIF1) or MIF3 disrupted the leaf d...

journal_title：Plant molecular biology

authors： Hu W,Feng B,Ma H

更新日期：2011-05-01 00:00:00

abstract：:Two cDNA clones encoding fucoxanthin chlorophyll a/c-binding proteins (FCP) in the diatom Odontella sinensis have been cloned and sequenced. The derived amino acid sequences of both clones are identical, comparison of the corresponding nucleic acids reveals differences only in the third codon position, suggesting a re...

journal_title：Plant molecular biology

authors： Kroth-Pancic PG

更新日期：1995-02-01 00:00:00

abstract：:Eukaryotes express several cytoplasmic HSP70 genes, and their encoded proteins participate in diverse cellular processes. Three cDNAs encoding highly expressed cytoplasmic HSP70 homologues from Pisum sativum were cloned and characterized. They were designated PsHSP71.2, PsHSC71.0, and PsHSP70b. These HSP70 genes have ...

journal_title：Plant molecular biology

authors： DeRocher A,Vierling E

更新日期：1995-02-01 00:00:00

abstract：:The 12-oxo-phytodienoic acid reductases (OPRs) are enzymes that catalyze the reduction of double bonds adjacent to an oxo group in alpha,beta-unsaturated aldehydes or ketones. Some of them have very high substrate specificity and are part of the octadecanoid pathway which convert linolenic acid to the phytohormone jas...

journal_title：Plant molecular biology

authors： Zhang J,Simmons C,Yalpani N,Crane V,Wilkinson H,Kolomiets M

更新日期：2005-09-01 00:00:00

abstract：:Isoprene synthase (ISPS) catalyzes the formation of isoprene, an important volatile terpenoid with strong effects on global atmospheric chemistry and protective physiological functions in plant leaves. Many terpene synthase genes including isoprene synthase, a member of the TPS-b cluster of this numerous gene family, ...

journal_title：Plant molecular biology

authors： Cinege G,Louis S,Hänsch R,Schnitzler JP

更新日期：2009-03-01 00:00:00

abstract：:TPD (tapping panel dryness) is a complex physiological syndrome widely found in rubber tree (Hevea brasiliensis) plantations, which causes severe yield and crop losses in natural rubber-producing countries. The molecular mechanism underlying TPD is not known and there is presently no effective prevention or treatment ...

journal_title：Plant molecular biology

authors： Chen S,Peng S,Huang G,Wu K,Fu X,Chen Z

更新日期：2003-01-01 00:00:00